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Bektas Tepe - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial and antioxidative activities of the essential oils and methanol extracts of salvia cryptantha montbret et aucher ex benth and salvia multicaulis vahl
Food Chemistry, 2004Co-Authors: Bektas Tepe, Gulhan Vardarunlu, Ferda Candan, Dimitra Daferera, Moschos G Polissiou, Erol Donmez, Mehmet Unlu, Atalay SokmenAbstract:Abstract The essential oils and methanolic extracts of Salvia cryptantha and Salvia multicaulis were examined for their potential antimicrobial and radical scavenging activities. No, or slight, activity was observed when the polar and non-polar Subfractions of the extracts were tested, whereas essential oils exhibited antimicrobial activity. The essential oils isolated from S. cryptantha and S. multicaulis were analysed by GC–MS and 53 and 47 constituents were identified, respectively. Antioxidant activities of the polar subfraction and the essential oil were examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical-scavenging and lipid peroxidation assays. The essential oils, in particular, and the non-polar Subfractions of methanol extracts, showed antioxidant activity. In conclusion, the results indicate that the oils of S. cryptantha and S. multicaulis have the capacity to scavenge free radicals and to inhibit the growth of pathogenic microorganisms. Therefore they could be suitable for using as antimicrobial and antioxidative agents in the food industry.
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antimicrobial and antioxidant activity of the essential oil and methanol extracts of thymus pectinatus fisch et mey var pectinatus lamiaceae
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Gulhan Vardarunlu, Ferda Candan, Atalay Sokmen, Dimitra Daferera, Moschos G Polissiou, Munevver Sokmen, Erol Donmez, Bektas TepeAbstract:The essential oil, obtained by using a Clevenger distillation apparatus, and water-soluble (polar) and water-insoluble (nonpolar) Subfractions of the methanol extract of Thymus pectinatus Fisch. et Mey. var. pectinatus were assayed for their antimicrobial and antioxidant properties. No (or slight) antimicrobial activity was observed when the Subfractions were tested, whereas the essential oil showed strong antimicrobial activity against all microorganisms tested. Antioxidant activities of the polar subfraction and the essential oil were evaluated using 2,2-diphenyl-1-picrylhydrazyl, hydroxyl radical, superoxide radical scavenging, and lipid peroxidation assays. The essential oil, in particular, and the polar subfraction of the methanol extract showed antioxidant activity. The essential oil was analyzed by GC/MS, and 24 compounds, representing 99.6% of the essential oil, were identified: thymol, gamma-terpinene, p-cymene, carvacrol, and borneol were the main components. An antimicrobial activity test carried out with fractions of the essential oil showed that the activity was mainly observed in those fractions containing thymol, in particular, and carvacrol. The activity was, therefore, attributed to the presence of these compounds. Other constituents of the essential oil, such as borneol, gamma-terpinene, and p-cymene, could be also taken into account for their possible synergistic or antagonistic effects. On the other hand, thymol and carvacrol were individually found to possess weaker antioxidant activity than the crude oil itself, indicating that other constituents of the essential oil may contribute to the antioxidant activity observed. In conclusion, the results presented here show that T. pectinatus essential oil could be considered as a natural antimicrobial and antioxidant source.
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antimicrobial and antioxidant activity of the essential oil and methanol extracts of thymus pectinatus fisch et mey var pectinatus lamiaceae
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Gulhan Vardarunlu, Ferda Candan, Atalay Sokmen, Dimitra Daferera, Moschos G Polissiou, Munevver Sokmen, Erol Donmez, Bektas TepeAbstract:The essential oil, obtained by using a Clevenger distillation apparatus, and water-soluble (polar) and water-insoluble (nonpolar) Subfractions of the methanol extract of Thymus pectinatus Fisch. et Mey. var. pectinatus were assayed for their antimicrobial and antioxidant properties. No (or slight) antimicrobial activity was observed when the Subfractions were tested, whereas the essential oil showed strong antimicrobial activity against all microorganisms tested. Antioxidant activities of the polar subfraction and the essential oil were evaluated using 2,2-diphenyl-1-picrylhydrazyl, hydroxyl radical, superoxide radical scavenging, and lipid peroxidation assays. The essential oil, in particular, and the polar subfraction of the methanol extract showed antioxidant activity. The essential oil was analyzed by GC/MS, and 24 compounds, representing 99.6% of the essential oil, were identified: thymol, γ-terpinene, p-cymene, carvacrol, and borneol were the main components. An antimicrobial activity test carried ...
