The Experts below are selected from a list of 246 Experts worldwide ranked by ideXlab platform
Monica Jain - One of the best experts on this subject based on the ideXlab platform.
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Total dystrophic onychomycosis due to Syncephalastrum racemosum – A rare cause and its novel treatment option
Wolters Kluwer Medknow Publications, 2019Co-Authors: Prasenjeet Mohanty, Siddhartha Dash, Liza Mohapatra, Monica JainAbstract:Onychomycosis is a fungal infection of the nail caused by dermatophytic (99%) and/or non-dermatophytic (1%) (including yeasts) infections of the nailplate. Among the non-dermatophytes, the yeast Candida albicans, Candida tropicalis, and other molds like Scopulariopsis spp., Scytalidium spp., Fusarium spp., and Aspergillus spp. may be responsible. Herein, we report a case of total dystrophic onychomycosis in a 41-year-old female, caused by Syncephalastrum racemosum and its complete improvement with a combination of oral pulse itraconazole and 1064 nm Q-switched Nd-YAG laser. This case is being reported due to the rarity of causative organism for onychomycosis and a novel approach in its treatment
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Total Dystrophic Onychomycosis Due to Syncephalastrum racemosum - A Rare Cause and its Novel Treatment Option.
Indian dermatology online journal, 2019Co-Authors: Prasenjeet Mohanty, Siddhartha Dash, Liza Mohapatra, Monica JainAbstract:Onychomycosis is a fungal infection of the nail caused by dermatophytic (99%) and/or non-dermatophytic (1%) (including yeasts) infections of the nailplate. Among the non-dermatophytes, the yeast Candida albicans, Candida tropicalis, and other molds like Scopulariopsis spp., Scytalidium spp., Fusarium spp., and Aspergillus spp. may be responsible. Herein, we report a case of total dystrophic onychomycosis in a 41-year-old female, caused by Syncephalastrum racemosum and its complete improvement with a combination of oral pulse itraconazole and 1064 nm Q-switched Nd-YAG laser. This case is being reported due to the rarity of causative organism for onychomycosis and a novel approach in its treatment.
Ta-hsiu Liao - One of the best experts on this subject based on the ideXlab platform.
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Protein structure and gene cloning of Syncephalastrum racemosum nuclease
Biochemical Journal, 1999Co-Authors: Ta-hsiu LiaoAbstract:The complete amino acid sequence of the fungus Syncephalastrum racemosum (Sr-) nuclease has been delineated on the basis of protein sequencing of the intact protein and its protease-digested peptides. The resulting 250-residue sequence shows a carbohydrate side chain attached at Asn134 and two half-cystine residues (Cys242 and Cys247) cross-linked to form a small disulphide loop. On the basis of the sequence of Sr-nuclease, a computer search in the sequence database yielded 60% and 48% positional identities with the sequences of Cunninghamella echinulata nuclease C1 and yeast mitochondria nuclease respectively, and very little similarity to those of several known mammalian DNases I. Sequence alignment of the three similar nucleases reveals that the single small disulphide loop is unchanged but the carbohydrate attachment in Sr-nuclease is absent from the other two nucleases. Alignment also shows a highly conserved region harbouring Sr-nuclease His85, which is assigned as one of the essential residues in the active site. The cDNA encoding Sr-nuclease was amplified by using reverse transcriptase-mediated PCR with degenerate primers based on its amino acid sequence. Subsequently, specific primers were synthesized for use in the 3' and 5' rapid amplification of cDNA ends (RACE). Direct sequencing of the RACE products led to the deduction of a 1.1 kb cDNA sequence for Sr-nuclease. The cDNA contains an open reading frame of 320 amino acid residues including a 70-residue putative signal peptide and the 250-residue mature protein. Finally, the recombinant Sr-nuclease was expressed in Escherichia coli strain BL21(DE3) in which the recombinant protein, after solubilization with detergent and renaturation, showed both DNase and RNase activities. The assignment of His85 to the active site was further supported by evidence that the mutant protein Sr-nuclease (H85A), in which His85 was replaced by Ala, was not able to degrade DNA or RNA.
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Deoxyribonuclease of Syncephalastrum racemosum--enzymatic properties and molecular structure.
