The Experts below are selected from a list of 186 Experts worldwide ranked by ideXlab platform

E W Mccleskey - One of the best experts on this subject based on the ideXlab platform.

  • cutaneous sensory neurons expressing the mrgprd receptor sense extracellular atp and are putative nociceptors
    Journal of Neurophysiology, 2008
    Co-Authors: G Dussor, M J Zylka, David J Anderson, E W Mccleskey
    Abstract:

    Sensory neurons expressing the Mrgprd receptor are known to innervate the outermost living layer of the epidermis, the Stratum Granulosum. The sensory modality that these neurons signal and the sti...

  • cutaneous sensory neurons expressing the mrgprd receptor sense extracellular atp and are putative nociceptors
    Journal of Neurophysiology, 2008
    Co-Authors: G Dussor, M J Zylka, David J Anderson, E W Mccleskey
    Abstract:

    Sensory neurons expressing the Mrgprd receptor are known to innervate the outermost living layer of the epidermis, the Stratum Granulosum. The sensory modality that these neurons signal and the stimulus that they respond to are not established, although immunocytochemical data suggest they could be nonpeptidergic nociceptors. Using patch clamp of dissociated mouse dorsal root ganglion (DRG) neurons, the present study demonstrates that Mrgprd+ neurons have several properties typical of nociceptors: long-duration action potentials, TTX-resistant Na+ current, and Ca2+ currents that are inhibited by mu opioids. Remarkably, Mrgprd+ neurons respond almost exclusively to extracellular ATP with currents similar to homomeric P2X3 receptors. They show little or no sensitivity to other putative nociceptive agonists, including capsaicin, cinnamaldehyde, menthol, pH 6.0, or glutamate. These properties, together with selective innervation of the Stratum Granulosum, indicate that Mrgprd+ neurons are nociceptors in the outer epidermis and may respond indirectly to external stimuli by detecting ATP release in the skin.

G Dussor - One of the best experts on this subject based on the ideXlab platform.

  • cutaneous sensory neurons expressing the mrgprd receptor sense extracellular atp and are putative nociceptors
    Journal of Neurophysiology, 2008
    Co-Authors: G Dussor, M J Zylka, David J Anderson, E W Mccleskey
    Abstract:

    Sensory neurons expressing the Mrgprd receptor are known to innervate the outermost living layer of the epidermis, the Stratum Granulosum. The sensory modality that these neurons signal and the sti...

  • cutaneous sensory neurons expressing the mrgprd receptor sense extracellular atp and are putative nociceptors
    Journal of Neurophysiology, 2008
    Co-Authors: G Dussor, M J Zylka, David J Anderson, E W Mccleskey
    Abstract:

    Sensory neurons expressing the Mrgprd receptor are known to innervate the outermost living layer of the epidermis, the Stratum Granulosum. The sensory modality that these neurons signal and the stimulus that they respond to are not established, although immunocytochemical data suggest they could be nonpeptidergic nociceptors. Using patch clamp of dissociated mouse dorsal root ganglion (DRG) neurons, the present study demonstrates that Mrgprd+ neurons have several properties typical of nociceptors: long-duration action potentials, TTX-resistant Na+ current, and Ca2+ currents that are inhibited by mu opioids. Remarkably, Mrgprd+ neurons respond almost exclusively to extracellular ATP with currents similar to homomeric P2X3 receptors. They show little or no sensitivity to other putative nociceptive agonists, including capsaicin, cinnamaldehyde, menthol, pH 6.0, or glutamate. These properties, together with selective innervation of the Stratum Granulosum, indicate that Mrgprd+ neurons are nociceptors in the outer epidermis and may respond indirectly to external stimuli by detecting ATP release in the skin.

Nicholas L. Simmons - One of the best experts on this subject based on the ideXlab platform.

  • expression and localization of monocarboxylate transporters and sodium proton exchangers in bovine rumen epithelium
    American Journal of Physiology-regulatory Integrative and Comparative Physiology, 2007
    Co-Authors: Chris Graham, Ian Gatherar, Iain S. Haslam, Michael Glanville, Nicholas L. Simmons
    Abstract:

    Monocarboxylate-H+ cotransporters, such as monocarboxylate transporter (MCT) SLC16A, have been suggested to mediate transruminal fluxes of short-chain fatty acids, ketone bodies, and lactate. Using an RT-PCR approach, we demonstrate expression of MCT1 (SLC16A1) and MCT2 (SLC16A7) mRNA in isolated bovine rumen epithelium. cDNA sequence from these PCR products combined with overlapping expressed sequence tag data allowed compilation of the complete open reading frames for MCT1 and MCT2. Immunohistochemical localization of MCT1 shows plasma membrane staining in cells of the Stratum basale, with intense staining of the basal aspects of the cells. Immunostaining decreased in the cell layers toward the rumen lumen, with weak staining in the Stratum spinsoum. Immunostaining in the Stratum Granulosum and Stratum corneum was essentially negative. Since monocarboxylate transport will load the cytosol with acid, expression and location of Na+/H+ exchanger (NHE) family members within the rumen epithelium were determined. RT-PCR demonstrates expression of multiple NHE family members, including NHE1, NHE2, NHE3, and NHE8. In contrast to MCT1, immunostaining showed that NHE1 was predominantly localized to the Stratum Granulosum, with a progressive decrease toward the Stratum basale. NHE2 immunostaining was observed mainly at an intracellular location in the Stratum basale, Stratum spinosum, and Stratum Granulosum. Given the anatomic localization of MCT1, NHE1, and NHE2, the mechanism of transruminal short-chain fatty acid, ketone body, and lactate transfer is discussed in relation to a functional model of the rumen epithelium comprising an apical permeability barrier at the Stratum Granulosum, with a cell syncitium linking the Stratum Granulosum to the blood-facing Stratum basale.

