The Experts below are selected from a list of 198 Experts worldwide ranked by ideXlab platform

A Bach - One of the best experts on this subject based on the ideXlab platform.

  • short communication comparison of ph volatile fatty acids and microbiome of rumen samples from preweaned calves obtained via cannula or Stomach Tube
    Journal of Dairy Science, 2013
    Co-Authors: M Terre, L Castells, F Fabregas, A Bach
    Abstract:

    Abstract The objective of this study was to compare rumen samples from young dairy calves obtained via a Stomach Tube (ST) or a ruminal cannula (RC). Five male Holstein calves (46±4.0kg of body weight and 11±4.9 d of age) were ruminally cannulated at 15 d of age. Calves received 4 L/d of a commercial milk replacer (25% crude protein and 19.2% fat) at 12.5% dry matter, and were provided concentrate and chopped oats hay ad libitum throughout the study (56 d). In total, 29 paired rumen samples were obtained weekly throughout the study in most of the calves by each extraction method. These samples were used to determine pH and volatile fatty acids (VFA) concentration, and to quantify Prevotella ruminicola and Streptococcus bovis by quantitative PCR. Furthermore, a denaturing gradient gel electrophoresis was performed on rumen samples harvested during wk 8 of the study to determine the degree of similarity between rumen bacteria communities. Rumen pH was 0.30 units greater in ST compared with RC samples. Furthermore, total VFA concentrations were greater in RC than in ST samples. However, when analyzing the proportion of each VFA by ANOVA, no differences were found between the sampling methods. The quantification of S. bovis and P. ruminicola was similar in both extraction methods, and values obtained using different methods were highly correlated (R 2 =0.89 and 0.98 for S. bovis and P. ruminicola , respectively). Fingerprinting analysis showed similar bacteria band profiles between samples obtained from the same calves using different extraction methods. In conclusion, when comparing rumen parameters obtained using different sampling techniques, it is recommended that VFA profiles be used rather than total VFA concentrations, as total VFA concentrations are more affected by the method of collection. Furthermore, although comparisons of pH across studies should be avoided when samples are not obtained using the same sampling method, the comparison of fingerprinting of a bacteria community or a specific rumen bacterium is valid.

M Terre - One of the best experts on this subject based on the ideXlab platform.

  • short communication comparison of ph volatile fatty acids and microbiome of rumen samples from preweaned calves obtained via cannula or Stomach Tube
    Journal of Dairy Science, 2013
    Co-Authors: M Terre, L Castells, F Fabregas, A Bach
    Abstract:

    Abstract The objective of this study was to compare rumen samples from young dairy calves obtained via a Stomach Tube (ST) or a ruminal cannula (RC). Five male Holstein calves (46±4.0kg of body weight and 11±4.9 d of age) were ruminally cannulated at 15 d of age. Calves received 4 L/d of a commercial milk replacer (25% crude protein and 19.2% fat) at 12.5% dry matter, and were provided concentrate and chopped oats hay ad libitum throughout the study (56 d). In total, 29 paired rumen samples were obtained weekly throughout the study in most of the calves by each extraction method. These samples were used to determine pH and volatile fatty acids (VFA) concentration, and to quantify Prevotella ruminicola and Streptococcus bovis by quantitative PCR. Furthermore, a denaturing gradient gel electrophoresis was performed on rumen samples harvested during wk 8 of the study to determine the degree of similarity between rumen bacteria communities. Rumen pH was 0.30 units greater in ST compared with RC samples. Furthermore, total VFA concentrations were greater in RC than in ST samples. However, when analyzing the proportion of each VFA by ANOVA, no differences were found between the sampling methods. The quantification of S. bovis and P. ruminicola was similar in both extraction methods, and values obtained using different methods were highly correlated (R 2 =0.89 and 0.98 for S. bovis and P. ruminicola , respectively). Fingerprinting analysis showed similar bacteria band profiles between samples obtained from the same calves using different extraction methods. In conclusion, when comparing rumen parameters obtained using different sampling techniques, it is recommended that VFA profiles be used rather than total VFA concentrations, as total VFA concentrations are more affected by the method of collection. Furthermore, although comparisons of pH across studies should be avoided when samples are not obtained using the same sampling method, the comparison of fingerprinting of a bacteria community or a specific rumen bacterium is valid.

F Fabregas - One of the best experts on this subject based on the ideXlab platform.

