The Experts below are selected from a list of 198 Experts worldwide ranked by ideXlab platform
Magnus Johansson - One of the best experts on this subject based on the ideXlab platform.
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Revealing new tick-borne encephalitis virus foci by screening antibodies in sheep milk
Parasites & Vectors, 2020Co-Authors: Amélie Wallenhammar, Richard Lindqvist, Naveed Asghar, Sezin Gunaltay, Hans Fredlund, Åke Davidsson, Sören Andersson, Anna K. Överby, Magnus JohanssonAbstract:Background Tick distribution in Sweden has increased in recent years, with the prevalence of ticks predicted to spread towards the northern parts of the country, thus increasing the risk of tick-borne zoonoses in new regions. Tick-borne encephalitis (TBE) is the most significant viral tick-borne zoonotic disease in Europe. The disease is caused by TBE virus (TBEV) infection which often leads to severe encephalitis and myelitis in humans. TBEV is usually transmitted to humans via tick bites; however, the virus can also be excreted in the milk of goats, sheep and cattle and infection may then occur via consumption of unpasteurised dairy products. Virus prevalence in questing ticks is an unreliable indicator of TBE infection risk as viral RNA is rarely detected even in large sample sizes collected at TBE-endemic areas. Hence, there is a need for robust Surveillance Techniques to identify emerging TBEV risk areas at early stages. Methods Milk and colostrum samples were collected from sheep and goats in Örebro County, Sweden. The milk samples were analysed for the presence of TBEV antibodies by ELISA and validated by western blot in which milk samples were used to detect over-expressed TBEV E-protein in crude cell extracts. Neutralising titers were determined by focus reduction neutralisation test (FRNT). The stability of TBEV in milk and colostrum was studied at different temperatures. Results In this study we have developed a novel strategy to identify new TBEV foci. By monitoring TBEV antibodies in milk, we have identified three previously unknown foci in Örebro County which also overlap with areas of TBE infection reported during 2009–2018. In addition, our data indicates that keeping unpasteurised milk at 4 °C will preserve the infectivity of TBEV for several days. Conclusions Altogether, we report a non-invasive Surveillance Technique for revealing risk areas for TBE in Sweden, by detecting TBEV antibodies in sheep milk. This approach is robust and reliable and can accordingly be used to map TBEV “hotspots”. TBEV infectivity in refrigerated milk was preserved, emphasising the importance of pasteurisation (i.e. 72 °C for 15 s) prior to consumption.
Amélie Wallenhammar - One of the best experts on this subject based on the ideXlab platform.
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Revealing new tick-borne encephalitis virus foci by screening antibodies in sheep milk
Parasites & Vectors, 2020Co-Authors: Amélie Wallenhammar, Richard Lindqvist, Naveed Asghar, Sezin Gunaltay, Hans Fredlund, Åke Davidsson, Sören Andersson, Anna K. Överby, Magnus JohanssonAbstract:Background Tick distribution in Sweden has increased in recent years, with the prevalence of ticks predicted to spread towards the northern parts of the country, thus increasing the risk of tick-borne zoonoses in new regions. Tick-borne encephalitis (TBE) is the most significant viral tick-borne zoonotic disease in Europe. The disease is caused by TBE virus (TBEV) infection which often leads to severe encephalitis and myelitis in humans. TBEV is usually transmitted to humans via tick bites; however, the virus can also be excreted in the milk of goats, sheep and cattle and infection may then occur via consumption of unpasteurised dairy products. Virus prevalence in questing ticks is an unreliable indicator of TBE infection risk as viral RNA is rarely detected even in large sample sizes collected at TBE-endemic areas. Hence, there is a need for robust Surveillance Techniques to identify emerging TBEV risk areas at early stages. Methods Milk and colostrum samples were collected from sheep and goats in Örebro County, Sweden. The milk samples were analysed for the presence of TBEV antibodies by ELISA and validated by western blot in which milk samples were used to detect over-expressed TBEV E-protein in crude cell extracts. Neutralising titers were determined by focus reduction neutralisation test (FRNT). The stability of TBEV in milk and colostrum was studied at different temperatures. Results In this study we have developed a novel strategy to identify new TBEV foci. By monitoring TBEV antibodies in milk, we have identified three previously unknown foci in Örebro County which also overlap with areas of TBE infection reported during 2009–2018. In addition, our data indicates that keeping unpasteurised milk at 4 °C will preserve the infectivity of TBEV for several days. Conclusions Altogether, we report a non-invasive Surveillance Technique for revealing risk areas for TBE in Sweden, by detecting TBEV antibodies in sheep milk. This approach is robust and reliable and can accordingly be used to map TBEV “hotspots”. TBEV infectivity in refrigerated milk was preserved, emphasising the importance of pasteurisation (i.e. 72 °C for 15 s) prior to consumption.
Xiaochun Zhang - One of the best experts on this subject based on the ideXlab platform.
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The research of stress monitor and broken testing for steel wire rope
2009 9th International Conference on Electronic Measurement & Instruments, 2009Co-Authors: Xiaochun ZhangAbstract:To estimate the security of steel wire rope, investigated the broken wire condition, the capability of the rope to bear weight and the state of stress balance of each wire in the steel wire rope. The wavelet translation method has been applied to analyze the signals of the leakage magnetic field from the steel wire rope. The result of the time-frequency analysis of the signals can be used to make certain the position and the amount of the broken wire. According to the static Surveillance method, we designed the dynamic Surveillance method to detect the stress balance of the steel wire rope. This technology makes it possible to check the stress condition of each wire on line. We conclude that the wavelet translation analysis and the dynamic Surveillance Technique are effective methods to detect on line and real-time the broken wire and the stress balance of many-wire.
