The Experts below are selected from a list of 291 Experts worldwide ranked by ideXlab platform
Prabhakar Pradhan - One of the best experts on this subject based on the ideXlab platform.
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optical study of stress hormone induced nanoscale Structural Alteration in brain using partial wave spectroscopic microscopy
Journal of Biophotonics, 2019Co-Authors: Shiva Bhandari, Pradeep Kumar Shukla, Huda M Almabadi, Peeyush Sahay, Radhakrishna Rao, Prabhakar PradhanAbstract:Chronic stress affects nano to microscale structures of the brain cells/tissues due to suppression of neural growths and reconnections, hence the neuronal activities. This results in depression, memory loss and even death of the brain cells. Our recently developed novel optical technique, partial wave spectroscopic microscopy has nanoscale sensitivity, and hence, can detect nanoscale changes in brain tissues due to stress. In this study, we applied this technique to quantify the stress related Structural changes in the corticosterone-treated mouse model of stress. Our results show that brains from corticosterone-treated mice showed higher nanoscale Structural disorder in the hippocampal region as compared to the brain from normal (vehicle) mice. The increase in Structural Alteration correlates with the duration of the stress. We further quantified the relative changes and the spatial localization of these changes in this mouse model and found out that the maximum changes occurred nearly symmetrically in both regions of the hippocampus. The mRNA for stress-related genes, brain-derived neurotrophic factor and tyrosine kinase-coupled receptor were also significantly reduced in the hippocampus of corticosterone-treated mice compared to that in control mice. These results indicate that chronic corticosterone treatment induces nanoscale Structural Alterations in mouse brain that corresponds to changes in stress-related gene expression.
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Quantitative analysis of nanoscale intranuclear Structural Alterations in hippocampal cells in chronic alcoholism via transmission electron microscopy imaging
Physical biology, 2017Co-Authors: Peeyush Sahay, Pradeep Kumar Shukla, Huda M Almabadi, Radhakrishna Rao, Hemendra Ghimire, Vibha Tripathi, Samarendra K. Mohanty, Prabhakar PradhanAbstract:Chronic alcoholism is known to alter the morphology of the hippocampus, an important region of cognitive function in the brain. Therefore, to understand the effect of chronic alcoholism on hippocampal neural cells, we employed a mouse model of chronic alcoholism and quantified intranuclear nanoscale Structural Alterations in these cells. Transmission electron microscopy (TEM) images of hippocampal neurons were obtained, and the degree of Structural Alteration in terms of mass density fluctuation was determined using the light-localization properties of optical media generated from TEM imaging. The results, which were obtained at length scales ranging from ~30 to 200 nm, show that 10–12 week-old mice fed a Lieber–DeCarli liquid (alcoholic) diet had a higher degree of Structural Alteration than control mice fed a normal diet without alcohol. The degree of Structural Alteration became significantly distinguishable at a sample length of ~100 nm, which is the typical length scale of the building blocks of cells, such as DNA, RNA, proteins and lipids. Interestingly, different degrees of Structural Alteration at such length scales suggest possible Structural rearrangement of chromatin inside the nuclei in chronic alcoholism.
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Quantitative analysis of the nanoscale intra-nuclear Structural Alterations in hippocampal cells in chronic alcoholism via transmission electron microscopy study
arXiv: Biological Physics, 2015Co-Authors: Peeyush Sahay, Pradeep Kumar Shukla, Huda M Almabadi, Radhakrishna Rao, Hemendra Ghimire, Vibha Tripathi, Samarendra K. Mohanty, Prabhakar PradhanAbstract:Chronic alcoholism is known to alter morphology of hippocampal, an important region of cognitive function in the brain. We performed quantification of nanoscale Structural Alterations in nuclei of hippocampal neuron cells due to chronic alcoholism, in mice model. Transmission electron microscopy images of the neuron cells were obtained and the degrees of Structural Alteration, in terms of mass density fluctuations, were determined using the recently developed light localization analysis technique. The results, obtained at the length scales ranging from 33 to 195 nm, show that the 4-week alcohol fed mice have higher degree of Structural Alteration in comparison to the control mice. The degree of Structural Alterations starts becoming significantly distinguishable around 100 nm sample length, which is the typical length scale of the building blocks of cells, such as DNA, RNA, etc. Different degrees of Structural Alterations at such length scales suggest possible Structural rearrangement of chromatin inside the nuclei.
