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Md Sakhawat Hossain - One of the best experts on this subject based on the ideXlab platform.
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dietary cytidine monophosphate enhances the growth blood characteristics innate and adaptive immune functions and stress resistance of juvenile red sea bream pagrus major
Aquaculture, 2017Co-Authors: Md Sakhawat Hossain, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Nadia Mahjabin SonyAbstract:Abstract The present study was designed to evaluate the effects of dietary cytidine monophosphate on growth, blood characteristics, innate and adaptive immune functions and stress resistance of juvenile red sea bream, Pagrus major . A semi-purified basal diet supplemented with 0% (Control), 0.1% (CMP-0.1), 0.2% (CMP-0.2), 0.4% (CMP-0.4) and 0.8% (CMP-0.8) purified CMP to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (mean initial weight 2.9 g) for 56 days. In general dietary CMP Supplementations tended to improve growth performances (final body weight, % weight gain and specific growth rate), in which significantly highest performances were found in diet group CMP-0.4 followed by diet groups CMP-0.8 and CMP-0.2. CMP Supplementation resulting in increased feed conversion efficiency and protein efficiency ratio and highest value (P 0.05) with dietary CMP Supplementations. Dietary Supplementation of CMP had an increase (P > 0.05) adaptive humoral immune responses (antibody titer) after 21 days of vaccination of formalin killed Vibrio anguillarum . In terms of oxidative stress diet groups CMP-0.2 and CMP-0.4 showed best condition with low oxidative stress and high antioxidant levels. Moreover, the fish fed CMP supplemented diets had better improvement (P
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dietary cytidine monophosphate enhances the growth blood characteristics innate and adaptive immune functions and stress resistance of juvenile red sea bream pagrus major
Aquaculture, 2017Co-Authors: Md Sakhawat Hossain, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Nadia Mahjabin SonyAbstract:Abstract The present study was designed to evaluate the effects of dietary cytidine monophosphate on growth, blood characteristics, innate and adaptive immune functions and stress resistance of juvenile red sea bream, Pagrus major . A semi-purified basal diet supplemented with 0% (Control), 0.1% (CMP-0.1), 0.2% (CMP-0.2), 0.4% (CMP-0.4) and 0.8% (CMP-0.8) purified CMP to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (mean initial weight 2.9 g) for 56 days. In general dietary CMP Supplementations tended to improve growth performances (final body weight, % weight gain and specific growth rate), in which significantly highest performances were found in diet group CMP-0.4 followed by diet groups CMP-0.8 and CMP-0.2. CMP Supplementation resulting in increased feed conversion efficiency and protein efficiency ratio and highest value (P 0.05) with dietary CMP Supplementations. Dietary Supplementation of CMP had an increase (P > 0.05) adaptive humoral immune responses (antibody titer) after 21 days of vaccination of formalin killed Vibrio anguillarum . In terms of oxidative stress diet groups CMP-0.2 and CMP-0.4 showed best condition with low oxidative stress and high antioxidant levels. Moreover, the fish fed CMP supplemented diets had better improvement (P
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comparison of the effects of inosine and inosine monophosphate on growth immune response stress resistance and gut morphology of juvenile red sea bream pagrus major
Aquaculture, 2016Co-Authors: Md Sakhawat Hossain, Nadia Mahjabin Sony, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Takeshi FujiedaAbstract:Abstract Inosine and inosine monophosphate (IMP) are the most extensively studied specific nucleotide for feeding stimulation research rather than using as functional nutrients for potential growth and health benefit of aquatic species. Therefore, a 10-week feeding trial was conducted to determine comparative effects of inosine and IMP as functional nutrients on growth performance, immune responses, stress resistance and intestinal morphology of juvenile red sea bream ( Pagrus major ). Casein based semi-purified basal diet was formulated to contain 54% protein, 11% lipid, without inosine and IMP Supplementation (Control). Four levels of inosine nucleoside (0.2, 0.4, 0.6 and 0.8% for diet groups INO-0.2, INO-0.4, INO-0.6 and INO-0.8 respectively) and IMP nucleotide (0.2, 0.4, 0.6 and 0.8% for diet groups IMP-0.2, IMP-0.4, IMP-0.6 and IMP-0.8 respectively) were added to the basal diet. Each diet was randomly allocated to triplicate groups of fish with initial average weight of 6.6 g. The results indicated that dietary inosine and IMP Supplementations tended to improve growth performances, in which one of the best ones was found in diet group IMP-0.6, but the values did not significantly differ from those in diet groups IMP-0.4 and INO-0.4, respectively. Superoxide dismutase, peroxidase and bactericidal activity were significantly influenced by dietary Supplementation of inosine and IMP. However, the values of these parameters were not significantly different among diet groups INO-0.4, IMP-0.4 and