The Experts below are selected from a list of 48168 Experts worldwide ranked by ideXlab platform
Manuela E. Gomes - One of the best experts on this subject based on the ideXlab platform.
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tropoelastin coated tendon biomimetic scaffolds promote stem cell tenogenic commitment and deposition of elastin rich matrix
ACS Applied Materials & Interfaces, 2019Co-Authors: Helena Almeida, Rui M. A. Domingues, Suzanne M. Mithieux, Ricardo A. Pires, Rui L. Reis, Anthony S. Weiss, Ana I Goncalves, Manuel Gomezflorit, Manuela E. GomesAbstract:Tendon tissue engineering strategies that recreate the biophysical and biochemical native microenvironment have a greater potential to achieve regeneration. Here, we developed tendon biomimetic scaffolds using mechanically competent yarns of poly-e-caprolactone, chitosan, and cellulose nanocrystals to recreate the inherent tendon hierarchy from a nano-to-macro scale. These were then coated with tropoelastin (TROPO) through polydopamine (PDA) linking, to mimic the native extracellular matrix (ECM) composition and elasticity. Both PDA and TROPO coatings decreased Surface Stiffness without masking the underlying substrate. We found that human adipose-derived stem cells (hASCs) seeded onto these TROPO biomimetic scaffolds more rapidly acquired their spindle-shape morphology and high aspect ratio characteristic of tenocytes. Immunocytochemistry shows that the PDA and TROPO-coated Surfaces boosted differentiation of hASCs toward the tenogenic lineage, with sustained expression of the tendon-related markers scleraxis and tenomodulin up to 21 days of culture. Furthermore, these Surfaces enabled the deposition of a tendon-like ECM, supported by the expression of collagens type I and III, tenascin, and decorin. Gene expression analysis revealed a downregulation of osteogenic and fibrosis markers in the presence of TROPO when compared with the control groups, suggesting proper ECM deposition. Remarkably, differentiated cells exposed to TROPO acquired an elastogenic profile due to the evident elastin synthesis and deposition, contributing to the formation of a more mimetic matrix in comparison with the PDA-coated and uncoated conditions. In summary, our biomimetic substrates combining biophysical and biological cues modulate stem cell behavior potentiating their long-term tenogenic commitment and the production of an elastin-rich ECM.
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Tropoelastin-Coated Tendon Biomimetic Scaffolds Promote Stem Cell Tenogenic Commitment and Deposition of Elastin-Rich Matrix
2019Co-Authors: Helena Almeida, Rui M. A. Domingues, Suzanne M. Mithieux, Ricardo A. Pires, Ana I. Gonçalves, Manuel Gómez-florit, Rui L. Reis, Anthony S. Weiss, Manuela E. GomesAbstract:Tendon tissue engineering strategies that recreate the biophysical and biochemical native microenvironment have a greater potential to achieve regeneration. Here, we developed tendon biomimetic scaffolds using mechanically competent yarns of poly-ε-caprolactone, chitosan, and cellulose nanocrystals to recreate the inherent tendon hierarchy from a nano-to-macro scale. These were then coated with tropoelastin (TROPO) through polydopamine (PDA) linking, to mimic the native extracellular matrix (ECM) composition and elasticity. Both PDA and TROPO coatings decreased Surface Stiffness without masking the underlying substrate. We found that human adipose-derived stem cells (hASCs) seeded onto these TROPO biomimetic scaffolds more rapidly acquired their spindle-shape morphology and high aspect ratio characteristic of tenocytes. Immunocytochemistry shows that the PDA and TROPO-coated Surfaces boosted differentiation of hASCs toward the tenogenic lineage, with sustained expression of the tendon-related markers scleraxis and tenomodulin up to 21 days of culture. Furthermore, these Surfaces enabled the deposition of a tendon-like ECM, supported by the expression of collagens type I and III, tenascin, and decorin. Gene expression analysis revealed a downregulation of osteogenic and fibrosis markers in the presence of TROPO when compared with the control groups, suggesting proper ECM deposition. Remarkably, differentiated cells exposed to TROPO acquired an elastogenic profile due to the evident elastin synthesis and deposition, contributing to the formation of a more mimetic matrix in comparison with the PDA-coated and uncoated conditions. In summary, our biomimetic substrates combining biophysical and biological cues modulate stem cell behavior potentiating their long-term tenogenic commitment and the production of an elastin-rich ECM
Helena Almeida - One of the best experts on this subject based on the ideXlab platform.
