The Experts below are selected from a list of 14115 Experts worldwide ranked by ideXlab platform
Klaus E Matzel - One of the best experts on this subject based on the ideXlab platform.
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dna Stool Test for colorectal cancer hypermethylation of the secreted frizzled related protein 1 gene
Diseases of The Colon & Rectum, 2007Co-Authors: Wei Zhang, Michael Bauer, Roland S Croner, Jorg Pelz, Dimitri Lodygin, Heiko Hermeking, Michael Sturzl, W Hohenberger, Klaus E MatzelAbstract:PURPOSE: To investigate a potential mode of noninvasive screening for colorectal cancer, we evaluated the hypermethylation of the secreted frizzled-related protein-1 gene promoter in human Stool DNA. METHODS: In Stool samples from 36 patients with colorectal neoplasia (7 adenoma, 29 colorectal cancer) and 17 healthy control subjects, isolated DNA was treated with sodium bisulfite and analyzed by methylation-specific polymerase chain reaction with primers specific for methylated or unmethylated promoter sequences of the secreted frizzled-related protein-1 gene. RESULTS: Hypermethylation of the secreted frizzled-related protein-1 promoter was present in the Stool DNA of patients with adenoma and colorectal cancer. A sensitivity of 89 percent and specificity of 86 percent were achieved in the detection of colorectal neoplasia. The difference in hypermethylation status of the secreted frizzled-related protein-1 promoter between the patients with colorectal neoplasia and the control group was statistically highly significant (P <0.001). Adenoma and early tumor Stage I (International Union Against Cancer) displayed both unmethylated and methylated secreted frizzled-related protein-1 promoter sequences, whereas advanced tumor stages showed only methylated secreted frizzled-related protein-1 (P = 0.05). CONCLUSIONS: The results indicate that this DNA Stool Test of hypermethylation of the secreted frizzled-related protein-1 promoter is a sensitive and specific method. It has the potential of a clinically useful Test for the early detection of colorectal cancer.
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DNA Stool Test for colorectal cancer: hypermethylation of the secreted frizzled-related protein-1 gene.
Diseases of the colon and rectum, 2007Co-Authors: Wei Zhang, Michael Bauer, Roland S Croner, Jorg Pelz, Dimitri Lodygin, Heiko Hermeking, Michael Sturzl, W Hohenberger, Klaus E MatzelAbstract:PURPOSE: To investigate a potential mode of noninvasive screening for colorectal cancer, we evaluated the hypermethylation of the secreted frizzled-related protein-1 gene promoter in human Stool DNA. METHODS: In Stool samples from 36 patients with colorectal neoplasia (7 adenoma, 29 colorectal cancer) and 17 healthy control subjects, isolated DNA was treated with sodium bisulfite and analyzed by methylation-specific polymerase chain reaction with primers specific for methylated or unmethylated promoter sequences of the secreted frizzled-related protein-1 gene. RESULTS: Hypermethylation of the secreted frizzled-related protein-1 promoter was present in the Stool DNA of patients with adenoma and colorectal cancer. A sensitivity of 89 percent and specificity of 86 percent were achieved in the detection of colorectal neoplasia. The difference in hypermethylation status of the secreted frizzled-related protein-1 promoter between the patients with colorectal neoplasia and the control group was statistically highly significant (P
Miklós Sahin-tóth - One of the best experts on this subject based on the ideXlab platform.
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Detection of human elastase isoforms by the ScheBo Pancreatic Elastase 1 Test
American Journal of Physiology-gastrointestinal and Liver Physiology, 2017Co-Authors: Anna Zsófia Tóth, Eszter Hegyi, Peter Hegyi, András Szabó, Miklós Sahin-tóthAbstract:The ScheBo Pancreatic Elastase 1 Stool Test is widely used to assess pancreatic exocrine function, yet its molecular targets have been poorly defined. We demonstrate that, among the human pancreatic proteinases, the Test measures the elastase isoform chymotrypsin-like elastase (CELA) 3B (CELA3B) and, to a lesser extent, CELA3A. Genetic variants of the human CELA3 isoforms have no significant effect on Test performance.
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Detection of human elastase isoforms by the ScheBo Pancreatic Elastase 1 Test
American journal of physiology. Gastrointestinal and liver physiology, 2017Co-Authors: Anna Zsófia Tóth, Eszter Hegyi, Peter Hegyi, András Szabó, Miklós Sahin-tóthAbstract:The ScheBo Pancreatic Elastase 1 Stool Test is widely used to assess pancreatic exocrine function, yet its molecular targets have been poorly defined. We demonstrate that, among the human pancreati...