IMP-0.6. Total serum protein, catalase and lysozyme activity were also improved (P > 0.05) by dietary Supplementation of inosine and IMP. Supplementations improved both freshwater stress and oxidative stress resistances. Fish fed diet groups INO-0.4 and IMP-0.6 showed the least oxidative stress condition. Inosine and IMP Supplementations significantly increased enterocyte height (hE), fold height (hF) of both anterior and posterior intestine and microvillus height (hMV) of posterior intestine compared to those of the control. The highest hF observed in diet group IMP-0.4 and the mean value was not significantly different from those in other IMP diets, INO-0.4 and INO-0.6, respectively. Significantly highest anterior hE and posterior hF, hE and hMV observed in diet group INO-0.4. However, no significant difference of posterior hF observed between INO-0.4 and IMP supplemented diet groups. Fish fed supplemented diets also showed improved survival compared to control (P > 0.05). Considering overall performances, it can be concluded that diet groups INO-0.4 and IMP-0.4 showed relatively better performance among inosine and IMP supplemented diet groups respectively compared to control. Furthermore, diets supplemented with either inosine or IMP at 0.4% are beneficial to promote growth, immune responses, stress resistance and intestinal health condition of juvenile red sea bream. Statement of relevance In this study inosine and inosine monophosphate has been evaluated as potential functional nutrients for marine fish such as red sea bream. Since Supplementations of such immunostimulant-like functional nutrients are very important for the development of functional aquafeeds, I hope findings of this paper will help to develop low fishmeal based functional aquafeeds in near future.
Nadia Mahjabin Sony - One of the best experts on this subject based on the ideXlab platform.
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dietary cytidine monophosphate enhances the growth blood characteristics innate and adaptive immune functions and stress resistance of juvenile red sea bream pagrus major
Aquaculture, 2017Co-Authors: Md Sakhawat Hossain, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Nadia Mahjabin SonyAbstract:Abstract The present study was designed to evaluate the effects of dietary cytidine monophosphate on growth, blood characteristics, innate and adaptive immune functions and stress resistance of juvenile red sea bream, Pagrus major . A semi-purified basal diet supplemented with 0% (Control), 0.1% (CMP-0.1), 0.2% (CMP-0.2), 0.4% (CMP-0.4) and 0.8% (CMP-0.8) purified CMP to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (mean initial weight 2.9 g) for 56 days. In general dietary CMP Supplementations tended to improve growth performances (final body weight, % weight gain and specific growth rate), in which significantly highest performances were found in diet group CMP-0.4 followed by diet groups CMP-0.8 and CMP-0.2. CMP Supplementation resulting in increased feed conversion efficiency and protein efficiency ratio and highest value (P 0.05) with dietary CMP Supplementations. Dietary Supplementation of CMP had an increase (P > 0.05) adaptive humoral immune responses (antibody titer) after 21 days of vaccination of formalin killed Vibrio anguillarum . In terms of oxidative stress diet groups CMP-0.2 and CMP-0.4 showed best condition with low oxidative stress and high antioxidant levels. Moreover, the fish fed CMP supplemented diets had better improvement (P
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dietary cytidine monophosphate enhances the growth blood characteristics innate and adaptive immune functions and stress resistance of juvenile red sea bream pagrus major
Aquaculture, 2017Co-Authors: Md Sakhawat Hossain, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Nadia Mahjabin SonyAbstract:Abstract The present study was designed to evaluate the effects of dietary cytidine monophosphate on growth, blood characteristics, innate and adaptive immune functions and stress resistance of juvenile red sea bream, Pagrus major . A semi-purified basal diet supplemented with 0% (Control), 0.1% (CMP-0.1), 0.2% (CMP-0.2), 0.4% (CMP-0.4) and 0.8% (CMP-0.8) purified CMP to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (mean initial weight 2.9 g) for 56 days. In general dietary CMP Supplementations tended to improve growth performances (final body weight, % weight gain and specific growth rate), in which significantly highest performances were found in diet group CMP-0.4 followed by diet groups CMP-0.8 and CMP-0.2. CMP Supplementation resulting in increased feed conversion efficiency and protein efficiency ratio and highest value (P 0.05) with dietary CMP Supplementations. Dietary Supplementation of CMP had an increase (P > 0.05) adaptive humoral immune responses (antibody titer) after 21 days of vaccination of formalin killed Vibrio anguillarum . In terms of oxidative stress diet groups CMP-0.2 and CMP-0.4 showed best condition with low oxidative stress and high antioxidant levels. Moreover, the fish fed CMP supplemented diets had better improvement (P
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comparison of the effects of inosine and inosine monophosphate on growth immune response stress resistance and gut morphology of juvenile red sea bream pagrus major