Atalay Sokmen - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial and antioxidative activities of the essential oils and methanol extracts of salvia cryptantha montbret et aucher ex benth and salvia multicaulis vahl
Food Chemistry, 2004Co-Authors: Bektas Tepe, Gulhan Vardarunlu, Ferda Candan, Dimitra Daferera, Moschos G Polissiou, Erol Donmez, Mehmet Unlu, Atalay SokmenAbstract:Abstract The essential oils and methanolic extracts of Salvia cryptantha and Salvia multicaulis were examined for their potential antimicrobial and radical scavenging activities. No, or slight, activity was observed when the polar and non-polar Subfractions of the extracts were tested, whereas essential oils exhibited antimicrobial activity. The essential oils isolated from S. cryptantha and S. multicaulis were analysed by GC–MS and 53 and 47 constituents were identified, respectively. Antioxidant activities of the polar subfraction and the essential oil were examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical-scavenging and lipid peroxidation assays. The essential oils, in particular, and the non-polar Subfractions of methanol extracts, showed antioxidant activity. In conclusion, the results indicate that the oils of S. cryptantha and S. multicaulis have the capacity to scavenge free radicals and to inhibit the growth of pathogenic microorganisms. Therefore they could be suitable for using as antimicrobial and antioxidative agents in the food industry.
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in vitro antibacterial antifungal and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of satureja hortensis l
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Medine Gulluce, Atalay Sokmen, Dimitra Daferera, Moschos G Polissiou, Munevver Sokmen, Guleray Agar, Hakan Ozkan, N Kartal, Fikrettin SahinAbstract:The present study was designated to evaluate the antimicrobial and antioxidant activities of the essential oil, obtained by using a Clevenger distillation apparatus, water soluble (polar) and water insoluble (nonpolar) Subfractions of the methanol extracts from aerial parts of Satureja hortensis L. plants, and methanol extract from calli established from the seeds using Gamborg's B5 basal media supplemented with indole-3-butyric acid (1.0 ppm), 6-benzylaminopurine (N6-benzyladenine) (1.0 ppm), and sucrose (2.5%). The antimicrobial test results showed that the essential oil of S. hortensis had great potential antimicrobial activities against all 23 bacteria and 15 fungi and yeast species tested. In contrast, the methanol extract from callus cultures and water soluble subfraction of the methanol extract did not show antimicrobial activities, but the nonpolar subfraction had antibacterial activity against only five out of 23 bacterial species, which were Bacillus subtilis, Enterococcus fecalis, Pseudomonas a...
