The Experts below are selected from a list of 2658 Experts worldwide ranked by ideXlab platform
Otto Braun-falco - One of the best experts on this subject based on the ideXlab platform.
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Differences in the skin surface pH and bacterial microflora due to the long-term application of Synthetic Detergent preparations of pH 5.5 and pH 7.0. Results of a crossover trial in healthy volunteers.
Acta dermato-venereologica, 1990Co-Authors: Hans Christian Korting, K. Hübner, K. Greiner, G. Hamm, Otto Braun-falcoAbstract:Skin cleansing preparations consisting of identical Synthetic Detergents but differing in pH-value (pH 5.5 and 7.0) were applied twice daily on the forehead and forearm of healthy volunteers in a randomized crossover trial. The skin surface pH was found to be significantly higher when the neutral preparation had been used, as was the propionibacterial count (p less than 0.05). The number of propionibacteria was significantly linked to the skin pH. Hence even minor differences in the pH of skin cleansing preparations seem to be of importance for the integrity of the skin surface. This should be taken into account when planning the formulation of optimal skin care products.
Wakako Tsuzuki - One of the best experts on this subject based on the ideXlab platform.
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Use of a Synthetic Detergent to partition protein mixtures
The Analyst, 1993Co-Authors: Wakako Tsuzuki, Hanae Kasumimoto, Shouichi. KobayashiAbstract:A previously reported method for the preparation of organic solvent soluble lipase in high yield has been applied to the partitioning of a protein mixture using a mixture of six known proteins as a model system. The organic solvent soluble complex of these proteins was obtained according to the previously reported method. In order to extract the proteins from the complex, the latter was dissolved in dichloromethane followed by the addition of buffer and triethylamine. By using this procedure, the proteins could be recovered from the complex formed with the Detergent. It was found that the composition of the solvent used to prepare the complex influenced the ability of each protein to form a complex with the Detergent. By making use of the differences in the efficiency of each protein to form a complex, several crude lipases could be successfully purified; in addition, their activities were retained during the purification procedure. The results suggest that the application of a Synthetic Detergent may be effective for the purification of proteins and enzymes.
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Reactive properties of the organic solvent-soluble lipase
Biochimica et Biophysica Acta, 1991Co-Authors: Wakako Tsuzuki, Tateo SuzukiAbstract:Abstract In a previous report, the organic solvent-soluble lipase was prepared using a Synthetic Detergent, didodecyl glucosyl glutamate, and it was estimated that 150 ± 30 molecules of the Detergent were attached to one lipase molecule based on gel permeation chromatography and chemical analysis. In this paper, the reactivity of the organic solvent-soluble lipase was compared with that of the native lipase to study the effect of the surrounding Detergent on the thermostability and enzymatic reactivity. The activity of the organic solvent-soluble lipase was preserved in the organic solvents up to a temperature of 50°C as in the case of the native lipase in buffer (pH 7.0). The influence of the chain length of fatty acids of the substrate triacylglycerols on the hydrolysis activities was studied. The organic solvent-soluble lipase hydrolyzed triacylglycerols with longer chains more rapidly than the native lipase. The presence of Ca2+ at 0.1 mM stimulated the activity of the native lipase, whereas Ca2+ at a high concentration inhibited it. On the other hand, even at a low concentration, Ca2+ inhibited the activity of the organic solvent-soluble lipase. These results suggest that the Detergent attached to the lipase molecule affected the reactive properties.
Shouichi. Kobayashi - One of the best experts on this subject based on the ideXlab platform.
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Use of a Synthetic Detergent to partition protein mixtures
The Analyst, 1993Co-Authors: Wakako Tsuzuki, Hanae Kasumimoto, Shouichi. KobayashiAbstract:A previously reported method for the preparation of organic solvent soluble lipase in high yield has been applied to the partitioning of a protein mixture using a mixture of six known proteins as a model system. The organic solvent soluble complex of these proteins was obtained according to the previously reported method. In order to extract the proteins from the complex, the latter was dissolved in dichloromethane followed by the addition of buffer and triethylamine. By using this procedure, the proteins could be recovered from the complex formed with the Detergent. It was found that the composition of the solvent used to prepare the complex influenced the ability of each protein to form a complex with the Detergent. By making use of the differences in the efficiency of each protein to form a complex, several crude lipases could be successfully purified; in addition, their activities were retained during the purification procedure. The results suggest that the application of a Synthetic Detergent may be effective for the purification of proteins and enzymes.
Hans Christian Korting - One of the best experts on this subject based on the ideXlab platform.
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Differences in the skin surface pH and bacterial microflora due to the long-term application of Synthetic Detergent preparations of pH 5.5 and pH 7.0. Results of a crossover trial in healthy volunteers.
Acta dermato-venereologica, 1990Co-Authors: Hans Christian Korting, K. Hübner, K. Greiner, G. Hamm, Otto Braun-falcoAbstract:Skin cleansing preparations consisting of identical Synthetic Detergents but differing in pH-value (pH 5.5 and 7.0) were applied twice daily on the forehead and forearm of healthy volunteers in a randomized crossover trial. The skin surface pH was found to be significantly higher when the neutral preparation had been used, as was the propionibacterial count (p less than 0.05). The number of propionibacteria was significantly linked to the skin pH. Hence even minor differences in the pH of skin cleansing preparations seem to be of importance for the integrity of the skin surface. This should be taken into account when planning the formulation of optimal skin care products.
T S M Wan - One of the best experts on this subject based on the ideXlab platform.
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environmental fate and chemistry of organic pollutants in the sediment of xiamen and victoria harbours
Marine Pollution Bulletin, 1995Co-Authors: Hualong Hong, Luoping Zhang, J C Chen, Yuk Shan Wong, T S M WanAbstract:Abstract Sediment samples were collected from Xiamen Harbour, the People's Republic of China, and Victoria Harbour, Hong Kong, and were analysed for non-aromatic hydrocarbons (NAHs), long-chain linear alkylbenzenes (LABs), polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides (HCHs, DDTs) and polychlorinated biphenyls (PCBs). In Xiamen Harbour, concentrations varied from 3.1–32.9 μg g−1 for NAH, 2.9–61 μg g−1 for PAHs, 0.14–1.12 ng g−1 for HCHs, 4.45–311 ng g−1 for DDTs and 0.05–7.24 ng g−1 for PCBs. In Victoria Harbour, the concentration ranges of those pollutants were 60–646 μg g−1, 1.2–14.0 μg g−1, undetectable to 2.3 ng g−1, 1.38–30.3 ng g−1 and 3.2–16.0 ng g−1, respectively. The distribution patterns of various organic pollutants in the sediments are discussed. The NAH levels present in Victoria Harbour indicated that there was significant petroleum contamination in the harbour. Xiamen Harbour was slightly more contaminated by DDTs. LABs could be used as Synthetic Detergent tracers. From the data available, it is concluded that Victoria Harbour is more polluted than Xiamen Harbour. The structure of the benthic community provides further evidence to support this claim.
