The Experts below are selected from a list of 2235 Experts worldwide ranked by ideXlab platform
Chien-ming Shih - One of the best experts on this subject based on the ideXlab platform.
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First Detection and Molecular IdentifiCation of Borrelia garinii Spirochete from Ixodes ovatus Tick Ectoparasitized on Stray Cat in Taiwan
PLOS ONE, 2014Co-Authors: Li-lian Chao, Tsung-yu Ho, Chien-ming ShihAbstract:Borrelia garinii spirochete was detected for the first time in Ixodes ovatus tick ectoparasitized on Stray Cat in Taiwan. The genetic identity of this detected spirochete was determined by analyzing the gene sequence amplified by genospecies-specific polymerase chain reaction assays based on the 5S–23S intergenic spacer amplicon (rrf-rrl) and outer surface protein A (ospA) genes of B. burgdorferi sensu lato. Phylogenetic relationships were analyzed by comparing the sequences of rrf-rrl and ospA genes obtained from 27 strains of Borrelia spirochetes representing six genospecies of Borrelia. Seven major clades can be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony method. Phylogenetic analysis based on rrf-rrl gene revealed that this detected spirochete (strain IO-TP-TW) was genetically affiliated to the same clade with a high homogeneous sequences (96.7 to 98.1% similarity) within the genospecies of B. garinii and can be discriminated from other genospecies of Borrelia spirochetes. Interspecies analysis based on the genetic distance values indiCates a lower level ( 0.113) to the strains identified in I. ovatus collected from Japan and China. Intraspecies analysis also reveals a higher homogeneity (GD
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first detection and molecular identifiCation of borrelia garinii spirochete from ixodes ovatus tick ectoparasitized on Stray Cat in taiwan
PLOS ONE, 2014Co-Authors: Li-lian Chao, Tsung-yu Ho, Chien-ming ShihAbstract:Borrelia garinii spirochete was detected for the first time in Ixodes ovatus tick ectoparasitized on Stray Cat in Taiwan. The genetic identity of this detected spirochete was determined by analyzing the gene sequence amplified by genospecies-specific polymerase chain reaction assays based on the 5S–23S intergenic spacer amplicon (rrf-rrl) and outer surface protein A (ospA) genes of B. burgdorferi sensu lato. Phylogenetic relationships were analyzed by comparing the sequences of rrf-rrl and ospA genes obtained from 27 strains of Borrelia spirochetes representing six genospecies of Borrelia. Seven major clades can be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony method. Phylogenetic analysis based on rrf-rrl gene revealed that this detected spirochete (strain IO-TP-TW) was genetically affiliated to the same clade with a high homogeneous sequences (96.7 to 98.1% similarity) within the genospecies of B. garinii and can be discriminated from other genospecies of Borrelia spirochetes. Interspecies analysis based on the genetic distance values indiCates a lower level ( 0.113) to the strains identified in I. ovatus collected from Japan and China. Intraspecies analysis also reveals a higher homogeneity (GD<0.005) between tick (strain IO-TP-TW) and human (strain Bg-PP-TW1) isolates of B. garinii in Taiwan. This study provides the first evidence of B. garinii isolated and identified in an I. ovatus tick in Asia, and the higher homogeneity of B. garinii between tick and human strain may imply the risk of human infection by I. ovatus bite.
Mark J. Farnworth - One of the best experts on this subject based on the ideXlab platform.
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mapping the Stray domestic Cat felis Catus population in new zealand species distribution modelling with a climate change scenario and impliCations for protected areas
Applied Geography, 2015Co-Authors: Glenn Aguilar, Mark J. Farnworth, Linton WinderAbstract:Species distribution models of Stray Cats were developed using two types of occurrence data: (i) a combined dataset of Stray Cats and Cat colonies in Auckland and projected to the wider New Zealand area; and (ii) population density as an analogue for country-wide Stray Cat occurrence. These occurrence data, together with sets of environmental variables were used as input to the Maxent modelling tool to produce maps of suitability for the species. Environmental variables used in the models consist of current bioclimatic conditions, and a future climate scenario (RCP8.5 for year 2070 CCSM model). Commonly occurring bias in the modelling process due to latitude, the area for selecting background points in model evaluation, inherent spatial autocorrelation of occurrence points, and correlated bioclimatic variables were explicitly addressed. Results show that the North Island consistently provide more suitable areas for Stray Cats with increased suitability in a high emission climate change condition. Key protected areas at risk from the increased suitability to Stray Cats are also presented.
