The Experts below are selected from a list of 54 Experts worldwide ranked by ideXlab platform

Brian J Wilkinson - One of the best experts on this subject based on the ideXlab platform.

  • growth environment dependent modulation of staphylococcus aureus branched chain to straight chain Fatty Acid ratio and incorporation of unsaturated Fatty Acids
    PLOS ONE, 2016
    Co-Authors: Suranjana Sen, Seth R Johnson, Yang Song, Sirisha Sirobhushanam, Ryan Tefft, Craig Gatto, Brian J Wilkinson
    Abstract:

    The Fatty Acid composition of membrane glycerolipids is a major determinant of Staphylococcus aureus membrane biophysical properties that impacts key factors in cell physiology including susceptibility to membrane active antimicrobials, pathogenesis, and response to environmental stress. The Fatty Acids of S. aureus are considered to be a mixture of branched-chain Fatty Acids (BCFAs), which increase membrane fluidity, and Straight-Chain Fatty Acids (SCFAs) that decrease it. The balance of BCFAs and SCFAs in USA300 strain JE2 and strain SH1000 was affected considerably by differences in the conventional laboratory medium in which the strains were grown with media such as Mueller-Hinton broth and Luria broth resulting in high BCFAs and low SCFAs, whereas growth in Tryptic Soy Broth and Brain-Heart Infusion broth led to reduction in BCFAs and an increase in SCFAs. Straight-Chain unsaturated Fatty Acids (SCUFAs) were not detected. However, when S. aureus was grown ex vivo in serum, the Fatty Acid composition was radically different with SCUFAs, which increase membrane fluidity, making up a substantial proportion of the total ( 37%) and BCFAs (>36%) making up the rest. Staphyloxanthin, an additional major membrane lipid component unique to S. aureus, tended to be greater in content in cells with high BCFAs or SCUFAs. Cells with high staphyloxanthin content had a lower membrane fluidity that was attributed to increased production of staphyloxanthin. S. aureus saves energy and carbon by utilizing host Fatty Acids for part of its total Fatty Acids when growing in serum, which may impact biophysical properties and pathogenesis given the role of SCUFAs in virulence. The nutritional environment in which S. aureus is grown in vitro or in vivo in an infection is likely to be a major determinant of membrane Fatty Acid composition.

  • growth environment dependent modulation of staphylococcus aureus branched chain to straight chain Fatty Acid ratio and incorporation of unsaturated Fatty Acids
    bioRxiv, 2016
    Co-Authors: Suranjana Sen, Seth R Johnson, Yang Song, Sirisha Sirobhushanam, Ryan Tefft, Craig Gatto, Brian J Wilkinson
    Abstract:

    The Fatty Acid composition of membrane glycerolipids is a major determinant of Staphylococcus aureus membrane biophysical properties that impacts key factors in cell physiology including susceptibility to membrane active antimicrobials, pathogenesis, and response to environmental stress. The Fatty Acids of S. aureus are considered to be a mixture of branched-chain Fatty Acids (BCFAs), which increase membrane fluidity, and Straight-Chain Fatty Acids (SCFAs) that decrease it. The balance of BCFAs and SCFAs in strains USA300 and SH1000 was affected considerably by differences in the conventional laboratory medium in which the strains were grown with media such as Mueller-Hinton broth and Luria broth resulting in high BCFAs and low SCFAs, whereas growth in Tryptic Soy Broth and Brain-Heart Infusion broth led to reduction in BCFAs and an increase in SCFAs. Straight-Chain unsaturated Fatty Acids (SCUFAs) were not detected. However, when the organism was grown ex vivo in serum, the Fatty Acid composition was radically different with SCUFAs, which increase membrane fluidity, making up a substantial proportion of the total ( 37%) and BCFAs (>36%) making up the rest. Staphyloxanthin, an additional major membrane lipid component unique to S. aureus, tended to be greater in content in cells with high BCFAs or SCUFAs. Cells with high staphyloxanthin content had a lower membrane fluidity that was attributed to increased production of staphyloxanthin. S. aureus saves energy and carbon by utilizing host Fatty Acids for part of its total Fatty Acids when growing in serum. The Fatty Acid composition of in vitro grown S. aureus is likely to be a poor reflection of the Fatty Acid composition and biophysical properties of the membrane when the organism is growing in an infection in view of the role of SCUFAs in staphylococcal membrane composition and virulence.