  • Expression and localization of monocarboxylate transporters and sodium/proton exchangers in bovine rumen epithelium
    American Journal of Physiology-regulatory Integrative and Comparative Physiology, 2006
    Co-Authors: Chris Graham, Ian Gatherar, Iain S. Haslam, Michael Glanville, Nicholas L. Simmons
    Abstract:

    Monocarboxylate-H+ cotransporters, such as monocarboxylate transporter (MCT) SLC16A, have been suggested to mediate transruminal fluxes of short-chain fatty acids, ketone bodies, and lactate. Using an RT-PCR approach, we demonstrate expression of MCT1 (SLC16A1) and MCT2 (SLC16A7) mRNA in isolated bovine rumen epithelium. cDNA sequence from these PCR products combined with overlapping expressed sequence tag data allowed compilation of the complete open reading frames for MCT1 and MCT2. Immunohistochemical localization of MCT1 shows plasma membrane staining in cells of the Stratum basale, with intense staining of the basal aspects of the cells. Immunostaining decreased in the cell layers toward the rumen lumen, with weak staining in the Stratum spinsoum. Immunostaining in the Stratum Granulosum and Stratum corneum was essentially negative. Since monocarboxylate transport will load the cytosol with acid, expression and location of Na+/H+ exchanger (NHE) family members within the rumen epithelium were determined. RT-PCR demonstrates expression of multiple NHE family members, including NHE1, NHE2, NHE3, and NHE8. In contrast to MCT1, immunostaining showed that NHE1 was predominantly localized to the Stratum Granulosum, with a progressive decrease toward the Stratum basale. NHE2 immunostaining was observed mainly at an intracellular location in the Stratum basale, Stratum spinosum, and Stratum Granulosum. Given the anatomic localization of MCT1, NHE1, and NHE2, the mechanism of transruminal short-chain fatty acid, ketone body, and lactate transfer is discussed in relation to a functional model of the rumen epithelium comprising an apical permeability barrier at the Stratum Granulosum, with a cell syncitium linking the Stratum Granulosum to the blood-facing Stratum basale.

M J Zylka - One of the best experts on this subject based on the ideXlab platform.

  • cutaneous sensory neurons expressing the mrgprd receptor sense extracellular atp and are putative nociceptors
    Journal of Neurophysiology, 2008
    Co-Authors: G Dussor, M J Zylka, David J Anderson, E W Mccleskey
    Abstract:

    Sensory neurons expressing the Mrgprd receptor are known to innervate the outermost living layer of the epidermis, the Stratum Granulosum. The sensory modality that these neurons signal and the sti...

  • cutaneous sensory neurons expressing the mrgprd receptor sense extracellular atp and are putative nociceptors
    Journal of Neurophysiology, 2008
    Co-Authors: G Dussor, M J Zylka, David J Anderson, E W Mccleskey
    Abstract:

    Sensory neurons expressing the Mrgprd receptor are known to innervate the outermost living layer of the epidermis, the Stratum Granulosum. The sensory modality that these neurons signal and the stimulus that they respond to are not established, although immunocytochemical data suggest they could be nonpeptidergic nociceptors. Using patch clamp of dissociated mouse dorsal root ganglion (DRG) neurons, the present study demonstrates that Mrgprd+ neurons have several properties typical of nociceptors: long-duration action potentials, TTX-resistant Na+ current, and Ca2+ currents that are inhibited by mu opioids. Remarkably, Mrgprd+ neurons respond almost exclusively to extracellular ATP with currents similar to homomeric P2X3 receptors. They show little or no sensitivity to other putative nociceptive agonists, including capsaicin, cinnamaldehyde, menthol, pH 6.0, or glutamate. These properties, together with selective innervation of the Stratum Granulosum, indicate that Mrgprd+ neurons are nociceptors in the outer epidermis and may respond indirectly to external stimuli by detecting ATP release in the skin.

David J Anderson - One of the best experts on this subject based on the ideXlab platform.

  • cutaneous sensory neurons expressing the mrgprd receptor sense extracellular atp and are putative nociceptors
    Journal of Neurophysiology, 2008
    Co-Authors: G Dussor, M J Zylka, David J Anderson, E W Mccleskey
    Abstract:

    Sensory neurons expressing the Mrgprd receptor are known to innervate the outermost living layer of the epidermis, the Stratum Granulosum. The sensory modality that these neurons signal and the sti...

  • cutaneous sensory neurons expressing the mrgprd receptor sense extracellular atp and are putative nociceptors
    Journal of Neurophysiology, 2008
    Co-Authors: G Dussor, M J Zylka, David J Anderson, E W Mccleskey
    Abstract:

    Sensory neurons expressing the Mrgprd receptor are known to innervate the outermost living layer of the epidermis, the Stratum Granulosum. The sensory modality that these neurons signal and the stimulus that they respond to are not established, although immunocytochemical data suggest they could be nonpeptidergic nociceptors. Using patch clamp of dissociated mouse dorsal root ganglion (DRG) neurons, the present study demonstrates that Mrgprd+ neurons have several properties typical of nociceptors: long-duration action potentials, TTX-resistant Na+ current, and Ca2+ currents that are inhibited by mu opioids. Remarkably, Mrgprd+ neurons respond almost exclusively to extracellular ATP with currents similar to homomeric P2X3 receptors. They show little or no sensitivity to other putative nociceptive agonists, including capsaicin, cinnamaldehyde, menthol, pH 6.0, or glutamate. These properties, together with selective innervation of the Stratum Granulosum, indicate that Mrgprd+ neurons are nociceptors in the outer epidermis and may respond indirectly to external stimuli by detecting ATP release in the skin.