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Stray Cats in Auckland, New Zealand: Discovering geographic information for exploratory spatial analysis
Applied Geography, 2012Co-Authors: Glenn Aguilar, Mark J. FarnworthAbstract:Abstract Stray Cats are a common feature of urban landscapes and are associated with issues of animal welfare and negative environmental impacts. Management, planning and decision-making require readily accessible information on Stray Cats. However, much of the existing data is not immediately useful for a geographic information system (GIS) in terms of format, content and explicit loCation information. Spreadsheets we obtained from a single large shelter in the Auckland region. They contained records of Stray Cat pickups and admissions for an entire year ( n = 8573) of which 56.4% ( n = 4834) contained data that could be processed to derive relevant spatial information. The resulting data consisted of identified roads and areas of Auckland where the Stray Cats were found. Published census databases and shapefiles were matched with the data to build a GIS of Stray Cats. Global and local regression analysis was employed to discover spatial distribution characteristics including the identifiCation of areas with relatively high and low concentrations of Stray Cats and to explore relationships between socioeconomic condition and Stray Cat density. Significant clustering is more evident in South Auckland than elsewhere in the region. Specific geographical information is valuable, not only for understanding population dynamics of Stray Cats, but also to allow spatial and temporal targeting of resources to minimise their impact and promote responsible ownership.
Li-lian Chao - One of the best experts on this subject based on the ideXlab platform.
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First Detection and Molecular IdentifiCation of Borrelia garinii Spirochete from Ixodes ovatus Tick Ectoparasitized on Stray Cat in Taiwan
PLOS ONE, 2014Co-Authors: Li-lian Chao, Tsung-yu Ho, Chien-ming ShihAbstract:Borrelia garinii spirochete was detected for the first time in Ixodes ovatus tick ectoparasitized on Stray Cat in Taiwan. The genetic identity of this detected spirochete was determined by analyzing the gene sequence amplified by genospecies-specific polymerase chain reaction assays based on the 5S–23S intergenic spacer amplicon (rrf-rrl) and outer surface protein A (ospA) genes of B. burgdorferi sensu lato. Phylogenetic relationships were analyzed by comparing the sequences of rrf-rrl and ospA genes obtained from 27 strains of Borrelia spirochetes representing six genospecies of Borrelia. Seven major clades can be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony method. Phylogenetic analysis based on rrf-rrl gene revealed that this detected spirochete (strain IO-TP-TW) was genetically affiliated to the same clade with a high homogeneous sequences (96.7 to 98.1% similarity) within the genospecies of B. garinii and can be discriminated from other genospecies of Borrelia spirochetes. Interspecies analysis based on the genetic distance values indiCates a lower level ( 0.113) to the strains identified in I. ovatus collected from Japan and China. Intraspecies analysis also reveals a higher homogeneity (GD
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first detection and molecular identifiCation of borrelia garinii spirochete from ixodes ovatus tick ectoparasitized on Stray Cat in taiwan
PLOS ONE, 2014Co-Authors: Li-lian Chao, Tsung-yu Ho, Chien-ming ShihAbstract:Borrelia garinii spirochete was detected for the first time in Ixodes ovatus tick ectoparasitized on Stray Cat in Taiwan. The genetic identity of this detected spirochete was determined by analyzing the gene sequence amplified by genospecies-specific polymerase chain reaction assays based on the 5S–23S intergenic spacer amplicon (rrf-rrl) and outer surface protein A (ospA) genes of B. burgdorferi sensu lato. Phylogenetic relationships were analyzed by comparing the sequences of rrf-rrl and ospA genes obtained from 27 strains of Borrelia spirochetes representing six genospecies of Borrelia. Seven major clades can be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony method. Phylogenetic analysis based on rrf-rrl gene revealed that this detected spirochete (strain IO-TP-TW) was genetically affiliated to the same clade with a high homogeneous sequences (96.7 to 98.1% similarity) within the genospecies of B. garinii and can be discriminated from other genospecies of Borrelia spirochetes. Interspecies analysis based on the genetic distance values indiCates a lower level ( 0.113) to the strains identified in I. ovatus collected from Japan and China. Intraspecies analysis also reveals a higher homogeneity (GD<0.005) between tick (strain IO-TP-TW) and human (strain Bg-PP-TW1) isolates of B. garinii in Taiwan. This study provides the first evidence of B. garinii isolated and identified in an I. ovatus tick in Asia, and the higher homogeneity of B. garinii between tick and human strain may imply the risk of human infection by I. ovatus bite.
Glenn Aguilar - One of the best experts on this subject based on the ideXlab platform.
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mapping the Stray domestic Cat felis Catus population in new zealand species distribution modelling with a climate change scenario and impliCations for protected areas
Applied Geography, 2015Co-Authors: Glenn Aguilar, Mark J. Farnworth, Linton WinderAbstract:Species distribution models of Stray Cats were developed using two types of occurrence data: (i) a combined dataset of Stray Cats and Cat colonies in Auckland and projected to the wider New Zealand area; and (ii) population density as an analogue for country-wide Stray Cat occurrence. These occurrence data, together with sets of environmental variables were used as input to the Maxent modelling tool to produce maps of suitability for the species. Environmental variables used in the models consist of current bioclimatic conditions, and a future climate scenario (RCP8.5 for year 2070 CCSM model). Commonly occurring bias in the modelling process due to latitude, the area for selecting background points in model evaluation, inherent spatial autocorrelation of occurrence points, and correlated bioclimatic variables were explicitly addressed. Results show that the North Island consistently provide more suitable areas for Stray Cats with increased suitability in a high emission climate change condition. Key protected areas at risk from the increased suitability to Stray Cats are also presented.
