The Experts below are selected from a list of 294 Experts worldwide ranked by ideXlab platform
Yeong Shik Kim - One of the best experts on this subject based on the ideXlab platform.
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Purification and characterization of anticoagulant protein from the Tabanus, Tabanus bivittatus.
Archives of pharmacal research, 2006Co-Authors: Mi Young Ahn, Bum Soo Hahn, Pyeong Jae Lee, Yeong Shik KimAbstract:Tabanus anticoagulant protein (TAP) was isolated from the whole body of the Tabanus, Tabanus bivittatus, using three purification steps (ammonium sulfate fractionation, gel filtration on Bio-Gel P-60, and ion exchange chromatography on DEAE Sephadex gel). The purified TAP, with a molecular weight of 65 kDa, was assessed to be homogeneous by SDS-polyacrylamide gel electrophoresis, and an isoelectric point of 7.9 was determined by isoelectric focusing. The internal amino acid sequence of the purified protein was composed of Ser-Leu-Asn-Asn-Gln-Phe-Ala-Ser-Phe-Ile-Asp-Lys-Val-Arg. The protein was activated by Cu2+ and Zn2+, and the optimal conditions were found to be at pH 3-6 and 40-70 degrees C. Standard coagulation screen assays were used to determine thrombin time and activated partial thromboplastin time. Chromogenic substrate assays were performed for thrombin and factor Xa activity. TAP considerably prolonged human plasma clotting time, especially activated partial thromboplastin time in a dose-dependent manner; it showed potent and specific antithrombin activity in the chromogenic substrate assay. Specific anti-factor Xa activity in TAP was not detected. Overall, this result suggested that TAP has significant anticoagulant activity on blood coagulation system.
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Cytotoxicity and L-amino acid oxidase activity of crude insect drugs
Archives of Pharmacal Research, 2000Co-Authors: Mi Young Ahn, Kang Sun Ryu, Yong Woo Lee, Yeong Shik KimAbstract:The cytotoxicity of crude insect drugs was measured using HeLa cells originating from human cervix and uterine cancer, using the dye uptake assay in order to find potential anticancer agents. Three kinds of extracts (buffer, methanol and ethylacetate) were prepared from 26 insects and used as raw materials for the activity assay. Among these, the buffer extracts from Tabanus, Mylabris and Huechys showed a potent anticancer activity, and those from Catharsius, Red ant, Scorpion, Tabanus and Vespae Nidus showed a strong L-amino acid oxidase (AAO) activity as well as cytotoxicity. In contrast, buffer extracts from Cryllotalpa orientalis and Apriona germari larvae showed greater/more rapid Hela cell growth than that of other insects.
Xu Rong - One of the best experts on this subject based on the ideXlab platform.
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TWO NEW SPECIES OF Tabanus BIANNULARIS GROUP FROM CHINA(DIPTERA:TABANIDAE)
2008Co-Authors: Xu RongAbstract:Two new species of Tabanus biannularis group belonging to Genus Tabanus fron China were described in this paper.The holotypes collected from following:Tabanus parasexcinctus from Tianmu Mountains,Zhejiang Province;Tabanus tianyui from Tengchong County,Yunnan Province.
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two new species of Tabanus aurisetosus group from china diptera tabanidae
Acta Parasitologica Et Medica Entomologica Sinica, 2008Co-Authors: Xu RongAbstract:Two new species of Tabanus aurisetosus group belonging to genus Tabanus were described from China in this paper.The holotypes were collected from following: Tabanus baohaii from Meihuashan Mountain,Fujian(25.2°N,116.7°E,);Tabanus paraflavimarginatus from Jianfenglin Mountain,Hainan(18.7°N,108.9°E).Total 8 species of the group have been known from China and arranged in the key.T.baohaii is similar to T.cylindrocallus Wang,but with no spots of white hairs on the dorsum of abdomen in T.cylindrocallus.T.paraflavimarginatus is near T.flavimarginatus Schuurmans Stekhoven,legs brown,wing R_4 with no spur in T.paraflavimarginatus,but femora brownish blach,tibiae white,tarsi black,R_4 with a spur in T.flavimarginatus.