Suranjana Sen - One of the best experts on this subject based on the ideXlab platform.

  • growth environment dependent modulation of staphylococcus aureus branched chain to straight chain Fatty Acid ratio and incorporation of unsaturated Fatty Acids
    PLOS ONE, 2016
    Co-Authors: Suranjana Sen, Seth R Johnson, Yang Song, Sirisha Sirobhushanam, Ryan Tefft, Craig Gatto, Brian J Wilkinson
    Abstract:

    The Fatty Acid composition of membrane glycerolipids is a major determinant of Staphylococcus aureus membrane biophysical properties that impacts key factors in cell physiology including susceptibility to membrane active antimicrobials, pathogenesis, and response to environmental stress. The Fatty Acids of S. aureus are considered to be a mixture of branched-chain Fatty Acids (BCFAs), which increase membrane fluidity, and Straight-Chain Fatty Acids (SCFAs) that decrease it. The balance of BCFAs and SCFAs in USA300 strain JE2 and strain SH1000 was affected considerably by differences in the conventional laboratory medium in which the strains were grown with media such as Mueller-Hinton broth and Luria broth resulting in high BCFAs and low SCFAs, whereas growth in Tryptic Soy Broth and Brain-Heart Infusion broth led to reduction in BCFAs and an increase in SCFAs. Straight-Chain unsaturated Fatty Acids (SCUFAs) were not detected. However, when S. aureus was grown ex vivo in serum, the Fatty Acid composition was radically different with SCUFAs, which increase membrane fluidity, making up a substantial proportion of the total ( 37%) and BCFAs (>36%) making up the rest. Staphyloxanthin, an additional major membrane lipid component unique to S. aureus, tended to be greater in content in cells with high BCFAs or SCUFAs. Cells with high staphyloxanthin content had a lower membrane fluidity that was attributed to increased production of staphyloxanthin. S. aureus saves energy and carbon by utilizing host Fatty Acids for part of its total Fatty Acids when growing in serum, which may impact biophysical properties and pathogenesis given the role of SCUFAs in virulence. The nutritional environment in which S. aureus is grown in vitro or in vivo in an infection is likely to be a major determinant of membrane Fatty Acid composition.

  • growth environment dependent modulation of staphylococcus aureus branched chain to straight chain Fatty Acid ratio and incorporation of unsaturated Fatty Acids
    bioRxiv, 2016
    Co-Authors: Suranjana Sen, Seth R Johnson, Yang Song, Sirisha Sirobhushanam, Ryan Tefft, Craig Gatto, Brian J Wilkinson
    Abstract:

    The Fatty Acid composition of membrane glycerolipids is a major determinant of Staphylococcus aureus membrane biophysical properties that impacts key factors in cell physiology including susceptibility to membrane active antimicrobials, pathogenesis, and response to environmental stress. The Fatty Acids of S. aureus are considered to be a mixture of branched-chain Fatty Acids (BCFAs), which increase membrane fluidity, and Straight-Chain Fatty Acids (SCFAs) that decrease it. The balance of BCFAs and SCFAs in strains USA300 and SH1000 was affected considerably by differences in the conventional laboratory medium in which the strains were grown with media such as Mueller-Hinton broth and Luria broth resulting in high BCFAs and low SCFAs, whereas growth in Tryptic Soy Broth and Brain-Heart Infusion broth led to reduction in BCFAs and an increase in SCFAs. Straight-Chain unsaturated Fatty Acids (SCUFAs) were not detected. However, when the organism was grown ex vivo in serum, the Fatty Acid composition was radically different with SCUFAs, which increase membrane fluidity, making up a substantial proportion of the total ( 37%) and BCFAs (>36%) making up the rest. Staphyloxanthin, an additional major membrane lipid component unique to S. aureus, tended to be greater in content in cells with high BCFAs or SCUFAs. Cells with high staphyloxanthin content had a lower membrane fluidity that was attributed to increased production of staphyloxanthin. S. aureus saves energy and carbon by utilizing host Fatty Acids for part of its total Fatty Acids when growing in serum. The Fatty Acid composition of in vitro grown S. aureus is likely to be a poor reflection of the Fatty Acid composition and biophysical properties of the membrane when the organism is growing in an infection in view of the role of SCUFAs in staphylococcal membrane composition and virulence.