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in vitro antibacterial antifungal and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of satureja hortensis l
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Medine Gulluce, Atalay Sokmen, Dimitra Daferera, Moschos G Polissiou, Munevver Sokmen, Guleray Agar, Hakan Ozkan, N Kartal, Fikrettin SahinAbstract:The present study was designated to evaluate the antimicrobial and antioxidant activities of the essential oil, obtained by using a Clevenger distillation apparatus, water soluble (polar) and water insoluble (nonpolar) Subfractions of the methanol extracts from aerial parts of Satureja hortensis L. plants, and methanol extract from calli established from the seeds using Gamborg's B5 basal media supplemented with indole-3-butyric acid (1.0 ppm), 6-benzylaminopurine (N(6)-benzyladenine) (1.0 ppm), and sucrose (2.5%). The antimicrobial test results showed that the essential oil of S. hortensis had great potential antimicrobial activities against all 23 bacteria and 15 fungi and yeast species tested. In contrast, the methanol extract from callus cultures and water soluble subfraction of the methanol extract did not show antimicrobial activities, but the nonpolar subfraction had antibacterial activity against only five out of 23 bacterial species, which were Bacillus subtilis, Enterococcus fecalis, Pseudomonas aeruginosa, Salmonella enteritidis, and Streptococcus pyogenes. Antioxidant studies suggested that the polar Subfractions of the methanol extract of intact plant and methanol extract of callus cultures were able to reduce the stable free radical 2,2-diphenyl-1-picrylhydrazyl to the yellow-colored diphenylpicrylhydrazine. In this assay, the strongest effect was observed for the tissue culture extract, with an IC(50) value of 23.76 +/- 0.80 microgram/mL, which could be compared with the synthetic antioxidant agent butylated hydroxytoluene. On the other hand, linoleic acid oxidation was 95% inhibited in the presence of the essential oil while the inhibition was 90% with the chloroform subfraction of the intact plant. The chemical composition of a hydrodistilled essential oil of S. hortensis was analyzed by gas chromatography (GC)/flame ionization detection (FID) and a GC-mass spectrometry system. A total 22 constituents representing 99.9% of the essential oil were identified by GC-FID analaysis. Thymol (29.0%), carvacrol (26.5%), gamma-terpinene (22.6%), and p-cymene (9.3%) were the main components.
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antimicrobial and antioxidant activity of the essential oil and methanol extracts of thymus pectinatus fisch et mey var pectinatus lamiaceae
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Gulhan Vardarunlu, Ferda Candan, Atalay Sokmen, Dimitra Daferera, Moschos G Polissiou, Munevver Sokmen, Erol Donmez, Bektas TepeAbstract:The essential oil, obtained by using a Clevenger distillation apparatus, and water-soluble (polar) and water-insoluble (nonpolar) Subfractions of the methanol extract of Thymus pectinatus Fisch. et Mey. var. pectinatus were assayed for their antimicrobial and antioxidant properties. No (or slight) antimicrobial activity was observed when the Subfractions were tested, whereas the essential oil showed strong antimicrobial activity against all microorganisms tested. Antioxidant activities of the polar subfraction and the essential oil were evaluated using 2,2-diphenyl-1-picrylhydrazyl, hydroxyl radical, superoxide radical scavenging, and lipid peroxidation assays. The essential oil, in particular, and the polar subfraction of the methanol extract showed antioxidant activity. The essential oil was analyzed by GC/MS, and 24 compounds, representing 99.6% of the essential oil, were identified: thymol, gamma-terpinene, p-cymene, carvacrol, and borneol were the main components. An antimicrobial activity test carried out with fractions of the essential oil showed that the activity was mainly observed in those fractions containing thymol, in particular, and carvacrol. The activity was, therefore, attributed to the presence of these compounds. Other constituents of the essential oil, such as borneol, gamma-terpinene, and p-cymene, could be also taken into account for their possible synergistic or antagonistic effects. On the other hand, thymol and carvacrol were individually found to possess weaker antioxidant activity than the crude oil itself, indicating that other constituents of the essential oil may contribute to the antioxidant activity observed. In conclusion, the results presented here show that T. pectinatus essential oil could be considered as a natural antimicrobial and antioxidant source.