Aquaculture, 2016Co-Authors: Md Sakhawat Hossain, Nadia Mahjabin Sony, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Takeshi FujiedaAbstract:Abstract Inosine and inosine monophosphate (IMP) are the most extensively studied specific nucleotide for feeding stimulation research rather than using as functional nutrients for potential growth and health benefit of aquatic species. Therefore, a 10-week feeding trial was conducted to determine comparative effects of inosine and IMP as functional nutrients on growth performance, immune responses, stress resistance and intestinal morphology of juvenile red sea bream ( Pagrus major ). Casein based semi-purified basal diet was formulated to contain 54% protein, 11% lipid, without inosine and IMP Supplementation (Control). Four levels of inosine nucleoside (0.2, 0.4, 0.6 and 0.8% for diet groups INO-0.2, INO-0.4, INO-0.6 and INO-0.8 respectively) and IMP nucleotide (0.2, 0.4, 0.6 and 0.8% for diet groups IMP-0.2, IMP-0.4, IMP-0.6 and IMP-0.8 respectively) were added to the basal diet. Each diet was randomly allocated to triplicate groups of fish with initial average weight of 6.6 g. The results indicated that dietary inosine and IMP Supplementations tended to improve growth performances, in which one of the best ones was found in diet group IMP-0.6, but the values did not significantly differ from those in diet groups IMP-0.4 and INO-0.4, respectively. Superoxide dismutase, peroxidase and bactericidal activity were significantly influenced by dietary Supplementation of inosine and IMP. However, the values of these parameters were not significantly different among diet groups INO-0.4, IMP-0.4 and IMP-0.6. Total serum protein, catalase and lysozyme activity were also improved (P > 0.05) by dietary Supplementation of inosine and IMP. Supplementations improved both freshwater stress and oxidative stress resistances. Fish fed diet groups INO-0.4 and IMP-0.6 showed the least oxidative stress condition. Inosine and IMP Supplementations significantly increased enterocyte height (hE), fold height (hF) of both anterior and posterior intestine and microvillus height (hMV) of posterior intestine compared to those of the control. The highest hF observed in diet group IMP-0.4 and the mean value was not significantly different from those in other IMP diets, INO-0.4 and INO-0.6, respectively. Significantly highest anterior hE and posterior hF, hE and hMV observed in diet group INO-0.4. However, no significant difference of posterior hF observed between INO-0.4 and IMP supplemented diet groups. Fish fed supplemented diets also showed improved survival compared to control (P > 0.05). Considering overall performances, it can be concluded that diet groups INO-0.4 and IMP-0.4 showed relatively better performance among inosine and IMP supplemented diet groups respectively compared to control. Furthermore, diets supplemented with either inosine or IMP at 0.4% are beneficial to promote growth, immune responses, stress resistance and intestinal health condition of juvenile red sea bream. Statement of relevance In this study inosine and inosine monophosphate has been evaluated as potential functional nutrients for marine fish such as red sea bream. Since Supplementations of such immunostimulant-like functional nutrients are very important for the development of functional aquafeeds, I hope findings of this paper will help to develop low fishmeal based functional aquafeeds in near future.
Manabu Ishikawa - One of the best experts on this subject based on the ideXlab platform.
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dietary cytidine monophosphate enhances the growth blood characteristics innate and adaptive immune functions and stress resistance of juvenile red sea bream pagrus major
Aquaculture, 2017Co-Authors: Md Sakhawat Hossain, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Nadia Mahjabin SonyAbstract:Abstract The present study was designed to evaluate the effects of dietary cytidine monophosphate on growth, blood characteristics, innate and adaptive immune functions and stress resistance of juvenile red sea bream, Pagrus major . A semi-purified basal diet supplemented with 0% (Control), 0.1% (CMP-0.1), 0.2% (CMP-0.2), 0.4% (CMP-0.4) and 0.8% (CMP-0.8) purified CMP to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (mean initial weight 2.9 g) for 56 days. In general dietary CMP Supplementations tended to improve growth performances (final body weight, % weight gain and specific growth rate), in which significantly highest performances were found in diet group CMP-0.4 followed by diet groups CMP-0.8 and CMP-0.2. CMP Supplementation resulting in increased feed conversion efficiency and protein efficiency ratio and highest value (P 0.05) with dietary CMP Supplementations. Dietary Supplementation of CMP had an increase (P > 0.05) adaptive humoral immune responses (antibody titer) after 21 days of vaccination of formalin killed Vibrio anguillarum . In terms of oxidative stress diet groups CMP-0.2 and CMP-0.4 showed best condition with low oxidative stress and high antioxidant levels. Moreover, the fish fed CMP supplemented diets had better improvement (P