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Detection of human elastase isoforms by the ScheBo Pancreatic Elastase 1 Test
American Journal of Physiology-gastrointestinal and Liver Physiology, 2017Co-Authors: Anna Zsófia Tóth, Eszter Hegyi, Peter Hegyi, András Szabó, Miklós Sahin-tóthAbstract:The ScheBo Pancreatic Elastase 1 Stool Test is widely used to assess pancreatic exocrine function, yet its molecular targets have been poorly defined. We demonstrate that, among the human pancreatic proteinases, the Test measures the elastase isoform chymotrypsin-like elastase (CELA) 3B (CELA3B) and, to a lesser extent, CELA3A. Genetic variants of the human CELA3 isoforms have no significant effect on Test performance.
Wei Zhang - One of the best experts on this subject based on the ideXlab platform.
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dna Stool Test for colorectal cancer hypermethylation of the secreted frizzled related protein 1 gene
Diseases of The Colon & Rectum, 2007Co-Authors: Wei Zhang, Michael Bauer, Roland S Croner, Jorg Pelz, Dimitri Lodygin, Heiko Hermeking, Michael Sturzl, W Hohenberger, Klaus E MatzelAbstract:PURPOSE: To investigate a potential mode of noninvasive screening for colorectal cancer, we evaluated the hypermethylation of the secreted frizzled-related protein-1 gene promoter in human Stool DNA. METHODS: In Stool samples from 36 patients with colorectal neoplasia (7 adenoma, 29 colorectal cancer) and 17 healthy control subjects, isolated DNA was treated with sodium bisulfite and analyzed by methylation-specific polymerase chain reaction with primers specific for methylated or unmethylated promoter sequences of the secreted frizzled-related protein-1 gene. RESULTS: Hypermethylation of the secreted frizzled-related protein-1 promoter was present in the Stool DNA of patients with adenoma and colorectal cancer. A sensitivity of 89 percent and specificity of 86 percent were achieved in the detection of colorectal neoplasia. The difference in hypermethylation status of the secreted frizzled-related protein-1 promoter between the patients with colorectal neoplasia and the control group was statistically highly significant (P <0.001). Adenoma and early tumor Stage I (International Union Against Cancer) displayed both unmethylated and methylated secreted frizzled-related protein-1 promoter sequences, whereas advanced tumor stages showed only methylated secreted frizzled-related protein-1 (P = 0.05). CONCLUSIONS: The results indicate that this DNA Stool Test of hypermethylation of the secreted frizzled-related protein-1 promoter is a sensitive and specific method. It has the potential of a clinically useful Test for the early detection of colorectal cancer.
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DNA Stool Test for colorectal cancer: hypermethylation of the secreted frizzled-related protein-1 gene.
Diseases of the colon and rectum, 2007Co-Authors: Wei Zhang, Michael Bauer, Roland S Croner, Jorg Pelz, Dimitri Lodygin, Heiko Hermeking, Michael Sturzl, W Hohenberger, Klaus E MatzelAbstract:PURPOSE: To investigate a potential mode of noninvasive screening for colorectal cancer, we evaluated the hypermethylation of the secreted frizzled-related protein-1 gene promoter in human Stool DNA. METHODS: In Stool samples from 36 patients with colorectal neoplasia (7 adenoma, 29 colorectal cancer) and 17 healthy control subjects, isolated DNA was treated with sodium bisulfite and analyzed by methylation-specific polymerase chain reaction with primers specific for methylated or unmethylated promoter sequences of the secreted frizzled-related protein-1 gene. RESULTS: Hypermethylation of the secreted frizzled-related protein-1 promoter was present in the Stool DNA of patients with adenoma and colorectal cancer. A sensitivity of 89 percent and specificity of 86 percent were achieved in the detection of colorectal neoplasia. The difference in hypermethylation status of the secreted frizzled-related protein-1 promoter between the patients with colorectal neoplasia and the control group was statistically highly significant (P
Heiko Hermeking - One of the best experts on this subject based on the ideXlab platform.
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dna Stool Test for colorectal cancer hypermethylation of the secreted frizzled related protein 1 gene
Diseases of The Colon & Rectum, 2007Co-Authors: Wei Zhang, Michael Bauer, Roland S Croner, Jorg Pelz, Dimitri Lodygin, Heiko Hermeking, Michael Sturzl, W Hohenberger, Klaus E MatzelAbstract:PURPOSE: To investigate a potential mode of noninvasive screening for colorectal cancer, we evaluated the hypermethylation of the secreted frizzled-related protein-1 gene promoter in human Stool DNA. METHODS: In Stool samples from 36 patients with colorectal neoplasia (7 adenoma, 29 colorectal cancer) and 17 healthy control subjects, isolated DNA was treated with sodium bisulfite and analyzed by methylation-specific polymerase chain reaction with primers specific for methylated or unmethylated promoter sequences of the secreted frizzled-related protein-1 gene. RESULTS: Hypermethylation of the secreted frizzled-related protein-1 promoter was present in the Stool DNA of patients with adenoma and colorectal cancer. A sensitivity of 89 percent and specificity of 86 percent were achieved in the detection of colorectal neoplasia. The difference in hypermethylation status of the secreted frizzled-related protein-1 promoter between the patients with colorectal neoplasia and the control group was statistically highly significant (P <0.001). Adenoma and early tumor Stage I (International Union Against Cancer) displayed both unmethylated and methylated secreted frizzled-related protein-1 promoter sequences, whereas advanced tumor stages showed only methylated secreted frizzled-related protein-1 (P = 0.05). CONCLUSIONS: The results indicate that this DNA Stool Test of hypermethylation of the secreted frizzled-related protein-1 promoter is a sensitive and specific method. It has the potential of a clinically useful Test for the early detection of colorectal cancer.
