Supernatant

14,000,000 Leading Edge Experts on the ideXlab platform

Scan Science and Technology

Contact Leading Edge Experts & Companies

Scan Science and Technology

Contact Leading Edge Experts & Companies

The Experts below are selected from a list of 252 Experts worldwide ranked by ideXlab platform

Christopher C Silliman - One of the best experts on this subject based on the ideXlab platform.

  • proteomic analysis of the Supernatant of red blood cell units the effects of storage and leucoreduction
    Vox Sanguinis, 2013
    Co-Authors: Monika Dzieciatkowska, Anirban Banerjee, Christopher C Silliman, Ernest E Moore, Marguerite R Kelher, Kevin J Land, Michael A Ellison, F B West
    Abstract:

    Background Red blood cell (RBC) transfusion is a life-saving intervention for critically ill patients; however, it has been linked to increased morbidity and mortality. We hypothesize that a number of important proteins accumulate during routine storage of RBCs, which may explain some of the adverse effects seen in transfused patients. Study Design Five RBC units were drawn and divided (half prestorage leucoreduced (LR-RBC) and half left as an unmodified control (RBC). The Supernatant was separated on days 1 and 42 of storage and proteomic analyses completed with in-gel tryptic digestion and nano-liquid chromatography tandem mass spectrometry. Results In RBC Supernatants, 401 proteins were identified: 203 increased with storage, 114 decreased, and 84 were unchanged. In LR-RBC Supernatant, 231 proteins were identified: 84 increased with storage, 30 decreased, and 117 were unchanged. Prestorage leucoreduction removed many platelet- and leucocyte-derived structural proteins; however, a number of intracellular proteins accumulated including peroxiredoxins (Prdx) 6 and latexin. The increases were confirmed by immunoblotting, including the T-phosphorylation of Prdx-6, indicating that it may be functioning as an active phospholipase. Active matrix metalloproteinase-9 also increased with a coinciding decrease in the metalloproteinase inhibitor 1 and cystatin C. Conclusion We conclude that a number of proteins increase with RBC storage, which is partially ameliorated with leucoreduction, and transfusion of stored RBCs may introduce mediators that result in adverse events in the transfused host.

  • red blood cell Supernatant potentiates lps induced proinflammatory cytokine response from peripheral blood mononuclear cells
    Journal of Interferon and Cytokine Research, 2009
    Co-Authors: Joel M Baumgartner, Trevor L Nydam, Jason H Clarke, Anirban Banerjee, Christopher C Silliman, Martin D Mccarter
    Abstract:

    Allogeneic blood transfusion has an immunomodulatory capacity on its recipients through accumulation of immunologically active substances with blood storage, and prestorage leukoreduction reduces many of these mediators. We investigated lipopolysaccharide (LPS)-induced cytokine response of peripheral blood mononuclear cells (PBMCs) exposed to packed red blood cell (PRBC) Supernatants from leukoreduced (LR) or non-leukoreduced (NLR) units with variable duration of storage. PRBC units were collected with or without leukoreduction on Day 0 before routine storage. The plasma fraction (Supernatant) was isolated from LR and NLR units after 1 day (D1) or 42 days (D42) of storage and exposed to PBMCs versus control media for 24 h, then with LPS for an additional 24 h. Cell Supernatants were analyzed for IL-1β, IL-6, IL-8, IL-10, and TNF-α by cytokine bead array. IL-1β, TNF-α, and IL-6 were significantly elevated in PRBC groups versus control. D42 NLR PRBC Supernatant significantly increased secretion of IL-1β and IL-6 compared to D1 NLR PRBC Supernatant. LR significantly attenuated the cytokine response of IL-1β. Thus, PRBC Supernatant potentiates proinflammatory LPS-induced cytokine secretion from PBMCs. This response is accentuated with storage duration and partially attenuated with leukoreduction. These findings may partially explain the immune activation seen clinically after blood transfusion.

Martin D Mccarter - One of the best experts on this subject based on the ideXlab platform.