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dietary cytidine monophosphate enhances the growth blood characteristics innate and adaptive immune functions and stress resistance of juvenile red sea bream pagrus major
Aquaculture, 2017Co-Authors: Md Sakhawat Hossain, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Nadia Mahjabin SonyAbstract:Abstract The present study was designed to evaluate the effects of dietary cytidine monophosphate on growth, blood characteristics, innate and adaptive immune functions and stress resistance of juvenile red sea bream, Pagrus major . A semi-purified basal diet supplemented with 0% (Control), 0.1% (CMP-0.1), 0.2% (CMP-0.2), 0.4% (CMP-0.4) and 0.8% (CMP-0.8) purified CMP to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (mean initial weight 2.9 g) for 56 days. In general dietary CMP Supplementations tended to improve growth performances (final body weight, % weight gain and specific growth rate), in which significantly highest performances were found in diet group CMP-0.4 followed by diet groups CMP-0.8 and CMP-0.2. CMP Supplementation resulting in increased feed conversion efficiency and protein efficiency ratio and highest value (P 0.05) with dietary CMP Supplementations. Dietary Supplementation of CMP had an increase (P > 0.05) adaptive humoral immune responses (antibody titer) after 21 days of vaccination of formalin killed Vibrio anguillarum . In terms of oxidative stress diet groups CMP-0.2 and CMP-0.4 showed best condition with low oxidative stress and high antioxidant levels. Moreover, the fish fed CMP supplemented diets had better improvement (P
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comparison of the effects of inosine and inosine monophosphate on growth immune response stress resistance and gut morphology of juvenile red sea bream pagrus major
Aquaculture, 2016Co-Authors: Md Sakhawat Hossain, Nadia Mahjabin Sony, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Takeshi FujiedaAbstract:Abstract Inosine and inosine monophosphate (IMP) are the most extensively studied specific nucleotide for feeding stimulation research rather than using as functional nutrients for potential growth and health benefit of aquatic species. Therefore, a 10-week feeding trial was conducted to determine comparative effects of inosine and IMP as functional nutrients on growth performance, immune responses, stress resistance and intestinal morphology of juvenile red sea bream ( Pagrus major ). Casein based semi-purified basal diet was formulated to contain 54% protein, 11% lipid, without inosine and IMP Supplementation (Control). Four levels of inosine nucleoside (0.2, 0.4, 0.6 and 0.8% for diet groups INO-0.2, INO-0.4, INO-0.6 and INO-0.8 respectively) and IMP nucleotide (0.2, 0.4, 0.6 and 0.8% for diet groups IMP-0.2, IMP-0.4, IMP-0.6 and IMP-0.8 respectively) were added to the basal diet. Each diet was randomly allocated to triplicate groups of fish with initial average weight of 6.6 g. The results indicated that dietary inosine and IMP Supplementations tended to improve growth performances, in which one of the best ones was found in diet group IMP-0.6, but the values did not significantly differ from those in diet groups IMP-0.4 and INO-0.4, respectively. Superoxide dismutase, peroxidase and bactericidal activity were significantly influenced by dietary Supplementation of inosine and IMP. However, the values of these parameters were not significantly different among diet groups INO-0.4, IMP-0.4 and IMP-0.6. Total serum protein, catalase and lysozyme activity were also improved (P > 0.05) by dietary Supplementation of inosine and IMP. Supplementations improved both freshwater stress and oxidative stress resistances. Fish fed diet groups INO-0.4 and IMP-0.6 showed the least oxidative stress condition. Inosine and IMP Supplementations significantly increased enterocyte height (hE), fold height (hF) of both anterior and posterior intestine and microvillus height (hMV) of posterior intestine compared to those of the control. The highest hF observed in diet group IMP-0.4 and the mean value was not significantly different from those in other IMP diets, INO-0.4 and INO-0.6, respectively. Significantly highest anterior hE and posterior hF, hE and hMV observed in diet group INO-0.4. However, no significant difference of posterior hF observed between INO-0.4 and IMP supplemented diet groups. Fish fed supplemented diets also showed improved survival compared to control (P > 0.05). Considering overall performances, it can be concluded that diet groups INO-0.4 and IMP-0.4 showed relatively better performance among inosine and IMP supplemented diet groups respectively compared to control. Furthermore, diets supplemented with either inosine or IMP at 0.4% are beneficial to promote growth, immune responses, stress resistance and intestinal health condition of juvenile red sea bream. Statement of relevance In this study inosine and inosine monophosphate has been evaluated as potential functional nutrients for marine fish such as red sea bream. Since Supplementations of such immunostimulant-like functional nutrients are very important for the development of functional aquafeeds, I hope findings of this paper will help to develop low fishmeal based functional aquafeeds in near future.