Peeyush Sahay - One of the best experts on this subject based on the ideXlab platform.
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optical study of stress hormone induced nanoscale Structural Alteration in brain using partial wave spectroscopic microscopy
Journal of Biophotonics, 2019Co-Authors: Shiva Bhandari, Pradeep Kumar Shukla, Huda M Almabadi, Peeyush Sahay, Radhakrishna Rao, Prabhakar PradhanAbstract:Chronic stress affects nano to microscale structures of the brain cells/tissues due to suppression of neural growths and reconnections, hence the neuronal activities. This results in depression, memory loss and even death of the brain cells. Our recently developed novel optical technique, partial wave spectroscopic microscopy has nanoscale sensitivity, and hence, can detect nanoscale changes in brain tissues due to stress. In this study, we applied this technique to quantify the stress related Structural changes in the corticosterone-treated mouse model of stress. Our results show that brains from corticosterone-treated mice showed higher nanoscale Structural disorder in the hippocampal region as compared to the brain from normal (vehicle) mice. The increase in Structural Alteration correlates with the duration of the stress. We further quantified the relative changes and the spatial localization of these changes in this mouse model and found out that the maximum changes occurred nearly symmetrically in both regions of the hippocampus. The mRNA for stress-related genes, brain-derived neurotrophic factor and tyrosine kinase-coupled receptor were also significantly reduced in the hippocampus of corticosterone-treated mice compared to that in control mice. These results indicate that chronic corticosterone treatment induces nanoscale Structural Alterations in mouse brain that corresponds to changes in stress-related gene expression.
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Quantitative analysis of nanoscale intranuclear Structural Alterations in hippocampal cells in chronic alcoholism via transmission electron microscopy imaging
Physical biology, 2017Co-Authors: Peeyush Sahay, Pradeep Kumar Shukla, Huda M Almabadi, Radhakrishna Rao, Hemendra Ghimire, Vibha Tripathi, Samarendra K. Mohanty, Prabhakar PradhanAbstract:Chronic alcoholism is known to alter the morphology of the hippocampus, an important region of cognitive function in the brain. Therefore, to understand the effect of chronic alcoholism on hippocampal neural cells, we employed a mouse model of chronic alcoholism and quantified intranuclear nanoscale Structural Alterations in these cells. Transmission electron microscopy (TEM) images of hippocampal neurons were obtained, and the degree of Structural Alteration in terms of mass density fluctuation was determined using the light-localization properties of optical media generated from TEM imaging. The results, which were obtained at length scales ranging from ~30 to 200 nm, show that 10–12 week-old mice fed a Lieber–DeCarli liquid (alcoholic) diet had a higher degree of Structural Alteration than control mice fed a normal diet without alcohol. The degree of Structural Alteration became significantly distinguishable at a sample length of ~100 nm, which is the typical length scale of the building blocks of cells, such as DNA, RNA, proteins and lipids. Interestingly, different degrees of Structural Alteration at such length scales suggest possible Structural rearrangement of chromatin inside the nuclei in chronic alcoholism.
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Quantitative analysis of the nanoscale intra-nuclear Structural Alterations in hippocampal cells in chronic alcoholism via transmission electron microscopy study
arXiv: Biological Physics, 2015Co-Authors: Peeyush Sahay, Pradeep Kumar Shukla, Huda M Almabadi, Radhakrishna Rao, Hemendra Ghimire, Vibha Tripathi, Samarendra K. Mohanty, Prabhakar PradhanAbstract:Chronic alcoholism is known to alter morphology of hippocampal, an important region of cognitive function in the brain. We performed quantification of nanoscale Structural Alterations in nuclei of hippocampal neuron cells due to chronic alcoholism, in mice model. Transmission electron microscopy images of the neuron cells were obtained and the degrees of Structural Alteration, in terms of mass density fluctuations, were determined using the recently developed light localization analysis technique. The results, obtained at the length scales ranging from 33 to 195 nm, show that the 4-week alcohol fed mice have higher degree of Structural Alteration in comparison to the control mice. The degree of Structural Alterations starts becoming significantly distinguishable around 100 nm sample length, which is the typical length scale of the building blocks of cells, such as DNA, RNA, etc. Different degrees of Structural Alterations at such length scales suggest possible Structural rearrangement of chromatin inside the nuclei.