  • red blood cell Supernatant potentiates lps induced proinflammatory cytokine response from peripheral blood mononuclear cells
    Journal of Interferon and Cytokine Research, 2009
    Co-Authors: Joel M Baumgartner, Trevor L Nydam, Jason H Clarke, Anirban Banerjee, Christopher C Silliman, Martin D Mccarter
    Abstract:

    Allogeneic blood transfusion has an immunomodulatory capacity on its recipients through accumulation of immunologically active substances with blood storage, and prestorage leukoreduction reduces many of these mediators. We investigated lipopolysaccharide (LPS)-induced cytokine response of peripheral blood mononuclear cells (PBMCs) exposed to packed red blood cell (PRBC) Supernatants from leukoreduced (LR) or non-leukoreduced (NLR) units with variable duration of storage. PRBC units were collected with or without leukoreduction on Day 0 before routine storage. The plasma fraction (Supernatant) was isolated from LR and NLR units after 1 day (D1) or 42 days (D42) of storage and exposed to PBMCs versus control media for 24 h, then with LPS for an additional 24 h. Cell Supernatants were analyzed for IL-1β, IL-6, IL-8, IL-10, and TNF-α by cytokine bead array. IL-1β, TNF-α, and IL-6 were significantly elevated in PRBC groups versus control. D42 NLR PRBC Supernatant significantly increased secretion of IL-1β and IL-6 compared to D1 NLR PRBC Supernatant. LR significantly attenuated the cytokine response of IL-1β. Thus, PRBC Supernatant potentiates proinflammatory LPS-induced cytokine secretion from PBMCs. This response is accentuated with storage duration and partially attenuated with leukoreduction. These findings may partially explain the immune activation seen clinically after blood transfusion.

Huseyin Yilmaz - One of the best experts on this subject based on the ideXlab platform.

  • Polyelectrolyte Mediated Interaction of Alumina in Wet Jet Milled Slurry/Ball Milled Slurry Supernatants
    Journal of the Ceramic Society of Japan, 2006
    Co-Authors: Koji Watari, Toshihiro Isobe, Yuji Hotta, Huseyin Yilmaz, Kimiyasu Sato
    Abstract:

    In a recent paper by Omura et al.,1) wet jet milled α-alumina slurries exhibited distinctly different stability behavior compared to ball milled ones in terms of re-flocculation efficiency, rheological properties and packing density. The distinction was attributed to the different behavior of the same NH4+ salt of poly (acrylic acid) in the two milling methods used. Force measurement via colloid probe method in the centrifuged Supernatant of the milled slurries confirmed that the interaction distance between an alumina colloid probe and sapphire surface is about two times larger in wet jet milled slurry Supernatant compared to the just ball milled one. This result confirmed that processing variables do affect the polyelectrolyte behavior in the slurry. The net force was always repulsive on approach in either Supernatants.

  • polyelectrolyte mediated interaction of alumina in wet jet milled slurry ball milled slurry Supernatants
    Journal of the Ceramic Society of Japan, 2006
    Co-Authors: Toshihiro Isobe, Yuji Hotta, Huseyin Yilmaz, Kimiyasu Sato, Koji Watari
    Abstract:

    In a recent paper by Omura et al.,1) wet jet milled α-alumina slurries exhibited distinctly different stability behavior compared to ball milled ones in terms of re-flocculation efficiency, rheological properties and packing density. The distinction was attributed to the different behavior of the same NH4 + salt of poly(acrylic acid) in the two milling methods used. Force measurement via colloid probe method in the centrifuged Supernatant of the milled slurries confirmed that the interaction distance between an alumina colloid probe and sapphire surface is about two times larger in wet jet milled slurry Supernatant compared to the just ball milled one. This result confirmed that processing variables do affect the polyelectrolyte behavior in the slurry. The net force was always repulsive on approach in either Supernatants.

Anirban Banerjee - One of the best experts on this subject based on the ideXlab platform.

  • proteomic analysis of the Supernatant of red blood cell units the effects of storage and leucoreduction
    Vox Sanguinis, 2013
    Co-Authors: Monika Dzieciatkowska, Anirban Banerjee, Christopher C Silliman, Ernest E Moore, Marguerite R Kelher, Kevin J Land, Michael A Ellison, F B West
    Abstract:

    Background Red blood cell (RBC) transfusion is a life-saving intervention for critically ill patients; however, it has been linked to increased morbidity and mortality. We hypothesize that a number of important proteins accumulate during routine storage of RBCs, which may explain some of the adverse effects seen in transfused patients. Study Design Five RBC units were drawn and divided (half prestorage leucoreduced (LR-RBC) and half left as an unmodified control (RBC). The Supernatant was separated on days 1 and 42 of storage and proteomic analyses completed with in-gel tryptic digestion and nano-liquid chromatography tandem mass spectrometry. Results In RBC Supernatants, 401 proteins were identified: 203 increased with storage, 114 decreased, and 84 were unchanged. In LR-RBC Supernatant, 231 proteins were identified: 84 increased with storage, 30 decreased, and 117 were unchanged. Prestorage leucoreduction removed many platelet- and leucocyte-derived structural proteins; however, a number of intracellular proteins accumulated including peroxiredoxins (Prdx) 6 and latexin. The increases were confirmed by immunoblotting, including the T-phosphorylation of Prdx-6, indicating that it may be functioning as an active phospholipase. Active matrix metalloproteinase-9 also increased with a coinciding decrease in the metalloproteinase inhibitor 1 and cystatin C. Conclusion We conclude that a number of proteins increase with RBC storage, which is partially ameliorated with leucoreduction, and transfusion of stored RBCs may introduce mediators that result in adverse events in the transfused host.

