The Experts below are selected from a list of 102 Experts worldwide ranked by ideXlab platform
Shinsuke Kikuchi - One of the best experts on this subject based on the ideXlab platform.
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Surgical Marking Pen dye inhibits saphenous vein cell proliferation and migration in saphenous vein graft tissue
Journal of Vascular Surgery, 2016Co-Authors: Shinsuke Kikuchi, Richard D Kenagy, Thomas N Wight, Michael Sobel, Nobuyoshi Azuma, Alexander W. ClowesAbstract:Objective Markers containing dyes such as crystal violet (CAS 548-62-9) are routinely used on the adventitia of vein bypass grafts to avoid twisting during placement. Because little is known about how these dyes affect vein graft healing and function, we determined the effect of crystal violet on cell migration and proliferation, which are responses to injury after grafting. Methods Fresh human saphenous veins were obtained as residual specimens from leg bypass surgeries. Portions of the vein that had been Surgically marked with crystal violet were analyzed separately from those that had no dye Marking. In the laboratory, they were split into easily dissected inner and outer layers after removal of endothelium. This cleavage plane was within the circular muscle layer of the media. Cell migration from explants was measured daily as either (1) percentage of migration-positive explants, which exclusively measures migration, or (2) number of cells on the plastic surrounding each explant, which measures migration plus proliferation. Cell proliferation and apoptosis (Ki67 and TUNEL staining, respectively) were determined in dye-marked and unmarked areas of cultured vein rings. The dose-dePendent effects of crystal violet were measured for cell migration from explants as well as for proliferation, migration, and death of cultured outer layer cells. Dye was extracted from explants with ethanol and quantified by spectrophotometry. Results There was significantly less cell migration from visibly blue compared with unstained outer layer explants by both methods. There was no significant difference in migration from inner layer explants adjacent to blue-stained or unstained sections of vein because dye did not Penetrate to the inner layer. Ki67 staining of vein in organ culture, which is a measure of proliferation, progressively increased up to 6 days in nonblue outer layer and was abolished in the blue outer layer. Evidence of apoptosis (TUNEL staining) was present throughout the wall and not different in blue-stained and unstained vein wall segments. Blue outer layer explants had 65.9 ± 8.0 ng dye/explant compared with 2.1 ± 1.3 for nonblue outer layer explants. Dye applied in vitro to either outer or inner layer explants dose dePendently inhibited migration (IC 50 ∼10 ng/explant). The IC 50 s of crystal violet for outer layer cell proliferation and migration were 0.1 and 1.2 μg/mL, whereas the EC 50 for death was between 1 and 10 μg/mL. Conclusions Crystal violet inhibits venous cell migration and proliferation, indicating that alternative methods should be considered for Marking vein grafts.
Alexander W. Clowes - One of the best experts on this subject based on the ideXlab platform.
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Surgical Marking Pen dye inhibits saphenous vein cell proliferation and migration in saphenous vein graft tissue
Journal of Vascular Surgery, 2016Co-Authors: Shinsuke Kikuchi, Richard D Kenagy, Thomas N Wight, Michael Sobel, Nobuyoshi Azuma, Alexander W. ClowesAbstract:Objective Markers containing dyes such as crystal violet (CAS 548-62-9) are routinely used on the adventitia of vein bypass grafts to avoid twisting during placement. Because little is known about how these dyes affect vein graft healing and function, we determined the effect of crystal violet on cell migration and proliferation, which are responses to injury after grafting. Methods Fresh human saphenous veins were obtained as residual specimens from leg bypass surgeries. Portions of the vein that had been Surgically marked with crystal violet were analyzed separately from those that had no dye Marking. In the laboratory, they were split into easily dissected inner and outer layers after removal of endothelium. This cleavage plane was within the circular muscle layer of the media. Cell migration from explants was measured daily as either (1) percentage of migration-positive explants, which exclusively measures migration, or (2) number of cells on the plastic surrounding each explant, which measures migration plus proliferation. Cell proliferation and apoptosis (Ki67 and TUNEL staining, respectively) were determined in dye-marked and unmarked areas of cultured vein rings. The dose-dePendent effects of crystal violet were measured for cell migration from explants as well as for proliferation, migration, and death of cultured outer layer cells. Dye was extracted from explants with ethanol and quantified by spectrophotometry. Results There was significantly less cell migration from visibly blue compared with unstained outer layer explants by both methods. There was no significant difference in migration from inner layer explants adjacent to blue-stained or unstained sections of vein because dye did not Penetrate to the inner layer. Ki67 staining of vein in organ culture, which is a measure of proliferation, progressively increased up to 6 days in nonblue outer layer and was abolished in the blue outer layer. Evidence of apoptosis (TUNEL staining) was present throughout the wall and not different in blue-stained and unstained vein wall segments. Blue outer layer explants had 65.9 ± 8.0 ng dye/explant compared with 2.1 ± 1.3 for nonblue outer layer explants. Dye applied in vitro to either outer or inner layer explants dose dePendently inhibited migration (IC 50 ∼10 ng/explant). The IC 50 s of crystal violet for outer layer cell proliferation and migration were 0.1 and 1.2 μg/mL, whereas the EC 50 for death was between 1 and 10 μg/mL. Conclusions Crystal violet inhibits venous cell migration and proliferation, indicating that alternative methods should be considered for Marking vein grafts.
