The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
P E Kolattukudy - One of the best experts on this subject based on the ideXlab platform.
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regulation of constitutively expressed and induced cutinase genes by different zinc finger transcription factors in fusarium solani f sp pisi nectria haematococca
Journal of Biological Chemistry, 2002Co-Authors: Tatiana Sirakova, William F Ettinger, Linda Rogers, P E KolattukudyAbstract:Cutin monomers, generated by the low levels of constitutively expressed cutinase, induce high levels of cutinase that can help pathogenic fungi to penetrate into the host through the cuticle whose major Structural Polymer is cutin. We cloned three highly homologous cutinase genes, cut1, cut2, and cut3, from Fusarium solani f. pisi (Nectria haematococca). Amino acid sequence deduced from the nucleotide sequence of cut1 and cut2/3 matched with that of the peptides from cutinase 1 and cutinase 2, respectively, isolated from F. solani pisi grown on cutin as the sole carbon source. Induction of beta-glucuronidase gene fused to the promoters of the cutinases integrated into F. solani pisi genome indicates that cut2 is constitutively expressed and induced under starvation, whereas cut1 is highly induced by cutin monomers. A palindrome binding protein (PBP) previously cloned binds only to palindrome 1 of cut1 promoter but not palindrome 1 of cut2/3 which contains two base substitutions. PBP is thought to interfere with the binding of CTF1 alpha, the transcription factor involved in induction, to cut1 promoter and thus keep cut1 gene repressed until induced by cutin monomers. Because PBP cannot bind palindrome 1 of cut2, this gene is not repressed. CTF1 alpha does not transactivate cut2 promoter. A new Cys(6)Zn(2) motif-containing transcription factor, CTF1 beta, that binds palindrome 2 was cloned and sequenced. In yeast, CTF1 beta transactivates cut2 promoter but not cut1 promoter unless its palindrome 1 is mutated, unlike CTF1 alpha which transactivates cut1. Thus, CTF1 beta is involved in the constitutive expression of cut2 that causes production of low levels of cutin monomers that strongly induce cut1 using CTF1 alpha as the transcription factor.
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Regulation of constitutively expressed and induced cutinase genes by different zinc finger transcription factors in Fusarium solani f. sp. pisi (Nectria haematococca).
The Journal of biological chemistry, 2001Co-Authors: Tatiana Sirakova, William F Ettinger, Linda Rogers, P E KolattukudyAbstract:Abstract Cutin monomers, generated by the low levels of constitutively expressed cutinase, induce high levels of cutinase that can help pathogenic fungi to penetrate into the host through the cuticle whose major Structural Polymer is cutin. We cloned three highly homologous cutinase genes, cut1, cut2, andcut3, from Fusarium solani f. pisi(Nectria haematococca). Amino acid sequence deduced from the nucleotide sequence of cut1 andcut2/3 matched with that of the peptides from cutinase 1 and cutinase 2, respectively, isolated from F. solani pisigrown on cutin as the sole carbon source. Induction of β-glucuronidase gene fused to the promoters of the cutinases integrated into F. solani pisi genome indicates thatcut2 is constitutively expressed and induced under starvation, whereas cut1 is highly induced by cutin monomers. A palindrome binding protein (PBP) previously cloned binds only to palindrome 1 of cut1 promoter but not palindrome 1 of cut2/3 which contains two base substitutions. PBP is thought to interfere with the binding of CTF1α, the transcription factor involved in induction, to cut1 promoter and thus keep cut1 gene repressed until induced by cutin monomers. Because PBP cannot bind palindrome 1 of cut2, this gene is not repressed. CTF1α does not transactivate cut2 promoter. A new Cys6Zn2 motif-containing transcription factor, CTF1β, that binds palindrome 2 was cloned and sequenced. In yeast, CTF1β transactivates cut2 promoter but not cut1 promoter unless its palindrome 1 is mutated, unlike CTF1α which transactivates cut1. Thus, CTF1β is involved in the constitutive expression of cut2 that causes production of low levels of cutin monomers that strongly inducecut1 using CTF1α as the transcription factor.