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interactive effects of vitamin c and e Supplementation on growth performance fatty acid composition and reduction of oxidative stress in juvenile japanese flounder paralichthys olivaceus fed dietary oxidized fish oil
Aquaculture, 2014Co-Authors: Jian Gao, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Roger Edward P MamauagAbstract:Abstract A study was conducted to determine the interactive effects of vitamin C (VC) and E (VE) Supplementation on growth, fatty acid composition and oxidative status of Japanese flounder juveniles. Fish (initial average body weight of 1.1 ± 0.1 g) in triplicate were fed five test diets for 60 days. Control diet contained fresh fish oil (FFO, 8.9 meq/kg) with 100 mg α-tocopherol (α-Toc) equivalents/kg of VE and 500 mg ascorbic acid (AsA) equivalents/kg of VC (FFO100E/500C). The other four diets contained oxidized fish oil (OFO, 167.8 meq/kg) with varying levels of VE (mg/kg) and VC (mg/kg) (OFO100E/500C, OFO200E/500C, OFO100E/1000C and OFO200E/1000C). Fish fed FFO100E/500C and OFO100E/500C had no differences in body weight gain (BWG). However, fish fed OFO200E/1000C diet had a significantly lower BWG than FFO100E/500C. Fish fed OFO200E/500C and OFO100E/1000C showed no differences in thiobarbituric acid-reactive substance values compared with FFO100E/500C. Increasing the levels of VC and VE Supplementation increased liver AsA and α-Toc contents, respectively. Liver α-Toc content was significantly increased with incremental dietary VC levels, indicating a sparing effect of VC on liver α-Toc content of fish. Increasing the levels of dietary VC and VE Supplementations decreased concentrations of 20:5n-3, 22:5n-3 and 22:6n-3 in fish liver. Fish fed OFO100E/500C and OFO200E/1000C diets showed higher oxidative stress condition than those fed FFO100E/500C. In conclusion, dietary VC and VE Supplementation could maintain normal growth and health condition of juvenile Japanese flounder fed OFO. However, high doses of both vitamin supplements induced fish lipid peroxidation under oxidative stress condition.
Saichiro Yokoyama - One of the best experts on this subject based on the ideXlab platform.
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dietary cytidine monophosphate enhances the growth blood characteristics innate and adaptive immune functions and stress resistance of juvenile red sea bream pagrus major
Aquaculture, 2017Co-Authors: Md Sakhawat Hossain, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Nadia Mahjabin SonyAbstract:Abstract The present study was designed to evaluate the effects of dietary cytidine monophosphate on growth, blood characteristics, innate and adaptive immune functions and stress resistance of juvenile red sea bream, Pagrus major . A semi-purified basal diet supplemented with 0% (Control), 0.1% (CMP-0.1), 0.2% (CMP-0.2), 0.4% (CMP-0.4) and 0.8% (CMP-0.8) purified CMP to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (mean initial weight 2.9 g) for 56 days. In general dietary CMP Supplementations tended to improve growth performances (final body weight, % weight gain and specific growth rate), in which significantly highest performances were found in diet group CMP-0.4 followed by diet groups CMP-0.8 and CMP-0.2. CMP Supplementation resulting in increased feed conversion efficiency and protein efficiency ratio and highest value (P 0.05) with dietary CMP Supplementations. Dietary Supplementation of CMP had an increase (P > 0.05) adaptive humoral immune responses (antibody titer) after 21 days of vaccination of formalin killed Vibrio anguillarum . In terms of oxidative stress diet groups CMP-0.2 and CMP-0.4 showed best condition with low oxidative stress and high antioxidant levels. Moreover, the fish fed CMP supplemented diets had better improvement (P
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dietary cytidine monophosphate enhances the growth blood characteristics innate and adaptive immune functions and stress resistance of juvenile red sea bream pagrus major
Aquaculture, 2017Co-Authors: Md Sakhawat Hossain, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Nadia Mahjabin SonyAbstract:Abstract The present study was designed to evaluate the effects of dietary cytidine monophosphate on growth, blood characteristics, innate and adaptive immune functions and stress resistance of juvenile red sea bream, Pagrus major . A semi-purified basal diet supplemented with 0% (Control), 0.1% (CMP-0.1), 0.2% (CMP-0.2), 0.4% (CMP-0.4) and 0.8% (CMP-0.8) purified CMP to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (mean initial weight 2.9 g) for 56 days. In general dietary CMP Supplementations tended to improve growth performances (final body weight, % weight gain and specific growth rate), in which significantly highest performances were found in diet group CMP-0.4 followed by diet groups CMP-0.8 and CMP-0.2. CMP Supplementation resulting in increased feed conversion efficiency and protein efficiency ratio and highest value (P 0.05) with dietary CMP Supplementations. Dietary Supplementation of CMP had an increase (P > 0.05) adaptive humoral immune responses (antibody titer) after 21 days of vaccination of formalin killed Vibrio anguillarum . In terms of oxidative stress diet groups CMP-0.2 and CMP-0.4 showed best condition with low oxidative stress and high antioxidant levels. Moreover, the fish fed CMP supplemented diets had better improvement (P
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comparison of the effects of inosine and inosine monophosphate on growth immune response stress resistance and gut morphology of juvenile red sea bream pagrus major
