The Experts below are selected from a list of 18 Experts worldwide ranked by ideXlab platform
Kamil Kuca - One of the best experts on this subject based on the ideXlab platform.
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Bisquaternary oximes as reactivators of Tabun-inhibited human brain cholinesterases: an in vitro study.
Basic & clinical pharmacology & toxicology, 2007Co-Authors: Kamil Kuca, Daniel Jun, Jiri Cabal, Lucie MusilovaAbstract:Intoxications caused by Tabun Nerve Agent are generally very hard to treat by convential acetylcholinesterase (AChE) reactivators. Due to this, new AChE reactivators are still developed. In this study, we have tested three new promising bisquaternary AChE reactivators: K027, K033 and K048. These reactivators were previously tested on rat brain homogenate. To mimic reality, we studied the potency of these new oximes to reactivate Tabun-inhibited human brain cholinesterases. As is evident from the results, reactivator K048 (reactivation 40%) surpassed all reactivators tested in this study [including the most promising ones, namely trimedoxime (37%) and obidoxime (33%)]. Moreover, if compared to our previous results from rat brain studies, species differences were demonstrated.
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In Vitro Evaluation of Acetylcholinesterase Reactivators as Potential Antidotes Against Tabun Nerve Agent Poisonings
Drug and chemical toxicology, 2006Co-Authors: Kamil Kuca, Daniel Jun, Jiri Cabal, Martina HrabinovaAbstract:Searching for new potent acetylcholinesterase (AChE; E.C. 3.1.1.7) reactivators (oximes) is a very time-consuming process. At our department, we are able to synthesize more than 50 new AChE reactivators per year. Owing to this fact, we have to select promising reactivators using our in vitro method (potentiometric titration, pH 8 and temperature 25 degrees C; source of cholinesterases, rat brain homogenate; time of inhibition by Nerve Agents, 30 min; time of reactivation, 10 min) prior to in vivo experiments. For this purpose, we are using two-phase in vitro evaluation of reactivator potency. In the first phase, reactivation potency of all newly synthesized AChE reactivators is tested at two concentrations: 10(-3) M and 10(-5) M. Afterwards, all reactivators achieving reactivation potency over 15% (especially at the concentration 10(-5) M) are tested. The second phase consists of the measurement of the relationship between concentration of the oxime and its reactivation ability. In most cases, the reactivation bell-shaped curve is obtained. The most potent AChE reactivators are selected and provided for further experiments during our development process.
Jiri Cabal - One of the best experts on this subject based on the ideXlab platform.
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Bisquaternary oximes as reactivators of Tabun-inhibited human brain cholinesterases: an in vitro study.
Basic & clinical pharmacology & toxicology, 2007Co-Authors: Kamil Kuca, Daniel Jun, Jiri Cabal, Lucie MusilovaAbstract:Intoxications caused by Tabun Nerve Agent are generally very hard to treat by convential acetylcholinesterase (AChE) reactivators. Due to this, new AChE reactivators are still developed. In this study, we have tested three new promising bisquaternary AChE reactivators: K027, K033 and K048. These reactivators were previously tested on rat brain homogenate. To mimic reality, we studied the potency of these new oximes to reactivate Tabun-inhibited human brain cholinesterases. As is evident from the results, reactivator K048 (reactivation 40%) surpassed all reactivators tested in this study [including the most promising ones, namely trimedoxime (37%) and obidoxime (33%)]. Moreover, if compared to our previous results from rat brain studies, species differences were demonstrated.
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In Vitro Evaluation of Acetylcholinesterase Reactivators as Potential Antidotes Against Tabun Nerve Agent Poisonings
Drug and chemical toxicology, 2006Co-Authors: Kamil Kuca, Daniel Jun, Jiri Cabal, Martina HrabinovaAbstract:Searching for new potent acetylcholinesterase (AChE; E.C. 3.1.1.7) reactivators (oximes) is a very time-consuming process. At our department, we are able to synthesize more than 50 new AChE reactivators per year. Owing to this fact, we have to select promising reactivators using our in vitro method (potentiometric titration, pH 8 and temperature 25 degrees C; source of cholinesterases, rat brain homogenate; time of inhibition by Nerve Agents, 30 min; time of reactivation, 10 min) prior to in vivo experiments. For this purpose, we are using two-phase in vitro evaluation of reactivator potency. In the first phase, reactivation potency of all newly synthesized AChE reactivators is tested at two concentrations: 10(-3) M and 10(-5) M. Afterwards, all reactivators achieving reactivation potency over 15% (especially at the concentration 10(-5) M) are tested. The second phase consists of the measurement of the relationship between concentration of the oxime and its reactivation ability. In most cases, the reactivation bell-shaped curve is obtained. The most potent AChE reactivators are selected and provided for further experiments during our development process.
