TAC3

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Xiaoxia Han - One of the best experts on this subject based on the ideXlab platform.

  • two dimensional tantalum carbide mxenes composite nanosheets for multiple imaging guided photothermal tumor ablation
    ACS Nano, 2017
    Co-Authors: Chen Dai, Han Lin, Zhuang Liu, Yu Chen, Xiangxiang Jing, Lihua Xiang, Dayang Yang, Xiaoxia Han
    Abstract:

    MXenes, an emerging family of graphene-analogues two-dimensional (2D) materials, have attracted continuous and tremendous attention in many application fields because of their intrinsic physiochemical properties and high performance in versatile applications. In this work, we report on the construction of tantalum carbide (Ta4C3) MXene-based composite nanosheets for multiple imaging-guided photothermal tumor ablation, which has been achieved by rational choice of the composition of MXenes and their surface functionalization. A redox reaction was activated on the surface of tantalum carbide (Ta4C3) MXene for in situ growth of manganese oxide nanoparticles (MnOx/Ta4C3) based on the reducing surface of the nanosheets. The tantalum components of MnOx/Ta4C3 acted as the high-performance contrast agents for contrast-enhanced computed tomography, and the integrated MnOx component functionalized as the tumor microenvironment-responsive contrast agents for T1-weighted magnetic resonance imaging. The photothermal-conversion performance of MnOx/Ta4C3 composite nanosheets not only has achieved contrast-enhanced photoacoustic imaging, but also realized the significant tumor-growth suppression by photothermal hyperthermia. This work broadens the biomedical applications of MXenes, not only by the fabrication of family members of biocompatible MXenes, but also by the development of functionalization strategies of MXenes for cancer-theranostic applications.

  • Two-Dimensional Tantalum Carbide (MXenes) Composite Nanosheets for Multiple Imaging-Guided Photothermal Tumor Ablation
    2017
    Co-Authors: Chen Dai, Han Lin, Zhuang Liu, Yu Chen, Xiangxiang Jing, Lihua Xiang, Dayang Yang, Xiaoxia Han
    Abstract:

    MXenes, an emerging family of graphene-analogues two-dimensional (2D) materials, have attracted continuous and tremendous attention in many application fields because of their intrinsic physiochemical properties and high performance in versatile applications. In this work, we report on the construction of tantalum carbide (Ta4C3) MXene-based composite nanosheets for multiple imaging-guided photothermal tumor ablation, which has been achieved by rational choice of the composition of MXenes and their surface functionalization. A redox reaction was activated on the surface of tantalum carbide (Ta4C3) MXene for in situ growth of manganese oxide nanoparticles (MnOx/Ta4C3) based on the reducing surface of the nanosheets. The tantalum components of MnOx/Ta4C3 acted as the high-performance contrast agents for contrast-enhanced computed tomography, and the integrated MnOx component functionalized as the tumor microenvironment-responsive contrast agents for T1-weighted magnetic resonance imaging. The photothermal-conversion performance of MnOx/Ta4C3 composite nanosheets not only has achieved contrast-enhanced photoacoustic imaging, but also realized the significant tumor-growth suppression by photothermal hyperthermia. This work broadens the biomedical applications of MXenes, not only by the fabrication of family members of biocompatible MXenes, but also by the development of functionalization strategies of MXenes for cancer-theranostic applications

Chen Dai - One of the best experts on this subject based on the ideXlab platform.

  • two dimensional tantalum carbide mxenes composite nanosheets for multiple imaging guided photothermal tumor ablation
    ACS Nano, 2017
    Co-Authors: Chen Dai, Han Lin, Zhuang Liu, Yu Chen, Xiangxiang Jing, Lihua Xiang, Dayang Yang, Xiaoxia Han
    Abstract:

    MXenes, an emerging family of graphene-analogues two-dimensional (2D) materials, have attracted continuous and tremendous attention in many application fields because of their intrinsic physiochemical properties and high performance in versatile applications. In this work, we report on the construction of tantalum carbide (Ta4C3) MXene-based composite nanosheets for multiple imaging-guided photothermal tumor ablation, which has been achieved by rational choice of the composition of MXenes and their surface functionalization. A redox reaction was activated on the surface of tantalum carbide (Ta4C3) MXene for in situ growth of manganese oxide nanoparticles (MnOx/Ta4C3) based on the reducing surface of the nanosheets. The tantalum components of MnOx/Ta4C3 acted as the high-performance contrast agents for contrast-enhanced computed tomography, and the integrated MnOx component functionalized as the tumor microenvironment-responsive contrast agents for T1-weighted magnetic resonance imaging. The photothermal-conversion performance of MnOx/Ta4C3 composite nanosheets not only has achieved contrast-enhanced photoacoustic imaging, but also realized the significant tumor-growth suppression by photothermal hyperthermia. This work broadens the biomedical applications of MXenes, not only by the fabrication of family members of biocompatible MXenes, but also by the development of functionalization strategies of MXenes for cancer-theranostic applications.

