The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Gary F. Gerard - One of the best experts on this subject based on the ideXlab platform.
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mutation detection using Surveyor nuclease
BioTechniques, 2004Co-Authors: Harini Shandilya, Jeffrey Durocher, James M Dalessio, Kevin C Oconnor, Gary F. GerardAbstract:We have developed a simple and flexible mutation detection technology for the discovery and mapping of both known and unknown mutations. This technology is based on a new mismatch-specific DNA endo...
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Mutation detection using Surveyor™ nuclease
BioTechniques, 2004Co-Authors: Peter Qiu, Harini Shandilya, James M. D'alessio, Jeffrey Durocher, Kevin O'connor, Gary F. GerardAbstract:We have developed a simple and flexible mutation detection technology for the discovery and mapping of both known and unknown mutations. This technology is based on a new mismatch-specific DNA endonuclease from celery, Surveyor nuclease, which is a member of the CEL nuclease family of plant DNA endonucleases. Surveyor nuclease cleaves with high specificity at the 3' side of any mismatch site in both DNA strands, including all base substitutions and insertion/deletions up to at least 12 nucleotides. Surveyor nuclease technology involves four steps: (i) PCR to amplify target DNA from both mutant and wild-type reference DNA; (ii) hybridization to form heteroduplexes between mutant and wild-type reference DNA; (iii) treatment of annealed DNA with Surveyor nuclease to cleave heteroduplexes; and (iv) analysis of digested DNA products using the detection/separation platform of choice. The technology is highly sensitive, detecting rare mutants present at as low as 1 in 32 copies. Unlabeled Surveyor nuclease digestion products can be analyzed using conventional gel electrophoresis or high-performance liquid chromatography (HPLC), while end labeled digestion products are suitable for analysis by automated gel or capillary electrophoresis. The entire protocol can be performed in less than a day and is suitable for automated and high-throughput procedures.
Sunao Hasegawa - One of the best experts on this subject based on the ideXlab platform.
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the far infrared Surveyor fis for akari
Publications of the Astronomical Society of Japan, 2007Co-Authors: Mitsunobu Kawada, Hajime Baba, Peter Barthel, D L Clements, Martin Cohen, Yasuo Doi, E Figueredo, Mikio Fujiwara, Tomotsugu Goto, Sunao HasegawaAbstract:The Far-Infrared Surveyor (FIS) is one of two focal-plane instruments on the AKARI satellite. FIS has four photometric bands at 65, 90, 140, and 160 mu m, and uses two kinds of array detectors. The FIS arrays and optics are designed to sweep the sky with high spatial resolution and redundancy. The actual scan width is more than eight arcminutes, and the pixel pitch matches the diffraction limit of the telescope. Derived point-spread functions (PSFs) from observations of asteroids are similar to those given by the optical model. Significant excesses, however, are clearly seen around tails of the PSFs, whose contributions are about 30% of the total power. All FIS functions are operating well in orbit, and the performance meets the laboratory characterizations, except for the two longer wavelength bands, which are not performing as well as characterized. Furthermore, the FIS has a spectroscopic capability using a Fourier transform spectrometer (FTS). Because the FTS takes advantage of the optics and detectors of the photometer, it can simultaneously make a spectral map. This paper summarizes the in-flight technical and operational performance of the FIS.