Ryan Tefft - One of the best experts on this subject based on the ideXlab platform.

  • growth environment dependent modulation of staphylococcus aureus branched chain to straight chain Fatty Acid ratio and incorporation of unsaturated Fatty Acids
    PLOS ONE, 2016
    Co-Authors: Suranjana Sen, Seth R Johnson, Yang Song, Sirisha Sirobhushanam, Ryan Tefft, Craig Gatto, Brian J Wilkinson
    Abstract:

    The Fatty Acid composition of membrane glycerolipids is a major determinant of Staphylococcus aureus membrane biophysical properties that impacts key factors in cell physiology including susceptibility to membrane active antimicrobials, pathogenesis, and response to environmental stress. The Fatty Acids of S. aureus are considered to be a mixture of branched-chain Fatty Acids (BCFAs), which increase membrane fluidity, and Straight-Chain Fatty Acids (SCFAs) that decrease it. The balance of BCFAs and SCFAs in USA300 strain JE2 and strain SH1000 was affected considerably by differences in the conventional laboratory medium in which the strains were grown with media such as Mueller-Hinton broth and Luria broth resulting in high BCFAs and low SCFAs, whereas growth in Tryptic Soy Broth and Brain-Heart Infusion broth led to reduction in BCFAs and an increase in SCFAs. Straight-Chain unsaturated Fatty Acids (SCUFAs) were not detected. However, when S. aureus was grown ex vivo in serum, the Fatty Acid composition was radically different with SCUFAs, which increase membrane fluidity, making up a substantial proportion of the total ( 37%) and BCFAs (>36%) making up the rest. Staphyloxanthin, an additional major membrane lipid component unique to S. aureus, tended to be greater in content in cells with high BCFAs or SCUFAs. Cells with high staphyloxanthin content had a lower membrane fluidity that was attributed to increased production of staphyloxanthin. S. aureus saves energy and carbon by utilizing host Fatty Acids for part of its total Fatty Acids when growing in serum, which may impact biophysical properties and pathogenesis given the role of SCUFAs in virulence. The nutritional environment in which S. aureus is grown in vitro or in vivo in an infection is likely to be a major determinant of membrane Fatty Acid composition.

  • growth environment dependent modulation of staphylococcus aureus branched chain to straight chain Fatty Acid ratio and incorporation of unsaturated Fatty Acids
    bioRxiv, 2016
    Co-Authors: Suranjana Sen, Seth R Johnson, Yang Song, Sirisha Sirobhushanam, Ryan Tefft, Craig Gatto, Brian J Wilkinson
    Abstract:

    The Fatty Acid composition of membrane glycerolipids is a major determinant of Staphylococcus aureus membrane biophysical properties that impacts key factors in cell physiology including susceptibility to membrane active antimicrobials, pathogenesis, and response to environmental stress. The Fatty Acids of S. aureus are considered to be a mixture of branched-chain Fatty Acids (BCFAs), which increase membrane fluidity, and Straight-Chain Fatty Acids (SCFAs) that decrease it. The balance of BCFAs and SCFAs in strains USA300 and SH1000 was affected considerably by differences in the conventional laboratory medium in which the strains were grown with media such as Mueller-Hinton broth and Luria broth resulting in high BCFAs and low SCFAs, whereas growth in Tryptic Soy Broth and Brain-Heart Infusion broth led to reduction in BCFAs and an increase in SCFAs. Straight-Chain unsaturated Fatty Acids (SCUFAs) were not detected. However, when the organism was grown ex vivo in serum, the Fatty Acid composition was radically different with SCUFAs, which increase membrane fluidity, making up a substantial proportion of the total ( 37%) and BCFAs (>36%) making up the rest. Staphyloxanthin, an additional major membrane lipid component unique to S. aureus, tended to be greater in content in cells with high BCFAs or SCUFAs. Cells with high staphyloxanthin content had a lower membrane fluidity that was attributed to increased production of staphyloxanthin. S. aureus saves energy and carbon by utilizing host Fatty Acids for part of its total Fatty Acids when growing in serum. The Fatty Acid composition of in vitro grown S. aureus is likely to be a poor reflection of the Fatty Acid composition and biophysical properties of the membrane when the organism is growing in an infection in view of the role of SCUFAs in staphylococcal membrane composition and virulence.