Tatiana Sirakova - One of the best experts on this subject based on the ideXlab platform.
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regulation of constitutively expressed and induced cutinase genes by different zinc finger transcription factors in fusarium solani f sp pisi nectria haematococca
Journal of Biological Chemistry, 2002Co-Authors: Tatiana Sirakova, William F Ettinger, Linda Rogers, P E KolattukudyAbstract:Cutin monomers, generated by the low levels of constitutively expressed cutinase, induce high levels of cutinase that can help pathogenic fungi to penetrate into the host through the cuticle whose major Structural Polymer is cutin. We cloned three highly homologous cutinase genes, cut1, cut2, and cut3, from Fusarium solani f. pisi (Nectria haematococca). Amino acid sequence deduced from the nucleotide sequence of cut1 and cut2/3 matched with that of the peptides from cutinase 1 and cutinase 2, respectively, isolated from F. solani pisi grown on cutin as the sole carbon source. Induction of beta-glucuronidase gene fused to the promoters of the cutinases integrated into F. solani pisi genome indicates that cut2 is constitutively expressed and induced under starvation, whereas cut1 is highly induced by cutin monomers. A palindrome binding protein (PBP) previously cloned binds only to palindrome 1 of cut1 promoter but not palindrome 1 of cut2/3 which contains two base substitutions. PBP is thought to interfere with the binding of CTF1 alpha, the transcription factor involved in induction, to cut1 promoter and thus keep cut1 gene repressed until induced by cutin monomers. Because PBP cannot bind palindrome 1 of cut2, this gene is not repressed. CTF1 alpha does not transactivate cut2 promoter. A new Cys(6)Zn(2) motif-containing transcription factor, CTF1 beta, that binds palindrome 2 was cloned and sequenced. In yeast, CTF1 beta transactivates cut2 promoter but not cut1 promoter unless its palindrome 1 is mutated, unlike CTF1 alpha which transactivates cut1. Thus, CTF1 beta is involved in the constitutive expression of cut2 that causes production of low levels of cutin monomers that strongly induce cut1 using CTF1 alpha as the transcription factor.
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Regulation of constitutively expressed and induced cutinase genes by different zinc finger transcription factors in Fusarium solani f. sp. pisi (Nectria haematococca).
The Journal of biological chemistry, 2001Co-Authors: Tatiana Sirakova, William F Ettinger, Linda Rogers, P E KolattukudyAbstract:Abstract Cutin monomers, generated by the low levels of constitutively expressed cutinase, induce high levels of cutinase that can help pathogenic fungi to penetrate into the host through the cuticle whose major Structural Polymer is cutin. We cloned three highly homologous cutinase genes, cut1, cut2, andcut3, from Fusarium solani f. pisi(Nectria haematococca). Amino acid sequence deduced from the nucleotide sequence of cut1 andcut2/3 matched with that of the peptides from cutinase 1 and cutinase 2, respectively, isolated from F. solani pisigrown on cutin as the sole carbon source. Induction of β-glucuronidase gene fused to the promoters of the cutinases integrated into F. solani pisi genome indicates thatcut2 is constitutively expressed and induced under starvation, whereas cut1 is highly induced by cutin monomers. A palindrome binding protein (PBP) previously cloned binds only to palindrome 1 of cut1 promoter but not palindrome 1 of cut2/3 which contains two base substitutions. PBP is thought to interfere with the binding of CTF1α, the transcription factor involved in induction, to cut1 promoter and thus keep cut1 gene repressed until induced by cutin monomers. Because PBP cannot bind palindrome 1 of cut2, this gene is not repressed. CTF1α does not transactivate cut2 promoter. A new Cys6Zn2 motif-containing transcription factor, CTF1β, that binds palindrome 2 was cloned and sequenced. In yeast, CTF1β transactivates cut2 promoter but not cut1 promoter unless its palindrome 1 is mutated, unlike CTF1α which transactivates cut1. Thus, CTF1β is involved in the constitutive expression of cut2 that causes production of low levels of cutin monomers that strongly inducecut1 using CTF1α as the transcription factor.
