The Experts below are selected from a list of 42 Experts worldwide ranked by ideXlab platform
Zuzana Hurnikova - One of the best experts on this subject based on the ideXlab platform.
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Apoptosis as the adaptation mechanism in survival of Trichinella spiralis in the host
Parasitology Research, 2011Co-Authors: Pavel Babal, Rosica Milcheva, Svetlozara Petkova, Pavol Janega, Zuzana HurnikovaAbstract:The study evaluates the role of apoptosis-inducing factor (AIF) in the process of Striated Muscle Cell transformation after occupation by Trichinella spiralis . Its relationship with other apoptosis-related factors [apoptotic protease-activating factor 1, Bcl-2 associated protein X (BAX), Bcl-2, caspase 3, survivin, poly (ADP-ribose) polymerase-1 (PARP-1), and endothelial and inducible (iNOS) nitric oxide synthase] was evaluated by immunohistochemistry. In the context of low BAX and caspase 3 expression and strong distribution of AIF in the sarcoplasm and nucleus at the very early stage of infection, we suppose that AIF-mediated signaling is involved in the apoptosis activation in the area of Trichinella occupation. In the time course of nurse Cell formation, survivin and caspase 3 migrated into the enlarged nuclei with strong PARP-1 expression. In the end of encapsulation of Trichinella , expression of all proapoptotic factors ceased and only survivin in the nuclei and Bcl-2 positivity in the cytoplasm persisted in the formed nurse Cell. The expression of sarcoplasmic iNOS was absent during the process of Muscle Cell de-differentiation and reappeared within the nurse Cell. It seems that upregulation and downregulation of factors of apoptosis in the skeletal Muscle Cell represents an adaptive mechanism providing a comfortable niche for the parasite.
Pavel Babal - One of the best experts on this subject based on the ideXlab platform.
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Apoptosis as the adaptation mechanism in survival of Trichinella spiralis in the host
Parasitology Research, 2011Co-Authors: Pavel Babal, Rosica Milcheva, Svetlozara Petkova, Pavol Janega, Zuzana HurnikovaAbstract:The study evaluates the role of apoptosis-inducing factor (AIF) in the process of Striated Muscle Cell transformation after occupation by Trichinella spiralis . Its relationship with other apoptosis-related factors [apoptotic protease-activating factor 1, Bcl-2 associated protein X (BAX), Bcl-2, caspase 3, survivin, poly (ADP-ribose) polymerase-1 (PARP-1), and endothelial and inducible (iNOS) nitric oxide synthase] was evaluated by immunohistochemistry. In the context of low BAX and caspase 3 expression and strong distribution of AIF in the sarcoplasm and nucleus at the very early stage of infection, we suppose that AIF-mediated signaling is involved in the apoptosis activation in the area of Trichinella occupation. In the time course of nurse Cell formation, survivin and caspase 3 migrated into the enlarged nuclei with strong PARP-1 expression. In the end of encapsulation of Trichinella , expression of all proapoptotic factors ceased and only survivin in the nuclei and Bcl-2 positivity in the cytoplasm persisted in the formed nurse Cell. The expression of sarcoplasmic iNOS was absent during the process of Muscle Cell de-differentiation and reappeared within the nurse Cell. It seems that upregulation and downregulation of factors of apoptosis in the skeletal Muscle Cell represents an adaptive mechanism providing a comfortable niche for the parasite.
Takehiko Amano - One of the best experts on this subject based on the ideXlab platform.