Huda M Almabadi - One of the best experts on this subject based on the ideXlab platform.
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optical study of stress hormone induced nanoscale Structural Alteration in brain using partial wave spectroscopic microscopy
Journal of Biophotonics, 2019Co-Authors: Shiva Bhandari, Pradeep Kumar Shukla, Huda M Almabadi, Peeyush Sahay, Radhakrishna Rao, Prabhakar PradhanAbstract:Chronic stress affects nano to microscale structures of the brain cells/tissues due to suppression of neural growths and reconnections, hence the neuronal activities. This results in depression, memory loss and even death of the brain cells. Our recently developed novel optical technique, partial wave spectroscopic microscopy has nanoscale sensitivity, and hence, can detect nanoscale changes in brain tissues due to stress. In this study, we applied this technique to quantify the stress related Structural changes in the corticosterone-treated mouse model of stress. Our results show that brains from corticosterone-treated mice showed higher nanoscale Structural disorder in the hippocampal region as compared to the brain from normal (vehicle) mice. The increase in Structural Alteration correlates with the duration of the stress. We further quantified the relative changes and the spatial localization of these changes in this mouse model and found out that the maximum changes occurred nearly symmetrically in both regions of the hippocampus. The mRNA for stress-related genes, brain-derived neurotrophic factor and tyrosine kinase-coupled receptor were also significantly reduced in the hippocampus of corticosterone-treated mice compared to that in control mice. These results indicate that chronic corticosterone treatment induces nanoscale Structural Alterations in mouse brain that corresponds to changes in stress-related gene expression.
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Quantitative analysis of nanoscale intranuclear Structural Alterations in hippocampal cells in chronic alcoholism via transmission electron microscopy imaging
Physical biology, 2017Co-Authors: Peeyush Sahay, Pradeep Kumar Shukla, Huda M Almabadi, Radhakrishna Rao, Hemendra Ghimire, Vibha Tripathi, Samarendra K. Mohanty, Prabhakar PradhanAbstract:Chronic alcoholism is known to alter the morphology of the hippocampus, an important region of cognitive function in the brain. Therefore, to understand the effect of chronic alcoholism on hippocampal neural cells, we employed a mouse model of chronic alcoholism and quantified intranuclear nanoscale Structural Alterations in these cells. Transmission electron microscopy (TEM) images of hippocampal neurons were obtained, and the degree of Structural Alteration in terms of mass density fluctuation was determined using the light-localization properties of optical media generated from TEM imaging. The results, which were obtained at length scales ranging from ~30 to 200 nm, show that 10–12 week-old mice fed a Lieber–DeCarli liquid (alcoholic) diet had a higher degree of Structural Alteration than control mice fed a normal diet without alcohol. The degree of Structural Alteration became significantly distinguishable at a sample length of ~100 nm, which is the typical length scale of the building blocks of cells, such as DNA, RNA, proteins and lipids. Interestingly, different degrees of Structural Alteration at such length scales suggest possible Structural rearrangement of chromatin inside the nuclei in chronic alcoholism.
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Quantitative analysis of the nanoscale intra-nuclear Structural Alterations in hippocampal cells in chronic alcoholism via transmission electron microscopy study
arXiv: Biological Physics, 2015Co-Authors: Peeyush Sahay, Pradeep Kumar Shukla, Huda M Almabadi, Radhakrishna Rao, Hemendra Ghimire, Vibha Tripathi, Samarendra K. Mohanty, Prabhakar PradhanAbstract:Chronic alcoholism is known to alter morphology of hippocampal, an important region of cognitive function in the brain. We performed quantification of nanoscale Structural Alterations in nuclei of hippocampal neuron cells due to chronic alcoholism, in mice model. Transmission electron microscopy images of the neuron cells were obtained and the degrees of Structural Alteration, in terms of mass density fluctuations, were determined using the recently developed light localization analysis technique. The results, obtained at the length scales ranging from 33 to 195 nm, show that the 4-week alcohol fed mice have higher degree of Structural Alteration in comparison to the control mice. The degree of Structural Alterations starts becoming significantly distinguishable around 100 nm sample length, which is the typical length scale of the building blocks of cells, such as DNA, RNA, etc. Different degrees of Structural Alterations at such length scales suggest possible Structural rearrangement of chromatin inside the nuclei.