Linda Rogers - One of the best experts on this subject based on the ideXlab platform.
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regulation of constitutively expressed and induced cutinase genes by different zinc finger transcription factors in fusarium solani f sp pisi nectria haematococca
Journal of Biological Chemistry, 2002Co-Authors: Tatiana Sirakova, William F Ettinger, Linda Rogers, P E KolattukudyAbstract:Cutin monomers, generated by the low levels of constitutively expressed cutinase, induce high levels of cutinase that can help pathogenic fungi to penetrate into the host through the cuticle whose major Structural Polymer is cutin. We cloned three highly homologous cutinase genes, cut1, cut2, and cut3, from Fusarium solani f. pisi (Nectria haematococca). Amino acid sequence deduced from the nucleotide sequence of cut1 and cut2/3 matched with that of the peptides from cutinase 1 and cutinase 2, respectively, isolated from F. solani pisi grown on cutin as the sole carbon source. Induction of beta-glucuronidase gene fused to the promoters of the cutinases integrated into F. solani pisi genome indicates that cut2 is constitutively expressed and induced under starvation, whereas cut1 is highly induced by cutin monomers. A palindrome binding protein (PBP) previously cloned binds only to palindrome 1 of cut1 promoter but not palindrome 1 of cut2/3 which contains two base substitutions. PBP is thought to interfere with the binding of CTF1 alpha, the transcription factor involved in induction, to cut1 promoter and thus keep cut1 gene repressed until induced by cutin monomers. Because PBP cannot bind palindrome 1 of cut2, this gene is not repressed. CTF1 alpha does not transactivate cut2 promoter. A new Cys(6)Zn(2) motif-containing transcription factor, CTF1 beta, that binds palindrome 2 was cloned and sequenced. In yeast, CTF1 beta transactivates cut2 promoter but not cut1 promoter unless its palindrome 1 is mutated, unlike CTF1 alpha which transactivates cut1. Thus, CTF1 beta is involved in the constitutive expression of cut2 that causes production of low levels of cutin monomers that strongly induce cut1 using CTF1 alpha as the transcription factor.
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Regulation of constitutively expressed and induced cutinase genes by different zinc finger transcription factors in Fusarium solani f. sp. pisi (Nectria haematococca).
The Journal of biological chemistry, 2001Co-Authors: Tatiana Sirakova, William F Ettinger, Linda Rogers, P E KolattukudyAbstract:Abstract Cutin monomers, generated by the low levels of constitutively expressed cutinase, induce high levels of cutinase that can help pathogenic fungi to penetrate into the host through the cuticle whose major Structural Polymer is cutin. We cloned three highly homologous cutinase genes, cut1, cut2, andcut3, from Fusarium solani f. pisi(Nectria haematococca). Amino acid sequence deduced from the nucleotide sequence of cut1 andcut2/3 matched with that of the peptides from cutinase 1 and cutinase 2, respectively, isolated from F. solani pisigrown on cutin as the sole carbon source. Induction of β-glucuronidase gene fused to the promoters of the cutinases integrated into F. solani pisi genome indicates thatcut2 is constitutively expressed and induced under starvation, whereas cut1 is highly induced by cutin monomers. A palindrome binding protein (PBP) previously cloned binds only to palindrome 1 of cut1 promoter but not palindrome 1 of cut2/3 which contains two base substitutions. PBP is thought to interfere with the binding of CTF1α, the transcription factor involved in induction, to cut1 promoter and thus keep cut1 gene repressed until induced by cutin monomers. Because PBP cannot bind palindrome 1 of cut2, this gene is not repressed. CTF1α does not transactivate cut2 promoter. A new Cys6Zn2 motif-containing transcription factor, CTF1β, that binds palindrome 2 was cloned and sequenced. In yeast, CTF1β transactivates cut2 promoter but not cut1 promoter unless its palindrome 1 is mutated, unlike CTF1α which transactivates cut1. Thus, CTF1β is involved in the constitutive expression of cut2 that causes production of low levels of cutin monomers that strongly inducecut1 using CTF1α as the transcription factor.
