The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Holger Zimmermann - One of the best experts on this subject based on the ideXlab platform.
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integrin αvβ3 vitronectin receptor is a candidate receptor for the virulent echovirus 9 strain barty
Journal of General Virology, 1999Co-Authors: Birgit Nelsensalz, Hans J Eggers, Holger ZimmermannAbstract:The enterovirus echovirus 9 strain Barty (E9/Barty) is pathogenic for newborn mice as well as for humans. In contrast to the apathogenic prototype strain Hill, strain Barty encodes an RGD motif in the C-terminal part of the structural protein VP1. Data are presented that show that E9/Barty binds its target cells via contact of the RGD motif to the αvβ3 integrin (vitronectin receptor), whereas prototype Hill uses a different, still unidentified receptor site. Furthermore, virus titres of murine Muscle Tissue were compared after infection of newborn and 1-, 2-, 3- and 12-week-old mice. The replication capacity of the virus decreased dramatically with age of the infected mice. Since E9/Barty does not replicate or replicates only poorly in mice older than about 5 days, and expression of the vitronectin receptor is reported to be down-regulated in Striated Muscle Tissue during development, it is suggested that susceptibility of mice to this echovirus infection is controlled by the availability of αvβ3 integrin.
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Integrin alpha(v)beta3 (vitronectin receptor) is a candidate receptor for the virulent echovirus 9 strain Barty.
The Journal of general virology, 1999Co-Authors: Birgit Nelsen-salz, Hans J Eggers, Holger ZimmermannAbstract:The enterovirus echovirus 9 strain Barty (E9/Barty) is pathogenic for newborn mice as well as for humans. In contrast to the apathogenic prototype strain Hill, strain Barty encodes an RGD motif in the C-terminal part of the structural protein VP1. Data are presented that show that E9/Barty binds its target cells via contact of the RGD motif to the alpha(v)beta3 integrin (vitronectin receptor), whereas prototype Hill uses a different, still unidentified receptor site. Furthermore, virus titres of murine Muscle Tissue were compared after infection of newborn and 1-, 2-, 3- and 12-week-old mice. The replication capacity of the virus decreased dramatically with age of the infected mice. Since E9/Barty does not replicate or replicates only poorly in mice older than about 5 days, and expression of the vitronectin receptor is reported to be down-regulated in Striated Muscle Tissue during development, it is suggested that susceptibility of mice to this echovirus infection is controlled by the availability of alpha(v)beta3 integrin.
Hans J Eggers - One of the best experts on this subject based on the ideXlab platform.
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integrin αvβ3 vitronectin receptor is a candidate receptor for the virulent echovirus 9 strain barty
Journal of General Virology, 1999Co-Authors: Birgit Nelsensalz, Hans J Eggers, Holger ZimmermannAbstract:The enterovirus echovirus 9 strain Barty (E9/Barty) is pathogenic for newborn mice as well as for humans. In contrast to the apathogenic prototype strain Hill, strain Barty encodes an RGD motif in the C-terminal part of the structural protein VP1. Data are presented that show that E9/Barty binds its target cells via contact of the RGD motif to the αvβ3 integrin (vitronectin receptor), whereas prototype Hill uses a different, still unidentified receptor site. Furthermore, virus titres of murine Muscle Tissue were compared after infection of newborn and 1-, 2-, 3- and 12-week-old mice. The replication capacity of the virus decreased dramatically with age of the infected mice. Since E9/Barty does not replicate or replicates only poorly in mice older than about 5 days, and expression of the vitronectin receptor is reported to be down-regulated in Striated Muscle Tissue during development, it is suggested that susceptibility of mice to this echovirus infection is controlled by the availability of αvβ3 integrin.
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Integrin alpha(v)beta3 (vitronectin receptor) is a candidate receptor for the virulent echovirus 9 strain Barty.
