The Experts below are selected from a list of 516 Experts worldwide ranked by ideXlab platform
Lei Ming - One of the best experts on this subject based on the ideXlab platform.
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Effects of SIRT1 gene knock-out via activation of SREBP2 protein-mediated PI3K/AKT signaling on osteoarthritis in mice
JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES, 2016Co-Authors: Yu Fei, Li Wei, Xiao De-ming, Lei Ming, Zeng Hui, Yuan Hao, Lin Jian-jingAbstract:This study investigated the effects of SIRT1 gene knock-out on osteoarthritis in mice, and the possible roles of SREBP2 protein and the PI3K/AKT signaling pathway in the effects. Mice were randomly divided into a normal group and a SIRT1 gene knock-out group (6 mice in each group). In these groups, one side of the knee anterior cruciate ligament was traversed, and the ipsilateral medial meniscus was cut to establish an osteoarthritis model of knee joint. The countralateral Synovial Bursa was cut out, serving as controls. The knee joint specimens were then divided into four groups: SIRT1(+/+) control group (group A, n=6); SIRT1(+/+) osteoarthritis group (group B, n=6); SIRT1(-/-) control group (group C, n=6); SIRT1(-/-) osteoarthritis group (group D, n=6). HE staining, Masson staining, Safranin O-Fast Green staining and Van Gieson staining were used to observe the morphological changes in the articular cartilage of the knee. Immunohistochemical staining was employed to detect the expression of SIRT1, SREBP2, VEGF, AKT, HMGCR and type II collagen proteins. SA-beta-gal staining was utilized to evaluate chondrocyte aging. The results showed clear knee joint cartilage destruction and degeneration in the SIRT1(-/-) osteoarthritis group. The tidal line was twisted and displaced anteriorly. Type II collagen was destroyed and distributed unevenly. Compared with the SIRT1(+/+) osteoarthritis group and SIRT1(-/-) control group, SIRT1 protein expression was not obviously changed in the SIRT1(-/-) osteoarthritis group (P > 0.05), while the expression levels of the SREBP2, VEGF and HMGCR proteins were significantly increased (P < 0.05) and the levels of AKT and type II collagen proteins were significantly decreased (P < 0.05). SIRT1 gene knock-out may aggravate cartilage degeneration in osteoarthritis by activating the SREBP2 protein-mediated PI3K/AKT signalling pathway, suggesting that SIRT1 gene may play a protective role against osteoarthritis.National Natural Science Foundation of China [81272032]; Research and Development Projects of Shenzhen of China [JCYJ20150403091443275]SCI(E)PubMedARTICLEoscarfyu@163.com; zenghui_36@163.com5683-6903
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Arthroscopic treatment of bony loose bodies in the subacromial space
international journal of surgery case reports, 2015Co-Authors: Li Wei, Xiao De-ming, Jiang Chang-qing, Zhang Wen-tao, Lei MingAbstract:Multiple bony loose bodies in the subacromial space caused form cartilage or bone cells and continue to grow.A 58-year-old man with two-year history of swelling and pain of the right shoulder. He had no history of tuberculosis and rheumatoid arthritis. Magnetic resonance (MR) images showed some bony loose bodies in the subacromial space. The removal of loose bodies and Bursa debridement were performed arthroscopically. Histological diagnosis of them was synovitis with fibrous bodies.Extra-articular loose bodies is extremely rare, especially in the subacromial space, which maybe originated in the proliferative Synovial Bursa. Most authors recommend open removal to relive the pain, but there were choice to apply arthroscopy to remove them.The mechanism of formation of bony loose bodies is not clear, may be associated with Synovial cartilage metaplasia. Arthroscopic removal of loose bodies and Bursa debridement is a good option for treatment of the loose body in the subacromial space, which can receive good function.Copyright ? 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000361071700028&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701PubMed0101-1031
Ming Lei - One of the best experts on this subject based on the ideXlab platform.
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Arthroscopic treatment of bony loose bodies in the subacromial space
Elsevier, 2015Co-Authors: De-ming Xiao, Chang-qing Jiang, Wen-tao Zhang, Ming LeiAbstract:Introduction: Multiple bony loose bodies in the subacromial space caused form cartilage or bone cells and continue to grow. Presentation of case: A 58-year-old man with two-year history of swelling and pain of the right shoulder. He had no history of tuberculosis and rheumatoid arthritis. Magnetic resonance (MR) images showed some bony loose bodies in the subacromial space. The removal of loose bodies and Bursa debridement were performed arthroscopically. Histological diagnosis of them was synovitis with fibrous bodies. Discussion: Extra-articular loose bodies is extremely rare, especially in the subacromial space, which maybe originated in the proliferative Synovial Bursa. Most authors recommend open removal to relive the pain, but there were choice to apply arthroscopy to remove them. Conclusion: The mechanism of formation of bony loose bodies is not clear, may be associated with Synovial cartilage metaplasia. Arthroscopic removal of loose bodies and Bursa debridement is a good option for treatment of the loose body in the subacromial space, which can receive good function
Li Wei - One of the best experts on this subject based on the ideXlab platform.