Radhakrishna Rao - One of the best experts on this subject based on the ideXlab platform.
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optical study of stress hormone induced nanoscale Structural Alteration in brain using partial wave spectroscopic microscopy
Journal of Biophotonics, 2019Co-Authors: Shiva Bhandari, Pradeep Kumar Shukla, Huda M Almabadi, Peeyush Sahay, Radhakrishna Rao, Prabhakar PradhanAbstract:Chronic stress affects nano to microscale structures of the brain cells/tissues due to suppression of neural growths and reconnections, hence the neuronal activities. This results in depression, memory loss and even death of the brain cells. Our recently developed novel optical technique, partial wave spectroscopic microscopy has nanoscale sensitivity, and hence, can detect nanoscale changes in brain tissues due to stress. In this study, we applied this technique to quantify the stress related Structural changes in the corticosterone-treated mouse model of stress. Our results show that brains from corticosterone-treated mice showed higher nanoscale Structural disorder in the hippocampal region as compared to the brain from normal (vehicle) mice. The increase in Structural Alteration correlates with the duration of the stress. We further quantified the relative changes and the spatial localization of these changes in this mouse model and found out that the maximum changes occurred nearly symmetrically in both regions of the hippocampus. The mRNA for stress-related genes, brain-derived neurotrophic factor and tyrosine kinase-coupled receptor were also significantly reduced in the hippocampus of corticosterone-treated mice compared to that in control mice. These results indicate that chronic corticosterone treatment induces nanoscale Structural Alterations in mouse brain that corresponds to changes in stress-related gene expression.
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Quantitative analysis of nanoscale intranuclear Structural Alterations in hippocampal cells in chronic alcoholism via transmission electron microscopy imaging
Physical biology, 2017Co-Authors: Peeyush Sahay, Pradeep Kumar Shukla, Huda M Almabadi, Radhakrishna Rao, Hemendra Ghimire, Vibha Tripathi, Samarendra K. Mohanty, Prabhakar PradhanAbstract:Chronic alcoholism is known to alter the morphology of the hippocampus, an important region of cognitive function in the brain. Therefore, to understand the effect of chronic alcoholism on hippocampal neural cells, we employed a mouse model of chronic alcoholism and quantified intranuclear nanoscale Structural Alterations in these cells. Transmission electron microscopy (TEM) images of hippocampal neurons were obtained, and the degree of Structural Alteration in terms of mass density fluctuation was determined using the light-localization properties of optical media generated from TEM imaging. The results, which were obtained at length scales ranging from ~30 to 200 nm, show that 10–12 week-old mice fed a Lieber–DeCarli liquid (alcoholic) diet had a higher degree of Structural Alteration than control mice fed a normal diet without alcohol. The degree of Structural Alteration became significantly distinguishable at a sample length of ~100 nm, which is the typical length scale of the building blocks of cells, such as DNA, RNA, proteins and lipids. Interestingly, different degrees of Structural Alteration at such length scales suggest possible Structural rearrangement of chromatin inside the nuclei in chronic alcoholism.
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Quantitative analysis of the nanoscale intra-nuclear Structural Alterations in hippocampal cells in chronic alcoholism via transmission electron microscopy study
arXiv: Biological Physics, 2015Co-Authors: Peeyush Sahay, Pradeep Kumar Shukla, Huda M Almabadi, Radhakrishna Rao, Hemendra Ghimire, Vibha Tripathi, Samarendra K. Mohanty, Prabhakar PradhanAbstract:Chronic alcoholism is known to alter morphology of hippocampal, an important region of cognitive function in the brain. We performed quantification of nanoscale Structural Alterations in nuclei of hippocampal neuron cells due to chronic alcoholism, in mice model. Transmission electron microscopy images of the neuron cells were obtained and the degrees of Structural Alteration, in terms of mass density fluctuations, were determined using the recently developed light localization analysis technique. The results, obtained at the length scales ranging from 33 to 195 nm, show that the 4-week alcohol fed mice have higher degree of Structural Alteration in comparison to the control mice. The degree of Structural Alterations starts becoming significantly distinguishable around 100 nm sample length, which is the typical length scale of the building blocks of cells, such as DNA, RNA, etc. Different degrees of Structural Alterations at such length scales suggest possible Structural rearrangement of chromatin inside the nuclei.