  • short communication comparison of ph volatile fatty acids and microbiome of rumen samples from preweaned calves obtained via cannula or Stomach Tube
    Journal of Dairy Science, 2013
    Co-Authors: M Terre, L Castells, F Fabregas, A Bach
    Abstract:

    Abstract The objective of this study was to compare rumen samples from young dairy calves obtained via a Stomach Tube (ST) or a ruminal cannula (RC). Five male Holstein calves (46±4.0kg of body weight and 11±4.9 d of age) were ruminally cannulated at 15 d of age. Calves received 4 L/d of a commercial milk replacer (25% crude protein and 19.2% fat) at 12.5% dry matter, and were provided concentrate and chopped oats hay ad libitum throughout the study (56 d). In total, 29 paired rumen samples were obtained weekly throughout the study in most of the calves by each extraction method. These samples were used to determine pH and volatile fatty acids (VFA) concentration, and to quantify Prevotella ruminicola and Streptococcus bovis by quantitative PCR. Furthermore, a denaturing gradient gel electrophoresis was performed on rumen samples harvested during wk 8 of the study to determine the degree of similarity between rumen bacteria communities. Rumen pH was 0.30 units greater in ST compared with RC samples. Furthermore, total VFA concentrations were greater in RC than in ST samples. However, when analyzing the proportion of each VFA by ANOVA, no differences were found between the sampling methods. The quantification of S. bovis and P. ruminicola was similar in both extraction methods, and values obtained using different methods were highly correlated (R 2 =0.89 and 0.98 for S. bovis and P. ruminicola , respectively). Fingerprinting analysis showed similar bacteria band profiles between samples obtained from the same calves using different extraction methods. In conclusion, when comparing rumen parameters obtained using different sampling techniques, it is recommended that VFA profiles be used rather than total VFA concentrations, as total VFA concentrations are more affected by the method of collection. Furthermore, although comparisons of pH across studies should be avoided when samples are not obtained using the same sampling method, the comparison of fingerprinting of a bacteria community or a specific rumen bacterium is valid.

L Castells - One of the best experts on this subject based on the ideXlab platform.

  • short communication comparison of ph volatile fatty acids and microbiome of rumen samples from preweaned calves obtained via cannula or Stomach Tube
    Journal of Dairy Science, 2013
    Co-Authors: M Terre, L Castells, F Fabregas, A Bach
    Abstract:

    Abstract The objective of this study was to compare rumen samples from young dairy calves obtained via a Stomach Tube (ST) or a ruminal cannula (RC). Five male Holstein calves (46±4.0kg of body weight and 11±4.9 d of age) were ruminally cannulated at 15 d of age. Calves received 4 L/d of a commercial milk replacer (25% crude protein and 19.2% fat) at 12.5% dry matter, and were provided concentrate and chopped oats hay ad libitum throughout the study (56 d). In total, 29 paired rumen samples were obtained weekly throughout the study in most of the calves by each extraction method. These samples were used to determine pH and volatile fatty acids (VFA) concentration, and to quantify Prevotella ruminicola and Streptococcus bovis by quantitative PCR. Furthermore, a denaturing gradient gel electrophoresis was performed on rumen samples harvested during wk 8 of the study to determine the degree of similarity between rumen bacteria communities. Rumen pH was 0.30 units greater in ST compared with RC samples. Furthermore, total VFA concentrations were greater in RC than in ST samples. However, when analyzing the proportion of each VFA by ANOVA, no differences were found between the sampling methods. The quantification of S. bovis and P. ruminicola was similar in both extraction methods, and values obtained using different methods were highly correlated (R 2 =0.89 and 0.98 for S. bovis and P. ruminicola , respectively). Fingerprinting analysis showed similar bacteria band profiles between samples obtained from the same calves using different extraction methods. In conclusion, when comparing rumen parameters obtained using different sampling techniques, it is recommended that VFA profiles be used rather than total VFA concentrations, as total VFA concentrations are more affected by the method of collection. Furthermore, although comparisons of pH across studies should be avoided when samples are not obtained using the same sampling method, the comparison of fingerprinting of a bacteria community or a specific rumen bacterium is valid.

G Blanc - One of the best experts on this subject based on the ideXlab platform.

  • pharmacokinetics of a discontinuous absorption process of oxolinic acid in turbot scophthalmus maximus after a single oral administration
    Xenobiotica, 1998
    Co-Authors: I Poher, G Blanc
    Abstract:

    1. The pharmacokinetics of oxolinic acid have been studied in 500 g turbot (Scoph thalmus maximus). The fish were kept in seawater at 16 °C with a 15 h/9 h photoperiod. Oxolinic acid was administered orally via a Stomach Tube at a single dose of 10 mg/kg of body weight. Serum concentrations of oxolinic acid were determined by a (HPLC) using liquid phase extraction with an internal standard and a fluorescence detection. 2. The pharmacokinetic process was not significantly sex-influenced. The short elimination phase of the oxolinic acid in turbot after oral administration was similar to the elimination after intravascular administration. The serum concentration profile of oxolinic acid was better described by a discontinuous absorption model than by compartment models using continuous absorption processes. The absorption of oxolinic acid in turbot was characterized by two distinct phases after a lag time of about 2 h. A time (Tmax) of 12 h max was necessary to reach the peak serum concentration (Cmax) of 1....