Archives of biochemistry and biophysics, 1993Co-Authors: Ling-yun Chen, Ying-chieh Tsai, Ta-hsiu LiaoAbstract:Among the isolated fungal species of soil, one filamentous fungus, Syncephalastrum racemosum, produces a relatively large amount of DNase. This enzyme has been purified to apparent homogeneity by column chromatography on DEAE-cellulose, hydroxylapatite, phenyl-Sepharose CL-4B, and Sephadex G-100. The active enzyme requires divalent metal ions and has an optimum pH of 7.0 with Mg2+ and 7.2 with Mn2+. This enzyme is an acidic glycoprotein with a pI 5.0 and is relatively unstable at low concentrations. The M(r) of the enzyme is 56,000 during gel filtration under nondenaturing conditions but is 28,000 during polyacrylamide gel electrophoresis in sodium dodecyl sulfate. These results suggest a structure consisting of two subunits. The subunits of the holoenzyme can be cross-linked with glutaraldehyde. The yield of N-terminal phenylthiohydantoin-alanine from the holoenzyme is 140% and that of one peptide (D-Y-V-S-S-G-Y-D-R), obtained from the tryptic digest is 160%, indicating that the native enzyme is composed of two identical subunits and probably has two active domains. Fungal DNase can be inactivated by Cu(2+)-iodoacetate under conditions that inactivate bovine pancreatic DNase. The specific activity (units/mg of protein) of fungal DNase is 6.5 times that of bovine DNase. The amino acid content of fungal DNase, relative to bovine DNase, is higher in Gly and lower in Ser and Val. The fungal N-terminal 40-residue sequence shows a high degree of homology with a consensus sequence derived from DNase of three mammalian species.
Giovanna Cristina Varese - One of the best experts on this subject based on the ideXlab platform.
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Biocatalysed reduction of carboxylic acids to primary alcohols in aqueous medium: A novel synthetic capability of the zygomycete fungus Syncephalastrum racemosum
Journal of Molecular Catalysis B: Enzymatic, 2015Co-Authors: Elisabetta Brenna, Flavia Cannavale, Michele Crotti, Fabio Parmeggiani, Alice Romagnolo, Federica Spina, Giovanna Cristina VareseAbstract:Abstract The zygomycete fungus Syncephalastrum racemosum shows the remarkable capability of reducing carboxylic acids to primary alcohols in water medium, at ambient temperature and pressure. The reaction does not require molecular hydrogen, and in most cases affords quantitative transformations. The results herein reported highlight the basic molecular scaffold that can be accepted by the fungus, the effects due to substituents, and also the possibility that carboxylic acids can be generated in the reaction medium by enzymatic hydrolysis of the corresponding methyl esters. This biocatalysed reduction implements the scarcely supplied enzymatic toolbox for the conversion of carboxylic groups into primary alcohols, which can be exploited for the optimisation of sustainable synthetic procedures.
Rekha Bhadauria - One of the best experts on this subject based on the ideXlab platform.
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Fungal and Mycotoxin Contamination of Some Common Stored Herbal Fruit Samples
The Journal of Indian Botanical Society, 2010Co-Authors: Ajay Kumar Gautam, Rekha BhadauriaAbstract:A total of 106 stored dried fruit samples ofEmblica officinalis (Alma), Terminalia bellerica (Baheda) and Terminaliachebula (Haritiki), randomly collected from various retailers of Gwalior city, Madhya Pradesh (India), were analyzed for mould and mycotoxins contamination. Mycological examination revealed that 97.77% of the total samples examined, were found to be contaminated with different fungi. A total of 17 different fungal species were isolated from all the fruit samples. The predominant mycoflora obtained was distributed in five different genera comprising ofAspergillus, Penicillium, Alternaria, Rhizopus and Syncephalastrum. The Aspergillus (71.95%) was recovered as the most dominant genus followed by Penicillium (15.44%), Rhizopus (9.51%), A lternaria (1.67%) and Syncephalastrum (1.41%). During mycotoxicological investigation of fruit samples six mycotoxins namely aflatoxin B1 & B2 aflatoxin G1 & G2, citrinin and sterigmatocystin were detected. Aflatoxin B was detected as an important contaminant (34.43%) in the tested fruit samples followed by sterigmatocystin (17.54%) and aflatoxin B1 (12.88%). Alatoxin G1 and G2 are detected in 9.22% and 11.10% samples, respectively whereas; citrinin was from 3.07% samples only. Aflatoxin B2 was detected maximum in 65.51% baheda fruits, 22% haritiki fruits and in 15.8% amla fruits only. The presence of wide range of fungi and their mycotoxins in the fruits of E. officinalis, T. bellerica and T. chebula showed the potential risk of the use of these herbal fruits and their products for the users.
