The Experts below are selected from a list of 128544 Experts worldwide ranked by ideXlab platform
Yutaka Takebe - One of the best experts on this subject based on the ideXlab platform.
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novel 3α methoxyserrat 14 en 21β ol pj 1 and 3β methoxyserrat 14 en 21β ol pj 2 curcumin kojic acid quercetin and baicalein conjugates as hiv agents
Bioorganic & Medicinal Chemistry, 2009Co-Authors: Reiko Tanaka, Hiroko Tsujii, Takeshi Yamada, Tetsuya Kajimoto, Fumio Amano, Junya Hasegawa, Yoshio Hamashima, Manabu Node, Kayoko Katoh, Yutaka TakebeAbstract:Abstract Sixteen novel compounds; 3α-methoxyserrat-14-en-21β-ol ( 1 ) and 3β-methoxyserrat-14-en-21β-ol ( 2 ) and their curcumin, kojic acid, quercetin, and baicalein conjugates ( 3 )–( 18 ) were designed, synthesized, and evaluated for in vitro anti-HIV-1 reverse transcriptase (RT) activity in infected C8166-CCR5 Cells, a human CD4 + T-Lymphocyte Cell Line. Among them, kojic acid derivatives, 9–12 showed significant biological activity. In particular, the compound 13 , the conjugate of two molecules of 3α-methoxyserrat-14-en-21β-ol ( 1 ) and one molecule of kojic acid, exerted significant anti-HIV activity with an EC50 value of 0.12 μg/mL.
Ad A C M Peijnenburg - One of the best experts on this subject based on the ideXlab platform.
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transcriptome analysis of the human t lymphocyte Cell Line jurkat and human peripheral blood mononuclear Cells exposed to deoxynivalenol don new mechanistic insights
Toxicology and Applied Pharmacology, 2012Co-Authors: Madhumohan R Katika, Peter J M Hendriksen, Henk Van Loveren, Jia Shao, Ad A C M PeijnenburgAbstract:Deoxynivalenol (DON) or vomitoxin is a commonly encountered type-B trichothecene mycotoxin, produced by Fusarium species predominantly found in cereals and grains. DON is known to exert toxic effects on the gastrointestinal, reproductive and neuroendocrine systems, and particularly on the immune system. Depending on dose and exposure time, it can either stimulate or suppress immune function. The main objective of this study was to obtain a deeper insight into DON-induced effects on lymphoid Cells. For this, we exposed the human T-Lymphocyte Cell Line Jurkat and human peripheral blood mononuclear Cells (PBMCs) to various concentrations of DON for various times and examined gene expression changes by DNA microarray analysis. Jurkat Cells were exposed to 0.25 and 0.5 mu M DON for 3, 6 and 24 h. Biological interpretation of the microarray data indicated that DON affects various processes in these Cells: It upregulates genes involved in ribosome structure and function, RNA/protein synthesis and processing, endoplasmic reticulum (ER) stress, calcium-mediated signaling, mitochondrial function, oxidative stress, the NFAT and NF-kappa B/TNF-alpha pathways, T Cell activation and apoptosis. The effects of DON on the expression of genes involved in ER stress, NFAT activation and apoptosis were confirmed by qRT-PCR. Other biochemical experiments confirmed that DON activates calcium-dependent proteins such as calcineurin and M-calpain that are known to be involved in T Cell activation and apoptosis. Induction of T Cell activation was also confirmed by demonstrating that DON activates NFATC1 and induces its translocation from the cytoplasm to the nucleus. For the gene expression profiling of PBMCs, Cells were exposed to 2 and 4 mu M DON for 6 and 24 h. Comparison of the Jurkat microarray data with those obtained with PBMCs showed that most of the processes affected by DON in the Jurkat Cell Line were also affected in the PBMCs. (C) 2012 Elsevier Inc. All rights reserved.