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the far infrared Surveyor fis for akari
arXiv: Astrophysics, 2007Co-Authors: Mitsunobu Kawada, Hajime Baba, Peter Barthel, D L Clements, Martin Cohen, Yasuo Doi, E Figueredo, Mikio Fujiwara, Tomotsugu Goto, Sunao HasegawaAbstract:The Far-Infrared Surveyor (FIS) is one of two focal plane instruments on the AKARI satellite. FIS has four photometric bands at 65, 90, 140, and 160 um, and uses two kinds of array detectors. The FIS arrays and optics are designed to sweep the sky with high spatial resolution and redundancy. The actual scan width is more than eight arcmin, and the pixel pitch is matches the diffraction limit of the telescope. Derived point spread functions (PSFs) from observations of asteroids are similar to the optical model. Significant excesses, however, are clearly seen around tails of the PSFs, whose contributions are about 30% of the total power. All FIS functions are operating well in orbit, and its performance meets the laboratory characterizations, except for the two longer wavelength bands, which are not performing as well as characterized. Furthermore, the FIS has a spectroscopic capability using a Fourier transform spectrometer (FTS). Because the FTS takes advantage of the optics and detectors of the photometer, it can simultaneously make a spectral map. This paper summarizes the in-flight technical and operational performance of the FIS.
Thomas E. Thorpe - One of the best experts on this subject based on the ideXlab platform.
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Overview of the Mars Global Surveyor mission
Journal of Geophysical Research: Planets, 2001Co-Authors: Arden L. Albee, Frank D. Palluconi, Raymond E. Arvidson, Thomas E. ThorpeAbstract:The Mars Global Surveyor spacecraft was placed into Mars orbit on September 11, 1997, and by March 9, 1999, had slowly circularized through aerobraking to a Sun-synchronous, near-polar orbit with an average altitude of 378 km. The science payload includes the Mars Orbiter Camera, Mars Orbiter Laser Altimeter, Thermal Emission Spectrometer, Ultrastable Oscillator (for Radio Science experiments), and Magnetometer/Electron Reflectometer package. In addition, the spacecraft accelerometers and horizon sensors were used to study atmospheric dynamics during aerobraking. Observations are processed to standard products by the instrument teams and released as documented archive volumes on 6-month centers by the Planetary Data System. Significant results have been obtained from observations of the interior, surface, and atmosphere. For example, Mars does not now have an active magnetic field, although strong remanent magnetization features exist in the ancient crust. These results imply that an internal dynamo ceased operation early in geologic time. Altimetry and gravity data indicate that the crust is thickest under the south pole, thinning northward from the cratered terrain to the northern plains. Analysis of altimetry data demonstrates that Mars is “egg-shaped” with gravitational equipotential contours that show that channel systems in the southern highlands drained to the north, largely to the Chryse trough. A closed contour in the northern plains is consistent with the existence of a great northern ocean. Emission spectra of low-albedo regions show that basaltic rocks dominate spectral signatures on the southern highlands, whereas basaltic andesites dominate the northern lowlands. The bright regions show nondiagnostic spectra, similar to that of dust in the atmosphere. Signatures of aqueous minerals (e.g., clays, carbonates, and sulfates) are noticeably absent from the emission spectra. High spatial resolution images show that the surface has been extensively modified by wind and that layering is nearly ubiquitous, implying that a complex history of events is recorded in surface and near-surface materials. Altimetry data imply that both permanent caps are composed of water ice and dust, with seasonal covers of carbon dioxide frost. Finally, the altimetry data, coupled with thousands of atmospheric profiles, are providing new boundary conditions and dynamic controls for the generation and testing of more realistic dynamic models of the global circulation of the atmosphere.
Harini Shandilya - One of the best experts on this subject based on the ideXlab platform.
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mutation detection using Surveyor nuclease
BioTechniques, 2004Co-Authors: Harini Shandilya, Jeffrey Durocher, James M Dalessio, Kevin C Oconnor, Gary F. GerardAbstract:We have developed a simple and flexible mutation detection technology for the discovery and mapping of both known and unknown mutations. This technology is based on a new mismatch-specific DNA endo...
