The Experts below are selected from a list of 300 Experts worldwide ranked by ideXlab platform
David R Weaver - One of the best experts on this subject based on the ideXlab platform.
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expression of basic helix loop helix pas genes in the mouse Suprachiasmatic Nucleus
Neuroscience, 1999Co-Authors: Lauren P Shearman, Mark J Zylka, Steven M Reppert, David R WeaverAbstract:The Suprachiasmatic nuclei contain a circadian clock that drives rhythmicity in physiology and behavior. In mice, mutation of theClock gene produces abnormal circadian behavior [Vitaterna M. H.et al. (1994)Science264, 715–725]. TheClock gene encodes a protein containing basic helix-loop-helix and PAS (PER-ARNT-SIM) domains [King D. P.et al. (1997)Cell89, 641–653]. The PAS domain may be an important structural feature of a subset of genes involved in photoreception and circadian rhythmicity. The expression and regulation of messenger RNAs encoding eight members of the basic helix-loop-helix/PAS protein superfamily were examined byin situ hybridization. Six of the genes studied (aryl hydrocarbon receptor nuclear transporter, aryl hydrocarbon receptor nuclear transporter-2,Clock, endothelial PAS-containing protein, hypoxia-inducible factor1α and steroid receptor coactivator-1) were expressed in the Suprachiasmatic Nucleus of adult and neonatal mice. No evidence for rhythmicity of expression was observed when comparing brains collected early in the subjective day (circadian time 3) with those collected early in subjective night (circadian time 15). Neuronal PAS-containing protein-1 messenger RNA was expressed in the Suprachiasmatic Nucleus of adult (but not neonatal) mice, and a low-amplitude rhythm of neuronal PAS-containing protein-1 gene expression was detected in the Suprachiasmatic Nucleus. Neuronal PAS-containing protein-2 messenger RNA was not detected in adult or neonatal Suprachiasmatic Nucleus. Exposure to light at night (30 or 180 min of light, beginning at circadian time 15) did not alter the expression of any of the genes studied. The expression of multiple members of the basic helix-loop-helix/PAS family in the Suprachiasmatic Nucleus suggests a rich array of potential interactions relevant to the regulation of the Suprachiasmatic circadian clock.
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Expression of basic helix-loop-helix/PAS genes in the mouse Suprachiasmatic Nucleus
Neuroscience, 1999Co-Authors: Lauren P Shearman, Mark J Zylka, Steven M Reppert, David R WeaverAbstract:The Suprachiasmatic nuclei contain a circadian clock that drives rhythmicity in physiology and behavior. In mice, mutation of theClock gene produces abnormal circadian behavior [Vitaterna M. H.et al. (1994)Science264, 715–725]. TheClock gene encodes a protein containing basic helix-loop-helix and PAS (PER-ARNT-SIM) domains [King D. P.et al. (1997)Cell89, 641–653]. The PAS domain may be an important structural feature of a subset of genes involved in photoreception and circadian rhythmicity. The expression and regulation of messenger RNAs encoding eight members of the basic helix-loop-helix/PAS protein superfamily were examined byin situ hybridization. Six of the genes studied (aryl hydrocarbon receptor nuclear transporter, aryl hydrocarbon receptor nuclear transporter-2,Clock, endothelial PAS-containing protein, hypoxia-inducible factor1α and steroid receptor coactivator-1) were expressed in the Suprachiasmatic Nucleus of adult and neonatal mice. No evidence for rhythmicity of expression was observed when comparing brains collected early in the subjective day (circadian time 3) with those collected early in subjective night (circadian time 15). Neuronal PAS-containing protein-1 messenger RNA was expressed in the Suprachiasmatic Nucleus of adult (but not neonatal) mice, and a low-amplitude rhythm of neuronal PAS-containing protein-1 gene expression was detected in the Suprachiasmatic Nucleus. Neuronal PAS-containing protein-2 messenger RNA was not detected in adult or neonatal Suprachiasmatic Nucleus. Exposure to light at night (30 or 180 min of light, beginning at circadian time 15) did not alter the expression of any of the genes studied. The expression of multiple members of the basic helix-loop-helix/PAS family in the Suprachiasmatic Nucleus suggests a rich array of potential interactions relevant to the regulation of the Suprachiasmatic circadian clock.
Lauren P Shearman - One of the best experts on this subject based on the ideXlab platform.