Goksel M - One of the best experts on this subject based on the ideXlab platform.
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Tracing the dural tail with image-guided surgery.
Minimally invasive neurosurgery : MIN, 2006Co-Authors: Semih Keskil, Bademci G, Goksel MAbstract:Objective: The extent of dural resection is important for preventing recurrence in meningioma management. An image-guidance assisted technique is described to perform adequate dural resection. Methods: A universal instrument adapter system for image-guidance was used to track the dural extension of the meningioma accurately. Results: The universal instrument adapter offers the surgeon the possibility to image-guide nearly any rigid instrument via the computed calibration method. In this way a Surgical Marking Pen was used to chase and adequately mark the "dural tail". Discussion: Image-guidance systems can be used to avoid incomplete resection of the affected dura that may be responsible for tumour recurrence.
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Tracing the dural tail with image-guided surgery
'Georg Thieme Verlag KG', 2006Co-Authors: Keskil S., Bademci G, Goksel MAbstract:WOS: 000245192900008PubMed: 17323263Objective: The extent of dural resection is important for preventing recurrence in meningioma management. An image-guidance assisted technique is described to perform adequate dural resection. Methods: A universal instrument adapter system for image-guidance was used to track the dural extension of the meningioma accurately. Results: The universal instrument adapter offers the surgeon the possibility to image-guide nearly any rigid instrument via the computed calibration method. in this way a Surgical Marking Pen was used to chase and adequately mark the "dural tail". Discussion: Image-guidance systems can be used to avoid incomplete resection of the affected dura that may be responsible for tumour recurrence
Semih Keskil - One of the best experts on this subject based on the ideXlab platform.
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Tracing the dural tail with image-guided surgery.
Minimally invasive neurosurgery : MIN, 2006Co-Authors: Semih Keskil, Bademci G, Goksel MAbstract:Objective: The extent of dural resection is important for preventing recurrence in meningioma management. An image-guidance assisted technique is described to perform adequate dural resection. Methods: A universal instrument adapter system for image-guidance was used to track the dural extension of the meningioma accurately. Results: The universal instrument adapter offers the surgeon the possibility to image-guide nearly any rigid instrument via the computed calibration method. In this way a Surgical Marking Pen was used to chase and adequately mark the "dural tail". Discussion: Image-guidance systems can be used to avoid incomplete resection of the affected dura that may be responsible for tumour recurrence.
Nobuyoshi Azuma - One of the best experts on this subject based on the ideXlab platform.
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Surgical Marking Pen dye inhibits saphenous vein cell proliferation and migration in saphenous vein graft tissue
Journal of Vascular Surgery, 2016Co-Authors: Shinsuke Kikuchi, Richard D Kenagy, Thomas N Wight, Michael Sobel, Nobuyoshi Azuma, Alexander W. ClowesAbstract:Objective Markers containing dyes such as crystal violet (CAS 548-62-9) are routinely used on the adventitia of vein bypass grafts to avoid twisting during placement. Because little is known about how these dyes affect vein graft healing and function, we determined the effect of crystal violet on cell migration and proliferation, which are responses to injury after grafting. Methods Fresh human saphenous veins were obtained as residual specimens from leg bypass surgeries. Portions of the vein that had been Surgically marked with crystal violet were analyzed separately from those that had no dye Marking. In the laboratory, they were split into easily dissected inner and outer layers after removal of endothelium. This cleavage plane was within the circular muscle layer of the media. Cell migration from explants was measured daily as either (1) percentage of migration-positive explants, which exclusively measures migration, or (2) number of cells on the plastic surrounding each explant, which measures migration plus proliferation. Cell proliferation and apoptosis (Ki67 and TUNEL staining, respectively) were determined in dye-marked and unmarked areas of cultured vein rings. The dose-dePendent effects of crystal violet were measured for cell migration from explants as well as for proliferation, migration, and death of cultured outer layer cells. Dye was extracted from explants with ethanol and quantified by spectrophotometry. Results There was significantly less cell migration from visibly blue compared with unstained outer layer explants by both methods. There was no significant difference in migration from inner layer explants adjacent to blue-stained or unstained sections of vein because dye did not Penetrate to the inner layer. Ki67 staining of vein in organ culture, which is a measure of proliferation, progressively increased up to 6 days in nonblue outer layer and was abolished in the blue outer layer. Evidence of apoptosis (TUNEL staining) was present throughout the wall and not different in blue-stained and unstained vein wall segments. Blue outer layer explants had 65.9 ± 8.0 ng dye/explant compared with 2.1 ± 1.3 for nonblue outer layer explants. Dye applied in vitro to either outer or inner layer explants dose dePendently inhibited migration (IC 50 ∼10 ng/explant). The IC 50 s of crystal violet for outer layer cell proliferation and migration were 0.1 and 1.2 μg/mL, whereas the EC 50 for death was between 1 and 10 μg/mL. Conclusions Crystal violet inhibits venous cell migration and proliferation, indicating that alternative methods should be considered for Marking vein grafts.