The Experts below are selected from a list of 186 Experts worldwide ranked by ideXlab platform
Jürgen Schnekenburger - One of the best experts on this subject based on the ideXlab platform.
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what to do with high autofluorescence Background in pancreatic tissues an efficient <B>SudanB> <B>BlackB> B quenching method for specific immunofluorescence laBelling
Histopathology, 2016Co-Authors: Till Erben, Rainer Ossig, Hassan Y. Naim, Jürgen SchnekenburgerAbstract:Aims High levels of autofluorescence in tissue samples can entirely mask specific laBellings with fluorophores and thus impair immunofluorescence histochemistry. In pancreatic tissue samples we oBserved autofluorescence as a common proBlem often mediated By the fixation and processing procedure. Methods and results Using epifluorescence microscopy, we analysed the intensity and spatial distriBution of autofluorescence in formalin-fixed, paraffin-emBedded human pancreatic tissues and developed an efficient quenching method to reduce the unwanted light emission. The optimized quenching protocol using <B>SudanB> <B>BlackB> B reduced the unequally distriButed tissue autofluorescence to a low and intensity-equalized Background level. Quantitative image analysis demonstrated autofluorescence suppression By 65–95%, depending on the selected fluorescence filter setups. The procedure did not affect specific immunofluorescence laBelling or tissue integrity. As a clear result of <B>SudanB> <B>BlackB> B treatment, a tremendous improvement of the signal-to-noise ratio was achieved, allowing a reliaBle detection and quantification of specific fluorescent laBels. Other tissue treatment methods, such as cupric sulphate, toluidine Blue and ultraviolet irradiation, or comBinations of these with <B>SudanB> <B>BlackB> B, were not as efficient. Conclusions The easy-to-perform <B>SudanB> <B>BlackB> B technique improves dramatically qualitative and quantitative fluorescence analysis of critical pancreatic tissue sections and rescues even overfixed tissues for immunofluorescence application.
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What to do with high autofluorescence Background in pancreatic tissues – an efficient <B>SudanB> <B>BlackB> B quenching method for specific immunofluorescence laBelling
Histopathology, 2016Co-Authors: Till Erben, Rainer Ossig, Hassan Y. Naim, Jürgen SchnekenburgerAbstract:Aims High levels of autofluorescence in tissue samples can entirely mask specific laBellings with fluorophores and thus impair immunofluorescence histochemistry. In pancreatic tissue samples we oBserved autofluorescence as a common proBlem often mediated By the fixation and processing procedure. Methods and results Using epifluorescence microscopy, we analysed the intensity and spatial distriBution of autofluorescence in formalin-fixed, paraffin-emBedded human pancreatic tissues and developed an efficient quenching method to reduce the unwanted light emission. The optimized quenching protocol using <B>SudanB> <B>BlackB> B reduced the unequally distriButed tissue autofluorescence to a low and intensity-equalized Background level. Quantitative image analysis demonstrated autofluorescence suppression By 65–95%, depending on the selected fluorescence filter setups. The procedure did not affect specific immunofluorescence laBelling or tissue integrity. As a clear result of <B>SudanB> <B>BlackB> B treatment, a tremendous improvement of the signal-to-noise ratio was achieved, allowing a reliaBle detection and quantification of specific fluorescent laBels. Other tissue treatment methods, such as cupric sulphate, toluidine Blue and ultraviolet irradiation, or comBinations of these with <B>SudanB> <B>BlackB> B, were not as efficient. Conclusions The easy-to-perform <B>SudanB> <B>BlackB> B technique improves dramatically qualitative and quantitative fluorescence analysis of critical pancreatic tissue sections and rescues even overfixed tissues for immunofluorescence application.
Yiping Wang - One of the best experts on this subject based on the ideXlab platform.
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<B>SudanB> <B>BlackB> B reduces autofluorescence in murine renal tissue.