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expression of basic helix loop helix pas genes in the mouse Suprachiasmatic Nucleus
Neuroscience, 1999Co-Authors: Lauren P Shearman, Mark J Zylka, Steven M Reppert, David R WeaverAbstract:The Suprachiasmatic nuclei contain a circadian clock that drives rhythmicity in physiology and behavior. In mice, mutation of theClock gene produces abnormal circadian behavior [Vitaterna M. H.et al. (1994)Science264, 715–725]. TheClock gene encodes a protein containing basic helix-loop-helix and PAS (PER-ARNT-SIM) domains [King D. P.et al. (1997)Cell89, 641–653]. The PAS domain may be an important structural feature of a subset of genes involved in photoreception and circadian rhythmicity. The expression and regulation of messenger RNAs encoding eight members of the basic helix-loop-helix/PAS protein superfamily were examined byin situ hybridization. Six of the genes studied (aryl hydrocarbon receptor nuclear transporter, aryl hydrocarbon receptor nuclear transporter-2,Clock, endothelial PAS-containing protein, hypoxia-inducible factor1α and steroid receptor coactivator-1) were expressed in the Suprachiasmatic Nucleus of adult and neonatal mice. No evidence for rhythmicity of expression was observed when comparing brains collected early in the subjective day (circadian time 3) with those collected early in subjective night (circadian time 15). Neuronal PAS-containing protein-1 messenger RNA was expressed in the Suprachiasmatic Nucleus of adult (but not neonatal) mice, and a low-amplitude rhythm of neuronal PAS-containing protein-1 gene expression was detected in the Suprachiasmatic Nucleus. Neuronal PAS-containing protein-2 messenger RNA was not detected in adult or neonatal Suprachiasmatic Nucleus. Exposure to light at night (30 or 180 min of light, beginning at circadian time 15) did not alter the expression of any of the genes studied. The expression of multiple members of the basic helix-loop-helix/PAS family in the Suprachiasmatic Nucleus suggests a rich array of potential interactions relevant to the regulation of the Suprachiasmatic circadian clock.
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Expression of basic helix-loop-helix/PAS genes in the mouse Suprachiasmatic Nucleus
Neuroscience, 1999Co-Authors: Lauren P Shearman, Mark J Zylka, Steven M Reppert, David R WeaverAbstract:The Suprachiasmatic nuclei contain a circadian clock that drives rhythmicity in physiology and behavior. In mice, mutation of theClock gene produces abnormal circadian behavior [Vitaterna M. H.et al. (1994)Science264, 715–725]. TheClock gene encodes a protein containing basic helix-loop-helix and PAS (PER-ARNT-SIM) domains [King D. P.et al. (1997)Cell89, 641–653]. The PAS domain may be an important structural feature of a subset of genes involved in photoreception and circadian rhythmicity. The expression and regulation of messenger RNAs encoding eight members of the basic helix-loop-helix/PAS protein superfamily were examined byin situ hybridization. Six of the genes studied (aryl hydrocarbon receptor nuclear transporter, aryl hydrocarbon receptor nuclear transporter-2,Clock, endothelial PAS-containing protein, hypoxia-inducible factor1α and steroid receptor coactivator-1) were expressed in the Suprachiasmatic Nucleus of adult and neonatal mice. No evidence for rhythmicity of expression was observed when comparing brains collected early in the subjective day (circadian time 3) with those collected early in subjective night (circadian time 15). Neuronal PAS-containing protein-1 messenger RNA was expressed in the Suprachiasmatic Nucleus of adult (but not neonatal) mice, and a low-amplitude rhythm of neuronal PAS-containing protein-1 gene expression was detected in the Suprachiasmatic Nucleus. Neuronal PAS-containing protein-2 messenger RNA was not detected in adult or neonatal Suprachiasmatic Nucleus. Exposure to light at night (30 or 180 min of light, beginning at circadian time 15) did not alter the expression of any of the genes studied. The expression of multiple members of the basic helix-loop-helix/PAS family in the Suprachiasmatic Nucleus suggests a rich array of potential interactions relevant to the regulation of the Suprachiasmatic circadian clock.
Gareth Leng - One of the best experts on this subject based on the ideXlab platform.