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antimicrobial and antioxidant activity of the essential oil and methanol extracts of thymus pectinatus fisch et mey var pectinatus lamiaceae
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Gulhan Vardarunlu, Ferda Candan, Atalay Sokmen, Dimitra Daferera, Moschos G Polissiou, Munevver Sokmen, Erol Donmez, Bektas TepeAbstract:The essential oil, obtained by using a Clevenger distillation apparatus, and water-soluble (polar) and water-insoluble (nonpolar) Subfractions of the methanol extract of Thymus pectinatus Fisch. et Mey. var. pectinatus were assayed for their antimicrobial and antioxidant properties. No (or slight) antimicrobial activity was observed when the Subfractions were tested, whereas the essential oil showed strong antimicrobial activity against all microorganisms tested. Antioxidant activities of the polar subfraction and the essential oil were evaluated using 2,2-diphenyl-1-picrylhydrazyl, hydroxyl radical, superoxide radical scavenging, and lipid peroxidation assays. The essential oil, in particular, and the polar subfraction of the methanol extract showed antioxidant activity. The essential oil was analyzed by GC/MS, and 24 compounds, representing 99.6% of the essential oil, were identified: thymol, γ-terpinene, p-cymene, carvacrol, and borneol were the main components. An antimicrobial activity test carried ...
Feng Zhou - One of the best experts on this subject based on the ideXlab platform.
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in vitro inhibitory effect on pancreatic lipase activity of Subfractions from ethanol extracts of fermented oats avena sativa l and synergistic effect of three phenolic acids
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Ou Wang, Mengqian Wang, Jianfeng He, Yong Wang, Di Zhang, Feng Zhou, Baoping JiAbstract:The purpose of the present work is to study the pancreatic lipase inhibitory effects of different Subfractions (n-hexane, ethyl acetate (EA), n-butanol, and water) from ethanol extracts of nonfermented and fungi-fermented oats and to delineate the interactions of three primary phenolic acids in the EA Subfractions. The EA subfraction showed the highest inhibitory effect on pancreatic lipase activity at 1.5 mg/mL compared to the other Subfractions, regardless of whether the oats were fermented. Meanwhile, both of the EA Subfractions of two fungi-fermented oats demonstrated more effective inhibitory activity than that of nonfermented oats. A positive correlation between the total phenolics content and inhibitory activity was found. The inhibitory ability of the EA subfraction from nonfermented or fermented oats also displayed a dose-dependent effect. The standards of caffeic, ferulic, and p-coumaric acids, mainly included in EA Subfractions of fermented oats, also displayed a dose-dependent inhibitory effec...
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in vitro inhibitory effect on pancreatic lipase activity of Subfractions from ethanol extracts of fermented oats avena sativa l and synergistic effect of three phenolic acids
Journal of Agricultural and Food Chemistry, 2012Co-Authors: Shengbao Cai, Ou Wang, Mengqian Wang, Yong Wang, Di Zhang, Feng ZhouAbstract:The purpose of the present work is to study the pancreatic lipase inhibitory effects of different Subfractions (n-hexane, ethyl acetate (EA), n-butanol, and water) from ethanol extracts of nonfermented and fungi-fermented oats and to delineate the interactions of three primary phenolic acids in the EA Subfractions. The EA subfraction showed the highest inhibitory effect on pancreatic lipase activity at 1.5 mg/mL compared to the other Subfractions, regardless of whether the oats were fermented. Meanwhile, both of the EA Subfractions of two fungi-fermented oats demonstrated more effective inhibitory activity than that of nonfermented oats. A positive correlation between the total phenolics content and inhibitory activity was found. The inhibitory ability of the EA subfraction from nonfermented or fermented oats also displayed a dose-dependent effect. The standards of caffeic, ferulic, and p-coumaric acids, mainly included in EA Subfractions of fermented oats, also displayed a dose-dependent inhibitory effect. A synergistic effect of each binary combination of p-coumaric, ferulic, and caffeic acids was observed, especially at 150.0 μg/mL. Those results indicate that fungi-fermented oats have a more effective inhibitory ability on pancreatic lipase and polyphenols may be the most effective component and could be potentially used for dietary therapy of obesity.