Pradeep Kumar Shukla - One of the best experts on this subject based on the ideXlab platform.
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optical study of stress hormone induced nanoscale Structural Alteration in brain using partial wave spectroscopic microscopy
Journal of Biophotonics, 2019Co-Authors: Shiva Bhandari, Pradeep Kumar Shukla, Huda M Almabadi, Peeyush Sahay, Radhakrishna Rao, Prabhakar PradhanAbstract:Chronic stress affects nano to microscale structures of the brain cells/tissues due to suppression of neural growths and reconnections, hence the neuronal activities. This results in depression, memory loss and even death of the brain cells. Our recently developed novel optical technique, partial wave spectroscopic microscopy has nanoscale sensitivity, and hence, can detect nanoscale changes in brain tissues due to stress. In this study, we applied this technique to quantify the stress related Structural changes in the corticosterone-treated mouse model of stress. Our results show that brains from corticosterone-treated mice showed higher nanoscale Structural disorder in the hippocampal region as compared to the brain from normal (vehicle) mice. The increase in Structural Alteration correlates with the duration of the stress. We further quantified the relative changes and the spatial localization of these changes in this mouse model and found out that the maximum changes occurred nearly symmetrically in both regions of the hippocampus. The mRNA for stress-related genes, brain-derived neurotrophic factor and tyrosine kinase-coupled receptor were also significantly reduced in the hippocampus of corticosterone-treated mice compared to that in control mice. These results indicate that chronic corticosterone treatment induces nanoscale Structural Alterations in mouse brain that corresponds to changes in stress-related gene expression.
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Quantitative analysis of nanoscale intranuclear Structural Alterations in hippocampal cells in chronic alcoholism via transmission electron microscopy imaging
Physical biology, 2017Co-Authors: Peeyush Sahay, Pradeep Kumar Shukla, Huda M Almabadi, Radhakrishna Rao, Hemendra Ghimire, Vibha Tripathi, Samarendra K. Mohanty, Prabhakar PradhanAbstract:Chronic alcoholism is known to alter the morphology of the hippocampus, an important region of cognitive function in the brain. Therefore, to understand the effect of chronic alcoholism on hippocampal neural cells, we employed a mouse model of chronic alcoholism and quantified intranuclear nanoscale Structural Alterations in these cells. Transmission electron microscopy (TEM) images of hippocampal neurons were obtained, and the degree of Structural Alteration in terms of mass density fluctuation was determined using the light-localization properties of optical media generated from TEM imaging. The results, which were obtained at length scales ranging from ~30 to 200 nm, show that 10–12 week-old mice fed a Lieber–DeCarli liquid (alcoholic) diet had a higher degree of Structural Alteration than control mice fed a normal diet without alcohol. The degree of Structural Alteration became significantly distinguishable at a sample length of ~100 nm, which is the typical length scale of the building blocks of cells, such as DNA, RNA, proteins and lipids. Interestingly, different degrees of Structural Alteration at such length scales suggest possible Structural rearrangement of chromatin inside the nuclei in chronic alcoholism.
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Quantitative analysis of the nanoscale intra-nuclear Structural Alterations in hippocampal cells in chronic alcoholism via transmission electron microscopy study
arXiv: Biological Physics, 2015Co-Authors: Peeyush Sahay, Pradeep Kumar Shukla, Huda M Almabadi, Radhakrishna Rao, Hemendra Ghimire, Vibha Tripathi, Samarendra K. Mohanty, Prabhakar PradhanAbstract:Chronic alcoholism is known to alter morphology of hippocampal, an important region of cognitive function in the brain. We performed quantification of nanoscale Structural Alterations in nuclei of hippocampal neuron cells due to chronic alcoholism, in mice model. Transmission electron microscopy images of the neuron cells were obtained and the degrees of Structural Alteration, in terms of mass density fluctuations, were determined using the recently developed light localization analysis technique. The results, obtained at the length scales ranging from 33 to 195 nm, show that the 4-week alcohol fed mice have higher degree of Structural Alteration in comparison to the control mice. The degree of Structural Alterations starts becoming significantly distinguishable around 100 nm sample length, which is the typical length scale of the building blocks of cells, such as DNA, RNA, etc. Different degrees of Structural Alterations at such length scales suggest possible Structural rearrangement of chromatin inside the nuclei.