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vasopressin casts light on the Suprachiasmatic Nucleus
The Journal of Physiology, 2017Co-Authors: Takahiro Tsuji, Andrew Allchorne, Meng Zhang, Chiharu Tsuji, Vicky A Tobin, Rafael Pineda, Androniki Raftogianni, Javier Eduardo Stern, Valery Grinevich, Gareth LengAbstract:Key points A subpopulation of retinal ganglion cells expresses the neuropeptide vasopressin. These retinal ganglion cells project predominately to our biological clock, the Suprachiasmatic Nucleus (SCN). Light-induced vasopressin release enhances the responses of SCN neurons to light. It also enhances expression of genes involved in photo-entrainment of biological rhythms. Abstract In all animals, the transition between night and day engages a host of physiological and behavioural rhythms. These rhythms depend not on the rods and cones of the retina, but on retinal ganglion cells (RGCs) that detect the ambient light level in the environment. These project to the Suprachiasmatic Nucleus (SCN) of the hypothalamus to entrain circadian rhythms that are generated within the SCN. The neuropeptide vasopressin has an important role in this entrainment. Many SCN neurons express vasopressin, and it has been assumed that the role of vasopressin in the SCN reflects the activity of these cells. Here we show that vasopressin is also expressed in many retinal cells that project to the SCN. Light-evoked vasopressin release contributes to the responses of SCN neurons to light, and enhances expression of the immediate early gene c-fos in the SCN, which is involved in photic entrainment of circadian rhythms.
Mark J Zylka - One of the best experts on this subject based on the ideXlab platform.
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expression of basic helix loop helix pas genes in the mouse Suprachiasmatic Nucleus
Neuroscience, 1999Co-Authors: Lauren P Shearman, Mark J Zylka, Steven M Reppert, David R WeaverAbstract:The Suprachiasmatic nuclei contain a circadian clock that drives rhythmicity in physiology and behavior. In mice, mutation of theClock gene produces abnormal circadian behavior [Vitaterna M. H.et al. (1994)Science264, 715–725]. TheClock gene encodes a protein containing basic helix-loop-helix and PAS (PER-ARNT-SIM) domains [King D. P.et al. (1997)Cell89, 641–653]. The PAS domain may be an important structural feature of a subset of genes involved in photoreception and circadian rhythmicity. The expression and regulation of messenger RNAs encoding eight members of the basic helix-loop-helix/PAS protein superfamily were examined byin situ hybridization. Six of the genes studied (aryl hydrocarbon receptor nuclear transporter, aryl hydrocarbon receptor nuclear transporter-2,Clock, endothelial PAS-containing protein, hypoxia-inducible factor1α and steroid receptor coactivator-1) were expressed in the Suprachiasmatic Nucleus of adult and neonatal mice. No evidence for rhythmicity of expression was observed when comparing brains collected early in the subjective day (circadian time 3) with those collected early in subjective night (circadian time 15). Neuronal PAS-containing protein-1 messenger RNA was expressed in the Suprachiasmatic Nucleus of adult (but not neonatal) mice, and a low-amplitude rhythm of neuronal PAS-containing protein-1 gene expression was detected in the Suprachiasmatic Nucleus. Neuronal PAS-containing protein-2 messenger RNA was not detected in adult or neonatal Suprachiasmatic Nucleus. Exposure to light at night (30 or 180 min of light, beginning at circadian time 15) did not alter the expression of any of the genes studied. The expression of multiple members of the basic helix-loop-helix/PAS family in the Suprachiasmatic Nucleus suggests a rich array of potential interactions relevant to the regulation of the Suprachiasmatic circadian clock.
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Expression of basic helix-loop-helix/PAS genes in the mouse Suprachiasmatic Nucleus
Neuroscience, 1999Co-Authors: Lauren P Shearman, Mark J Zylka, Steven M Reppert, David R WeaverAbstract:The Suprachiasmatic nuclei contain a circadian clock that drives rhythmicity in physiology and behavior. In mice, mutation of theClock gene produces abnormal circadian behavior [Vitaterna M. H.et al. (1994)Science264, 715–725]. TheClock gene encodes a protein containing basic helix-loop-helix and PAS (PER-ARNT-SIM) domains [King D. P.et al. (1997)Cell89, 641–653]. The PAS domain may be an important structural feature of a subset of genes involved in photoreception and circadian rhythmicity. The expression and regulation of messenger RNAs encoding eight members of the basic helix-loop-helix/PAS protein superfamily were examined byin situ hybridization. Six of the genes studied (aryl hydrocarbon receptor nuclear transporter, aryl hydrocarbon receptor nuclear transporter-2,Clock, endothelial PAS-containing protein, hypoxia-inducible factor1α and steroid receptor coactivator-1) were expressed in the Suprachiasmatic Nucleus of adult and neonatal mice. No evidence for rhythmicity of expression was observed when comparing brains collected early in the subjective day (circadian time 3) with those collected early in subjective night (circadian time 15). Neuronal PAS-containing protein-1 messenger RNA was expressed in the Suprachiasmatic Nucleus of adult (but not neonatal) mice, and a low-amplitude rhythm of neuronal PAS-containing protein-1 gene expression was detected in the Suprachiasmatic Nucleus. Neuronal PAS-containing protein-2 messenger RNA was not detected in adult or neonatal Suprachiasmatic Nucleus. Exposure to light at night (30 or 180 min of light, beginning at circadian time 15) did not alter the expression of any of the genes studied. The expression of multiple members of the basic helix-loop-helix/PAS family in the Suprachiasmatic Nucleus suggests a rich array of potential interactions relevant to the regulation of the Suprachiasmatic circadian clock.