Dimitra Daferera - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial and antioxidative activities of the essential oils and methanol extracts of salvia cryptantha montbret et aucher ex benth and salvia multicaulis vahl
Food Chemistry, 2004Co-Authors: Bektas Tepe, Gulhan Vardarunlu, Ferda Candan, Dimitra Daferera, Moschos G Polissiou, Erol Donmez, Mehmet Unlu, Atalay SokmenAbstract:Abstract The essential oils and methanolic extracts of Salvia cryptantha and Salvia multicaulis were examined for their potential antimicrobial and radical scavenging activities. No, or slight, activity was observed when the polar and non-polar Subfractions of the extracts were tested, whereas essential oils exhibited antimicrobial activity. The essential oils isolated from S. cryptantha and S. multicaulis were analysed by GC–MS and 53 and 47 constituents were identified, respectively. Antioxidant activities of the polar subfraction and the essential oil were examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical-scavenging and lipid peroxidation assays. The essential oils, in particular, and the non-polar Subfractions of methanol extracts, showed antioxidant activity. In conclusion, the results indicate that the oils of S. cryptantha and S. multicaulis have the capacity to scavenge free radicals and to inhibit the growth of pathogenic microorganisms. Therefore they could be suitable for using as antimicrobial and antioxidative agents in the food industry.
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in vitro antibacterial antifungal and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of satureja hortensis l
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Medine Gulluce, Atalay Sokmen, Dimitra Daferera, Moschos G Polissiou, Munevver Sokmen, Guleray Agar, Hakan Ozkan, N Kartal, Fikrettin SahinAbstract:The present study was designated to evaluate the antimicrobial and antioxidant activities of the essential oil, obtained by using a Clevenger distillation apparatus, water soluble (polar) and water insoluble (nonpolar) Subfractions of the methanol extracts from aerial parts of Satureja hortensis L. plants, and methanol extract from calli established from the seeds using Gamborg's B5 basal media supplemented with indole-3-butyric acid (1.0 ppm), 6-benzylaminopurine (N6-benzyladenine) (1.0 ppm), and sucrose (2.5%). The antimicrobial test results showed that the essential oil of S. hortensis had great potential antimicrobial activities against all 23 bacteria and 15 fungi and yeast species tested. In contrast, the methanol extract from callus cultures and water soluble subfraction of the methanol extract did not show antimicrobial activities, but the nonpolar subfraction had antibacterial activity against only five out of 23 bacterial species, which were Bacillus subtilis, Enterococcus fecalis, Pseudomonas a...
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in vitro antibacterial antifungal and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of satureja hortensis l
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Medine Gulluce, Atalay Sokmen, Dimitra Daferera, Moschos G Polissiou, Munevver Sokmen, Guleray Agar, Hakan Ozkan, N Kartal, Fikrettin SahinAbstract:The present study was designated to evaluate the antimicrobial and antioxidant activities of the essential oil, obtained by using a Clevenger distillation apparatus, water soluble (polar) and water insoluble (nonpolar) Subfractions of the methanol extracts from aerial parts of Satureja hortensis L. plants, and methanol extract from calli established from the seeds using Gamborg's B5 basal media supplemented with indole-3-butyric acid (1.0 ppm), 6-benzylaminopurine (N(6)-benzyladenine) (1.0 ppm), and sucrose (2.5%). The antimicrobial test results showed that the essential oil of S. hortensis had great potential antimicrobial activities against all 23 bacteria and 15 fungi and yeast species tested. In contrast, the methanol extract from callus cultures and water soluble subfraction of the methanol extract did not show antimicrobial activities, but the nonpolar subfraction had antibacterial activity against only five out of 23 bacterial species, which were Bacillus subtilis, Enterococcus fecalis, Pseudomonas aeruginosa, Salmonella enteritidis, and Streptococcus pyogenes. Antioxidant studies suggested that the polar Subfractions of the methanol extract of intact plant and methanol extract of callus cultures were able to reduce the stable free radical 2,2-diphenyl-1-picrylhydrazyl to the yellow-colored diphenylpicrylhydrazine. In this assay, the strongest effect was observed for the tissue culture extract, with an IC(50) value of 23.76 +/- 0.80 microgram/mL, which could be compared with the synthetic antioxidant agent butylated hydroxytoluene. On the other hand, linoleic acid oxidation was 95% inhibited in the presence of the essential oil while the inhibition was 90% with the chloroform subfraction of the intact plant. The chemical composition of a hydrodistilled essential oil of S. hortensis was analyzed by gas chromatography (GC)/flame ionization detection (FID) and a GC-mass spectrometry system. A total 22 constituents representing 99.9% of the essential oil were identified by GC-FID analaysis. Thymol (29.0%), carvacrol (26.5%), gamma-terpinene (22.6%), and p-cymene (9.3%) were the main components.