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tropoelastin coated tendon biomimetic scaffolds promote stem cell tenogenic commitment and deposition of elastin rich matrix
ACS Applied Materials & Interfaces, 2019Co-Authors: Helena Almeida, Rui M. A. Domingues, Suzanne M. Mithieux, Ricardo A. Pires, Rui L. Reis, Anthony S. Weiss, Ana I Goncalves, Manuel Gomezflorit, Manuela E. GomesAbstract:Tendon tissue engineering strategies that recreate the biophysical and biochemical native microenvironment have a greater potential to achieve regeneration. Here, we developed tendon biomimetic scaffolds using mechanically competent yarns of poly-e-caprolactone, chitosan, and cellulose nanocrystals to recreate the inherent tendon hierarchy from a nano-to-macro scale. These were then coated with tropoelastin (TROPO) through polydopamine (PDA) linking, to mimic the native extracellular matrix (ECM) composition and elasticity. Both PDA and TROPO coatings decreased Surface Stiffness without masking the underlying substrate. We found that human adipose-derived stem cells (hASCs) seeded onto these TROPO biomimetic scaffolds more rapidly acquired their spindle-shape morphology and high aspect ratio characteristic of tenocytes. Immunocytochemistry shows that the PDA and TROPO-coated Surfaces boosted differentiation of hASCs toward the tenogenic lineage, with sustained expression of the tendon-related markers scleraxis and tenomodulin up to 21 days of culture. Furthermore, these Surfaces enabled the deposition of a tendon-like ECM, supported by the expression of collagens type I and III, tenascin, and decorin. Gene expression analysis revealed a downregulation of osteogenic and fibrosis markers in the presence of TROPO when compared with the control groups, suggesting proper ECM deposition. Remarkably, differentiated cells exposed to TROPO acquired an elastogenic profile due to the evident elastin synthesis and deposition, contributing to the formation of a more mimetic matrix in comparison with the PDA-coated and uncoated conditions. In summary, our biomimetic substrates combining biophysical and biological cues modulate stem cell behavior potentiating their long-term tenogenic commitment and the production of an elastin-rich ECM.
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Tropoelastin-Coated Tendon Biomimetic Scaffolds Promote Stem Cell Tenogenic Commitment and Deposition of Elastin-Rich Matrix
2019Co-Authors: Helena Almeida, Rui M. A. Domingues, Suzanne M. Mithieux, Ricardo A. Pires, Ana I. Gonçalves, Manuel Gómez-florit, Rui L. Reis, Anthony S. Weiss, Manuela E. GomesAbstract:Tendon tissue engineering strategies that recreate the biophysical and biochemical native microenvironment have a greater potential to achieve regeneration. Here, we developed tendon biomimetic scaffolds using mechanically competent yarns of poly-ε-caprolactone, chitosan, and cellulose nanocrystals to recreate the inherent tendon hierarchy from a nano-to-macro scale. These were then coated with tropoelastin (TROPO) through polydopamine (PDA) linking, to mimic the native extracellular matrix (ECM) composition and elasticity. Both PDA and TROPO coatings decreased Surface Stiffness without masking the underlying substrate. We found that human adipose-derived stem cells (hASCs) seeded onto these TROPO biomimetic scaffolds more rapidly acquired their spindle-shape morphology and high aspect ratio characteristic of tenocytes. Immunocytochemistry shows that the PDA and TROPO-coated Surfaces boosted differentiation of hASCs toward the tenogenic lineage, with sustained expression of the tendon-related markers scleraxis and tenomodulin up to 21 days of culture. Furthermore, these Surfaces enabled the deposition of a tendon-like ECM, supported by the expression of collagens type I and III, tenascin, and decorin. Gene expression analysis revealed a downregulation of osteogenic and fibrosis markers in the presence of TROPO when compared with the control groups, suggesting proper ECM deposition. Remarkably, differentiated cells exposed to TROPO acquired an elastogenic profile due to the evident elastin synthesis and deposition, contributing to the formation of a more mimetic matrix in comparison with the PDA-coated and uncoated conditions. In summary, our biomimetic substrates combining biophysical and biological cues modulate stem cell behavior potentiating their long-term tenogenic commitment and the production of an elastin-rich ECM
Joel Puibasset - One of the best experts on this subject based on the ideXlab platform.