-
Mutation detection using Surveyor™ nuclease
BioTechniques, 2004Co-Authors: Peter Qiu, Harini Shandilya, James M. D'alessio, Jeffrey Durocher, Kevin O'connor, Gary F. GerardAbstract:We have developed a simple and flexible mutation detection technology for the discovery and mapping of both known and unknown mutations. This technology is based on a new mismatch-specific DNA endonuclease from celery, Surveyor nuclease, which is a member of the CEL nuclease family of plant DNA endonucleases. Surveyor nuclease cleaves with high specificity at the 3' side of any mismatch site in both DNA strands, including all base substitutions and insertion/deletions up to at least 12 nucleotides. Surveyor nuclease technology involves four steps: (i) PCR to amplify target DNA from both mutant and wild-type reference DNA; (ii) hybridization to form heteroduplexes between mutant and wild-type reference DNA; (iii) treatment of annealed DNA with Surveyor nuclease to cleave heteroduplexes; and (iv) analysis of digested DNA products using the detection/separation platform of choice. The technology is highly sensitive, detecting rare mutants present at as low as 1 in 32 copies. Unlabeled Surveyor nuclease digestion products can be analyzed using conventional gel electrophoresis or high-performance liquid chromatography (HPLC), while end labeled digestion products are suitable for analysis by automated gel or capillary electrophoresis. The entire protocol can be performed in less than a day and is suitable for automated and high-throughput procedures.
Arden L. Albee - One of the best experts on this subject based on the ideXlab platform.
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Overview of the Mars Global Surveyor mission
Journal of Geophysical Research: Planets, 2001Co-Authors: Arden L. Albee, Frank D. Palluconi, Raymond E. Arvidson, Thomas E. ThorpeAbstract:The Mars Global Surveyor spacecraft was placed into Mars orbit on September 11, 1997, and by March 9, 1999, had slowly circularized through aerobraking to a Sun-synchronous, near-polar orbit with an average altitude of 378 km. The science payload includes the Mars Orbiter Camera, Mars Orbiter Laser Altimeter, Thermal Emission Spectrometer, Ultrastable Oscillator (for Radio Science experiments), and Magnetometer/Electron Reflectometer package. In addition, the spacecraft accelerometers and horizon sensors were used to study atmospheric dynamics during aerobraking. Observations are processed to standard products by the instrument teams and released as documented archive volumes on 6-month centers by the Planetary Data System. Significant results have been obtained from observations of the interior, surface, and atmosphere. For example, Mars does not now have an active magnetic field, although strong remanent magnetization features exist in the ancient crust. These results imply that an internal dynamo ceased operation early in geologic time. Altimetry and gravity data indicate that the crust is thickest under the south pole, thinning northward from the cratered terrain to the northern plains. Analysis of altimetry data demonstrates that Mars is “egg-shaped” with gravitational equipotential contours that show that channel systems in the southern highlands drained to the north, largely to the Chryse trough. A closed contour in the northern plains is consistent with the existence of a great northern ocean. Emission spectra of low-albedo regions show that basaltic rocks dominate spectral signatures on the southern highlands, whereas basaltic andesites dominate the northern lowlands. The bright regions show nondiagnostic spectra, similar to that of dust in the atmosphere. Signatures of aqueous minerals (e.g., clays, carbonates, and sulfates) are noticeably absent from the emission spectra. High spatial resolution images show that the surface has been extensively modified by wind and that layering is nearly ubiquitous, implying that a complex history of events is recorded in surface and near-surface materials. Altimetry data imply that both permanent caps are composed of water ice and dust, with seasonal covers of carbon dioxide frost. Finally, the altimetry data, coupled with thousands of atmospheric profiles, are providing new boundary conditions and dynamic controls for the generation and testing of more realistic dynamic models of the global circulation of the atmosphere.
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Mars global Surveyor mission: overview and status.
Science (New York N.Y.), 1998Co-Authors: Arden L. Albee, Frank D. Palluconi, Raymond E. ArvidsonAbstract:The Mars Global Surveyor (MGS) spacecraft achieved a 45-hour elliptical orbit at Mars on 11 September 1997 after an 11-month cruise from Earth. The mission is acquiring high-quality global observations of the martian surface and atmosphere and of its magnetic and gravitational fields. These observations will continue for one martian year.