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antimicrobial and antioxidant activity of the essential oil and methanol extracts of thymus pectinatus fisch et mey var pectinatus lamiaceae
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Gulhan Vardarunlu, Ferda Candan, Atalay Sokmen, Dimitra Daferera, Moschos G Polissiou, Munevver Sokmen, Erol Donmez, Bektas TepeAbstract:The essential oil, obtained by using a Clevenger distillation apparatus, and water-soluble (polar) and water-insoluble (nonpolar) Subfractions of the methanol extract of Thymus pectinatus Fisch. et Mey. var. pectinatus were assayed for their antimicrobial and antioxidant properties. No (or slight) antimicrobial activity was observed when the Subfractions were tested, whereas the essential oil showed strong antimicrobial activity against all microorganisms tested. Antioxidant activities of the polar subfraction and the essential oil were evaluated using 2,2-diphenyl-1-picrylhydrazyl, hydroxyl radical, superoxide radical scavenging, and lipid peroxidation assays. The essential oil, in particular, and the polar subfraction of the methanol extract showed antioxidant activity. The essential oil was analyzed by GC/MS, and 24 compounds, representing 99.6% of the essential oil, were identified: thymol, gamma-terpinene, p-cymene, carvacrol, and borneol were the main components. An antimicrobial activity test carried out with fractions of the essential oil showed that the activity was mainly observed in those fractions containing thymol, in particular, and carvacrol. The activity was, therefore, attributed to the presence of these compounds. Other constituents of the essential oil, such as borneol, gamma-terpinene, and p-cymene, could be also taken into account for their possible synergistic or antagonistic effects. On the other hand, thymol and carvacrol were individually found to possess weaker antioxidant activity than the crude oil itself, indicating that other constituents of the essential oil may contribute to the antioxidant activity observed. In conclusion, the results presented here show that T. pectinatus essential oil could be considered as a natural antimicrobial and antioxidant source.
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antimicrobial and antioxidant activity of the essential oil and methanol extracts of thymus pectinatus fisch et mey var pectinatus lamiaceae
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Gulhan Vardarunlu, Ferda Candan, Atalay Sokmen, Dimitra Daferera, Moschos G Polissiou, Munevver Sokmen, Erol Donmez, Bektas TepeAbstract:The essential oil, obtained by using a Clevenger distillation apparatus, and water-soluble (polar) and water-insoluble (nonpolar) Subfractions of the methanol extract of Thymus pectinatus Fisch. et Mey. var. pectinatus were assayed for their antimicrobial and antioxidant properties. No (or slight) antimicrobial activity was observed when the Subfractions were tested, whereas the essential oil showed strong antimicrobial activity against all microorganisms tested. Antioxidant activities of the polar subfraction and the essential oil were evaluated using 2,2-diphenyl-1-picrylhydrazyl, hydroxyl radical, superoxide radical scavenging, and lipid peroxidation assays. The essential oil, in particular, and the polar subfraction of the methanol extract showed antioxidant activity. The essential oil was analyzed by GC/MS, and 24 compounds, representing 99.6% of the essential oil, were identified: thymol, γ-terpinene, p-cymene, carvacrol, and borneol were the main components. An antimicrobial activity test carried ...
