The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Ophir D Klein - One of the best experts on this subject based on the ideXlab platform.
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perp regulates enamel formation via effects on cell cell adhesion and gene expression
Journal of Cell Science, 2011Co-Authors: Andrew H Jheon, Laura D Attardi, Rebecca A Ihrie, Pasha Mostowfi, Tiziano Pramparo, Eli D. Sone, Malcolm L Snead, Ophir D KleinAbstract:Little is known about the role of cell–cell adhesion in the development of mineralized tissues. Here we report that PERP, a tetraspan membrane protein essential for epithelial integrity, regulates enamel formation. PERP is necessary for proper cell attachment and gene expression during tooth development, and its expression is controlled by P63, a master regulator of stratified epithelial development. During enamel formation, PERP is localized to the interface between the enamel-producing ameloblasts and the Stratum Intermedium (SI), a layer of cells subjacent to the ameloblasts. Perp-null mice display dramatic enamel defects, which are caused, in part, by the detachment of ameloblasts from the SI. Microarray analysis comparing gene expression in teeth of wild-type and Perp-null mice identified several differentially expressed genes during enamel formation. Analysis of these genes in ameloblast-derived LS8 cells upon knockdown of PERP confirmed the role for PERP in the regulation of gene expression. Together, our data show that PERP is necessary for the integrity of the ameloblast–SI interface and that a lack of Perp causes downregulation of genes that are required for proper enamel formation.
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PERP regulates enamel formation via effects on cell–cell adhesion and gene expression
Journal of Cell Science, 2011Co-Authors: Andrew H Jheon, Laura D Attardi, Rebecca A Ihrie, Pasha Mostowfi, Tiziano Pramparo, Eli D. Sone, Malcolm L Snead, Ophir D KleinAbstract:Little is known about the role of cell–cell adhesion in the development of mineralized tissues. Here we report that PERP, a tetraspan membrane protein essential for epithelial integrity, regulates enamel formation. PERP is necessary for proper cell attachment and gene expression during tooth development, and its expression is controlled by P63, a master regulator of stratified epithelial development. During enamel formation, PERP is localized to the interface between the enamel-producing ameloblasts and the Stratum Intermedium (SI), a layer of cells subjacent to the ameloblasts. Perp-null mice display dramatic enamel defects, which are caused, in part, by the detachment of ameloblasts from the SI. Microarray analysis comparing gene expression in teeth of wild-type and Perp-null mice identified several differentially expressed genes during enamel formation. Analysis of these genes in ameloblast-derived LS8 cells upon knockdown of PERP confirmed the role for PERP in the regulation of gene expression. Together, our data show that PERP is necessary for the integrity of the ameloblast–SI interface and that a lack of Perp causes downregulation of genes that are required for proper enamel formation.
Andrew H Jheon - One of the best experts on this subject based on the ideXlab platform.
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perp regulates enamel formation via effects on cell cell adhesion and gene expression
Journal of Cell Science, 2011Co-Authors: Andrew H Jheon, Laura D Attardi, Rebecca A Ihrie, Pasha Mostowfi, Tiziano Pramparo, Eli D. Sone, Malcolm L Snead, Ophir D KleinAbstract:Little is known about the role of cell–cell adhesion in the development of mineralized tissues. Here we report that PERP, a tetraspan membrane protein essential for epithelial integrity, regulates enamel formation. PERP is necessary for proper cell attachment and gene expression during tooth development, and its expression is controlled by P63, a master regulator of stratified epithelial development. During enamel formation, PERP is localized to the interface between the enamel-producing ameloblasts and the Stratum Intermedium (SI), a layer of cells subjacent to the ameloblasts. Perp-null mice display dramatic enamel defects, which are caused, in part, by the detachment of ameloblasts from the SI. Microarray analysis comparing gene expression in teeth of wild-type and Perp-null mice identified several differentially expressed genes during enamel formation. Analysis of these genes in ameloblast-derived LS8 cells upon knockdown of PERP confirmed the role for PERP in the regulation of gene expression. Together, our data show that PERP is necessary for the integrity of the ameloblast–SI interface and that a lack of Perp causes downregulation of genes that are required for proper enamel formation.