Aquaculture, 2016Co-Authors: Md Sakhawat Hossain, Nadia Mahjabin Sony, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Takeshi FujiedaAbstract:Abstract Inosine and inosine monophosphate (IMP) are the most extensively studied specific nucleotide for feeding stimulation research rather than using as functional nutrients for potential growth and health benefit of aquatic species. Therefore, a 10-week feeding trial was conducted to determine comparative effects of inosine and IMP as functional nutrients on growth performance, immune responses, stress resistance and intestinal morphology of juvenile red sea bream ( Pagrus major ). Casein based semi-purified basal diet was formulated to contain 54% protein, 11% lipid, without inosine and IMP Supplementation (Control). Four levels of inosine nucleoside (0.2, 0.4, 0.6 and 0.8% for diet groups INO-0.2, INO-0.4, INO-0.6 and INO-0.8 respectively) and IMP nucleotide (0.2, 0.4, 0.6 and 0.8% for diet groups IMP-0.2, IMP-0.4, IMP-0.6 and IMP-0.8 respectively) were added to the basal diet. Each diet was randomly allocated to triplicate groups of fish with initial average weight of 6.6 g. The results indicated that dietary inosine and IMP Supplementations tended to improve growth performances, in which one of the best ones was found in diet group IMP-0.6, but the values did not significantly differ from those in diet groups IMP-0.4 and INO-0.4, respectively. Superoxide dismutase, peroxidase and bactericidal activity were significantly influenced by dietary Supplementation of inosine and IMP. However, the values of these parameters were not significantly different among diet groups INO-0.4, IMP-0.4 and IMP-0.6. Total serum protein, catalase and lysozyme activity were also improved (P > 0.05) by dietary Supplementation of inosine and IMP. Supplementations improved both freshwater stress and oxidative stress resistances. Fish fed diet groups INO-0.4 and IMP-0.6 showed the least oxidative stress condition. Inosine and IMP Supplementations significantly increased enterocyte height (hE), fold height (hF) of both anterior and posterior intestine and microvillus height (hMV) of posterior intestine compared to those of the control. The highest hF observed in diet group IMP-0.4 and the mean value was not significantly different from those in other IMP diets, INO-0.4 and INO-0.6, respectively. Significantly highest anterior hE and posterior hF, hE and hMV observed in diet group INO-0.4. However, no significant difference of posterior hF observed between INO-0.4 and IMP supplemented diet groups. Fish fed supplemented diets also showed improved survival compared to control (P > 0.05). Considering overall performances, it can be concluded that diet groups INO-0.4 and IMP-0.4 showed relatively better performance among inosine and IMP supplemented diet groups respectively compared to control. Furthermore, diets supplemented with either inosine or IMP at 0.4% are beneficial to promote growth, immune responses, stress resistance and intestinal health condition of juvenile red sea bream. Statement of relevance In this study inosine and inosine monophosphate has been evaluated as potential functional nutrients for marine fish such as red sea bream. Since Supplementations of such immunostimulant-like functional nutrients are very important for the development of functional aquafeeds, I hope findings of this paper will help to develop low fishmeal based functional aquafeeds in near future.
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interactive effects of vitamin c and e Supplementation on growth performance fatty acid composition and reduction of oxidative stress in juvenile japanese flounder paralichthys olivaceus fed dietary oxidized fish oil
Aquaculture, 2014Co-Authors: Jian Gao, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Roger Edward P MamauagAbstract:Abstract A study was conducted to determine the interactive effects of vitamin C (VC) and E (VE) Supplementation on growth, fatty acid composition and oxidative status of Japanese flounder juveniles. Fish (initial average body weight of 1.1 ± 0.1 g) in triplicate were fed five test diets for 60 days. Control diet contained fresh fish oil (FFO, 8.9 meq/kg) with 100 mg α-tocopherol (α-Toc) equivalents/kg of VE and 500 mg ascorbic acid (AsA) equivalents/kg of VC (FFO100E/500C). The other four diets contained oxidized fish oil (OFO, 167.8 meq/kg) with varying levels of VE (mg/kg) and VC (mg/kg) (OFO100E/500C, OFO200E/500C, OFO100E/1000C and OFO200E/1000C). Fish fed FFO100E/500C and OFO100E/500C had no differences in body weight gain (BWG). However, fish fed OFO200E/1000C diet had a significantly lower BWG than FFO100E/500C. Fish fed OFO200E/500C and OFO100E/1000C showed no differences in thiobarbituric acid-reactive substance values compared with FFO100E/500C. Increasing the levels of VC and VE Supplementation increased liver AsA and α-Toc contents, respectively. Liver α-Toc content was significantly increased with incremental dietary VC levels, indicating a sparing effect of VC on liver α-Toc content of fish. Increasing the levels of dietary VC and VE Supplementations decreased concentrations of 20:5n-3, 22:5n-3 and 22:6n-3 in fish liver. Fish fed OFO100E/500C and OFO200E/1000C diets showed higher oxidative stress condition than those fed FFO100E/500C. In conclusion, dietary VC and VE Supplementation could maintain normal growth and health condition of juvenile Japanese flounder fed OFO. However, high doses of both vitamin supplements induced fish lipid peroxidation under oxidative stress condition.