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production and secretion of nerve growth factor by clonal Striated Muscle Cell line g8 1
Neurochemistry International, 1992Co-Authors: Toshifumi Yamamoto, Tohru Yamakuni, Noriko Okabe, Takehiko AmanoAbstract:Abstract Two specific methods, Northern blot analysis using a 50 nucleotides probe to the conserved region of the nerve growth factor (NGF) gene, and enzyme immunoassay using a monoclonal biotinylated rat anti-NGF IgG-avidin conjugated peroxidase system, were used to demonstrate the production and secretion of NGF by mouse Striated Muscle Cell line G8-1. Calcium ionophore, A23187 (0.1–1 μM), forskolin (0.1–100 μM) and dibutyryl cyclic AMP (0.1–10 mM) strongly decreased the secretion of ir-NGF. The level of NGF mRNA was decreased by veratridine, A23187, forskolin and cyclic AMP but not by cyclic GMP. Consequently, we conclude that the secretion of NGF molecules paralleled the changes of NGF mRNA levels in the Cells induced by all agents tested. Carmamylcholine also decreased the level of NGF mRNA. Immunoblot analysis suggested that denatured ir-NGF molecules exist in a higher molecular weight form (22 KDa) than those of mouse submaxillary gland (13 KDa). Both Ca 2+ - and cAMP mediated mechanisms contribute to the decreased production of NGF mRNA in the Cells and the consequent inhibition of secretion of NGF molecules. Finally, molecular cloning of NGF of G8-1 Cells was conducted and confirmed the structure of the gene that consists of 1, 3, and 4 exons deleting exon 2. Thus, G8-1 NGF is derived from transcript B.
Svetlozara Petkova - One of the best experts on this subject based on the ideXlab platform.
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Apoptosis as the adaptation mechanism in survival of Trichinella spiralis in the host
Parasitology Research, 2011Co-Authors: Pavel Babal, Rosica Milcheva, Svetlozara Petkova, Pavol Janega, Zuzana HurnikovaAbstract:The study evaluates the role of apoptosis-inducing factor (AIF) in the process of Striated Muscle Cell transformation after occupation by Trichinella spiralis . Its relationship with other apoptosis-related factors [apoptotic protease-activating factor 1, Bcl-2 associated protein X (BAX), Bcl-2, caspase 3, survivin, poly (ADP-ribose) polymerase-1 (PARP-1), and endothelial and inducible (iNOS) nitric oxide synthase] was evaluated by immunohistochemistry. In the context of low BAX and caspase 3 expression and strong distribution of AIF in the sarcoplasm and nucleus at the very early stage of infection, we suppose that AIF-mediated signaling is involved in the apoptosis activation in the area of Trichinella occupation. In the time course of nurse Cell formation, survivin and caspase 3 migrated into the enlarged nuclei with strong PARP-1 expression. In the end of encapsulation of Trichinella , expression of all proapoptotic factors ceased and only survivin in the nuclei and Bcl-2 positivity in the cytoplasm persisted in the formed nurse Cell. The expression of sarcoplasmic iNOS was absent during the process of Muscle Cell de-differentiation and reappeared within the nurse Cell. It seems that upregulation and downregulation of factors of apoptosis in the skeletal Muscle Cell represents an adaptive mechanism providing a comfortable niche for the parasite.
Pavol Janega - One of the best experts on this subject based on the ideXlab platform.
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Apoptosis as the adaptation mechanism in survival of Trichinella spiralis in the host
Parasitology Research, 2011Co-Authors: Pavel Babal, Rosica Milcheva, Svetlozara Petkova, Pavol Janega, Zuzana HurnikovaAbstract:The study evaluates the role of apoptosis-inducing factor (AIF) in the process of Striated Muscle Cell transformation after occupation by Trichinella spiralis . Its relationship with other apoptosis-related factors [apoptotic protease-activating factor 1, Bcl-2 associated protein X (BAX), Bcl-2, caspase 3, survivin, poly (ADP-ribose) polymerase-1 (PARP-1), and endothelial and inducible (iNOS) nitric oxide synthase] was evaluated by immunohistochemistry. In the context of low BAX and caspase 3 expression and strong distribution of AIF in the sarcoplasm and nucleus at the very early stage of infection, we suppose that AIF-mediated signaling is involved in the apoptosis activation in the area of Trichinella occupation. In the time course of nurse Cell formation, survivin and caspase 3 migrated into the enlarged nuclei with strong PARP-1 expression. In the end of encapsulation of Trichinella , expression of all proapoptotic factors ceased and only survivin in the nuclei and Bcl-2 positivity in the cytoplasm persisted in the formed nurse Cell. The expression of sarcoplasmic iNOS was absent during the process of Muscle Cell de-differentiation and reappeared within the nurse Cell. It seems that upregulation and downregulation of factors of apoptosis in the skeletal Muscle Cell represents an adaptive mechanism providing a comfortable niche for the parasite.