Craig Gatto - One of the best experts on this subject based on the ideXlab platform.

  • growth environment dependent modulation of staphylococcus aureus branched chain to straight chain Fatty Acid ratio and incorporation of unsaturated Fatty Acids
    PLOS ONE, 2016
    Co-Authors: Suranjana Sen, Seth R Johnson, Yang Song, Sirisha Sirobhushanam, Ryan Tefft, Craig Gatto, Brian J Wilkinson
    Abstract:

    The Fatty Acid composition of membrane glycerolipids is a major determinant of Staphylococcus aureus membrane biophysical properties that impacts key factors in cell physiology including susceptibility to membrane active antimicrobials, pathogenesis, and response to environmental stress. The Fatty Acids of S. aureus are considered to be a mixture of branched-chain Fatty Acids (BCFAs), which increase membrane fluidity, and Straight-Chain Fatty Acids (SCFAs) that decrease it. The balance of BCFAs and SCFAs in USA300 strain JE2 and strain SH1000 was affected considerably by differences in the conventional laboratory medium in which the strains were grown with media such as Mueller-Hinton broth and Luria broth resulting in high BCFAs and low SCFAs, whereas growth in Tryptic Soy Broth and Brain-Heart Infusion broth led to reduction in BCFAs and an increase in SCFAs. Straight-Chain unsaturated Fatty Acids (SCUFAs) were not detected. However, when S. aureus was grown ex vivo in serum, the Fatty Acid composition was radically different with SCUFAs, which increase membrane fluidity, making up a substantial proportion of the total ( 37%) and BCFAs (>36%) making up the rest. Staphyloxanthin, an additional major membrane lipid component unique to S. aureus, tended to be greater in content in cells with high BCFAs or SCUFAs. Cells with high staphyloxanthin content had a lower membrane fluidity that was attributed to increased production of staphyloxanthin. S. aureus saves energy and carbon by utilizing host Fatty Acids for part of its total Fatty Acids when growing in serum, which may impact biophysical properties and pathogenesis given the role of SCUFAs in virulence. The nutritional environment in which S. aureus is grown in vitro or in vivo in an infection is likely to be a major determinant of membrane Fatty Acid composition.

  • growth environment dependent modulation of staphylococcus aureus branched chain to straight chain Fatty Acid ratio and incorporation of unsaturated Fatty Acids
    bioRxiv, 2016
    Co-Authors: Suranjana Sen, Seth R Johnson, Yang Song, Sirisha Sirobhushanam, Ryan Tefft, Craig Gatto, Brian J Wilkinson
    Abstract:

    The Fatty Acid composition of membrane glycerolipids is a major determinant of Staphylococcus aureus membrane biophysical properties that impacts key factors in cell physiology including susceptibility to membrane active antimicrobials, pathogenesis, and response to environmental stress. The Fatty Acids of S. aureus are considered to be a mixture of branched-chain Fatty Acids (BCFAs), which increase membrane fluidity, and Straight-Chain Fatty Acids (SCFAs) that decrease it. The balance of BCFAs and SCFAs in strains USA300 and SH1000 was affected considerably by differences in the conventional laboratory medium in which the strains were grown with media such as Mueller-Hinton broth and Luria broth resulting in high BCFAs and low SCFAs, whereas growth in Tryptic Soy Broth and Brain-Heart Infusion broth led to reduction in BCFAs and an increase in SCFAs. Straight-Chain unsaturated Fatty Acids (SCUFAs) were not detected. However, when the organism was grown ex vivo in serum, the Fatty Acid composition was radically different with SCUFAs, which increase membrane fluidity, making up a substantial proportion of the total ( 37%) and BCFAs (>36%) making up the rest. Staphyloxanthin, an additional major membrane lipid component unique to S. aureus, tended to be greater in content in cells with high BCFAs or SCUFAs. Cells with high staphyloxanthin content had a lower membrane fluidity that was attributed to increased production of staphyloxanthin. S. aureus saves energy and carbon by utilizing host Fatty Acids for part of its total Fatty Acids when growing in serum. The Fatty Acid composition of in vitro grown S. aureus is likely to be a poor reflection of the Fatty Acid composition and biophysical properties of the membrane when the organism is growing in an infection in view of the role of SCUFAs in staphylococcal membrane composition and virulence.

