The Experts below are selected from a list of 81627 Experts worldwide ranked by ideXlab platform
Dieter Manstein - One of the best experts on this subject based on the ideXlab platform.
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thermal injury causes dna damage and lethality in unheated Surrounding cells active thermal bystander effect
Journal of Investigative Dermatology, 2010Co-Authors: M L Purschke, Rox R Anderson, Hansjoachim Laubach, Dieter MansteinAbstract:Direct heat exposure to cells causes protein degradation and DNA damage, which can lead to genetic alteration and cell death, but little is known about heat-induced effects on the Surrounding Tissue. After burns or laser surgery, loss of viability in the Surrounding Tissue has been explained by a temperature gradient due to heat diffusion. This study shows that, in the absence of any direct heating, heat diffusion, or cell-to-cell contact, "bystander" cells that share the medium with heat-exposed cells exhibit DNA damage, apoptosis, and loss of viability. We coin this phenomenon "active thermal bystander effect" (ATBE). Significant ATBE was induced by fibroblasts exposed for 10 minutes to a temperature range of 44-50 degrees C (all P<0.011). The ATBE was not induced by cells heated to lethality above 54 degrees C and immediate medium exchange did not suppress the effect. Therefore, the thermal bystander effect appears to be an active process in which viable, heat-injured cells induce a signal cascade and/or mediator that damages or kills Surrounding bystander cells. The ATBE may have clinical relevance for acute burn trauma, hyperthermic treatments, and distant Tissue damage after localized heat stress.
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thermal injury causes dna damage and lethality in unheated Surrounding cells active thermal bystander effect
Journal of Investigative Dermatology, 2010Co-Authors: M L Purschke, Rox R Anderson, Hansjoachim Laubach, Dieter MansteinAbstract:Direct heat exposure to cells causes protein degradation and DNA damage, which can lead to genetic alteration and cell death, but little is known about heat-induced effects on the Surrounding Tissue. After burns or laser surgery, loss of viability in the Surrounding Tissue has been explained by a temperature gradient due to heat diffusion. This study shows that, in the absence of any direct heating, heat diffusion, or cell-to-cell contact, "bystander" cells that share the medium with heat-exposed cells exhibit DNA damage, apoptosis, and loss of viability. We coin this phenomenon "active thermal bystander effect" (ATBE). Significant ATBE was induced by fibroblasts exposed for 10minutes to a temperature range of 44–50°C (all P
Eric E Smouha - One of the best experts on this subject based on the ideXlab platform.
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high resolution microendoscope images of middle ear cholesteatoma and Surrounding Tissue evaluation of interobserver concordance
Otolaryngology-Head and Neck Surgery, 2013Co-Authors: James Bradley, Lauren L Levy, Rebecca Richardskortum, Andrew G Sikora, Nancy Jiang, Eric E SmouhaAbstract:ObjectiveInvestigate how accurately otolaryngologists could differentiate between images obtained with high-resolution microendoscopy (HRME) of ex vivo cholesteatoma specimens and Surrounding middle ear epithelium.Study DesignHRME images of surgically resected cholesteatoma and middle ear epithelium were obtained and otolaryngologists classified these images.SettingTertiary medical center.Subjects and MethodsResected cholesteatoma and middle ear epithelium were stained with a contrast agent, proflavine, and HRME images were captured. Specimens were sent for standard histopathology and compared with HRME images. Quality-controlled images were used to assemble a training set. After viewing training images, otolaryngologists without prior cholesteatoma HRME experience reviewed and classified test images.ResultsTen cholesteatoma and 9 middle ear specimens were collected, of which 17 representative cholesteatoma and 19 middle ear epithelium images were extracted for a testing set. Qualitative analysis for conc...