The Journal of general virology, 1999Co-Authors: Birgit Nelsen-salz, Hans J Eggers, Holger ZimmermannAbstract:The enterovirus echovirus 9 strain Barty (E9/Barty) is pathogenic for newborn mice as well as for humans. In contrast to the apathogenic prototype strain Hill, strain Barty encodes an RGD motif in the C-terminal part of the structural protein VP1. Data are presented that show that E9/Barty binds its target cells via contact of the RGD motif to the alpha(v)beta3 integrin (vitronectin receptor), whereas prototype Hill uses a different, still unidentified receptor site. Furthermore, virus titres of murine Muscle Tissue were compared after infection of newborn and 1-, 2-, 3- and 12-week-old mice. The replication capacity of the virus decreased dramatically with age of the infected mice. Since E9/Barty does not replicate or replicates only poorly in mice older than about 5 days, and expression of the vitronectin receptor is reported to be down-regulated in Striated Muscle Tissue during development, it is suggested that susceptibility of mice to this echovirus infection is controlled by the availability of alpha(v)beta3 integrin.
Dario Coletti - One of the best experts on this subject based on the ideXlab platform.
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Biomaterials and Bioactive Molecules to Drive Differentiation in Striated Muscle Tissue Engineering - Biomaterials and bioactive molecules to drive differentiation in Striated Muscle Tissue engineering
Frontiers in physiology, 2015Co-Authors: Valentina Di Felice, Giancarlo Forte, Dario ColettiAbstract:The generation of engineered Tissues and organs has entered into the clinical practice in response to the chronic lack of organ donors. In particular, for the skeletal and cardiac Muscles the translational potential of Tissue engineering approaches has clearly been shown, even though the construction of these Tissues lags behind others given the hierarchical, highly organized architecture of Striated Muscles. Failure of the cardiac Tissue leads to cardiovascular diseases, which are the leading cause of death in the developed world (Di Felice et al., 2014). On the other hand, there are many clinical cases where the loss of skeletal Muscle due to a traumatic injury, an aggressive tumor, or prolonged denervation may be cured by the regeneration of the Muscle Tissue (Perniconi and Coletti, 2014).
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Biomaterials and bioactive molecules to drive differentiation in Striated Muscle Tissue engineering
Frontiers in Physiology, 2015Co-Authors: Valentina Di Felice, Giancarlo Forte, Dario ColettiAbstract:The generation of engineered Tissues and organs has entered into the clinical practice in response to the chronic lack of organ donors. In particular, for the skeletal and cardiac Muscles the translational potential of Tissue engineering approaches has clearly been shown, even though the construction of these Tissues lags behind others given the hierarchical, highly organized architecture of Striated Muscles. Failure of the cardiac Tissue leads to cardiovascular diseases, which are the leading cause of death in the developed world (Di Felice et al., 2014). On the other hand, there are many clinical cases where the loss of skeletal Muscle due to a traumatic injury, an aggressive tumor, or prolonged denervation may be cured by the regeneration of the Muscle Tissue (Perniconi and Coletti, 2014).
Volker Schmid - One of the best experts on this subject based on the ideXlab platform.
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Characterization and expression analysis of an ancestor-type Pax gene in the hydrozoan jellyfish Podocoryne carnea.
Mechanisms of Development, 2000Co-Authors: Hans Groger, Patrick Callaerts, Walter J. Gehring, Volker SchmidAbstract:We characterized a Pax gene from the hydrozoan Podocoryne carnea. It is most similar to cnidarian Pax-B genes and encodes a paired domain, a homeodomain and an octapeptide. Expression analysis demonstrates the presence of Pax-B transcripts in eggs, the ectoderm of the planula larva and in a few scattered cells in the apical polyp ectoderm. In developing and mature medusae, Pax-B is localized in particular endodermal cells, oriented toward the outside. Pax-B is not expressed in Muscle cells. However, if isolated Striated Muscle Tissue is activated for transdifferentiation, the gene is expressed within 1 h, before new cell types, such as smooth Muscle and nerve cells, have formed. The expression data indicate that Pax-B is involved in nerve cell differentiation.