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PERP regulates enamel formation via effects on cell–cell adhesion and gene expression
Journal of Cell Science, 2011Co-Authors: Andrew H Jheon, Laura D Attardi, Rebecca A Ihrie, Pasha Mostowfi, Tiziano Pramparo, Eli D. Sone, Malcolm L Snead, Ophir D KleinAbstract:Little is known about the role of cell–cell adhesion in the development of mineralized tissues. Here we report that PERP, a tetraspan membrane protein essential for epithelial integrity, regulates enamel formation. PERP is necessary for proper cell attachment and gene expression during tooth development, and its expression is controlled by P63, a master regulator of stratified epithelial development. During enamel formation, PERP is localized to the interface between the enamel-producing ameloblasts and the Stratum Intermedium (SI), a layer of cells subjacent to the ameloblasts. Perp-null mice display dramatic enamel defects, which are caused, in part, by the detachment of ameloblasts from the SI. Microarray analysis comparing gene expression in teeth of wild-type and Perp-null mice identified several differentially expressed genes during enamel formation. Analysis of these genes in ameloblast-derived LS8 cells upon knockdown of PERP confirmed the role for PERP in the regulation of gene expression. Together, our data show that PERP is necessary for the integrity of the ameloblast–SI interface and that a lack of Perp causes downregulation of genes that are required for proper enamel formation.
Thomas G H Diekwisch - One of the best experts on this subject based on the ideXlab platform.
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daughters of the enamel organ development fate and function of the Stratum Intermedium stellate reticulum and outer enamel epithelium
Stem Cells and Development, 2016Co-Authors: Hui Liu, Xiulin Yan, Mirali Pandya, Xianghong Luan, Thomas G H DiekwischAbstract:The tooth enamel organ (EO) is a complex epithelial cell assembly involved in multiple aspects of tooth development, including amelogenesis. The present study focuses on the role of the nonameloblast layers of the EO, the Stratum Intermedium, the stellate reticulum, and the outer enamel epithelium (OEE). The secretory stage Stratum Intermedium was distinguished by p63-positive epithelial stem cell marks, highly specific alkaline phosphatase labeling, as well as multiple desmosomes and gap junctions. At the location of the presecretory stage stellate reticulum, the pre-eruption EO prominently featured the papillary layer (PL) as a keratin immunopositive network of epithelial strands between tooth crowns and oral epithelium. PL cell strands contained numerous p63-positive epithelial stem cells, while BrdU proliferative cells were detected at the outer boundaries of the PL, suggesting that the stellate reticulum/PL epithelial cell sheath proliferated to facilitate an epithelial seal during tooth eruption. Comparative histology studies demonstrated continuity between the OEE and the general lamina of continuous tooth replacement in reptiles, and the outer layer of Hertwig's epithelial root sheath in humans, implicating the OEE as the formative layer for continuous tooth replacement and tooth root extension. Cell fate studies in organ culture verified that the cervical portion of the mouse molar EO gave rise to Malassez rest-like cell islands. Together, these studies indicate that the nonameloblast layers of the EO play multiple roles during odontogenesis, including the maintenance of several p63-positive stem cell reservoirs, a role during tooth root morphogenesis and tooth succession, a stabilizing function for the ameloblast layer, the facilitation of ion transport from the EO capillaries to the enamel layer, as well as safe and seamless tooth eruption.
Poels, Lambert G. - One of the best experts on this subject based on the ideXlab platform.