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comparison of high resolution microendoscope images and histopathological sections in ex vivo middle ear cholesteatomas and Surrounding Tissue
Proceedings of SPIE, 2013Co-Authors: James Bradley, Lauren L Levy, Rebecca Richardskortum, Andrew G Sikora, Eric E SmouhaAbstract:Objective: To investigate the concordance between optical images obtained with high-resolution microendoscopy (HRME) and conventional histopathology for ex vivo cholesteatoma specimens and Surrounding middle ear epithelium. Methods: After resection of cholesteatoma and Surrounding middle ear epithelium from surgical patients, Tissues were stained with a contrast agent, proflavine, and the HRME fiberoptic scope was placed directly on each Tissue specimen. 4- 10 short movie clips were recorded for both the cholesteatoma and Surrounding middle ear epithelium specimens. The imaged areas were sent for standard histopathology, and the stained specimens were correlated with the HRME images. IRB approval was obtained, and each patient was consented for the study. Results: Ten cholesteatoma specimens and 9 middle ear specimens were collected from 10 patients. In each case, cholesteatoma was easily discriminated from normal middle ear epithelium by its hyperfluorescence and loss of cellular detail. Qualitative analysis for concordance between HRME images and histological images from the same surgical specimen yielded a strong correlation between imaging modalities. Conclusions: Keratinizing cholesteatoma and Surrounding middle ear epithelium have distinct imaging characteristics. Loss of cellular detail and hyperfluorescence with proflavine are the hallmark characteristics of cholesteatoma which allow for differentiation from normal middle ear epithelium. Real-time optical imaging can potentially improve the results of otologic surgery by allowing for extirpation of cholesteatomas while eliminating residual disease. We anticipate performing an in vivo study to test this hypothesis.
M L Purschke - One of the best experts on this subject based on the ideXlab platform.
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thermal injury causes dna damage and lethality in unheated Surrounding cells active thermal bystander effect
Journal of Investigative Dermatology, 2010Co-Authors: M L Purschke, Rox R Anderson, Hansjoachim Laubach, Dieter MansteinAbstract:Direct heat exposure to cells causes protein degradation and DNA damage, which can lead to genetic alteration and cell death, but little is known about heat-induced effects on the Surrounding Tissue. After burns or laser surgery, loss of viability in the Surrounding Tissue has been explained by a temperature gradient due to heat diffusion. This study shows that, in the absence of any direct heating, heat diffusion, or cell-to-cell contact, "bystander" cells that share the medium with heat-exposed cells exhibit DNA damage, apoptosis, and loss of viability. We coin this phenomenon "active thermal bystander effect" (ATBE). Significant ATBE was induced by fibroblasts exposed for 10 minutes to a temperature range of 44-50 degrees C (all P<0.011). The ATBE was not induced by cells heated to lethality above 54 degrees C and immediate medium exchange did not suppress the effect. Therefore, the thermal bystander effect appears to be an active process in which viable, heat-injured cells induce a signal cascade and/or mediator that damages or kills Surrounding bystander cells. The ATBE may have clinical relevance for acute burn trauma, hyperthermic treatments, and distant Tissue damage after localized heat stress.
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thermal injury causes dna damage and lethality in unheated Surrounding cells active thermal bystander effect
Journal of Investigative Dermatology, 2010Co-Authors: M L Purschke, Rox R Anderson, Hansjoachim Laubach, Dieter MansteinAbstract:Direct heat exposure to cells causes protein degradation and DNA damage, which can lead to genetic alteration and cell death, but little is known about heat-induced effects on the Surrounding Tissue. After burns or laser surgery, loss of viability in the Surrounding Tissue has been explained by a temperature gradient due to heat diffusion. This study shows that, in the absence of any direct heating, heat diffusion, or cell-to-cell contact, "bystander" cells that share the medium with heat-exposed cells exhibit DNA damage, apoptosis, and loss of viability. We coin this phenomenon "active thermal bystander effect" (ATBE). Significant ATBE was induced by fibroblasts exposed for 10minutes to a temperature range of 44–50°C (all P
James Bradley - One of the best experts on this subject based on the ideXlab platform.
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high resolution microendoscope images of middle ear cholesteatoma and Surrounding Tissue evaluation of interobserver concordance
Otolaryngology-Head and Neck Surgery, 2013Co-Authors: James Bradley, Lauren L Levy, Rebecca Richardskortum, Andrew G Sikora, Nancy Jiang, Eric E SmouhaAbstract:ObjectiveInvestigate how accurately otolaryngologists could differentiate between images obtained with high-resolution microendoscopy (HRME) of ex vivo cholesteatoma specimens and Surrounding middle ear epithelium.Study DesignHRME images of surgically resected cholesteatoma and middle ear epithelium were obtained and otolaryngologists classified these images.SettingTertiary medical center.Subjects and MethodsResected cholesteatoma and middle ear epithelium were stained with a contrast agent, proflavine, and HRME images were captured. Specimens were sent for standard histopathology and compared with HRME images. Quality-controlled images were used to assemble a training set. After viewing training images, otolaryngologists without prior cholesteatoma HRME experience reviewed and classified test images.ResultsTen cholesteatoma and 9 middle ear specimens were collected, of which 17 representative cholesteatoma and 19 middle ear epithelium images were extracted for a testing set. Qualitative analysis for conc...