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transcriptome analysis of the human t lymphocyte Cell Line jurkat and human peripheral blood mononuclear Cells exposed to deoxynivalenol don new mechanistic insights
Toxicology and Applied Pharmacology, 2012Co-Authors: Madhumohan R Katika, Peter J M Hendriksen, Jia Shao, Henk Van Loveren, Ad A C M PeijnenburgAbstract:Deoxynivalenol (DON) or vomitoxin is a commonly encountered type-B trichothecene mycotoxin, produced by Fusarium species predominantly found in cereals and grains. DON is known to exert toxic effects on the gastrointestinal, reproductive and neuroendocrine systems, and particularly on the immune system. Depending on dose and exposure time, it can either stimulate or suppress immune function. The main objective of this study was to obtain a deeper insight into DON-induced effects on lymphoid Cells. For this, we exposed the human T-Lymphocyte Cell Line Jurkat and human peripheral blood mononuclear Cells (PBMCs) to various concentrations of DON for various times and examined gene expression changes by DNA microarray analysis. Jurkat Cells were exposed to 0.25 and 0.5μM DON for 3, 6 and 24h. Biological interpretation of the microarray data indicated that DON affects various processes in these Cells: It upregulates genes involved in ribosome structure and function, RNA/protein synthesis and processing, endoplasmic reticulum (ER) stress, calcium-mediated signaling, mitochondrial function, oxidative stress, the NFAT and NF-κB/TNF-α pathways, T Cell activation and apoptosis. The effects of DON on the expression of genes involved in ER stress, NFAT activation and apoptosis were confirmed by qRT-PCR. Other biochemical experiments confirmed that DON activates calcium-dependent proteins such as calcineurin and M-calpain that are known to be involved in T Cell activation and apoptosis. Induction of T Cell activation was also confirmed by demonstrating that DON activates NFATC1 and induces its translocation from the cytoplasm to the nucleus. For the gene expression profiling of PBMCs, Cells were exposed to 2 and 4μM DON for 6 and 24h. Comparison of the Jurkat microarray data with those obtained with PBMCs showed that most of the processes affected by DON in the Jurkat Cell Line were also affected in the PBMCs.
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exposure of jurkat Cells to bis tri n butyltin oxide tbto induces transcriptomics changes indicative for er and oxidative stress t Cell activation and apoptosis
Toxicology and Applied Pharmacology, 2011Co-Authors: Madhumohan R Katika, Peter J M Hendriksen, Henk Van Loveren, Ad A C M PeijnenburgAbstract:Abstract Tributyltin oxide (TBTO) is an organotin compound that is widely used as a biocide in agriculture and as an antifouling agent in paints. TBTO is toxic for many Cell types, particularly immune Cells. The present study aimed to identify the effects of TBTO on the human T lymphocyte Cell Line Jurkat. Cells were treated with 0.2 and 0.5 μM TBTO for 3, 6, 12 and 24 h and then subjected to whole genome gene expression microarray analysis. The biological interpretation of the gene expression profiles revealed that endoplasmic reticulum (ER) stress is among the earliest effects of TBTO. Simultaneously or shortly thereafter, oxidative stress, activation of NFKB and NFAT, T Cell activation, and apoptosis are induced. The effects of TBTO on genes involved in ER stress, NFAT pathway, T Cell activation and apoptosis were confirmed by qRT-PCR. Activation and nuclear translocation of NFATC1 and the oxidative stress response proteins NRF2 and KEAP1 were confirmed by immunocytology. Taking advantage of previously published microarray data, we demonstrated that the induction of ER stress, oxidative stress, T Cell activation and apoptosis by TBTO is not unique for Jurkat Cells but does also occur in mouse thymocytes both ex vivo and in vivo and rat thymocytes ex vivo . We propose that the induction of ER stress leading to a T Cell activation response is a major factor in the higher sensitivity of immune Cells above other types of Cells for TBTO.
Reiko Tanaka - One of the best experts on this subject based on the ideXlab platform.