Daniel Jun - One of the best experts on this subject based on the ideXlab platform.
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Bisquaternary oximes as reactivators of Tabun-inhibited human brain cholinesterases: an in vitro study.
Basic & clinical pharmacology & toxicology, 2007Co-Authors: Kamil Kuca, Daniel Jun, Jiri Cabal, Lucie MusilovaAbstract:Intoxications caused by Tabun Nerve Agent are generally very hard to treat by convential acetylcholinesterase (AChE) reactivators. Due to this, new AChE reactivators are still developed. In this study, we have tested three new promising bisquaternary AChE reactivators: K027, K033 and K048. These reactivators were previously tested on rat brain homogenate. To mimic reality, we studied the potency of these new oximes to reactivate Tabun-inhibited human brain cholinesterases. As is evident from the results, reactivator K048 (reactivation 40%) surpassed all reactivators tested in this study [including the most promising ones, namely trimedoxime (37%) and obidoxime (33%)]. Moreover, if compared to our previous results from rat brain studies, species differences were demonstrated.
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In Vitro Evaluation of Acetylcholinesterase Reactivators as Potential Antidotes Against Tabun Nerve Agent Poisonings
Drug and chemical toxicology, 2006Co-Authors: Kamil Kuca, Daniel Jun, Jiri Cabal, Martina HrabinovaAbstract:Searching for new potent acetylcholinesterase (AChE; E.C. 3.1.1.7) reactivators (oximes) is a very time-consuming process. At our department, we are able to synthesize more than 50 new AChE reactivators per year. Owing to this fact, we have to select promising reactivators using our in vitro method (potentiometric titration, pH 8 and temperature 25 degrees C; source of cholinesterases, rat brain homogenate; time of inhibition by Nerve Agents, 30 min; time of reactivation, 10 min) prior to in vivo experiments. For this purpose, we are using two-phase in vitro evaluation of reactivator potency. In the first phase, reactivation potency of all newly synthesized AChE reactivators is tested at two concentrations: 10(-3) M and 10(-5) M. Afterwards, all reactivators achieving reactivation potency over 15% (especially at the concentration 10(-5) M) are tested. The second phase consists of the measurement of the relationship between concentration of the oxime and its reactivation ability. In most cases, the reactivation bell-shaped curve is obtained. The most potent AChE reactivators are selected and provided for further experiments during our development process.
Lucie Musilova - One of the best experts on this subject based on the ideXlab platform.
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Bisquaternary oximes as reactivators of Tabun-inhibited human brain cholinesterases: an in vitro study.
Basic & clinical pharmacology & toxicology, 2007Co-Authors: Kamil Kuca, Daniel Jun, Jiri Cabal, Lucie MusilovaAbstract:Intoxications caused by Tabun Nerve Agent are generally very hard to treat by convential acetylcholinesterase (AChE) reactivators. Due to this, new AChE reactivators are still developed. In this study, we have tested three new promising bisquaternary AChE reactivators: K027, K033 and K048. These reactivators were previously tested on rat brain homogenate. To mimic reality, we studied the potency of these new oximes to reactivate Tabun-inhibited human brain cholinesterases. As is evident from the results, reactivator K048 (reactivation 40%) surpassed all reactivators tested in this study [including the most promising ones, namely trimedoxime (37%) and obidoxime (33%)]. Moreover, if compared to our previous results from rat brain studies, species differences were demonstrated.
Ladan Edjlali - One of the best experts on this subject based on the ideXlab platform.
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Possibility of sensing, adsorbing, and destructing the Tabun-2D-skeletal (Tabun Nerve Agent) by C20 fullerene and its boron and nitrogen doped derivatives
Synthetic Metals, 2016Co-Authors: Seyyed Amir Siadati, Esmail Vessally, Akram Hosseinian, Ladan EdjlaliAbstract:Abstract Tabun Nerve Agent has taken hundreds thousands of lives during the last century. This chemical warfare was widely used against peoples in many areas of the world, and brought painful death for the victims. Therefore, this hazardous gas has been known as one of the most important chemical Agents for the crime of mass killing. Following efforts of many researchers throughout the worlds to control the unwanted effects of mass killing warfares, in this project, we have made efforts of find a way to inactive this dangerous species by adsorption of this molecule by C20 fullerene and its boron and nitrogen doped derivatives. Moreover, by using the density of state DOS plots, the changes of electrical conductivity of C20 fullerene both in presence and in absence of Tabun gas were investigated to know is C20 fullerene able to be used as a sensor for detecting the existence of this species?