Archives of pathology & laboratory medicine, 2011Co-Authors: Yan Sun, Dong Zheng, Qi Cao, Ya Wang, David Harris, Yiping WangAbstract:N Context.—Renal tissue emits intense autofluorescence, making it difficult to differentiate specific immunofluorescence signals and thus limiting its application to clinical Biopsy material. OBjective.—To identify and minimize autofluorescence of renal tissue and demonstrate a simple, efficient method to reduce autofluorescence using <B>SudanB> <B>BlackB> B. Design.—In this study, the sources and features of autofluorescence emitted from kidney tissue were examined. Broad autofluorescence was visualized in Both frozen and paraffin kidney sections of normal mice and mice with Adriamycin-induced nephropathy using confocal laser scanning microscopy. Autofluorescence appeared in commonly used 49,6-diamidino-2-phenylindole, fluorescein isothiocyanate, and Texas Red channels But not in far-red channel, and emitted extensively from red cells, injured tuBulointersitial cells, and protein casts in diseased kidney. To eliminate autofluorescence, <B>SudanB> <B>BlackB> B was used on formaldehyde-fixed paraffin sections and frozen sections of mouse kidney. The effects of <B>SudanB> <B>BlackB> B in various concentrations were tested on kidney tissue. Results.—The 0.1% <B>SudanB> <B>BlackB> B effectively Blocked autofluorescence from Both paraffin and frozen sections without adversely affecting specific fluorescence signals. Interestingly, the solvent for <B>SudanB> <B>BlackB> B, 70% ethanol, was also shown to reduce autofluorescence on frozen sections, But not on paraffin sections. Conclusions.—This study demonstrates a simple, efficient, and cost-effective method to reduce autofluorescence using <B>SudanB> <B>BlackB> B, and also provides a comprehensive approach to identify and minimize autofluorescence of renal tissue. (Arch Pathol LaB Med. 2011;135:1335–1342; doi: 10. 5858/arpa.2010-0549-OA)
Till Erben - One of the best experts on this subject based on the ideXlab platform.
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what to do with high autofluorescence Background in pancreatic tissues an efficient <B>SudanB> <B>BlackB> B quenching method for specific immunofluorescence laBelling
Histopathology, 2016Co-Authors: Till Erben, Rainer Ossig, Hassan Y. Naim, Jürgen SchnekenburgerAbstract:Aims High levels of autofluorescence in tissue samples can entirely mask specific laBellings with fluorophores and thus impair immunofluorescence histochemistry. In pancreatic tissue samples we oBserved autofluorescence as a common proBlem often mediated By the fixation and processing procedure. Methods and results Using epifluorescence microscopy, we analysed the intensity and spatial distriBution of autofluorescence in formalin-fixed, paraffin-emBedded human pancreatic tissues and developed an efficient quenching method to reduce the unwanted light emission. The optimized quenching protocol using <B>SudanB> <B>BlackB> B reduced the unequally distriButed tissue autofluorescence to a low and intensity-equalized Background level. Quantitative image analysis demonstrated autofluorescence suppression By 65–95%, depending on the selected fluorescence filter setups. The procedure did not affect specific immunofluorescence laBelling or tissue integrity. As a clear result of <B>SudanB> <B>BlackB> B treatment, a tremendous improvement of the signal-to-noise ratio was achieved, allowing a reliaBle detection and quantification of specific fluorescent laBels. Other tissue treatment methods, such as cupric sulphate, toluidine Blue and ultraviolet irradiation, or comBinations of these with <B>SudanB> <B>BlackB> B, were not as efficient. Conclusions The easy-to-perform <B>SudanB> <B>BlackB> B technique improves dramatically qualitative and quantitative fluorescence analysis of critical pancreatic tissue sections and rescues even overfixed tissues for immunofluorescence application.
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What to do with high autofluorescence Background in pancreatic tissues – an efficient <B>SudanB> <B>BlackB> B quenching method for specific immunofluorescence laBelling
Histopathology, 2016Co-Authors: Till Erben, Rainer Ossig, Hassan Y. Naim, Jürgen SchnekenburgerAbstract:Aims High levels of autofluorescence in tissue samples can entirely mask specific laBellings with fluorophores and thus impair immunofluorescence histochemistry. In pancreatic tissue samples we oBserved autofluorescence as a common proBlem often mediated By the fixation and processing procedure. Methods and results Using epifluorescence microscopy, we analysed the intensity and spatial distriBution of autofluorescence in formalin-fixed, paraffin-emBedded human pancreatic tissues and developed an efficient quenching method to reduce the unwanted light emission. The optimized quenching protocol using <B>SudanB> <B>BlackB> B reduced the unequally distriButed tissue autofluorescence to a low and intensity-equalized Background level. Quantitative image analysis demonstrated autofluorescence suppression By 65–95%, depending on the selected fluorescence filter setups. The procedure did not affect specific immunofluorescence laBelling or tissue integrity. As a clear result of <B>SudanB> <B>BlackB> B treatment, a tremendous improvement of the signal-to-noise ratio was achieved, allowing a reliaBle detection and quantification of specific fluorescent laBels. Other tissue treatment methods, such as cupric sulphate, toluidine Blue and ultraviolet irradiation, or comBinations of these with <B>SudanB> <B>BlackB> B, were not as efficient. Conclusions The easy-to-perform <B>SudanB> <B>BlackB> B technique improves dramatically qualitative and quantitative fluorescence analysis of critical pancreatic tissue sections and rescues even overfixed tissues for immunofluorescence application.