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novel 3α methoxyserrat 14 en 21β ol pj 1 and 3β methoxyserrat 14 en 21β ol pj 2 curcumin kojic acid quercetin and baicalein conjugates as hiv agents
Bioorganic & Medicinal Chemistry, 2009Co-Authors: Reiko Tanaka, Hiroko Tsujii, Takeshi Yamada, Tetsuya Kajimoto, Fumio Amano, Junya Hasegawa, Yoshio Hamashima, Manabu Node, Kayoko Katoh, Yutaka TakebeAbstract:Abstract Sixteen novel compounds; 3α-methoxyserrat-14-en-21β-ol ( 1 ) and 3β-methoxyserrat-14-en-21β-ol ( 2 ) and their curcumin, kojic acid, quercetin, and baicalein conjugates ( 3 )–( 18 ) were designed, synthesized, and evaluated for in vitro anti-HIV-1 reverse transcriptase (RT) activity in infected C8166-CCR5 Cells, a human CD4 + T-Lymphocyte Cell Line. Among them, kojic acid derivatives, 9–12 showed significant biological activity. In particular, the compound 13 , the conjugate of two molecules of 3α-methoxyserrat-14-en-21β-ol ( 1 ) and one molecule of kojic acid, exerted significant anti-HIV activity with an EC50 value of 0.12 μg/mL.
Madhumohan R Katika - One of the best experts on this subject based on the ideXlab platform.
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transcriptome analysis of the human t lymphocyte Cell Line jurkat and human peripheral blood mononuclear Cells exposed to deoxynivalenol don new mechanistic insights
Toxicology and Applied Pharmacology, 2012Co-Authors: Madhumohan R Katika, Peter J M Hendriksen, Henk Van Loveren, Jia Shao, Ad A C M PeijnenburgAbstract:Deoxynivalenol (DON) or vomitoxin is a commonly encountered type-B trichothecene mycotoxin, produced by Fusarium species predominantly found in cereals and grains. DON is known to exert toxic effects on the gastrointestinal, reproductive and neuroendocrine systems, and particularly on the immune system. Depending on dose and exposure time, it can either stimulate or suppress immune function. The main objective of this study was to obtain a deeper insight into DON-induced effects on lymphoid Cells. For this, we exposed the human T-Lymphocyte Cell Line Jurkat and human peripheral blood mononuclear Cells (PBMCs) to various concentrations of DON for various times and examined gene expression changes by DNA microarray analysis. Jurkat Cells were exposed to 0.25 and 0.5 mu M DON for 3, 6 and 24 h. Biological interpretation of the microarray data indicated that DON affects various processes in these Cells: It upregulates genes involved in ribosome structure and function, RNA/protein synthesis and processing, endoplasmic reticulum (ER) stress, calcium-mediated signaling, mitochondrial function, oxidative stress, the NFAT and NF-kappa B/TNF-alpha pathways, T Cell activation and apoptosis. The effects of DON on the expression of genes involved in ER stress, NFAT activation and apoptosis were confirmed by qRT-PCR. Other biochemical experiments confirmed that DON activates calcium-dependent proteins such as calcineurin and M-calpain that are known to be involved in T Cell activation and apoptosis. Induction of T Cell activation was also confirmed by demonstrating that DON activates NFATC1 and induces its translocation from the cytoplasm to the nucleus. For the gene expression profiling of PBMCs, Cells were exposed to 2 and 4 mu M DON for 6 and 24 h. Comparison of the Jurkat microarray data with those obtained with PBMCs showed that most of the processes affected by DON in the Jurkat Cell Line were also affected in the PBMCs. (C) 2012 Elsevier Inc. All rights reserved.
