The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
S. Matsumoto - One of the best experts on this subject based on the ideXlab platform.
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Prostaglandin E_2 potentiates the excitability of small diameter trigeminal root ganglion neurons projecting onto the Superficial Layer of the cervical dorsal horn in rats
Experimental Brain Research, 2007Co-Authors: Jun Kadoi, M. Takeda, S. MatsumotoAbstract:The aim of the present study was to investigate how prostaglandin E_2 (PGE_2) affects the excitability of trigeminal root ganglion (TRG) neurons, projecting onto the Superficial Layer of the cervical dorsal horn, using fluorescence retrograde tracing and perforated patch-clamp techniques. TRG neurons were retrogradely labeled with fluorogold (FG). The cell diameter of FG-labeled neurons was small (
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Opioidergic modulation of excitability of rat trigeminal root ganglion neuron projections to the Superficial Layer of cervical dorsal horn.
Neuroscience, 2004Co-Authors: M. Takeda, Jun Kadoi, T. Tanimoto, Mizuho Ikeda, M. Nasu, S. MatsumotoAbstract:Abstract The aim of the present study was to investigate the effect of a μ-opioid receptor agonist DAMGO (Tyr- d -Ala-Gly-NMe-Phe-Gly-ol) on the excitability of trigeminal root ganglion (TRG) neurons, projecting onto the Superficial Layer of the cervical dorsal horn, by using the perforated-patch technique and to determine whether TRG neurons show the expression of mRNA or functional protein for μ-opioid receptors by using reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. TRG neurons projecting onto the Superficial Layer of the cervical dorsal horn were retrogradely labeled with Fluorogold (FG). The cell diameter of FG-labeled TRG neurons was small ( d -Phe-Cys-Thr- d -Trp-Orn-Thr-Pen-Thr-NH2). Hyperpolarization of the membrane potential was elicited by DAMGO (20 μM) and the response was associated with a decrease in the input resistance. DAMGO induced hyperpolarization was blocked by CTOP. DAMGO-sensitive IA and IK currents were antagonized by K+ channel blockers, 4-aminopyridine (4-AP) and tetraethylammonium (TEA). In the presence of both 4-AP and TEA, no significant changes in membrane potential induced by DAMGO application were observed. In the presence of BaCl2, DAMGO evoked hyperpolarization with decreased resistance was observed. The firing rate of action potentials and the first spike duration induced by depolarizing step pulses were decreased in the presence of DAMGO. RT-PCR analysis demonstrated the expression of mRNA for μ-opioid receptors in the trigeminal ganglia. The μ-opioid receptor immunoreactivity was expressed in the small diameter FG-labeled TRG neurons. These results suggest that the activation of μ-opioid receptors inhibits the excitability of rat small diameter TRG neurons projecting on the Superficial Layer of the cervical dorsal horn and this inhibition is mediated by potentiation of voltage-dependent K+ currents. We therefore concluded that modulation of nociceptive transmission in the trigeminal system, resulting in the functional activation of μ-opioid receptors, occurs at the level of small TRG cell bodies and/or their primary afferent terminals, which contribute to opioid analgesia in the trigeminal pain.
Jiming Chen - One of the best experts on this subject based on the ideXlab platform.
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Superficial Layer MHD Effect and Full-Cover Free Surface Flow Characterizing
World Journal of Nuclear Science and Technology, 2014Co-Authors: Zengyu Xu, X. D. Zhang, Jiming ChenAbstract:Up to now, no a real full-cover liquid metal (LM) free surface flow have been successfully used in magnetic fusion devices as MHD instability and unavoidable rivulet flow. Recently, after we carried out a guidable free curve-surface flow on theoretically and experimentally, seeking for other way to get a full-cover free surface flow is also in implementing. The Superficial Layer MHD effect in free surface flow is experimentally observed. After compared and analyzed the characteristic parameters of the free surface flow, the conditions of full-cover free surface flow are found. Meanwhile, the new two parameters of surface cover ratio and rivulet flow index are introduced to characterize the flowing characteristic of the full-cover free surface flow under magnetic field. According to the analysis rule, for different liquid metal, there are the different unique conditions to meet full-cover free surface flow under magnetic field. This may be a way to solve free surface flow major MHD key issue for LM PFCs.