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DNA Stool Test for colorectal cancer: hypermethylation of the secreted frizzled-related protein-1 gene.
Diseases of the colon and rectum, 2007Co-Authors: Wei Zhang, Michael Bauer, Roland S Croner, Jorg Pelz, Dimitri Lodygin, Heiko Hermeking, Michael Sturzl, W Hohenberger, Klaus E MatzelAbstract:PURPOSE: To investigate a potential mode of noninvasive screening for colorectal cancer, we evaluated the hypermethylation of the secreted frizzled-related protein-1 gene promoter in human Stool DNA. METHODS: In Stool samples from 36 patients with colorectal neoplasia (7 adenoma, 29 colorectal cancer) and 17 healthy control subjects, isolated DNA was treated with sodium bisulfite and analyzed by methylation-specific polymerase chain reaction with primers specific for methylated or unmethylated promoter sequences of the secreted frizzled-related protein-1 gene. RESULTS: Hypermethylation of the secreted frizzled-related protein-1 promoter was present in the Stool DNA of patients with adenoma and colorectal cancer. A sensitivity of 89 percent and specificity of 86 percent were achieved in the detection of colorectal neoplasia. The difference in hypermethylation status of the secreted frizzled-related protein-1 promoter between the patients with colorectal neoplasia and the control group was statistically highly significant (P
Michael Bauer - One of the best experts on this subject based on the ideXlab platform.
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dna Stool Test for colorectal cancer hypermethylation of the secreted frizzled related protein 1 gene
Diseases of The Colon & Rectum, 2007Co-Authors: Wei Zhang, Michael Bauer, Roland S Croner, Jorg Pelz, Dimitri Lodygin, Heiko Hermeking, Michael Sturzl, W Hohenberger, Klaus E MatzelAbstract:PURPOSE: To investigate a potential mode of noninvasive screening for colorectal cancer, we evaluated the hypermethylation of the secreted frizzled-related protein-1 gene promoter in human Stool DNA. METHODS: In Stool samples from 36 patients with colorectal neoplasia (7 adenoma, 29 colorectal cancer) and 17 healthy control subjects, isolated DNA was treated with sodium bisulfite and analyzed by methylation-specific polymerase chain reaction with primers specific for methylated or unmethylated promoter sequences of the secreted frizzled-related protein-1 gene. RESULTS: Hypermethylation of the secreted frizzled-related protein-1 promoter was present in the Stool DNA of patients with adenoma and colorectal cancer. A sensitivity of 89 percent and specificity of 86 percent were achieved in the detection of colorectal neoplasia. The difference in hypermethylation status of the secreted frizzled-related protein-1 promoter between the patients with colorectal neoplasia and the control group was statistically highly significant (P <0.001). Adenoma and early tumor Stage I (International Union Against Cancer) displayed both unmethylated and methylated secreted frizzled-related protein-1 promoter sequences, whereas advanced tumor stages showed only methylated secreted frizzled-related protein-1 (P = 0.05). CONCLUSIONS: The results indicate that this DNA Stool Test of hypermethylation of the secreted frizzled-related protein-1 promoter is a sensitive and specific method. It has the potential of a clinically useful Test for the early detection of colorectal cancer.
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DNA Stool Test for colorectal cancer: hypermethylation of the secreted frizzled-related protein-1 gene.
Diseases of the colon and rectum, 2007Co-Authors: Wei Zhang, Michael Bauer, Roland S Croner, Jorg Pelz, Dimitri Lodygin, Heiko Hermeking, Michael Sturzl, W Hohenberger, Klaus E MatzelAbstract:PURPOSE: To investigate a potential mode of noninvasive screening for colorectal cancer, we evaluated the hypermethylation of the secreted frizzled-related protein-1 gene promoter in human Stool DNA. METHODS: In Stool samples from 36 patients with colorectal neoplasia (7 adenoma, 29 colorectal cancer) and 17 healthy control subjects, isolated DNA was treated with sodium bisulfite and analyzed by methylation-specific polymerase chain reaction with primers specific for methylated or unmethylated promoter sequences of the secreted frizzled-related protein-1 gene. RESULTS: Hypermethylation of the secreted frizzled-related protein-1 promoter was present in the Stool DNA of patients with adenoma and colorectal cancer. A sensitivity of 89 percent and specificity of 86 percent were achieved in the detection of colorectal neoplasia. The difference in hypermethylation status of the secreted frizzled-related protein-1 promoter between the patients with colorectal neoplasia and the control group was statistically highly significant (P
