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T K F Wang - One of the best experts on this subject based on the ideXlab platform.

Terence R. Whitehead - One of the best experts on this subject based on the ideXlab platform.

  • Streptococcus bovis meningitis in an infant
    Journal of Clinical Microbiology, 2000
    Co-Authors: Russell J Grant, Terence R. Whitehead
    Abstract:

    Streptococcus bovis is a nonenterococcal, group D Streptococcus which has been identified as a causative agent for serious human infections, including endocarditis, bacteremia, and septic arthritis. Several cases of adult S. bovis meningitis have been reported, usually in association with underlying disease. In the neonatal period, it is an uncommon agent of meningitis. We report, to our knowledge, the third documented case of neonatal S. bovis meningitis in the English language literature. As in the previous cases, this neonate showed no anatomical or congenital immunologic lesion which might be expected to predispose the patient to meningitis. Sequencing of the 16S ribosomal DNA gene was performed and a new PCR test was used to secure a more reliable identification of the strain.

  • Genetic transformation of the ruminal bacteria Butyrivibrio fibrisolvens and Streptococcus bovis by electroporation
    Letters in Applied Microbiology, 1992
    Co-Authors: Terence R. Whitehead
    Abstract:

    Electroporation methods for introduction of plasmid DNA into the ruminal bacteria Butyrivibrio fibrisolvens and Streptococcus bovis were developed. Electroporation of the strictly anaerobic B. fibrisolvens was carried out in an anaerobic glovebox with a buffer of 10% (v/v) glycerol and 1 mM MgCl2 in distilled water. Streptococcus bovis electroporation could be carried out aerobically with a buffer of 10% (v/v) glycerol in distilled water. The Escherichia coli/Bacillus subtilis shuttle vector pBS42 could be transformed into B. fibrisolvens strain H17c, selecting for chloramphenicol resistance. The Streptococcus sanguis/E. coli shuttle vector pVA838 could replicate and express erythromycin resistance in Strep. bovis. Both vectors were stable in each organism in the absence of antibiotic selection. While the efficiency was low (

Shukching Wong - One of the best experts on this subject based on the ideXlab platform.

James B. Russell - One of the best experts on this subject based on the ideXlab platform.

Hilário Cuquetto Mantovani - One of the best experts on this subject based on the ideXlab platform.

  • Chemical composition and nutrient degradability in elephant grass silage inoculated with Streptococcus bovis isolated from the rumen
    Anais Da Academia Brasileira De Ciencias, 2013
    Co-Authors: Daniele De Jesus Ferreira, Anderson De Moura Zanine, Rogério De Paula Lana, Marinaldo Divino Ribeiro, Guilherme Ribeiro Alves, Hilário Cuquetto Mantovani
    Abstract:

    The objective of the present study was to assess the chemical and bromatological composition and in situ degradability of elephant grass silages inoculated with Streptococcus bovis isolated from cattle rumen. A complete randomized design was used with four treatments and six replications: elephant grass silage, elephant grass silage inoculated with 10 6 CFU/g Streptococcus bovis JB1 strains; elephant grass silage inoculated with 10 6 CFU/g Streptococcus bovis HC5 strains; elephant grass silage inoculated with 10 6 CFU/g Enterococcus faecium with six replications each. The pH and ammoniacal nitrogen values were lower (P

  • The effect of carbon and nitrogen sources on bovicin HC5 production by Streptococcus bovis HC5.
    Journal of Applied Microbiology, 2009
    Co-Authors: A.a.t. De Carvalho, Hilário Cuquetto Mantovani, Aline Dias Paiva, M.r. De Melo
    Abstract:

    AIMS: To investigate the effect of media composition and agroindustrial residues on bovicin HC5 production by Streptococcus bovis HC5. METHODS AND RESULTS: Batch cultures of S. bovis HC5 were grown in basal medium containing different carbon and nitrogen sources. The activity of cell-free and cell-associated bovicin HC5 was determined in culture supernatants and acidic extracts obtained from cell pellets, respectively. Streptococcus bovis HC5 produced bovicin using a variety of carbon and nitrogen sources. The highest specific activity was obtained in media containing 16 g l(-1) of glucose, after 16 h of incubation. The peak in cell-free and cell-associated bovicin HC5 activity was detected when S. bovis HC5 cultures reached stationary phase. The bovicin HC5 specific activity and bacterial cell mass increased approximately 3-fold when yeast extract and trypticase (0.5 and 1.0 g l(-1), respectively) were added together to the basal medium. Streptococcus bovis HC5 cultures produced bovicin HC5 in cheese whey and sugar cane juice and maximal volumetric productivity was obtained after 12 h of incubation. CONCLUSIONS: Streptococcus bovis HC5 is a versatile lactic acid bacterium that can utilize several carbon and nitrogen sources for bovicin HC5 production. This bacterium could be a useful model to study bacteriocin production in the rumen ecosystem. SIGNIFICANCE AND IMPACT OF THE STUDY: The use of agroindustrial residues as carbon sources could have an economical impact on bovicin HC5 production. To our knowledge, this is the first report to show the use of sugar cane juice for bacteriocin production by lactic acid bacteria.

  • bovicin hc5 a lantibiotic produced by Streptococcus bovis hc5 catalyzes the efflux of intracellular potassium but not atp
    Antimicrobial Agents and Chemotherapy, 2008
    Co-Authors: Hilário Cuquetto Mantovani, James B. Russell
    Abstract:

    Bovicin HC5, a broad-spectrum lantibiotic produced by Streptococcus bovis HC5, catalyzed the efflux of intracellular potassium from Streptococcus bovis JB1, a sensitive strain. The level of ATP also decreased, but this decline appeared to be caused by the activity of the F 1 F 0 ATPase rather than efflux per se.