Jun Kadoi - One of the best experts on this subject based on the ideXlab platform.
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Prostaglandin E_2 potentiates the excitability of small diameter trigeminal root ganglion neurons projecting onto the Superficial Layer of the cervical dorsal horn in rats
Experimental Brain Research, 2007Co-Authors: Jun Kadoi, M. Takeda, S. MatsumotoAbstract:The aim of the present study was to investigate how prostaglandin E_2 (PGE_2) affects the excitability of trigeminal root ganglion (TRG) neurons, projecting onto the Superficial Layer of the cervical dorsal horn, using fluorescence retrograde tracing and perforated patch-clamp techniques. TRG neurons were retrogradely labeled with fluorogold (FG). The cell diameter of FG-labeled neurons was small (
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Opioidergic modulation of excitability of rat trigeminal root ganglion neuron projections to the Superficial Layer of cervical dorsal horn.
Neuroscience, 2004Co-Authors: M. Takeda, Jun Kadoi, T. Tanimoto, Mizuho Ikeda, M. Nasu, S. MatsumotoAbstract:Abstract The aim of the present study was to investigate the effect of a μ-opioid receptor agonist DAMGO (Tyr- d -Ala-Gly-NMe-Phe-Gly-ol) on the excitability of trigeminal root ganglion (TRG) neurons, projecting onto the Superficial Layer of the cervical dorsal horn, by using the perforated-patch technique and to determine whether TRG neurons show the expression of mRNA or functional protein for μ-opioid receptors by using reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. TRG neurons projecting onto the Superficial Layer of the cervical dorsal horn were retrogradely labeled with Fluorogold (FG). The cell diameter of FG-labeled TRG neurons was small ( d -Phe-Cys-Thr- d -Trp-Orn-Thr-Pen-Thr-NH2). Hyperpolarization of the membrane potential was elicited by DAMGO (20 μM) and the response was associated with a decrease in the input resistance. DAMGO induced hyperpolarization was blocked by CTOP. DAMGO-sensitive IA and IK currents were antagonized by K+ channel blockers, 4-aminopyridine (4-AP) and tetraethylammonium (TEA). In the presence of both 4-AP and TEA, no significant changes in membrane potential induced by DAMGO application were observed. In the presence of BaCl2, DAMGO evoked hyperpolarization with decreased resistance was observed. The firing rate of action potentials and the first spike duration induced by depolarizing step pulses were decreased in the presence of DAMGO. RT-PCR analysis demonstrated the expression of mRNA for μ-opioid receptors in the trigeminal ganglia. The μ-opioid receptor immunoreactivity was expressed in the small diameter FG-labeled TRG neurons. These results suggest that the activation of μ-opioid receptors inhibits the excitability of rat small diameter TRG neurons projecting on the Superficial Layer of the cervical dorsal horn and this inhibition is mediated by potentiation of voltage-dependent K+ currents. We therefore concluded that modulation of nociceptive transmission in the trigeminal system, resulting in the functional activation of μ-opioid receptors, occurs at the level of small TRG cell bodies and/or their primary afferent terminals, which contribute to opioid analgesia in the trigeminal pain.
Tie Wang - One of the best experts on this subject based on the ideXlab platform.
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Superficial Layer enhanced raman scattering slers for depth detection of noncontact molecules
Advanced Materials, 2019Co-Authors: Xuezhi Qiao, Zhenjie Xue, Lu Liu, Keyan Liu, Tie WangAbstract:Although the strength of Raman signals can be increased by many orders of magnitude on noble metal nanoparticles, this enhancement is confined to an extremely short distance from the Raman-active surface. The key to the development of Raman spectroscopy for applications in diagnosis and detection of cancer and inflammatory diseases, and in pharmacology, relies on the capability of detecting analytes that are noninteractive with Raman-active surfaces. Here, a new Raman enhancement system is constructed, Superficial-Layer-enhanced Raman scattering (SLERS), by covering elongated tetrahexahedral gold nanoparticle arrays with a Superficial perovskite (CH3 NH3 PbBr3 ) film. Plasmonic decay is depressed along the vertical direction away from the noble metal surface and the penetration depth is increased in the perovskite media. The vertical penetration of SLERS is verified by the spatial distribution of the analytes via Raman imaging in Layer-scanning mode.