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Scheme of tooth development (human)
2026Co-Authors: Poels, Lambert G.Abstract:A. Survey of tooth germ (bell stage, embryo) magnification x 35 objective. B. Formation of enamel and dentin (bell stage, embryo) magnification x 350 objective. 1. pulp organ; 2. odontoblasts; 3. mantle predentin; 4. dentin; 5. enamel; 6. initial enamel; 7. inner dental epithelium (ameloblasts); 8. Stratum Intermedium; 9. stellate reticulum; 10. outer dental epithelium with mesenchyme; 11. dental lamina; 12. primordium of permanent tooth; 13. non-keratinized squamous epithelium of oral cavity; 14. alveolar bone (mandible
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Late cap stage in tooth development - human, embryo
2026Co-Authors: Poels, Lambert G.Abstract:Stain: Azan. From top to bottom: Top side stellate reticulum (enamel pulp) consisting of a network of ectoderm-derived cells; Right side outer dental epithelium with part of the fibrous tooth follicle. This epithelium will further develop downwards as the outer layer of the Hertwig's epithelial root sheath; Darker stained cell layers of the Stratum Intermedium close to the stellate reticulum; Columnar inner dental epithelium (presecretory ameloblasts) at the distal side (secretion area) delimits by basement membrane from the dental papilla; Network of mesenchyme-derived cells of the dental papilla (future pulp) is more condensed close to the basement membrane
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Late cap stage of tooth development - human, embryo; low magnification
2026Co-Authors: Poels, Lambert G.Abstract:Stain: Azan. From top to bottom: Stratified ectoderm with a distinct basal layer (red line) of cuboid cells; Dental lamina giving rise to the cap stage (center) and to the primordium of permanent tooth (right); Odontogenic organ or enamel organ (future deciduous tooth surrounded by fibrous tooth follicle); Outer dental epithelium encloses the stellate reticulum consisting of a network of ectoderm-derived cells; Inner dental epithelium is seen as a dark red zone of future ameloblasts covered by a few cell layers of the Stratum Intermedium; Future odontoblasts are localized just below the translucent zone close to the ameloblasts; Dental papilla extends outside the cervical loop; it consists of a compact network of fibroblast-like cells (so-called pulpal cells); Blood vessels of the papilla and tooth follicle are present; At the bottom alveolar bone formation (dark blue)
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Ameloblasts and odontoblasts in tooth development - advanced bell stage, human, embryo
2026Co-Authors: Poels, Lambert G.Abstract:Stain: Azan. From top to bottom: Stellate reticulum consisting of a loose network of ectoderm-derived cells; Cell layers of the Stratum Intermedium; Columnar presecretory ameloblasts with their nuclear area close to the Stratum Intermedium, and at the distal side (secretion area) oriented towards predentin (blue); Predentin with fan-like arrangement of Korff's fibers (deep blue); Columnar odontoblasts in a epithelioid arrangement with their secretion area close to the predentin (blue); Mesenchym-derived fibroblast-like cells (so-called pulpal cells) producing collagen (thin blue threads) of the dental papilla (future pulp)
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Predentin formation at the cuspal tip in tooth development - bell stage, human, embryo
2026Co-Authors: Poels, Lambert G.Abstract:Stain: Azan. From top to bottom: Stellate reticulum consisting of a network of ectoderm-derived cells; Cell layers of the Stratum Intermedium; Columnar (presecretory) ameloblasts with their upper side (nuclear area) in close contact with the Stratum Intermedium, and at the distal side (secretion area) oriented towards predentin (blue); Odontoblasts in a epithelioid arrangement with their secretion area close to the predentin (blue); In close contact with the odontoblasts a network of fibroblast-like cells (so-called pulpal cells) of the dental papilla (future pulp); blood vessels are also present
Malcolm L Snead - One of the best experts on this subject based on the ideXlab platform.
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Immunoperoxidase immunostaining of Slc26a1, Slc26a6 and Slc26a7 in secretory- and maturation-stage enamel organ.
2015Co-Authors: Kaifeng Yin, Malcolm L Snead, Rodrigo S. Lacruz, Shane N. White, Yuejuan Lei, Xin Wen, Manoocher Soleimani, Ira Kurtz, Michael L. PaineAbstract:Immunostaining procedures were applied to the sagittal sections prepared from paraffin-embedded 4-week-old rat mandibles. A. Slc26a1 in secretory-stage ameloblasts (S); B. Slc26a1 in smooth-ended ameloblasts at maturation stage (M-SA); C. Slc26a1 in ruffle-ended ameloblasts at maturation stage (M-RA); D. Slc26a6 in secretory-stage ameloblasts (S); E. Slc26a6 in smooth-ended ameloblasts at maturation stage (M-SA); F. Slc26a6 in ruffle-ended ameloblasts at maturation stage (M-RA); G. Slc26a7 in secretory-stage ameloblasts (S); H. Slc26a7 in smooth-ended ameloblasts at maturation stage (M-SA); I. Slc26a7 in ruffle-ended ameloblasts at maturation stage (M-RA); J-L. The sections that were incubated without antibodies served as negative controls for immunostaining. All images were collected under 20x magnification. Scale bar shown in Panel J (50μm). Slc26a1, Slc26a6 and Slc26a7 all showed expression on the apical membrane and/or within subapical cytoplasmic region (double black arrows). Positive staining in other regions was indicated by double black asterisks. SI—Stratum Intermedium; Am—Ameloblast; ES—Enamel space; CT—Connective tissue; PL—Papillary layer.