  • red blood cell Supernatant potentiates lps induced proinflammatory cytokine response from peripheral blood mononuclear cells
    Journal of Interferon and Cytokine Research, 2009
    Co-Authors: Joel M Baumgartner, Trevor L Nydam, Jason H Clarke, Anirban Banerjee, Christopher C Silliman, Martin D Mccarter
    Abstract:

    Allogeneic blood transfusion has an immunomodulatory capacity on its recipients through accumulation of immunologically active substances with blood storage, and prestorage leukoreduction reduces many of these mediators. We investigated lipopolysaccharide (LPS)-induced cytokine response of peripheral blood mononuclear cells (PBMCs) exposed to packed red blood cell (PRBC) Supernatants from leukoreduced (LR) or non-leukoreduced (NLR) units with variable duration of storage. PRBC units were collected with or without leukoreduction on Day 0 before routine storage. The plasma fraction (Supernatant) was isolated from LR and NLR units after 1 day (D1) or 42 days (D42) of storage and exposed to PBMCs versus control media for 24 h, then with LPS for an additional 24 h. Cell Supernatants were analyzed for IL-1β, IL-6, IL-8, IL-10, and TNF-α by cytokine bead array. IL-1β, TNF-α, and IL-6 were significantly elevated in PRBC groups versus control. D42 NLR PRBC Supernatant significantly increased secretion of IL-1β and IL-6 compared to D1 NLR PRBC Supernatant. LR significantly attenuated the cytokine response of IL-1β. Thus, PRBC Supernatant potentiates proinflammatory LPS-induced cytokine secretion from PBMCs. This response is accentuated with storage duration and partially attenuated with leukoreduction. These findings may partially explain the immune activation seen clinically after blood transfusion.

Koji Watari - One of the best experts on this subject based on the ideXlab platform.

  • Polyelectrolyte Mediated Interaction of Alumina in Wet Jet Milled Slurry/Ball Milled Slurry Supernatants
    Journal of the Ceramic Society of Japan, 2006
    Co-Authors: Koji Watari, Toshihiro Isobe, Yuji Hotta, Huseyin Yilmaz, Kimiyasu Sato
    Abstract:

    In a recent paper by Omura et al.,1) wet jet milled α-alumina slurries exhibited distinctly different stability behavior compared to ball milled ones in terms of re-flocculation efficiency, rheological properties and packing density. The distinction was attributed to the different behavior of the same NH4+ salt of poly (acrylic acid) in the two milling methods used. Force measurement via colloid probe method in the centrifuged Supernatant of the milled slurries confirmed that the interaction distance between an alumina colloid probe and sapphire surface is about two times larger in wet jet milled slurry Supernatant compared to the just ball milled one. This result confirmed that processing variables do affect the polyelectrolyte behavior in the slurry. The net force was always repulsive on approach in either Supernatants.

  • polyelectrolyte mediated interaction of alumina in wet jet milled slurry ball milled slurry Supernatants
    Journal of the Ceramic Society of Japan, 2006
    Co-Authors: Toshihiro Isobe, Yuji Hotta, Huseyin Yilmaz, Kimiyasu Sato, Koji Watari
    Abstract:

    In a recent paper by Omura et al.,1) wet jet milled α-alumina slurries exhibited distinctly different stability behavior compared to ball milled ones in terms of re-flocculation efficiency, rheological properties and packing density. The distinction was attributed to the different behavior of the same NH4 + salt of poly(acrylic acid) in the two milling methods used. Force measurement via colloid probe method in the centrifuged Supernatant of the milled slurries confirmed that the interaction distance between an alumina colloid probe and sapphire surface is about two times larger in wet jet milled slurry Supernatant compared to the just ball milled one. This result confirmed that processing variables do affect the polyelectrolyte behavior in the slurry. The net force was always repulsive on approach in either Supernatants.