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Superficial‐Layer‐Enhanced Raman Scattering (SLERS) for Depth Detection of Noncontact Molecules
Advanced materials (Deerfield Beach Fla.), 2018Co-Authors: Xuezhi Qiao, Zhenjie Xue, Lu Liu, Keyan Liu, Tie WangAbstract:Although the strength of Raman signals can be increased by many orders of magnitude on noble metal nanoparticles, this enhancement is confined to an extremely short distance from the Raman-active surface. The key to the development of Raman spectroscopy for applications in diagnosis and detection of cancer and inflammatory diseases, and in pharmacology, relies on the capability of detecting analytes that are noninteractive with Raman-active surfaces. Here, a new Raman enhancement system is constructed, Superficial-Layer-enhanced Raman scattering (SLERS), by covering elongated tetrahexahedral gold nanoparticle arrays with a Superficial perovskite (CH3 NH3 PbBr3 ) film. Plasmonic decay is depressed along the vertical direction away from the noble metal surface and the penetration depth is increased in the perovskite media. The vertical penetration of SLERS is verified by the spatial distribution of the analytes via Raman imaging in Layer-scanning mode.
M. Takeda - One of the best experts on this subject based on the ideXlab platform.
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Prostaglandin E_2 potentiates the excitability of small diameter trigeminal root ganglion neurons projecting onto the Superficial Layer of the cervical dorsal horn in rats
Experimental Brain Research, 2007Co-Authors: Jun Kadoi, M. Takeda, S. MatsumotoAbstract:The aim of the present study was to investigate how prostaglandin E_2 (PGE_2) affects the excitability of trigeminal root ganglion (TRG) neurons, projecting onto the Superficial Layer of the cervical dorsal horn, using fluorescence retrograde tracing and perforated patch-clamp techniques. TRG neurons were retrogradely labeled with fluorogold (FG). The cell diameter of FG-labeled neurons was small (
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Opioidergic modulation of excitability of rat trigeminal root ganglion neuron projections to the Superficial Layer of cervical dorsal horn.
Neuroscience, 2004Co-Authors: M. Takeda, Jun Kadoi, T. Tanimoto, Mizuho Ikeda, M. Nasu, S. MatsumotoAbstract:Abstract The aim of the present study was to investigate the effect of a μ-opioid receptor agonist DAMGO (Tyr- d -Ala-Gly-NMe-Phe-Gly-ol) on the excitability of trigeminal root ganglion (TRG) neurons, projecting onto the Superficial Layer of the cervical dorsal horn, by using the perforated-patch technique and to determine whether TRG neurons show the expression of mRNA or functional protein for μ-opioid receptors by using reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. TRG neurons projecting onto the Superficial Layer of the cervical dorsal horn were retrogradely labeled with Fluorogold (FG). The cell diameter of FG-labeled TRG neurons was small ( d -Phe-Cys-Thr- d -Trp-Orn-Thr-Pen-Thr-NH2). Hyperpolarization of the membrane potential was elicited by DAMGO (20 μM) and the response was associated with a decrease in the input resistance. DAMGO induced hyperpolarization was blocked by CTOP. DAMGO-sensitive IA and IK currents were antagonized by K+ channel blockers, 4-aminopyridine (4-AP) and tetraethylammonium (TEA). In the presence of both 4-AP and TEA, no significant changes in membrane potential induced by DAMGO application were observed. In the presence of BaCl2, DAMGO evoked hyperpolarization with decreased resistance was observed. The firing rate of action potentials and the first spike duration induced by depolarizing step pulses were decreased in the presence of DAMGO. RT-PCR analysis demonstrated the expression of mRNA for μ-opioid receptors in the trigeminal ganglia. The μ-opioid receptor immunoreactivity was expressed in the small diameter FG-labeled TRG neurons. These results suggest that the activation of μ-opioid receptors inhibits the excitability of rat small diameter TRG neurons projecting on the Superficial Layer of the cervical dorsal horn and this inhibition is mediated by potentiation of voltage-dependent K+ currents. We therefore concluded that modulation of nociceptive transmission in the trigeminal system, resulting in the functional activation of μ-opioid receptors, occurs at the level of small TRG cell bodies and/or their primary afferent terminals, which contribute to opioid analgesia in the trigeminal pain.