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TWO NEW SPECIES OF Tabanus OLIVIVENTRIS GROUP FROM CHINA(DIPTERA:TABANIDAE)
2007Co-Authors: Xu RongAbstract:Two new species of Tabanus oliviventris group belonging to genus Tabanus were described from China in this paper.The holotypes were collected from following:Tabanus jinhuai from Wuzhishan(27.3°N,118.2°E,720-950 m),Hainan;Tabanus tieshengi from Mutuo County(29°N,95°E,900 m),Tibet.Total 17 species of the group have been known from China and arranged in the key.Tabanus jinhuai is similar to T.huangshanensis Xu et Wu,terga of abdomen black with white bands at hind margins in jinhuai,but no white bands in huangshanensis.Tabanus tieshengi is near T.parviformus Wang,fore femur brown in T.tieshengi,but black in T.parviformus.
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FOUR NEW SPECIES OF TABANIDAE FROM YUNNAN,CHINA(DIPTERA)
2005Co-Authors: Xu RongAbstract:Three new species of genus Tabanus,one new species of genus Haematopota from Yunnan,China were described.The types are deposited in the Institute of Microbiology and Epidemiology,AMMS,Beijing.The holotypes collected from following:Tabanus xuezhongi from Lushui County(26°N,98.5°E,1 900m);Tabanus zhongpingi from Cangyuan County(23.2°N,99.2°E,1 500m);Tabanus paradiversifrons from Lushui County(25.8°N,98.8°E,1 000m);Haematopota mengdingensis from Mengding,Gengma County(23.5°N,99°E,500m).
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THREE NEW SPECIES OF TABANIDAE FROM YUNNAN,CHINA(DIPTERA)
2005Co-Authors: Xu RongAbstract:Two new species of genus Tabanus, one new species of genus Haematopota from Yunnan,China were described.The types are deposited in the Institute of Microbiology and Epidemiology,AMMS,Beijing.The holotypes collected from following:Tabanus daohaoi from Mengla County(21.2°N,101.8°E,800m);Tabanus liangqingi from mengla County(21.9°N,101.2°E,500m);Haematopota burtoni from Mengla County(21.2°N,101.8°E,800m).Tabanus laotianus(Bigot,1890),T.monilifer(Bigot,1892),T.rubicundus Macquart,1846 and T.paviei Burton,1978 are as new records for China.
Mi Young Ahn - One of the best experts on this subject based on the ideXlab platform.
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Purification and characterization of anticoagulant protein from the Tabanus, Tabanus bivittatus.
Archives of pharmacal research, 2006Co-Authors: Mi Young Ahn, Bum Soo Hahn, Pyeong Jae Lee, Yeong Shik KimAbstract:Tabanus anticoagulant protein (TAP) was isolated from the whole body of the Tabanus, Tabanus bivittatus, using three purification steps (ammonium sulfate fractionation, gel filtration on Bio-Gel P-60, and ion exchange chromatography on DEAE Sephadex gel). The purified TAP, with a molecular weight of 65 kDa, was assessed to be homogeneous by SDS-polyacrylamide gel electrophoresis, and an isoelectric point of 7.9 was determined by isoelectric focusing. The internal amino acid sequence of the purified protein was composed of Ser-Leu-Asn-Asn-Gln-Phe-Ala-Ser-Phe-Ile-Asp-Lys-Val-Arg. The protein was activated by Cu2+ and Zn2+, and the optimal conditions were found to be at pH 3-6 and 40-70 degrees C. Standard coagulation screen assays were used to determine thrombin time and activated partial thromboplastin time. Chromogenic substrate assays were performed for thrombin and factor Xa activity. TAP considerably prolonged human plasma clotting time, especially activated partial thromboplastin time in a dose-dependent manner; it showed potent and specific antithrombin activity in the chromogenic substrate assay. Specific anti-factor Xa activity in TAP was not detected. Overall, this result suggested that TAP has significant anticoagulant activity on blood coagulation system.
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Cytotoxicity and L-amino acid oxidase activity of crude insect drugs
Archives of Pharmacal Research, 2000Co-Authors: Mi Young Ahn, Kang Sun Ryu, Yong Woo Lee, Yeong Shik KimAbstract:The cytotoxicity of crude insect drugs was measured using HeLa cells originating from human cervix and uterine cancer, using the dye uptake assay in order to find potential anticancer agents. Three kinds of extracts (buffer, methanol and ethylacetate) were prepared from 26 insects and used as raw materials for the activity assay. Among these, the buffer extracts from Tabanus, Mylabris and Huechys showed a potent anticancer activity, and those from Catharsius, Red ant, Scorpion, Tabanus and Vespae Nidus showed a strong L-amino acid oxidase (AAO) activity as well as cytotoxicity. In contrast, buffer extracts from Cryllotalpa orientalis and Apriona germari larvae showed greater/more rapid Hela cell growth than that of other insects.