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elastic compliance and Stiffness matrix of the fcc lennard jones thin films influence of thickness and temperature
The Journal of Physical Chemistry, 2019Co-Authors: Joel PuibassetAbstract:The face-centered cubic (fcc) Lennard-Jones crystal is used as a generic model of a solid to study the elastic properties of thin films as a function of thickness and temperature. The Monte Carlo algorithm is used to calculate the average deformations along the axes in the isostress–isothermal ensemble that mimics a real uniaxial loading experiment. Four independent parameters (tetragonal symmetry without shear) have been calculated for film thicknesses ranging from 4 to 12 atomic layers and for five reduced temperatures between 0 and 0.5 e/kB, where e is the energetic parameter of the Lennard-Jones potential and kB is Boltzmann’s constant. These parameters (Poisson’s ratio and moduli) give the compliance matrix, which is inverted to obtain the Stiffness coefficients. It is shown that the three Poisson’s ratios exhibit a good linearity with the inverse of the film thickness, while this is not the case for the moduli and the compliance coefficients. Remarkably, the Stiffness coefficients do exhibit a good linearity with the inverse of the film thickness, including the limiting value of infinite thickness (bulk solid) obtained by applying periodic boundary conditions in all directions. This linearity suggests to interpret the results in terms of a bulk + Surface decomposition. However, the Surface Stiffness matrix deduced from the slopes has nonzero components along the out-of-plane direction—an unexpected observation in the framework of the Surface stress theory.
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elastic compliance and Stiffness matrix of the fcc lennard jones thin films influence of thickness and temperature
arXiv: Mesoscale and Nanoscale Physics, 2019Co-Authors: Joel PuibassetAbstract:The fcc Lennard-Jones crystal is used as a generic model of solid to study the elastic properties of thin films as a function of thickness and temperature. The Monte Carlo algorithm is used to calculate the average deformations along the axes in the isostress-isothermal ensemble that mimics a real uniaxial loading experiment. The four independent parameters (tetragonal symmetry The fcc Lennard-Jones crystal is used as a generic model of solid to study the elastic properties of thin films as a function of thickness and temperature. The Monte Carlo algorithm is used to calculate the average deformations along the axes in the isostress-isothermal ensemble that mimics a real uniaxial loading experiment. The four independent parameters (tetragonal symmetry without shear) have been calculated for film thicknesses ranging from 4 to 12 atomic layers, and for five reduced temperatures between 0 and 0.5 sigma/kB, where sigma is the energetic parameter of the Lennard Jones potential and kB is Boltzmann's constant. These parameters (Poisson's ratio and moduli) give the compliance matrix, which is inverted to get the Stiffness coefficients. It is shown that the three Poisson's ratios exhibit a good linearity with the inverse of the film thickness, while this is not the case for the moduli and the compliance coefficients. Remarkably, the Stiffness coefficients do exhibit a good linearity with the inverse of the film thickness, including the limiting value of infinite thickness (bulk solid) obtained by applying periodic boundary conditions in all directions. This linearity suggests to interpret the results in terms of a bulk+Surface decomposition. However, the Surface Stiffness matrix deduced from the slopes has nonzero components along the out-of-plane direction, an unexpected observation in the framework of the Surface stress theory.
Michael Goldfarb - One of the best experts on this subject based on the ideXlab platform.
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the effect of virtual Surface Stiffness on the haptic perception of detail
IEEE-ASME Transactions on Mechatronics, 2004Co-Authors: Marcia K Omalley, Michael GoldfarbAbstract:This brief presents a quantitative study of the effects of virtual Surface Stiffness in a simulated haptic environment on the haptic perception of detail. Specifically, the haptic perception of detail is characterized by identification, detection, and discrimination of round and square cross section ridges. Test results indicate that performance, measured as a percent correct score in the perception experiments, improves in a nonlinear fashion as the maximum level of virtual Surface Stiffness in the simulation increases. Further, test subjects appeared to reach a limit in their perception capabilities at maximum Stiffness levels of 300 to 400 N/m, while the hardware was capable of 1000 N/m of maximum virtual Surface Stiffness. These results indicate that haptic interface hardware may be able to convey sufficient perceptual information to the user with relatively low levels of virtual Surface Stiffness.
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the implications of Surface Stiffness for size identification and perceived Surface hardness in haptic interfaces
International Conference on Robotics and Automation, 2002Co-Authors: Marcia K Omalley, Michael GoldfarbAbstract:This paper presents a two-part study of the effects of virtual Surface Stiffness on haptic perception. First, size identification experiments were performed to determine the effects of system quality, in terms of Surface Stiffness, on the ability of a human to identify square cross-section ridges by size in a simulated environment. Then, discrimination experiments were performed to determine relationships between virtual Surface Stiffness and simulation quality in terms of perceived Surface hardness. Results of experiments to test human haptic perception for varying virtual Surface Stiffnesses indicate that haptic interface hardware may be able to convey sufficient perceptual information to the user at relatively low levels of virtual Surface Stiffness. Subjects, however, can perceive improvements in perceived simulated Surface hardness as Stiffness levels are increased in the range of achievable parameters for this hardware. The authors draw several conclusions about the allowable time delays in a haptic interface system based on the results of the Surface Stiffness experiments.