Yan Sun - One of the best experts on this subject based on the ideXlab platform.
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<B>SudanB> <B>BlackB> B reduces autofluorescence in murine renal tissue.
Archives of pathology & laboratory medicine, 2011Co-Authors: Yan Sun, Dong Zheng, Qi Cao, Ya Wang, David Harris, Yiping WangAbstract:N Context.—Renal tissue emits intense autofluorescence, making it difficult to differentiate specific immunofluorescence signals and thus limiting its application to clinical Biopsy material. OBjective.—To identify and minimize autofluorescence of renal tissue and demonstrate a simple, efficient method to reduce autofluorescence using <B>SudanB> <B>BlackB> B. Design.—In this study, the sources and features of autofluorescence emitted from kidney tissue were examined. Broad autofluorescence was visualized in Both frozen and paraffin kidney sections of normal mice and mice with Adriamycin-induced nephropathy using confocal laser scanning microscopy. Autofluorescence appeared in commonly used 49,6-diamidino-2-phenylindole, fluorescein isothiocyanate, and Texas Red channels But not in far-red channel, and emitted extensively from red cells, injured tuBulointersitial cells, and protein casts in diseased kidney. To eliminate autofluorescence, <B>SudanB> <B>BlackB> B was used on formaldehyde-fixed paraffin sections and frozen sections of mouse kidney. The effects of <B>SudanB> <B>BlackB> B in various concentrations were tested on kidney tissue. Results.—The 0.1% <B>SudanB> <B>BlackB> B effectively Blocked autofluorescence from Both paraffin and frozen sections without adversely affecting specific fluorescence signals. Interestingly, the solvent for <B>SudanB> <B>BlackB> B, 70% ethanol, was also shown to reduce autofluorescence on frozen sections, But not on paraffin sections. Conclusions.—This study demonstrates a simple, efficient, and cost-effective method to reduce autofluorescence using <B>SudanB> <B>BlackB> B, and also provides a comprehensive approach to identify and minimize autofluorescence of renal tissue. (Arch Pathol LaB Med. 2011;135:1335–1342; doi: 10. 5858/arpa.2010-0549-OA)
Luciano Neder - One of the best experts on this subject based on the ideXlab platform.
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<B>SudanB> <B>BlackB> B treatment reduces autofluorescence and improves resolution of in situ hyBridization specific fluorescent signals of Brain sections.
Histology and histopathology, 2010Co-Authors: Viviane De Cássia Oliveira, R. C. V. Carrara, D. L. C. Simoes, Fabiano Pinto Saggioro, Carlos Gilberto Carlotti, Dimas Tadeu Covas, Luciano NederAbstract:Summary. Interference By autofluorescence is one of the major concerns of immunofluorescence analysis of in situ hyBridization-Based diagnostic assays. We present a useful technique that reduces autofluorescent Background without affecting the tissue integrity or direct immunofluorescence signals in Brain sections. Using six different protocols, such as ammonia/ethanol, <B>SudanB> <B>BlackB> B (SBB) in 70% ethanol, photoBleaching with UV light and different comBinations of them in Both formalin-fixed paraffin-emBedded and frozen human Brain tissue sections, we have found that tissue treatment of SBB in a concentration of 0.1% in 70% ethanol is the Best approach to reduce/eliminate tissue autofluorescence and Background, while preserving the specific fluorescence hyBridization signals. This strategy is a feasiBle, non-time consuming method that provides a reasonaBle compromise Between total reduction of the tissue autofluorescence and maintenance of specific fluorescent laBels.