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transcriptome analysis of the human t lymphocyte Cell Line jurkat and human peripheral blood mononuclear Cells exposed to deoxynivalenol don new mechanistic insights
Toxicology and Applied Pharmacology, 2012Co-Authors: Madhumohan R Katika, Peter J M Hendriksen, Jia Shao, Henk Van Loveren, Ad A C M PeijnenburgAbstract:Deoxynivalenol (DON) or vomitoxin is a commonly encountered type-B trichothecene mycotoxin, produced by Fusarium species predominantly found in cereals and grains. DON is known to exert toxic effects on the gastrointestinal, reproductive and neuroendocrine systems, and particularly on the immune system. Depending on dose and exposure time, it can either stimulate or suppress immune function. The main objective of this study was to obtain a deeper insight into DON-induced effects on lymphoid Cells. For this, we exposed the human T-Lymphocyte Cell Line Jurkat and human peripheral blood mononuclear Cells (PBMCs) to various concentrations of DON for various times and examined gene expression changes by DNA microarray analysis. Jurkat Cells were exposed to 0.25 and 0.5μM DON for 3, 6 and 24h. Biological interpretation of the microarray data indicated that DON affects various processes in these Cells: It upregulates genes involved in ribosome structure and function, RNA/protein synthesis and processing, endoplasmic reticulum (ER) stress, calcium-mediated signaling, mitochondrial function, oxidative stress, the NFAT and NF-κB/TNF-α pathways, T Cell activation and apoptosis. The effects of DON on the expression of genes involved in ER stress, NFAT activation and apoptosis were confirmed by qRT-PCR. Other biochemical experiments confirmed that DON activates calcium-dependent proteins such as calcineurin and M-calpain that are known to be involved in T Cell activation and apoptosis. Induction of T Cell activation was also confirmed by demonstrating that DON activates NFATC1 and induces its translocation from the cytoplasm to the nucleus. For the gene expression profiling of PBMCs, Cells were exposed to 2 and 4μM DON for 6 and 24h. Comparison of the Jurkat microarray data with those obtained with PBMCs showed that most of the processes affected by DON in the Jurkat Cell Line were also affected in the PBMCs.
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exposure of jurkat Cells to bis tri n butyltin oxide tbto induces transcriptomics changes indicative for er and oxidative stress t Cell activation and apoptosis
Toxicology and Applied Pharmacology, 2011Co-Authors: Madhumohan R Katika, Peter J M Hendriksen, Henk Van Loveren, Ad A C M PeijnenburgAbstract:Abstract Tributyltin oxide (TBTO) is an organotin compound that is widely used as a biocide in agriculture and as an antifouling agent in paints. TBTO is toxic for many Cell types, particularly immune Cells. The present study aimed to identify the effects of TBTO on the human T lymphocyte Cell Line Jurkat. Cells were treated with 0.2 and 0.5 μM TBTO for 3, 6, 12 and 24 h and then subjected to whole genome gene expression microarray analysis. The biological interpretation of the gene expression profiles revealed that endoplasmic reticulum (ER) stress is among the earliest effects of TBTO. Simultaneously or shortly thereafter, oxidative stress, activation of NFKB and NFAT, T Cell activation, and apoptosis are induced. The effects of TBTO on genes involved in ER stress, NFAT pathway, T Cell activation and apoptosis were confirmed by qRT-PCR. Activation and nuclear translocation of NFATC1 and the oxidative stress response proteins NRF2 and KEAP1 were confirmed by immunocytology. Taking advantage of previously published microarray data, we demonstrated that the induction of ER stress, oxidative stress, T Cell activation and apoptosis by TBTO is not unique for Jurkat Cells but does also occur in mouse thymocytes both ex vivo and in vivo and rat thymocytes ex vivo . We propose that the induction of ER stress leading to a T Cell activation response is a major factor in the higher sensitivity of immune Cells above other types of Cells for TBTO.
Jia Shao - One of the best experts on this subject based on the ideXlab platform.