Ricardo A. Pires - One of the best experts on this subject based on the ideXlab platform.
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tropoelastin coated tendon biomimetic scaffolds promote stem cell tenogenic commitment and deposition of elastin rich matrix
ACS Applied Materials & Interfaces, 2019Co-Authors: Helena Almeida, Rui M. A. Domingues, Suzanne M. Mithieux, Ricardo A. Pires, Rui L. Reis, Anthony S. Weiss, Ana I Goncalves, Manuel Gomezflorit, Manuela E. GomesAbstract:Tendon tissue engineering strategies that recreate the biophysical and biochemical native microenvironment have a greater potential to achieve regeneration. Here, we developed tendon biomimetic scaffolds using mechanically competent yarns of poly-e-caprolactone, chitosan, and cellulose nanocrystals to recreate the inherent tendon hierarchy from a nano-to-macro scale. These were then coated with tropoelastin (TROPO) through polydopamine (PDA) linking, to mimic the native extracellular matrix (ECM) composition and elasticity. Both PDA and TROPO coatings decreased Surface Stiffness without masking the underlying substrate. We found that human adipose-derived stem cells (hASCs) seeded onto these TROPO biomimetic scaffolds more rapidly acquired their spindle-shape morphology and high aspect ratio characteristic of tenocytes. Immunocytochemistry shows that the PDA and TROPO-coated Surfaces boosted differentiation of hASCs toward the tenogenic lineage, with sustained expression of the tendon-related markers scleraxis and tenomodulin up to 21 days of culture. Furthermore, these Surfaces enabled the deposition of a tendon-like ECM, supported by the expression of collagens type I and III, tenascin, and decorin. Gene expression analysis revealed a downregulation of osteogenic and fibrosis markers in the presence of TROPO when compared with the control groups, suggesting proper ECM deposition. Remarkably, differentiated cells exposed to TROPO acquired an elastogenic profile due to the evident elastin synthesis and deposition, contributing to the formation of a more mimetic matrix in comparison with the PDA-coated and uncoated conditions. In summary, our biomimetic substrates combining biophysical and biological cues modulate stem cell behavior potentiating their long-term tenogenic commitment and the production of an elastin-rich ECM.
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Tropoelastin-Coated Tendon Biomimetic Scaffolds Promote Stem Cell Tenogenic Commitment and Deposition of Elastin-Rich Matrix
2019Co-Authors: Helena Almeida, Rui M. A. Domingues, Suzanne M. Mithieux, Ricardo A. Pires, Ana I. Gonçalves, Manuel Gómez-florit, Rui L. Reis, Anthony S. Weiss, Manuela E. GomesAbstract:Tendon tissue engineering strategies that recreate the biophysical and biochemical native microenvironment have a greater potential to achieve regeneration. Here, we developed tendon biomimetic scaffolds using mechanically competent yarns of poly-ε-caprolactone, chitosan, and cellulose nanocrystals to recreate the inherent tendon hierarchy from a nano-to-macro scale. These were then coated with tropoelastin (TROPO) through polydopamine (PDA) linking, to mimic the native extracellular matrix (ECM) composition and elasticity. Both PDA and TROPO coatings decreased Surface Stiffness without masking the underlying substrate. We found that human adipose-derived stem cells (hASCs) seeded onto these TROPO biomimetic scaffolds more rapidly acquired their spindle-shape morphology and high aspect ratio characteristic of tenocytes. Immunocytochemistry shows that the PDA and TROPO-coated Surfaces boosted differentiation of hASCs toward the tenogenic lineage, with sustained expression of the tendon-related markers scleraxis and tenomodulin up to 21 days of culture. Furthermore, these Surfaces enabled the deposition of a tendon-like ECM, supported by the expression of collagens type I and III, tenascin, and decorin. Gene expression analysis revealed a downregulation of osteogenic and fibrosis markers in the presence of TROPO when compared with the control groups, suggesting proper ECM deposition. Remarkably, differentiated cells exposed to TROPO acquired an elastogenic profile due to the evident elastin synthesis and deposition, contributing to the formation of a more mimetic matrix in comparison with the PDA-coated and uncoated conditions. In summary, our biomimetic substrates combining biophysical and biological cues modulate stem cell behavior potentiating their long-term tenogenic commitment and the production of an elastin-rich ECM