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comparison of high resolution microendoscope images and histopathological sections in ex vivo middle ear cholesteatomas and Surrounding Tissue
Proceedings of SPIE, 2013Co-Authors: James Bradley, Lauren L Levy, Rebecca Richardskortum, Andrew G Sikora, Eric E SmouhaAbstract:Objective: To investigate the concordance between optical images obtained with high-resolution microendoscopy (HRME) and conventional histopathology for ex vivo cholesteatoma specimens and Surrounding middle ear epithelium. Methods: After resection of cholesteatoma and Surrounding middle ear epithelium from surgical patients, Tissues were stained with a contrast agent, proflavine, and the HRME fiberoptic scope was placed directly on each Tissue specimen. 4- 10 short movie clips were recorded for both the cholesteatoma and Surrounding middle ear epithelium specimens. The imaged areas were sent for standard histopathology, and the stained specimens were correlated with the HRME images. IRB approval was obtained, and each patient was consented for the study. Results: Ten cholesteatoma specimens and 9 middle ear specimens were collected from 10 patients. In each case, cholesteatoma was easily discriminated from normal middle ear epithelium by its hyperfluorescence and loss of cellular detail. Qualitative analysis for concordance between HRME images and histological images from the same surgical specimen yielded a strong correlation between imaging modalities. Conclusions: Keratinizing cholesteatoma and Surrounding middle ear epithelium have distinct imaging characteristics. Loss of cellular detail and hyperfluorescence with proflavine are the hallmark characteristics of cholesteatoma which allow for differentiation from normal middle ear epithelium. Real-time optical imaging can potentially improve the results of otologic surgery by allowing for extirpation of cholesteatomas while eliminating residual disease. We anticipate performing an in vivo study to test this hypothesis.
Dov Jaron - One of the best experts on this subject based on the ideXlab platform.
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a model of no o2 transport in capillary perfused Tissue containing an arteriole and venule pair
Annals of Biomedical Engineering, 2007Co-Authors: Xuewen Chen, Kenneth A. Barbee, Donald G. Buerk, Dov JaronAbstract:The goal of this study was to investigate the complex co-transport of nitric oxide (NO) and oxygen (O2) in a paired arteriole–venule, surrounded by capillary-perfused Tissue using a computer model. Blood flow was assumed to be steady in the arteriolar and venular lumens and to obey Darcy’s law in the Tissue. NO consumption rate was assumed to be constant in the core of the arteriolar and venular lumen and to decrease linearly to the endothelium. Average NO consumption rate by capillary blood in a unit Tissue volume was assumed proportional to the blood flux across the volume. Our results predict that: (1) the capillary bed, which connects the arteriole and venule, facilitates the release of O2 from the vessel pair to the Surrounding Tissue; (2) decreasing the distance between arteriole and venule can result in a higher NO concentration in the venular wall than in the arteriolar wall; (3) in the absence of capillaries in the Surrounding Tissue, diffusion of NO from venule to arteriole contributes little to NO concentration in the arteriolar wall; and (4) when capillaries are added to the simulation, a significant increase of NO in the arteriolar wall is observed.
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a model of no o 2 transport in capillary perfused Tissue containing an arteriole and venule pair
International Conference of the IEEE Engineering in Medicine and Biology Society, 2005Co-Authors: Xuewen Chen, Kenneth A. Barbee, Donald G. Buerk, Dov JaronAbstract:The goal of this study was to investigate the complex co-transport of nitric oxide (NO) and oxygen (O2) in a paired arteriole-venule, surrounded by capillary-perfused Tissue using a computer model. Blood flow was assumed to be steady in the arteriole and venular lumens and to obey Darcy's law in the capillary-perfused Tissue. NO consumption rate in the arteriolar and venular lumen was assumed to be constant in the core of the arteriolar and venular lumen and to decrease linearly to the endothelium. Average NO consumption rate by capillary blood in a unit Tissue volume was assumed proportional to the blood flux across the volume. Our preliminary results predict that: 1) The capillary bed, which connects the arteriole and venule, facilitates the release of O2 from the vessel pair to the Surrounding Tissue; 2) Decreasing the distance between arteriole and venule can result in a higher NO concentration in the venular wall than in the arteriolar wall; 3) In the absence of capillaries in the Surrounding Tissue, diffusion of NO from venule to arteriole contributes little to NO contents in the arteriole; and 4) when capillaries are added to the simulation, a significant increase in arteriole NO content is observed