Dhriti Banerjee - One of the best experts on this subject based on the ideXlab platform.
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A new species of the genus Tabanus linnaeus (Diptera: Tabanidae) from the West Bengal
Indian Journal of Entomology, 2017Co-Authors: Aniruddha Maity, Atanu Naskar, Surajit Hazra, Jayita Sengupta, P. Parui, Dhriti BanerjeeAbstract:A new species, Tabanus pseudoannularis n. sp. of the horse fly genus Tabanus, from West Bengal, India is described and illustrated. Its morphological differences and association with T. biannularis is also discussed.
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Tabanid Fauna (Diptera: Tabanidae) along The Lower Most Gangetic Plains of India: A Taxonomic Approach
Indian Journal of Entomology, 2017Co-Authors: Aniruddha Maity, Atanu Naskar, Surajit Hazra, Jayita Sengupta, Dhriti Banerjee, P. ParuiAbstract:True flies of family Tabanidae (Brachycera, Tabanomorpha) refers to group of sturdy, handsome, large headed flies devoid of large bristles and with colourful eyes. The present study reports 21 species under 6 genera, 4 tribes and 2 subfamilies from the Gangetic plains of West Bengal. These are also keyed, diagnosed and their distribution pattern discussed. Among these Chrysops designatus Ricardo (1911), Tabanus dorsiger Wiedemann (1821), Tabanus (Tabanus) optatus Walker (1856), Tabanus (Tabanus) tenens Walker (1850), and Haematopota tessellata Ricardo, (1906) are new records from the lowermost Gangetic plains and also from West Bengal. Continuous distribution pattern was observed with these flies of veterinary importance and haematophagous nature.
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New distributional records of Tabanidae (Insecta: Diptera) from different geo-climatic regions of West Bengal, India
Journal of entomology and zoology studies, 2016Co-Authors: Aniruddha Maity, Atanu Naskar, Surajit Hazra, Jayita Sengupta, P. Parui, Sumit Homechaudhuri, Dhriti BanerjeeAbstract:Eight species namely Chrysops designatus Ricardo, 1911; Hybomitra subcallosa (Ricardo, 1911); Tabanus dorsiger Wiedemann, 1821; Tabanus (Tabanus) biannularis Philip, 1960; Tabanus (Tabanus) optatus Walker, 1856; Haematopota inconspicua Ricardo, 1911; Haematopota sikkimensis Stone & Philip, 1974 and Haematopota tessellata Ricardo, 1906; belonging to four genera viz., Chrysops Meigen, 1803; Hybomitra Enderlein, 1922; Tabanus Linnaeus, 1758 and Haematopota Meigen, 1803; of subfamilies Chrysopsinae and Tabaninae, are recorded for the first time from the state of West Bengal, are listed, keyed, and discussed with distribution pattern wherever deemed necessary, along with morphology and methodology in aid of understanding the flies of family Tabanidae.
Patricia A. Nuttall - One of the best experts on this subject based on the ideXlab platform.
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Vasodilatory activity in horsefly and deerfly salivary glands.
Medical and Veterinary Entomology, 2003Co-Authors: P. Rajska, Ladislav Roller, L. Vidlička, Milan Labuda, Fedor Čiampor, Peter Takac, Mária Kazimírová, Olga Pechanova, Patricia A. NuttallAbstract:. Salivary gland extract (SGE) of four horsefly species (Hybomitra bimaculata Macquart, Hybomitra ciureai Seguy, Tabanus bromius L., Tabanus glaucopis Meigen) and one deerfly species (Chrysops relictus Meigen) (Diptera: Tabanidae) were shown to contain vasodilatory activity. Aliquots equivalent to 1, 5 and 10 pairs of salivary glands (SG) relaxed rat femoral artery (with intact endothelium) pre-constricted with phenylephrine. Vasodilatory activity was dose-dependent. SGE of one horsefly species (Haematopota pluvialis L.) did not induce relaxation. The kinetics of vasodilation induced by SGE of four horsefly species differed from the deerfly. These results indicate that tabanid species may produce more than one type of vasodilator to aid blood feeding.