William F Ettinger - One of the best experts on this subject based on the ideXlab platform.
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regulation of constitutively expressed and induced cutinase genes by different zinc finger transcription factors in fusarium solani f sp pisi nectria haematococca
Journal of Biological Chemistry, 2002Co-Authors: Tatiana Sirakova, William F Ettinger, Linda Rogers, P E KolattukudyAbstract:Cutin monomers, generated by the low levels of constitutively expressed cutinase, induce high levels of cutinase that can help pathogenic fungi to penetrate into the host through the cuticle whose major Structural Polymer is cutin. We cloned three highly homologous cutinase genes, cut1, cut2, and cut3, from Fusarium solani f. pisi (Nectria haematococca). Amino acid sequence deduced from the nucleotide sequence of cut1 and cut2/3 matched with that of the peptides from cutinase 1 and cutinase 2, respectively, isolated from F. solani pisi grown on cutin as the sole carbon source. Induction of beta-glucuronidase gene fused to the promoters of the cutinases integrated into F. solani pisi genome indicates that cut2 is constitutively expressed and induced under starvation, whereas cut1 is highly induced by cutin monomers. A palindrome binding protein (PBP) previously cloned binds only to palindrome 1 of cut1 promoter but not palindrome 1 of cut2/3 which contains two base substitutions. PBP is thought to interfere with the binding of CTF1 alpha, the transcription factor involved in induction, to cut1 promoter and thus keep cut1 gene repressed until induced by cutin monomers. Because PBP cannot bind palindrome 1 of cut2, this gene is not repressed. CTF1 alpha does not transactivate cut2 promoter. A new Cys(6)Zn(2) motif-containing transcription factor, CTF1 beta, that binds palindrome 2 was cloned and sequenced. In yeast, CTF1 beta transactivates cut2 promoter but not cut1 promoter unless its palindrome 1 is mutated, unlike CTF1 alpha which transactivates cut1. Thus, CTF1 beta is involved in the constitutive expression of cut2 that causes production of low levels of cutin monomers that strongly induce cut1 using CTF1 alpha as the transcription factor.
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Regulation of constitutively expressed and induced cutinase genes by different zinc finger transcription factors in Fusarium solani f. sp. pisi (Nectria haematococca).
The Journal of biological chemistry, 2001Co-Authors: Tatiana Sirakova, William F Ettinger, Linda Rogers, P E KolattukudyAbstract:Abstract Cutin monomers, generated by the low levels of constitutively expressed cutinase, induce high levels of cutinase that can help pathogenic fungi to penetrate into the host through the cuticle whose major Structural Polymer is cutin. We cloned three highly homologous cutinase genes, cut1, cut2, andcut3, from Fusarium solani f. pisi(Nectria haematococca). Amino acid sequence deduced from the nucleotide sequence of cut1 andcut2/3 matched with that of the peptides from cutinase 1 and cutinase 2, respectively, isolated from F. solani pisigrown on cutin as the sole carbon source. Induction of β-glucuronidase gene fused to the promoters of the cutinases integrated into F. solani pisi genome indicates thatcut2 is constitutively expressed and induced under starvation, whereas cut1 is highly induced by cutin monomers. A palindrome binding protein (PBP) previously cloned binds only to palindrome 1 of cut1 promoter but not palindrome 1 of cut2/3 which contains two base substitutions. PBP is thought to interfere with the binding of CTF1α, the transcription factor involved in induction, to cut1 promoter and thus keep cut1 gene repressed until induced by cutin monomers. Because PBP cannot bind palindrome 1 of cut2, this gene is not repressed. CTF1α does not transactivate cut2 promoter. A new Cys6Zn2 motif-containing transcription factor, CTF1β, that binds palindrome 2 was cloned and sequenced. In yeast, CTF1β transactivates cut2 promoter but not cut1 promoter unless its palindrome 1 is mutated, unlike CTF1α which transactivates cut1. Thus, CTF1β is involved in the constitutive expression of cut2 that causes production of low levels of cutin monomers that strongly inducecut1 using CTF1α as the transcription factor.