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Effects of SIRT1 gene knock-out via activation of SREBP2 protein-mediated PI3K/AKT signaling on osteoarthritis in mice
JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES, 2016Co-Authors: Yu Fei, Li Wei, Xiao De-ming, Lei Ming, Zeng Hui, Yuan Hao, Lin Jian-jingAbstract:This study investigated the effects of SIRT1 gene knock-out on osteoarthritis in mice, and the possible roles of SREBP2 protein and the PI3K/AKT signaling pathway in the effects. Mice were randomly divided into a normal group and a SIRT1 gene knock-out group (6 mice in each group). In these groups, one side of the knee anterior cruciate ligament was traversed, and the ipsilateral medial meniscus was cut to establish an osteoarthritis model of knee joint. The countralateral Synovial Bursa was cut out, serving as controls. The knee joint specimens were then divided into four groups: SIRT1(+/+) control group (group A, n=6); SIRT1(+/+) osteoarthritis group (group B, n=6); SIRT1(-/-) control group (group C, n=6); SIRT1(-/-) osteoarthritis group (group D, n=6). HE staining, Masson staining, Safranin O-Fast Green staining and Van Gieson staining were used to observe the morphological changes in the articular cartilage of the knee. Immunohistochemical staining was employed to detect the expression of SIRT1, SREBP2, VEGF, AKT, HMGCR and type II collagen proteins. SA-beta-gal staining was utilized to evaluate chondrocyte aging. The results showed clear knee joint cartilage destruction and degeneration in the SIRT1(-/-) osteoarthritis group. The tidal line was twisted and displaced anteriorly. Type II collagen was destroyed and distributed unevenly. Compared with the SIRT1(+/+) osteoarthritis group and SIRT1(-/-) control group, SIRT1 protein expression was not obviously changed in the SIRT1(-/-) osteoarthritis group (P > 0.05), while the expression levels of the SREBP2, VEGF and HMGCR proteins were significantly increased (P < 0.05) and the levels of AKT and type II collagen proteins were significantly decreased (P < 0.05). SIRT1 gene knock-out may aggravate cartilage degeneration in osteoarthritis by activating the SREBP2 protein-mediated PI3K/AKT signalling pathway, suggesting that SIRT1 gene may play a protective role against osteoarthritis.National Natural Science Foundation of China [81272032]; Research and Development Projects of Shenzhen of China [JCYJ20150403091443275]SCI(E)PubMedARTICLEoscarfyu@163.com; zenghui_36@163.com5683-6903
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Arthroscopic treatment of bony loose bodies in the subacromial space
international journal of surgery case reports, 2015Co-Authors: Li Wei, Xiao De-ming, Jiang Chang-qing, Zhang Wen-tao, Lei MingAbstract:Multiple bony loose bodies in the subacromial space caused form cartilage or bone cells and continue to grow.A 58-year-old man with two-year history of swelling and pain of the right shoulder. He had no history of tuberculosis and rheumatoid arthritis. Magnetic resonance (MR) images showed some bony loose bodies in the subacromial space. The removal of loose bodies and Bursa debridement were performed arthroscopically. Histological diagnosis of them was synovitis with fibrous bodies.Extra-articular loose bodies is extremely rare, especially in the subacromial space, which maybe originated in the proliferative Synovial Bursa. Most authors recommend open removal to relive the pain, but there were choice to apply arthroscopy to remove them.The mechanism of formation of bony loose bodies is not clear, may be associated with Synovial cartilage metaplasia. Arthroscopic removal of loose bodies and Bursa debridement is a good option for treatment of the loose body in the subacromial space, which can receive good function.Copyright ? 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000361071700028&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701PubMed0101-1031
Xiao De-ming - One of the best experts on this subject based on the ideXlab platform.