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The technology of testing the safety of steel wire ropes
ICMIT 2005: Information Systems and Signal Processing, 2005Co-Authors: Xiaochun ZhangAbstract:To estimate the security of steel wire rope, the broken wire condition, the capability of the rope to bear weight and the state of stress balance of each wire in the steel wire rope were investigated. The wavelet translation method was applied to analyze the signals of magnetic field leakage from the steel wire rope. The result of the time-frequency analysis of the signals can be used to make certain of he position and the amount of the broken wire. Using the static Surveillance method as a basis, a dynamic Surveillance method was designed to detect the stress balance of the steel wire rope. This technology makes it possible to check the stress condition of each wire on line. It can be concluded that a wavelet translation analysis and the dynamic Surveillance Technique are effective methods to detect on line and real-time the broken wire and the stress balance of multistrand wire ropes.
Naveed Asghar - One of the best experts on this subject based on the ideXlab platform.
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Revealing new tick-borne encephalitis virus foci by screening antibodies in sheep milk
Parasites & Vectors, 2020Co-Authors: Amélie Wallenhammar, Richard Lindqvist, Naveed Asghar, Sezin Gunaltay, Hans Fredlund, Åke Davidsson, Sören Andersson, Anna K. Överby, Magnus JohanssonAbstract:Background Tick distribution in Sweden has increased in recent years, with the prevalence of ticks predicted to spread towards the northern parts of the country, thus increasing the risk of tick-borne zoonoses in new regions. Tick-borne encephalitis (TBE) is the most significant viral tick-borne zoonotic disease in Europe. The disease is caused by TBE virus (TBEV) infection which often leads to severe encephalitis and myelitis in humans. TBEV is usually transmitted to humans via tick bites; however, the virus can also be excreted in the milk of goats, sheep and cattle and infection may then occur via consumption of unpasteurised dairy products. Virus prevalence in questing ticks is an unreliable indicator of TBE infection risk as viral RNA is rarely detected even in large sample sizes collected at TBE-endemic areas. Hence, there is a need for robust Surveillance Techniques to identify emerging TBEV risk areas at early stages. Methods Milk and colostrum samples were collected from sheep and goats in Örebro County, Sweden. The milk samples were analysed for the presence of TBEV antibodies by ELISA and validated by western blot in which milk samples were used to detect over-expressed TBEV E-protein in crude cell extracts. Neutralising titers were determined by focus reduction neutralisation test (FRNT). The stability of TBEV in milk and colostrum was studied at different temperatures. Results In this study we have developed a novel strategy to identify new TBEV foci. By monitoring TBEV antibodies in milk, we have identified three previously unknown foci in Örebro County which also overlap with areas of TBE infection reported during 2009–2018. In addition, our data indicates that keeping unpasteurised milk at 4 °C will preserve the infectivity of TBEV for several days. Conclusions Altogether, we report a non-invasive Surveillance Technique for revealing risk areas for TBE in Sweden, by detecting TBEV antibodies in sheep milk. This approach is robust and reliable and can accordingly be used to map TBEV “hotspots”. TBEV infectivity in refrigerated milk was preserved, emphasising the importance of pasteurisation (i.e. 72 °C for 15 s) prior to consumption.
Åke Davidsson - One of the best experts on this subject based on the ideXlab platform.
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Revealing new tick-borne encephalitis virus foci by screening antibodies in sheep milk
Parasites & Vectors, 2020Co-Authors: Amélie Wallenhammar, Richard Lindqvist, Naveed Asghar, Sezin Gunaltay, Hans Fredlund, Åke Davidsson, Sören Andersson, Anna K. Överby, Magnus JohanssonAbstract:Background Tick distribution in Sweden has increased in recent years, with the prevalence of ticks predicted to spread towards the northern parts of the country, thus increasing the risk of tick-borne zoonoses in new regions. Tick-borne encephalitis (TBE) is the most significant viral tick-borne zoonotic disease in Europe. The disease is caused by TBE virus (TBEV) infection which often leads to severe encephalitis and myelitis in humans. TBEV is usually transmitted to humans via tick bites; however, the virus can also be excreted in the milk of goats, sheep and cattle and infection may then occur via consumption of unpasteurised dairy products. Virus prevalence in questing ticks is an unreliable indicator of TBE infection risk as viral RNA is rarely detected even in large sample sizes collected at TBE-endemic areas. Hence, there is a need for robust Surveillance Techniques to identify emerging TBEV risk areas at early stages. Methods Milk and colostrum samples were collected from sheep and goats in Örebro County, Sweden. The milk samples were analysed for the presence of TBEV antibodies by ELISA and validated by western blot in which milk samples were used to detect over-expressed TBEV E-protein in crude cell extracts. Neutralising titers were determined by focus reduction neutralisation test (FRNT). The stability of TBEV in milk and colostrum was studied at different temperatures. Results In this study we have developed a novel strategy to identify new TBEV foci. By monitoring TBEV antibodies in milk, we have identified three previously unknown foci in Örebro County which also overlap with areas of TBE infection reported during 2009–2018. In addition, our data indicates that keeping unpasteurised milk at 4 °C will preserve the infectivity of TBEV for several days. Conclusions Altogether, we report a non-invasive Surveillance Technique for revealing risk areas for TBE in Sweden, by detecting TBEV antibodies in sheep milk. This approach is robust and reliable and can accordingly be used to map TBEV “hotspots”. TBEV infectivity in refrigerated milk was preserved, emphasising the importance of pasteurisation (i.e. 72 °C for 15 s) prior to consumption.