Scott R. White - One of the best experts on this subject based on the ideXlab platform.
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Fully Recyclable Metastable Polymers and Composites
Chemistry of Materials, 2018Co-Authors: Evan M. Lloyd, Adam M Feinberg, Edgar B Mejia, Edwin K. Zen, Mostafa Yourdkhani, Hector Lopez Hernandez, Nancy R Sottos, Jeffrey S. Moore, Scott R. WhiteAbstract:Fully-cycled dePolymerization and rePolymerization of a low ceiling temperature Polymer, cyclic poly(phthalaldehyde) (cPPA), yielding high performance Structural Polymer is demonstrated. The facile conditions for cPPA dePolymerization circumvent the extreme conditions required to break down and recycle traditional thermoplastics and thermosets. cPPA dePolymerizes in as little as 14 min at 120 °C, with concurrent evaporation and quantitative recovery of the monomer. Polymerization of the recovered monomer produces cPPA with molecular and mechanical properties identical to the original material. DePolymerization of cPPA is also demonstrated in the presence of various carbon fiber reinforcements. Continuous carbon fibers retain 100% of their moduli and tensile strength through multiple generations of recycling, while fully recycled cPPA/carbon nanofiber composites exhibit mechanical properties equivalent to the original composite and show no degradation with cycling.
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Restoration of Large Damage Volumes in Polymers
Science, 2014Co-Authors: Scott R. White, Nancy R Sottos, Jeffrey S. Moore, Brett P. Krull, W.a. Santa Cruz, Ryan C. R. GergelyAbstract:The regenerative power of tissues and organs in biology has no analog in synthetic materials. Although self-healing of microscopic defects has been demonstrated, the regrowth of material lost through catastrophic damage requires a regenerative-like approach. We demonstrate a vascular synthetic system that restores mechanical performance in response to large-scale damage. Gap-filling scaffolds are created through a two-stage Polymer chemistry that initially forms a shape-conforming dynamic gel but later Polymerizes to a solid Structural Polymer with robust mechanical properties. Through the control of reaction kinetics and vascular delivery rate, we filled impacted regions that exceed 35 mm in diameter within 20 min and restored mechanical function within 3 hours. After restoration of impact damage, 62% of the total absorbed energy was recovered in comparison with that in initial impact tests.
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Self-healing Structural composite materials
Composites Part A-applied Science and Manufacturing, 2003Co-Authors: Michael R. Kessler, Nancy R Sottos, Scott R. WhiteAbstract:A self-healing fiber-reinforced Structural Polymer matrix composite material is demonstrated. In the composite, a microencapsulated healing agent and a solid chemical catalyst are dispersed within the Polymer matrix phase. Healing is triggered by crack propagation through the microcapsules, which then release the healing agent into the crack plane. Subsequent exposure of the healing agent to the chemical catalyst initiates Polymerization and bonding of the crack faces. Self-healing (autonomic healing) is demonstrated on width-tapered double cantilever beam fracture specimens in which a mid-plane delamination is introduced and then allowed to heal. Autonomic healing at room temperature yields as much as 45% recovery of virgin interlaminar fracture toughness, while healing at 80 °C increases the recovery to over 80%. The in situ kinetics of healing in Structural composites is investigated in comparison to that of neat epoxy resin.