Shunsuke Koshio - One of the best experts on this subject based on the ideXlab platform.
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dietary cytidine monophosphate enhances the growth blood characteristics innate and adaptive immune functions and stress resistance of juvenile red sea bream pagrus major
Aquaculture, 2017Co-Authors: Md Sakhawat Hossain, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Nadia Mahjabin SonyAbstract:Abstract The present study was designed to evaluate the effects of dietary cytidine monophosphate on growth, blood characteristics, innate and adaptive immune functions and stress resistance of juvenile red sea bream, Pagrus major . A semi-purified basal diet supplemented with 0% (Control), 0.1% (CMP-0.1), 0.2% (CMP-0.2), 0.4% (CMP-0.4) and 0.8% (CMP-0.8) purified CMP to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (mean initial weight 2.9 g) for 56 days. In general dietary CMP Supplementations tended to improve growth performances (final body weight, % weight gain and specific growth rate), in which significantly highest performances were found in diet group CMP-0.4 followed by diet groups CMP-0.8 and CMP-0.2. CMP Supplementation resulting in increased feed conversion efficiency and protein efficiency ratio and highest value (P 0.05) with dietary CMP Supplementations. Dietary Supplementation of CMP had an increase (P > 0.05) adaptive humoral immune responses (antibody titer) after 21 days of vaccination of formalin killed Vibrio anguillarum . In terms of oxidative stress diet groups CMP-0.2 and CMP-0.4 showed best condition with low oxidative stress and high antioxidant levels. Moreover, the fish fed CMP supplemented diets had better improvement (P
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dietary cytidine monophosphate enhances the growth blood characteristics innate and adaptive immune functions and stress resistance of juvenile red sea bream pagrus major
Aquaculture, 2017Co-Authors: Md Sakhawat Hossain, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Nadia Mahjabin SonyAbstract:Abstract The present study was designed to evaluate the effects of dietary cytidine monophosphate on growth, blood characteristics, innate and adaptive immune functions and stress resistance of juvenile red sea bream, Pagrus major . A semi-purified basal diet supplemented with 0% (Control), 0.1% (CMP-0.1), 0.2% (CMP-0.2), 0.4% (CMP-0.4) and 0.8% (CMP-0.8) purified CMP to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (mean initial weight 2.9 g) for 56 days. In general dietary CMP Supplementations tended to improve growth performances (final body weight, % weight gain and specific growth rate), in which significantly highest performances were found in diet group CMP-0.4 followed by diet groups CMP-0.8 and CMP-0.2. CMP Supplementation resulting in increased feed conversion efficiency and protein efficiency ratio and highest value (P 0.05) with dietary CMP Supplementations. Dietary Supplementation of CMP had an increase (P > 0.05) adaptive humoral immune responses (antibody titer) after 21 days of vaccination of formalin killed Vibrio anguillarum . In terms of oxidative stress diet groups CMP-0.2 and CMP-0.4 showed best condition with low oxidative stress and high antioxidant levels. Moreover, the fish fed CMP supplemented diets had better improvement (P
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comparison of the effects of inosine and inosine monophosphate on growth immune response stress resistance and gut morphology of juvenile red sea bream pagrus major
Aquaculture, 2016Co-Authors: Md Sakhawat Hossain, Nadia Mahjabin Sony, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Takeshi FujiedaAbstract:Abstract Inosine and inosine monophosphate (IMP) are the most extensively studied specific nucleotide for feeding stimulation research rather than using as functional nutrients for potential growth and health benefit of aquatic species. Therefore, a 10-week feeding trial was conducted to determine comparative effects of inosine and IMP as functional nutrients on growth performance, immune responses, stress resistance and intestinal morphology of juvenile red sea bream ( Pagrus major ). Casein based semi-purified basal diet was formulated to contain 54% protein, 11% lipid, without inosine and IMP Supplementation (Control). Four levels of inosine nucleoside (0.2, 0.4, 0.6 and 0.8% for diet groups INO-0.2, INO-0.4, INO-0.6 and INO-0.8 respectively) and IMP nucleotide (0.2, 0.4, 0.6 and 0.8% for diet groups IMP-0.2, IMP-0.4, IMP-0.6 and IMP-0.8 