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Effects of SIRT1 gene knock-out via activation of SREBP2 protein-mediated PI3K/AKT signaling on osteoarthritis in mice
JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES, 2016Co-Authors: Yu Fei, Li Wei, Xiao De-ming, Lei Ming, Zeng Hui, Yuan Hao, Lin Jian-jingAbstract:This study investigated the effects of SIRT1 gene knock-out on osteoarthritis in mice, and the possible roles of SREBP2 protein and the PI3K/AKT signaling pathway in the effects. Mice were randomly divided into a normal group and a SIRT1 gene knock-out group (6 mice in each group). In these groups, one side of the knee anterior cruciate ligament was traversed, and the ipsilateral medial meniscus was cut to establish an osteoarthritis model of knee joint. The countralateral Synovial Bursa was cut out, serving as controls. The knee joint specimens were then divided into four groups: SIRT1(+/+) control group (group A, n=6); SIRT1(+/+) osteoarthritis group (group B, n=6); SIRT1(-/-) control group (group C, n=6); SIRT1(-/-) osteoarthritis group (group D, n=6). HE staining, Masson staining, Safranin O-Fast Green staining and Van Gieson staining were used to observe the morphological changes in the articular cartilage of the knee. Immunohistochemical staining was employed to detect the expression of SIRT1, SREBP2, VEGF, AKT, HMGCR and type II collagen proteins. SA-beta-gal staining was utilized to evaluate chondrocyte aging. The results showed clear knee joint cartilage destruction and degeneration in the SIRT1(-/-) osteoarthritis group. The tidal line was twisted and displaced anteriorly. Type II collagen was destroyed and distributed unevenly. Compared with the SIRT1(+/+) osteoarthritis group and SIRT1(-/-) control group, SIRT1 protein expression was not obviously changed in the SIRT1(-/-) osteoarthritis group (P > 0.05), while the expression levels of the SREBP2, VEGF and HMGCR proteins were significantly increased (P < 0.05) and the levels of AKT and type II collagen proteins were significantly decreased (P < 0.05). SIRT1 gene knock-out may aggravate cartilage degeneration in osteoarthritis by activating the SREBP2 protein-mediated PI3K/AKT signalling pathway, suggesting that SIRT1 gene may play a protective role against osteoarthritis.National Natural Science Foundation of China [81272032]; Research and Development Projects of Shenzhen of China [JCYJ20150403091443275]SCI(E)PubMedARTICLEoscarfyu@163.com; zenghui_36@163.com5683-6903
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Arthroscopic treatment of bony loose bodies in the subacromial space
international journal of surgery case reports, 2015Co-Authors: Li Wei, Xiao De-ming, Jiang Chang-qing, Zhang Wen-tao, Lei MingAbstract:Multiple bony loose bodies in the subacromial space caused form cartilage or bone cells and continue to grow.A 58-year-old man with two-year history of swelling and pain of the right shoulder. He had no history of tuberculosis and rheumatoid arthritis. Magnetic resonance (MR) images showed some bony loose bodies in the subacromial space. The removal of loose bodies and Bursa debridement were performed arthroscopically. Histological diagnosis of them was synovitis with fibrous bodies.Extra-articular loose bodies is extremely rare, especially in the subacromial space, which maybe originated in the proliferative Synovial Bursa. Most authors recommend open removal to relive the pain, but there were choice to apply arthroscopy to remove them.The mechanism of formation of bony loose bodies is not clear, may be associated with Synovial cartilage metaplasia. Arthroscopic removal of loose bodies and Bursa debridement is a good option for treatment of the loose body in the subacromial space, which can receive good function.Copyright ? 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000361071700028&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701PubMed0101-1031
De-ming Xiao - One of the best experts on this subject based on the ideXlab platform.
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Arthroscopic treatment of bony loose bodies in the subacromial space
Elsevier, 2015Co-Authors: De-ming Xiao, Chang-qing Jiang, Wen-tao Zhang, Ming LeiAbstract:Introduction: Multiple bony loose bodies in the subacromial space caused form cartilage or bone cells and continue to grow. Presentation of case: A 58-year-old man with two-year history of swelling and pain of the right shoulder. He had no history of tuberculosis and rheumatoid arthritis. Magnetic resonance (MR) images showed some bony loose bodies in the subacromial space. The removal of loose bodies and Bursa debridement were performed arthroscopically. Histological diagnosis of them was synovitis with fibrous bodies. Discussion: Extra-articular loose bodies is extremely rare, especially in the subacromial space, which maybe originated in the proliferative Synovial Bursa. Most authors recommend open removal to relive the pain, but there were choice to apply arthroscopy to remove them. Conclusion: The mechanism of formation of bony loose bodies is not clear, may be associated with Synovial cartilage metaplasia. Arthroscopic removal of loose bodies and Bursa debridement is a good option for treatment of the loose body in the subacromial space, which can receive good function