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transcriptome analysis of the human t lymphocyte Cell Line jurkat and human peripheral blood mononuclear Cells exposed to deoxynivalenol don new mechanistic insights
Toxicology and Applied Pharmacology, 2012Co-Authors: Madhumohan R Katika, Peter J M Hendriksen, Jia Shao, Henk Van Loveren, Ad A C M PeijnenburgAbstract:Deoxynivalenol (DON) or vomitoxin is a commonly encountered type-B trichothecene mycotoxin, produced by Fusarium species predominantly found in cereals and grains. DON is known to exert toxic effects on the gastrointestinal, reproductive and neuroendocrine systems, and particularly on the immune system. Depending on dose and exposure time, it can either stimulate or suppress immune function. The main objective of this study was to obtain a deeper insight into DON-induced effects on lymphoid Cells. For this, we exposed the human T-Lymphocyte Cell Line Jurkat and human peripheral blood mononuclear Cells (PBMCs) to various concentrations of DON for various times and examined gene expression changes by DNA microarray analysis. Jurkat Cells were exposed to 0.25 and 0.5μM DON for 3, 6 and 24h. Biological interpretation of the microarray data indicated that DON affects various processes in these Cells: It upregulates genes involved in ribosome structure and function, RNA/protein synthesis and processing, endoplasmic reticulum (ER) stress, calcium-mediated signaling, mitochondrial function, oxidative stress, the NFAT and NF-κB/TNF-α pathways, T Cell activation and apoptosis. The effects of DON on the expression of genes involved in ER stress, NFAT activation and apoptosis were confirmed by qRT-PCR. Other biochemical experiments confirmed that DON activates calcium-dependent proteins such as calcineurin and M-calpain that are known to be involved in T Cell activation and apoptosis. Induction of T Cell activation was also confirmed by demonstrating that DON activates NFATC1 and induces its translocation from the cytoplasm to the nucleus. For the gene expression profiling of PBMCs, Cells were exposed to 2 and 4μM DON for 6 and 24h. Comparison of the Jurkat microarray data with those obtained with PBMCs showed that most of the processes affected by DON in the Jurkat Cell Line were also affected in the PBMCs.
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transcriptome analysis of the human t lymphocyte Cell Line jurkat and human peripheral blood mononuclear Cells exposed to deoxynivalenol don new mechanistic insights
Toxicology and Applied Pharmacology, 2012Co-Authors: Madhumohan R Katika, Peter J M Hendriksen, Henk Van Loveren, Jia Shao, Ad A C M PeijnenburgAbstract:Deoxynivalenol (DON) or vomitoxin is a commonly encountered type-B trichothecene mycotoxin, produced by Fusarium species predominantly found in cereals and grains. DON is known to exert toxic effects on the gastrointestinal, reproductive and neuroendocrine systems, and particularly on the immune system. Depending on dose and exposure time, it can either stimulate or suppress immune function. The main objective of this study was to obtain a deeper insight into DON-induced effects on lymphoid Cells. For this, we exposed the human T-Lymphocyte Cell Line Jurkat and human peripheral blood mononuclear Cells (PBMCs) to various concentrations of DON for various times and examined gene expression changes by DNA microarray analysis. Jurkat Cells were exposed to 0.25 and 0.5 mu M DON for 3, 6 and 24 h. Biological interpretation of the microarray data indicated that DON affects various processes in these Cells: It upregulates genes involved in ribosome structure and function, RNA/protein synthesis and processing, endoplasmic reticulum (ER) stress, calcium-mediated signaling, mitochondrial function, oxidative stress, the NFAT and NF-kappa B/TNF-alpha pathways, T Cell activation and apoptosis. The effects of DON on the expression of genes involved in ER stress, NFAT activation and apoptosis were confirmed by qRT-PCR. Other biochemical experiments confirmed that DON activates calcium-dependent proteins such as calcineurin and M-calpain that are known to be involved in T Cell activation and apoptosis. Induction of T Cell activation was also confirmed by demonstrating that DON activates NFATC1 and induces its translocation from the cytoplasm to the nucleus. For the gene expression profiling of PBMCs, Cells were exposed to 2 and 4 mu M DON for 6 and 24 h. Comparison of the Jurkat microarray data with those obtained with PBMCs showed that most of the processes affected by DON in the Jurkat Cell Line were also affected in the PBMCs. (C) 2012 Elsevier Inc. All rights reserved.