respectively) were added to the basal diet. Each diet was randomly allocated to triplicate groups of fish with initial average weight of 6.6 g. The results indicated that dietary inosine and IMP Supplementations tended to improve growth performances, in which one of the best ones was found in diet group IMP-0.6, but the values did not significantly differ from those in diet groups IMP-0.4 and INO-0.4, respectively. Superoxide dismutase, peroxidase and bactericidal activity were significantly influenced by dietary Supplementation of inosine and IMP. However, the values of these parameters were not significantly different among diet groups INO-0.4, IMP-0.4 and IMP-0.6. Total serum protein, catalase and lysozyme activity were also improved (P > 0.05) by dietary Supplementation of inosine and IMP. Supplementations improved both freshwater stress and oxidative stress resistances. Fish fed diet groups INO-0.4 and IMP-0.6 showed the least oxidative stress condition. Inosine and IMP Supplementations significantly increased enterocyte height (hE), fold height (hF) of both anterior and posterior intestine and microvillus height (hMV) of posterior intestine compared to those of the control. The highest hF observed in diet group IMP-0.4 and the mean value was not significantly different from those in other IMP diets, INO-0.4 and INO-0.6, respectively. Significantly highest anterior hE and posterior hF, hE and hMV observed in diet group INO-0.4. However, no significant difference of posterior hF observed between INO-0.4 and IMP supplemented diet groups. Fish fed supplemented diets also showed improved survival compared to control (P > 0.05). Considering overall performances, it can be concluded that diet groups INO-0.4 and IMP-0.4 showed relatively better performance among inosine and IMP supplemented diet groups respectively compared to control. Furthermore, diets supplemented with either inosine or IMP at 0.4% are beneficial to promote growth, immune responses, stress resistance and intestinal health condition of juvenile red sea bream. Statement of relevance In this study inosine and inosine monophosphate has been evaluated as potential functional nutrients for marine fish such as red sea bream. Since Supplementations of such immunostimulant-like functional nutrients are very important for the development of functional aquafeeds, I hope findings of this paper will help to develop low fishmeal based functional aquafeeds in near future.
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interactive effects of vitamin c and e Supplementation on growth performance fatty acid composition and reduction of oxidative stress in juvenile japanese flounder paralichthys olivaceus fed dietary oxidized fish oil
Aquaculture, 2014Co-Authors: Jian Gao, Saichiro Yokoyama, Shunsuke Koshio, Manabu Ishikawa, Roger Edward P MamauagAbstract:Abstract A study was conducted to determine the interactive effects of vitamin C (VC) and E (VE) Supplementation on growth, fatty acid composition and oxidative status of Japanese flounder juveniles. Fish (initial average body weight of 1.1 ± 0.1 g) in triplicate were fed five test diets for 60 days. Control diet contained fresh fish oil (FFO, 8.9 meq/kg) with 100 mg α-tocopherol (α-Toc) equivalents/kg of VE and 500 mg ascorbic acid (AsA) equivalents/kg of VC (FFO100E/500C). The other four diets contained oxidized fish oil (OFO, 167.8 meq/kg) with varying levels of VE (mg/kg) and VC (mg/kg) (OFO100E/500C, OFO200E/500C, OFO100E/1000C and OFO200E/1000C). Fish fed FFO100E/500C and OFO100E/500C had no differences in body weight gain (BWG). However, fish fed OFO200E/1000C diet had a significantly lower BWG than FFO100E/500C. Fish fed OFO200E/500C and OFO100E/1000C showed no differences in thiobarbituric acid-reactive substance values compared with FFO100E/500C. Increasing the levels of VC and VE Supplementation increased liver AsA and α-Toc contents, respectively. Liver α-Toc content was significantly increased with incremental dietary VC levels, indicating a sparing effect of VC on liver α-Toc content of fish. Increasing the levels of dietary VC and VE Supplementations decreased concentrations of 20:5n-3, 22:5n-3 and 22:6n-3 in fish liver. Fish fed OFO100E/500C and OFO200E/1000C diets showed higher oxidative stress condition than those fed FFO100E/500C. In conclusion, dietary VC and VE Supplementation could maintain normal growth and health condition of juvenile Japanese flounder fed OFO. However, high doses of both vitamin supplements induced fish lipid peroxidation under oxidative stress condition.