Sirisha Sirobhushanam - One of the best experts on this subject based on the ideXlab platform.

  • growth environment dependent modulation of staphylococcus aureus branched chain to straight chain Fatty Acid ratio and incorporation of unsaturated Fatty Acids
    PLOS ONE, 2016
    Co-Authors: Suranjana Sen, Seth R Johnson, Yang Song, Sirisha Sirobhushanam, Ryan Tefft, Craig Gatto, Brian J Wilkinson
    Abstract:

    The Fatty Acid composition of membrane glycerolipids is a major determinant of Staphylococcus aureus membrane biophysical properties that impacts key factors in cell physiology including susceptibility to membrane active antimicrobials, pathogenesis, and response to environmental stress. The Fatty Acids of S. aureus are considered to be a mixture of branched-chain Fatty Acids (BCFAs), which increase membrane fluidity, and Straight-Chain Fatty Acids (SCFAs) that decrease it. The balance of BCFAs and SCFAs in USA300 strain JE2 and strain SH1000 was affected considerably by differences in the conventional laboratory medium in which the strains were grown with media such as Mueller-Hinton broth and Luria broth resulting in high BCFAs and low SCFAs, whereas growth in Tryptic Soy Broth and Brain-Heart Infusion broth led to reduction in BCFAs and an increase in SCFAs. Straight-Chain unsaturated Fatty Acids (SCUFAs) were not detected. However, when S. aureus was grown ex vivo in serum, the Fatty Acid composition was radically different with SCUFAs, which increase membrane fluidity, making up a substantial proportion of the total ( 37%) and BCFAs (>36%) making up the rest. Staphyloxanthin, an additional major membrane lipid component unique to S. aureus, tended to be greater in content in cells with high BCFAs or SCUFAs. Cells with high staphyloxanthin content had a lower membrane fluidity that was attributed to increased production of staphyloxanthin. S. aureus saves energy and carbon by utilizing host Fatty Acids for part of its total Fatty Acids when growing in serum, which may impact biophysical properties and pathogenesis given the role of SCUFAs in virulence. The nutritional environment in which S. aureus is grown in vitro or in vivo in an infection is likely to be a major determinant of membrane Fatty Acid composition.

  • growth environment dependent modulation of staphylococcus aureus branched chain to straight chain Fatty Acid ratio and incorporation of unsaturated Fatty Acids
    bioRxiv, 2016
    Co-Authors: Suranjana Sen, Seth R Johnson, Yang Song, Sirisha Sirobhushanam, Ryan Tefft, Craig Gatto, Brian J Wilkinson
    Abstract:

    The Fatty Acid composition of membrane glycerolipids is a major determinant of Staphylococcus aureus membrane biophysical properties that impacts key factors in cell physiology including susceptibility to membrane active antimicrobials, pathogenesis, and response to environmental stress. The Fatty Acids of S. aureus are considered to be a mixture of branched-chain Fatty Acids (BCFAs), which increase membrane fluidity, and Straight-Chain Fatty Acids (SCFAs) that decrease it. The balance of BCFAs and SCFAs in strains USA300 and SH1000 was affected considerably by differences in the conventional laboratory medium in which the strains were grown with media such as Mueller-Hinton broth and Luria broth resulting in high BCFAs and low SCFAs, whereas growth in Tryptic Soy Broth and Brain-Heart Infusion broth led to reduction in BCFAs and an increase in SCFAs. Straight-Chain unsaturated Fatty Acids (SCUFAs) were not detected. However, when the organism was grown ex vivo in serum, the Fatty Acid composition was radically different with SCUFAs, which increase membrane fluidity, making up a substantial proportion of the total ( 37%) and BCFAs (>36%) making up the rest. Staphyloxanthin, an additional major membrane lipid component unique to S. aureus, tended to be greater in content in cells with high BCFAs or SCUFAs. Cells with high staphyloxanthin content had a lower membrane fluidity that was attributed to increased production of staphyloxanthin. S. aureus saves energy and carbon by utilizing host Fatty Acids for part of its total Fatty Acids when growing in serum. The Fatty Acid composition of in vitro grown S. aureus is likely to be a poor reflection of the Fatty Acid composition and biophysical properties of the membrane when the organism is growing in an infection in view of the role of SCUFAs in staphylococcal membrane composition and virulence.