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reversible inactivation of cell type specific regulatory and structural genes in migrating isolated Striated Muscle cells of jellyfish
Developmental Biology, 1999Co-Authors: Nathalie Yanze, Hans Groger, Peter Muller, Volker SchmidAbstract:We have investigated, by RT-PCR and in situ hybridization, expression of genes encoding regulatory and structural proteins in migrating mononucleated Striated Muscle cells of the medusa Podocoryne carnea. Expression of the three homeobox genes Otx, Cnox1-Pc, and Cnox3-Pc; a specific splice variant of the myosin heavy chain gene (Myo1); and a tropomyosin (Tpm2) is stable in isolated and cultured Striated Muscle Tissue. When grafted onto cell-free extracellular matrix (ECM), Muscle cells of the Tissue fragments leave their native ECM and migrate as a coherent Tissue onto a host ECM until a stretched cell monolayer is formed. Shortly after the first cells of the grafted isolate have made contact with the host ECM, Otx and Cnox1-Pc expression is completely turned off in all cells of the graft, including those still adhering to their native ECM. Myo1 message disappears with a delay while the expression level of Tpm2 is strongly reduced. However, expression of the homeobox gene Cnox3-Pc, a msh-like gene, and of the ubiquitously expressed elongation factor 1 α is not affected by the migration process. All genes are reexpressed after 12–24 h, once migration of the cells has ceased. Our results demonstrate that the first few migrating cells induce a change in gene expression which is rapidly communicated throughout the entire Tissue. Furthermore, we showed that commitment of Striated Muscle cells remains stable despite the transient inactivation of cell-type-specific regulatory and structural genes.
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Transdifferentiation of isolated Striated Muscle of jellyfishin vitro: the initiation process
Seminars in cell biology, 1995Co-Authors: Volker Schmid, Susanne Reber-müllerAbstract:Abstract Fragments of Striated Muscle Tissue of Anthomedusae can be isolated and cultured. Without further treatment the isolated Muscle fragments maintain the differentiated state. When treated with enzymes degrading the adhering extracellular matrix, drugs activating protein kinase C or substances destroying the actin cytoskeleton, dedifferentiation and DNA replication are initiated and transdifferentiation to several new cell types occurs. Initiation of DNA replication seems to be correlated with a disturbance of cell–ECM interactions. If Muscle fragments are combined with isolated ECMs, cell migration onto the grafted ECMs occurs and DNA-replication and transdifferentiation are initiated in those cells which adhere to both, the native and the grafted ECM. If, however, the cells can stretch into a monolayer and adhere entirely to either the native or the grafted ECM, DNA-replication is inhibited. Carbohydrate moieties seem to be involved in mediating these cell–substrate interactions.
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Life stage and Tissue-specific expression of the homeobox gene cnox1-Pc of the hydrozoan Podocoryne carnea.
Developmental biology, 1995Co-Authors: Birgit L. Aerne, Christine D. Baader, Volker SchmidAbstract:We have isolated from the hydrozoan Podocoryne carnea a genomic clone and the corresponding cDNA of a homeobox gene. This gene, called cnox1-Pc, is most closely related to the lab class of homeobox genes. In the life cycle of P. carnea, which involves an asexually reproducing, sedentary polyp and a sexual planktonic medusa stage, we found cnox1-Pc transcripts specifically expressed in the medusa stage. Expression studies on isolated medusa organs and Tissues indicate that the message is absent in the tentacles and the feeding and sexual organ of the medusa and present in the Striated Muscle cells. During medusa bud development, expression of cnox1-Pc can be correlated with the development of the Striated Muscle Tissue, a cell type only found in the medusa. The results suggest that cnox1-Pc is involved in pattern formation of the medusa of P. carnea and might have a regulatory role in the differentiation of Striated Muscle.
Birgit Nelsen-salz - One of the best experts on this subject based on the ideXlab platform.
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Integrin alpha(v)beta3 (vitronectin receptor) is a candidate receptor for the virulent echovirus 9 strain Barty.
The Journal of general virology, 1999Co-Authors: Birgit Nelsen-salz, Hans J Eggers, Holger ZimmermannAbstract:The enterovirus echovirus 9 strain Barty (E9/Barty) is pathogenic for newborn mice as well as for humans. In contrast to the apathogenic prototype strain Hill, strain Barty encodes an RGD motif in the C-terminal part of the structural protein VP1. Data are presented that show that E9/Barty binds its target cells via contact of the RGD motif to the alpha(v)beta3 integrin (vitronectin receptor), whereas prototype Hill uses a different, still unidentified receptor site. Furthermore, virus titres of murine Muscle Tissue were compared after infection of newborn and 1-, 2-, 3- and 12-week-old mice. The replication capacity of the virus decreased dramatically with age of the infected mice. Since E9/Barty does not replicate or replicates only poorly in mice older than about 5 days, and expression of the vitronectin receptor is reported to be down-regulated in Striated Muscle Tissue during development, it is suggested that susceptibility of mice to this echovirus infection is controlled by the availability of alpha(v)beta3 integrin.