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Fungal Contamination Of few Common Stored herbal Fruit Samples
The Internet Journal of Nutrition and Wellness, 2008Co-Authors: Ajay Kumar Gautam, Rekha BhadauriaAbstract:Abstract The levels of fungal contamination were analyzed in 44 stored dried fruit samples of Emblica officinalis (Alma), Terminalia bellirica (Baheda) and Treminalia chebula (Haritiki), randomly collected from various retailers from Gwalior city (India). As a result of mycological examination, 97.77% of the total samples examined, found to be contaminated with different fungi. A total of 12 different fungal species were isolated from all the three types of fruit samples of Emblica officinalis, Terminalia bellirica and Terminalia chebula . The predominant mycoflora obtained was distributed in five different genera comprised of Aspergillus, Penicillium, Alternaria, Rhizopus and Syncephalastrum. The Aspergillus (71.95%) was observed as the most dominant genera recovered followed by Penicillium (15.44%), Rhizopus (9.51%) , Alternaria (1.67%) and Syncephalastrum(1.41%). Most of the identified fungal species like Aspergillus, Penicillium and Alternaria are reported to have ability to produce mycotoxins like aflatoxins, ochratoxins, citrinine and alternaria toxins. The presence of a wide range of storage fungi indicates that the mould probably infects the crude herbal drugs during harvesting and post harvesting processing i.e. mainly during drying, storing, transportation and processing. On the basis of present investigations, it may be concluded that the contamination of raw materials is alarming, as these raw materials needs thorough
Prasenjeet Mohanty - One of the best experts on this subject based on the ideXlab platform.
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Total dystrophic onychomycosis due to Syncephalastrum racemosum – A rare cause and its novel treatment option
Wolters Kluwer Medknow Publications, 2019Co-Authors: Prasenjeet Mohanty, Siddhartha Dash, Liza Mohapatra, Monica JainAbstract:Onychomycosis is a fungal infection of the nail caused by dermatophytic (99%) and/or non-dermatophytic (1%) (including yeasts) infections of the nailplate. Among the non-dermatophytes, the yeast Candida albicans, Candida tropicalis, and other molds like Scopulariopsis spp., Scytalidium spp., Fusarium spp., and Aspergillus spp. may be responsible. Herein, we report a case of total dystrophic onychomycosis in a 41-year-old female, caused by Syncephalastrum racemosum and its complete improvement with a combination of oral pulse itraconazole and 1064 nm Q-switched Nd-YAG laser. This case is being reported due to the rarity of causative organism for onychomycosis and a novel approach in its treatment
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Total Dystrophic Onychomycosis Due to Syncephalastrum racemosum - A Rare Cause and its Novel Treatment Option.
Indian dermatology online journal, 2019Co-Authors: Prasenjeet Mohanty, Siddhartha Dash, Liza Mohapatra, Monica JainAbstract:Onychomycosis is a fungal infection of the nail caused by dermatophytic (99%) and/or non-dermatophytic (1%) (including yeasts) infections of the nailplate. Among the non-dermatophytes, the yeast Candida albicans, Candida tropicalis, and other molds like Scopulariopsis spp., Scytalidium spp., Fusarium spp., and Aspergillus spp. may be responsible. Herein, we report a case of total dystrophic onychomycosis in a 41-year-old female, caused by Syncephalastrum racemosum and its complete improvement with a combination of oral pulse itraconazole and 1064 nm Q-switched Nd-YAG laser. This case is being reported due to the rarity of causative organism for onychomycosis and a novel approach in its treatment.
