T6 Antigen

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Bernadette M. Dutia - One of the best experts on this subject based on the ideXlab platform.

  • Amino-terminal sequencing of sheep CD1 Antigens and identification of a sheep CD1D gene
    Immunogenetics, 1999
    Co-Authors: S. M. Rhind, John Hopkins, Bernadette M. Dutia
    Abstract:

     The anti-CD1 monoclonal antibodies IAH-CC14 and SBU-T6 were used to immunopurify CD1 Antigens from sheep thymocytes. The amino-terminal sequence of IAH-CC14 yielded 13 amino acids, and 29 amino acids were obtained from the SBU-T6 Antigen. The sequence of the IAH-CC14 Antigen was 100% identical to the predicted sequence of the sheep CD1B clone, SCD1B-42 . The 29 amino acid sequence of the SBU-T6 Antigen did not match identically with the derived amino acid sequence of any of the previously reported sheep CD1 genes but had closest similarity to the derived sequence of human CD1E . Degenerate polymerase chain reaction primers based on this sequence identified a group 2 sheep CD1 gene. The predicted amino acid sequence of this gene shows that it is not identical to the SBU-T6 peptide, indicating that a different, CD1D -like gene was cloned.

S. M. Rhind - One of the best experts on this subject based on the ideXlab platform.

  • Amino-terminal sequencing of sheep CD1 Antigens and identification of a sheep CD1D gene
    Immunogenetics, 1999
    Co-Authors: S. M. Rhind, John Hopkins, Bernadette M. Dutia
    Abstract:

     The anti-CD1 monoclonal antibodies IAH-CC14 and SBU-T6 were used to immunopurify CD1 Antigens from sheep thymocytes. The amino-terminal sequence of IAH-CC14 yielded 13 amino acids, and 29 amino acids were obtained from the SBU-T6 Antigen. The sequence of the IAH-CC14 Antigen was 100% identical to the predicted sequence of the sheep CD1B clone, SCD1B-42 . The 29 amino acid sequence of the SBU-T6 Antigen did not match identically with the derived amino acid sequence of any of the previously reported sheep CD1 genes but had closest similarity to the derived sequence of human CD1E . Degenerate polymerase chain reaction primers based on this sequence identified a group 2 sheep CD1 gene. The predicted amino acid sequence of this gene shows that it is not identical to the SBU-T6 peptide, indicating that a different, CD1D -like gene was cloned.

Wolfgang Göttinger - One of the best experts on this subject based on the ideXlab platform.

  • T6-positive Langerhans cells in diseased corneas.
    Investigative Ophthalmology & Visual Science, 1991
    Co-Authors: Wolfgang Philipp, Wolfgang Göttinger
    Abstract:

    Langerhans cells (LC) in normal human corneas (with the exception of newborns) lack thymocyte Antigen T6, a highly specific marker for noncorneal LC. Because corneal LC could not be induced to express T6 Antigen when cultured with various cytokines including interleukin-1 (shown to modulate T6 expression on gingival LC), some authors assume that corneal LC may represent a distinct LC subpopulation that is innately inactive. In this study, 62 corneas from patients with various corneal diseases were investigated for the presence of T6 and histocompatibility Antigen HLA-DR on LC in the central and pericentral epithelium. Both T6- and HLA-DR-positive LC at a high density similar to that observed in normal epidermis could be detected in the epithelium of five corneas with epidermalization after alkali burns. Furthermore T6- and HLA-DR-positive LC at smaller densities also were detected in corneas from patients with chronic herpetic stromal keratitis, zoster keratitis, chronic allograft rejection, and bacterial corneal ulcers. Although the functional significance of T6 expression on corneal LC remains to be determined, the induction of T6 Antigen on corneal LC may represent an important event for the Antigen-presenting function of these cells in various corneal diseases including corneal allograft rejection.

E Panconesi - One of the best experts on this subject based on the ideXlab platform.

  • expression of T6 Antigen on keratinocytes in alopecia areata
    International Journal of Dermatology, 1992
    Co-Authors: Torello Lotti, Birgit R Knoepfel, Marcello Zangheri, E Panconesi
    Abstract:

    The expression of T6 Antigen within hair follicles in alopecia areata was studied using the APAAP technique (alkaline phosphatase monoclonal anti-alkaline phosphatase method). Scalp biopsies were taken from 15 subjects with alopecia areata, nine in an active stage and 6 in a stationary stage of the disease. Six-micrometer-thick frozen sections were stained with OKT6 antiserum. OKT6 are monoclonal antibodies raised against human thymocytes; they cross-react with epidermal Langerhans ceils and are a highly specific marker. Nine of the specimens displayed T6 staining on keratinocytes in the bulb matrix, and all nine were from the subjects presenting the active stage of disease. The specimens from the other six biopsies, from subjects in a stationary stage, did not show T6 staining of bulbar keratinocytes. Moreover, in four of the active-stage cases we found T6 staining also on epidermal keratinocytes.

John Hopkins - One of the best experts on this subject based on the ideXlab platform.

  • Amino-terminal sequencing of sheep CD1 Antigens and identification of a sheep CD1D gene
    Immunogenetics, 1999
    Co-Authors: S. M. Rhind, John Hopkins, Bernadette M. Dutia
    Abstract:

     The anti-CD1 monoclonal antibodies IAH-CC14 and SBU-T6 were used to immunopurify CD1 Antigens from sheep thymocytes. The amino-terminal sequence of IAH-CC14 yielded 13 amino acids, and 29 amino acids were obtained from the SBU-T6 Antigen. The sequence of the IAH-CC14 Antigen was 100% identical to the predicted sequence of the sheep CD1B clone, SCD1B-42 . The 29 amino acid sequence of the SBU-T6 Antigen did not match identically with the derived amino acid sequence of any of the previously reported sheep CD1 genes but had closest similarity to the derived sequence of human CD1E . Degenerate polymerase chain reaction primers based on this sequence identified a group 2 sheep CD1 gene. The predicted amino acid sequence of this gene shows that it is not identical to the SBU-T6 peptide, indicating that a different, CD1D -like gene was cloned.