Moschos G Polissiou - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial and antioxidative activities of the essential oils and methanol extracts of salvia cryptantha montbret et aucher ex benth and salvia multicaulis vahl
Food Chemistry, 2004Co-Authors: Bektas Tepe, Gulhan Vardarunlu, Ferda Candan, Dimitra Daferera, Moschos G Polissiou, Erol Donmez, Mehmet Unlu, Atalay SokmenAbstract:Abstract The essential oils and methanolic extracts of Salvia cryptantha and Salvia multicaulis were examined for their potential antimicrobial and radical scavenging activities. No, or slight, activity was observed when the polar and non-polar Subfractions of the extracts were tested, whereas essential oils exhibited antimicrobial activity. The essential oils isolated from S. cryptantha and S. multicaulis were analysed by GC–MS and 53 and 47 constituents were identified, respectively. Antioxidant activities of the polar subfraction and the essential oil were examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical-scavenging and lipid peroxidation assays. The essential oils, in particular, and the non-polar Subfractions of methanol extracts, showed antioxidant activity. In conclusion, the results indicate that the oils of S. cryptantha and S. multicaulis have the capacity to scavenge free radicals and to inhibit the growth of pathogenic microorganisms. Therefore they could be suitable for using as antimicrobial and antioxidative agents in the food industry.
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in vitro antibacterial antifungal and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of satureja hortensis l
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Medine Gulluce, Atalay Sokmen, Dimitra Daferera, Moschos G Polissiou, Munevver Sokmen, Guleray Agar, Hakan Ozkan, N Kartal, Fikrettin SahinAbstract:The present study was designated to evaluate the antimicrobial and antioxidant activities of the essential oil, obtained by using a Clevenger distillation apparatus, water soluble (polar) and water insoluble (nonpolar) Subfractions of the methanol extracts from aerial parts of Satureja hortensis L. plants, and methanol extract from calli established from the seeds using Gamborg's B5 basal media supplemented with indole-3-butyric acid (1.0 ppm), 6-benzylaminopurine (N6-benzyladenine) (1.0 ppm), and sucrose (2.5%). The antimicrobial test results showed that the essential oil of S. hortensis had great potential antimicrobial activities against all 23 bacteria and 15 fungi and yeast species tested. In contrast, the methanol extract from callus cultures and water soluble subfraction of the methanol extract did not show antimicrobial activities, but the nonpolar subfraction had antibacterial activity against only five out of 23 bacterial species, which were Bacillus subtilis, Enterococcus fecalis, Pseudomonas a...
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in vitro antibacterial antifungal and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of satureja hortensis l
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Medine Gulluce, Atalay Sokmen, Dimitra Daferera, Moschos G Polissiou, Munevver Sokmen, Guleray Agar, Hakan Ozkan, N Kartal, Fikrettin SahinAbstract:The present study was designated to evaluate the antimicrobial and antioxidant activities of the essential oil, obtained by using a Clevenger distillation apparatus, water soluble (polar) and water insoluble (nonpolar) Subfractions of the methanol extracts from aerial parts of Satureja hortensis L. plants, and methanol extract from calli established from the seeds using Gamborg's B5 basal media supplemented with indole-3-butyric acid (1.0 ppm), 6-benzylaminopurine (N(6)-benzyladenine) (1.0 ppm), and sucrose (2.5%). The antimicrobial test results showed that the essential oil of S. hortensis had great potential antimicrobial activities against all 23 bacteria and 15 fungi and yeast species tested. In contrast, the methanol extract from callus cultures and water soluble subfraction of the methanol extract did not show antimicrobial activities, but the nonpolar subfraction had antibacterial activity against only five out of 23 bacterial species, which were Bacillus subtilis, Enterococcus fecalis, Pseudomonas aeruginosa, Salmonella enteritidis, and Streptococcus pyogenes. Antioxidant studies suggested that the polar Subfractions of the methanol extract of intact plant and methanol extract of callus cultures were able to reduce the stable free radical 2,2-diphenyl-1-picrylhydrazyl to the yellow-colored diphenylpicrylhydrazine. In this assay, the strongest effect was observed for the tissue culture extract, with an IC(50) value of 23.76 +/- 0.80 microgram/mL, which could be compared with the synthetic antioxidant agent butylated hydroxytoluene. On the other hand, linoleic acid oxidation was 95% inhibited in the presence of the essential oil while the inhibition was 90% with the chloroform subfraction of the intact plant. The chemical composition of a hydrodistilled essential oil of S. hortensis was analyzed by gas chromatography (GC)/flame ionization detection (FID) and a GC-mass spectrometry system. A total 22 constituents representing 99.9% of the essential oil were identified by GC-FID analaysis. Thymol (29.0%), carvacrol (26.5%), gamma-terpinene (22.6%), and p-cymene (9.3%) were the main components.