Steven M Reppert - One of the best experts on this subject based on the ideXlab platform.
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expression of basic helix loop helix pas genes in the mouse Suprachiasmatic Nucleus
Neuroscience, 1999Co-Authors: Lauren P Shearman, Mark J Zylka, Steven M Reppert, David R WeaverAbstract:The Suprachiasmatic nuclei contain a circadian clock that drives rhythmicity in physiology and behavior. In mice, mutation of theClock gene produces abnormal circadian behavior [Vitaterna M. H.et al. (1994)Science264, 715–725]. TheClock gene encodes a protein containing basic helix-loop-helix and PAS (PER-ARNT-SIM) domains [King D. P.et al. (1997)Cell89, 641–653]. The PAS domain may be an important structural feature of a subset of genes involved in photoreception and circadian rhythmicity. The expression and regulation of messenger RNAs encoding eight members of the basic helix-loop-helix/PAS protein superfamily were examined byin situ hybridization. Six of the genes studied (aryl hydrocarbon receptor nuclear transporter, aryl hydrocarbon receptor nuclear transporter-2,Clock, endothelial PAS-containing protein, hypoxia-inducible factor1α and steroid receptor coactivator-1) were expressed in the Suprachiasmatic Nucleus of adult and neonatal mice. No evidence for rhythmicity of expression was observed when comparing brains collected early in the subjective day (circadian time 3) with those collected early in subjective night (circadian time 15). Neuronal PAS-containing protein-1 messenger RNA was expressed in the Suprachiasmatic Nucleus of adult (but not neonatal) mice, and a low-amplitude rhythm of neuronal PAS-containing protein-1 gene expression was detected in the Suprachiasmatic Nucleus. Neuronal PAS-containing protein-2 messenger RNA was not detected in adult or neonatal Suprachiasmatic Nucleus. Exposure to light at night (30 or 180 min of light, beginning at circadian time 15) did not alter the expression of any of the genes studied. The expression of multiple members of the basic helix-loop-helix/PAS family in the Suprachiasmatic Nucleus suggests a rich array of potential interactions relevant to the regulation of the Suprachiasmatic circadian clock.
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Expression of basic helix-loop-helix/PAS genes in the mouse Suprachiasmatic Nucleus
Neuroscience, 1999Co-Authors: Lauren P Shearman, Mark J Zylka, Steven M Reppert, David R WeaverAbstract:The Suprachiasmatic nuclei contain a circadian clock that drives rhythmicity in physiology and behavior. In mice, mutation of theClock gene produces abnormal circadian behavior [Vitaterna M. H.et al. (1994)Science264, 715–725]. TheClock gene encodes a protein containing basic helix-loop-helix and PAS (PER-ARNT-SIM) domains [King D. P.et al. (1997)Cell89, 641–653]. The PAS domain may be an important structural feature of a subset of genes involved in photoreception and circadian rhythmicity. The expression and regulation of messenger RNAs encoding eight members of the basic helix-loop-helix/PAS protein superfamily were examined byin situ hybridization. Six of the genes studied (aryl hydrocarbon receptor nuclear transporter, aryl hydrocarbon receptor nuclear transporter-2,Clock, endothelial PAS-containing protein, hypoxia-inducible factor1α and steroid receptor coactivator-1) were expressed in the Suprachiasmatic Nucleus of adult and neonatal mice. No evidence for rhythmicity of expression was observed when comparing brains collected early in the subjective day (circadian time 3) with those collected early in subjective night (circadian time 15). Neuronal PAS-containing protein-1 messenger RNA was expressed in the Suprachiasmatic Nucleus of adult (but not neonatal) mice, and a low-amplitude rhythm of neuronal PAS-containing protein-1 gene expression was detected in the Suprachiasmatic Nucleus. Neuronal PAS-containing protein-2 messenger RNA was not detected in adult or neonatal Suprachiasmatic Nucleus. Exposure to light at night (30 or 180 min of light, beginning at circadian time 15) did not alter the expression of any of the genes studied. The expression of multiple members of the basic helix-loop-helix/PAS family in the Suprachiasmatic Nucleus suggests a rich array of potential interactions relevant to the regulation of the Suprachiasmatic circadian clock.