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Stray Cats in Auckland, New Zealand: Discovering geographic information for exploratory spatial analysis
Applied Geography, 2012Co-Authors: Glenn Aguilar, Mark J. FarnworthAbstract:Abstract Stray Cats are a common feature of urban landscapes and are associated with issues of animal welfare and negative environmental impacts. Management, planning and decision-making require readily accessible information on Stray Cats. However, much of the existing data is not immediately useful for a geographic information system (GIS) in terms of format, content and explicit loCation information. Spreadsheets we obtained from a single large shelter in the Auckland region. They contained records of Stray Cat pickups and admissions for an entire year ( n = 8573) of which 56.4% ( n = 4834) contained data that could be processed to derive relevant spatial information. The resulting data consisted of identified roads and areas of Auckland where the Stray Cats were found. Published census databases and shapefiles were matched with the data to build a GIS of Stray Cats. Global and local regression analysis was employed to discover spatial distribution characteristics including the identifiCation of areas with relatively high and low concentrations of Stray Cats and to explore relationships between socioeconomic condition and Stray Cat density. Significant clustering is more evident in South Auckland than elsewhere in the region. Specific geographical information is valuable, not only for understanding population dynamics of Stray Cats, but also to allow spatial and temporal targeting of resources to minimise their impact and promote responsible ownership.
Tsung-yu Ho - One of the best experts on this subject based on the ideXlab platform.
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First Detection and Molecular IdentifiCation of Borrelia garinii Spirochete from Ixodes ovatus Tick Ectoparasitized on Stray Cat in Taiwan
PLOS ONE, 2014Co-Authors: Li-lian Chao, Tsung-yu Ho, Chien-ming ShihAbstract:Borrelia garinii spirochete was detected for the first time in Ixodes ovatus tick ectoparasitized on Stray Cat in Taiwan. The genetic identity of this detected spirochete was determined by analyzing the gene sequence amplified by genospecies-specific polymerase chain reaction assays based on the 5S–23S intergenic spacer amplicon (rrf-rrl) and outer surface protein A (ospA) genes of B. burgdorferi sensu lato. Phylogenetic relationships were analyzed by comparing the sequences of rrf-rrl and ospA genes obtained from 27 strains of Borrelia spirochetes representing six genospecies of Borrelia. Seven major clades can be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony method. Phylogenetic analysis based on rrf-rrl gene revealed that this detected spirochete (strain IO-TP-TW) was genetically affiliated to the same clade with a high homogeneous sequences (96.7 to 98.1% similarity) within the genospecies of B. garinii and can be discriminated from other genospecies of Borrelia spirochetes. Interspecies analysis based on the genetic distance values indiCates a lower level ( 0.113) to the strains identified in I. ovatus collected from Japan and China. Intraspecies analysis also reveals a higher homogeneity (GD
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first detection and molecular identifiCation of borrelia garinii spirochete from ixodes ovatus tick ectoparasitized on Stray Cat in taiwan
PLOS ONE, 2014Co-Authors: Li-lian Chao, Tsung-yu Ho, Chien-ming ShihAbstract:Borrelia garinii spirochete was detected for the first time in Ixodes ovatus tick ectoparasitized on Stray Cat in Taiwan. The genetic identity of this detected spirochete was determined by analyzing the gene sequence amplified by genospecies-specific polymerase chain reaction assays based on the 5S–23S intergenic spacer amplicon (rrf-rrl) and outer surface protein A (ospA) genes of B. burgdorferi sensu lato. Phylogenetic relationships were analyzed by comparing the sequences of rrf-rrl and ospA genes obtained from 27 strains of Borrelia spirochetes representing six genospecies of Borrelia. Seven major clades can be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony method. Phylogenetic analysis based on rrf-rrl gene revealed that this detected spirochete (strain IO-TP-TW) was genetically affiliated to the same clade with a high homogeneous sequences (96.7 to 98.1% similarity) within the genospecies of B. garinii and can be discriminated from other genospecies of Borrelia spirochetes. Interspecies analysis based on the genetic distance values indiCates a lower level ( 0.113) to the strains identified in I. ovatus collected from Japan and China. Intraspecies analysis also reveals a higher homogeneity (GD<0.005) between tick (strain IO-TP-TW) and human (strain Bg-PP-TW1) isolates of B. garinii in Taiwan. This study provides the first evidence of B. garinii isolated and identified in an I. ovatus tick in Asia, and the higher homogeneity of B. garinii between tick and human strain may imply the risk of human infection by I. ovatus bite.