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antimicrobial and antioxidant activity of the essential oil and methanol extracts of thymus pectinatus fisch et mey var pectinatus lamiaceae
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Gulhan Vardarunlu, Ferda Candan, Atalay Sokmen, Dimitra Daferera, Moschos G Polissiou, Munevver Sokmen, Erol Donmez, Bektas TepeAbstract:The essential oil, obtained by using a Clevenger distillation apparatus, and water-soluble (polar) and water-insoluble (nonpolar) Subfractions of the methanol extract of Thymus pectinatus Fisch. et Mey. var. pectinatus were assayed for their antimicrobial and antioxidant properties. No (or slight) antimicrobial activity was observed when the Subfractions were tested, whereas the essential oil showed strong antimicrobial activity against all microorganisms tested. Antioxidant activities of the polar subfraction and the essential oil were evaluated using 2,2-diphenyl-1-picrylhydrazyl, hydroxyl radical, superoxide radical scavenging, and lipid peroxidation assays. The essential oil, in particular, and the polar subfraction of the methanol extract showed antioxidant activity. The essential oil was analyzed by GC/MS, and 24 compounds, representing 99.6% of the essential oil, were identified: thymol, gamma-terpinene, p-cymene, carvacrol, and borneol were the main components. An antimicrobial activity test carried out with fractions of the essential oil showed that the activity was mainly observed in those fractions containing thymol, in particular, and carvacrol. The activity was, therefore, attributed to the presence of these compounds. Other constituents of the essential oil, such as borneol, gamma-terpinene, and p-cymene, could be also taken into account for their possible synergistic or antagonistic effects. On the other hand, thymol and carvacrol were individually found to possess weaker antioxidant activity than the crude oil itself, indicating that other constituents of the essential oil may contribute to the antioxidant activity observed. In conclusion, the results presented here show that T. pectinatus essential oil could be considered as a natural antimicrobial and antioxidant source.
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antimicrobial and antioxidant activity of the essential oil and methanol extracts of thymus pectinatus fisch et mey var pectinatus lamiaceae
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Gulhan Vardarunlu, Ferda Candan, Atalay Sokmen, Dimitra Daferera, Moschos G Polissiou, Munevver Sokmen, Erol Donmez, Bektas TepeAbstract:The essential oil, obtained by using a Clevenger distillation apparatus, and water-soluble (polar) and water-insoluble (nonpolar) Subfractions of the methanol extract of Thymus pectinatus Fisch. et Mey. var. pectinatus were assayed for their antimicrobial and antioxidant properties. No (or slight) antimicrobial activity was observed when the Subfractions were tested, whereas the essential oil showed strong antimicrobial activity against all microorganisms tested. Antioxidant activities of the polar subfraction and the essential oil were evaluated using 2,2-diphenyl-1-picrylhydrazyl, hydroxyl radical, superoxide radical scavenging, and lipid peroxidation assays. The essential oil, in particular, and the polar subfraction of the methanol extract showed antioxidant activity. The essential oil was analyzed by GC/MS, and 24 compounds, representing 99.6% of the essential oil, were identified: thymol, γ-terpinene, p-cymene, carvacrol, and borneol were the main components. An antimicrobial activity test carried ...