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Histological analysis.
2013Co-Authors: Rodrigo S. Lacruz, Malcolm L Snead, Yohei Nakayama, James Holcroft, Van Nguyen, Eszter Somogyi-ganss, Shane N. White, Michael L. Paine, Bernhard GanssAbstract:Sagittal sections of mandibular incisors of wild type (WT) and transgenic (tg57) mice were stained with hematoxylin and eosin. Comparable anatomical regions from apical (late secretory stage; a, b) and incisal (maturation stage; c, d) portions along the apical/incisal axis are shown and magnified in inserts. At the late secretory stage, the eosinophilic enamel matrix appears completely disorganized and thinner in tg57 animals, compared to WT, where an organized, striated pattern is visible. At the maturation stage (c, d), tg57 animals display a thinner enamel space containing more residual organic matrix compared to WT animals. The orientation that applies to all sections is indicated in (b). Abbreviations: ab-ameloblast layer; d-dentin; em-enamel matrix; es-enamel space; p-pulp; pl-papillary layer; rl-reticular layer; si-Stratum Intermedium; sr-stellate reticulum. Scale bar in (d): 200 µm.
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perp regulates enamel formation via effects on cell cell adhesion and gene expression
Journal of Cell Science, 2011Co-Authors: Andrew H Jheon, Laura D Attardi, Rebecca A Ihrie, Pasha Mostowfi, Tiziano Pramparo, Eli D. Sone, Malcolm L Snead, Ophir D KleinAbstract:Little is known about the role of cell–cell adhesion in the development of mineralized tissues. Here we report that PERP, a tetraspan membrane protein essential for epithelial integrity, regulates enamel formation. PERP is necessary for proper cell attachment and gene expression during tooth development, and its expression is controlled by P63, a master regulator of stratified epithelial development. During enamel formation, PERP is localized to the interface between the enamel-producing ameloblasts and the Stratum Intermedium (SI), a layer of cells subjacent to the ameloblasts. Perp-null mice display dramatic enamel defects, which are caused, in part, by the detachment of ameloblasts from the SI. Microarray analysis comparing gene expression in teeth of wild-type and Perp-null mice identified several differentially expressed genes during enamel formation. Analysis of these genes in ameloblast-derived LS8 cells upon knockdown of PERP confirmed the role for PERP in the regulation of gene expression. Together, our data show that PERP is necessary for the integrity of the ameloblast–SI interface and that a lack of Perp causes downregulation of genes that are required for proper enamel formation.
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PERP regulates enamel formation via effects on cell–cell adhesion and gene expression
Journal of Cell Science, 2011Co-Authors: Andrew H Jheon, Laura D Attardi, Rebecca A Ihrie, Pasha Mostowfi, Tiziano Pramparo, Eli D. Sone, Malcolm L Snead, Ophir D KleinAbstract:Little is known about the role of cell–cell adhesion in the development of mineralized tissues. Here we report that PERP, a tetraspan membrane protein essential for epithelial integrity, regulates enamel formation. PERP is necessary for proper cell attachment and gene expression during tooth development, and its expression is controlled by P63, a master regulator of stratified epithelial development. During enamel formation, PERP is localized to the interface between the enamel-producing ameloblasts and the Stratum Intermedium (SI), a layer of cells subjacent to the ameloblasts. Perp-null mice display dramatic enamel defects, which are caused, in part, by the detachment of ameloblasts from the SI. Microarray analysis comparing gene expression in teeth of wild-type and Perp-null mice identified several differentially expressed genes during enamel formation. Analysis of these genes in ameloblast-derived LS8 cells upon knockdown of PERP confirmed the role for PERP in the regulation of gene expression. Together, our data show that PERP is necessary for the integrity of the ameloblast–SI interface and that a lack of Perp causes downregulation of genes that are required for proper enamel formation.