  • Two-Dimensional Tantalum Carbide (MXenes) Composite Nanosheets for Multiple Imaging-Guided Photothermal Tumor Ablation
    2017
    Co-Authors: Chen Dai, Han Lin, Zhuang Liu, Yu Chen, Xiangxiang Jing, Lihua Xiang, Dayang Yang, Xiaoxia Han
    Abstract:

    MXenes, an emerging family of graphene-analogues two-dimensional (2D) materials, have attracted continuous and tremendous attention in many application fields because of their intrinsic physiochemical properties and high performance in versatile applications. In this work, we report on the construction of tantalum carbide (Ta4C3) MXene-based composite nanosheets for multiple imaging-guided photothermal tumor ablation, which has been achieved by rational choice of the composition of MXenes and their surface functionalization. A redox reaction was activated on the surface of tantalum carbide (Ta4C3) MXene for in situ growth of manganese oxide nanoparticles (MnOx/Ta4C3) based on the reducing surface of the nanosheets. The tantalum components of MnOx/Ta4C3 acted as the high-performance contrast agents for contrast-enhanced computed tomography, and the integrated MnOx component functionalized as the tumor microenvironment-responsive contrast agents for T1-weighted magnetic resonance imaging. The photothermal-conversion performance of MnOx/Ta4C3 composite nanosheets not only has achieved contrast-enhanced photoacoustic imaging, but also realized the significant tumor-growth suppression by photothermal hyperthermia. This work broadens the biomedical applications of MXenes, not only by the fabrication of family members of biocompatible MXenes, but also by the development of functionalization strategies of MXenes for cancer-theranostic applications

Taneli Raivio - One of the best experts on this subject based on the ideXlab platform.

  • GnRH receptor gene mutations in adolescents and young adults presenting with signs of partial gonadotropin deficiency
    PloS one, 2017
    Co-Authors: Johanna Hietamäki, Matti Hero, Elina Holopainen, Johanna Känsäkoski, Kirsi Vaaralahti, Anna-pauliina Iivonen, Päivi J. Miettinen, Taneli Raivio
    Abstract:

    Biallelic, partial loss-of-function mutations in GNRHR cause a wide spectrum of reproductive phenotypes from constitutional delay of growth and puberty to complete congenital hypogonadotropic hypogonadism. We studied the frequency of GNRHR, FGFR1, TAC3, and TACR3 mutations in nine adolescent and young adult females with clinical cues consistent with partial gonadotropin deficiency (stalled puberty, unexplained secondary amenorrhea), and describe phenotypic features and molecular genetic findings of monozygotic twin brothers with stalled puberty. Two girls out of nine (22%, 95%CI 6-55%) carried biallelic mutations in GNRHR. The girl with compound heterozygous c.317A>G p.(Gln106Arg) and c.924_926delCTT p.(Phe309del) GNRHR mutations displayed incomplete puberty and clinical signs of hypoestrogenism. The patient carrying a homozygous c.785G>A p.(Arg262Gln) mutation presented with signs of hypoestrogenism and unexplained secondary amenorrhea. None of the patients exhibited mutations in FGFR1, TAC3, or TACR3. The twin brothers, compound heterozygous for GNRHR mutations c.317A>G p.(Gln106Arg) and c.785G>A p.(Arg262Gln), presented with stalled puberty and were discordant for weight, and the heavier of them had lower testosterone levels. These results suggest that genetic testing of the GNRHR gene should be offered to adolescent females with low-normal gonadotropins and unexplained stalled puberty or menstrual dysfunction. In male patients with partial gonadotropin deficiency, excess adipose tissue may suppress hypothalamic-pituitary-gonadal axis.

  • isolated cryptorchidism no evidence for involvement of genes underlying isolated hypogonadotropic hypogonadism
    Molecular and Cellular Endocrinology, 2011
    Co-Authors: Eevamaria Laitinen, Kirsi Vaaralahti, Helena E Virtanen, Heidi Oehlandt, Rosanna Koivu, Jorma Toppari, Taneli Raivio
    Abstract:

    Abstract Mutations in FGFR1 , GNRHR , PROK2 , PROKR2 , TAC3 , or TACR3 underlie isolated hypogonadotropic hypogonadism (IHH) with clinically variable phenotypes, and, by causing incomplete intrauterine activation of the hypothalamic–pituitary–gonadal axis, may lead to cryptorchidism. To investigate the role of defects in these genes in the etiology of isolated cryptorchidism, we screened coding exons and exon–intron boundaries of these genes in 54 boys or men from 46 families with a history of cryptorchidism. Control subjects (200) included 120 males. None of the patients carried mutation(s) in FGFR1 , PROK2 , PROKR2 , TAC3 or TACR3 . Two of the 46 index subjects with unilateral cryptorchidism were heterozygous carriers of a single GNRHR mutation (Q106R or R262Q), also present in male controls with a similar frequency (3/120; p  = 0.62). No homozygous or compound heterozygous GNRHR mutations were found. In conclusion, cryptorchidism is not commonly caused by defects in genes involved in IHH.

Haoran Lin - One of the best experts on this subject based on the ideXlab platform.

  • knockout of TAC3 genes in zebrafish shows no impairment of reproduction
    General and Comparative Endocrinology, 2021
    Co-Authors: Tingting Zhao, Yun Liu, Haoran Lin, Yong Zhang
    Abstract:

    Neurokinin B (NKB) plays a pivotal role in the regulation of reproduction in vertebrates. However, whether this neuropeptide is dispensable for reproduction in teleosts remains unknown. In order to reveal its authentic functions in fish, in this study, two tachykinin 3 (TAC3) genes encoding Nkbs were functional mutated in zebrafish using the Transcription Activator-like Effector Nucleases (TALEN) technology. We established TAC3a-/-, TAC3b-/- and TAC3a-/-;TAC3b-/- mutant lines, and investigated their reproductive performance and ontogeny. According to our study, spermatogenesis and folliculogenesis were not impaired in TAC3a-/-, TAC3b-/- and TAC3a-/-;TAC3b-/- mutant lines, but changes in the expression of genes related to reproductive axis were observed after loss of TAC3, suggesting that possible compensatory response was activated to maintained the reproductive function in zebrafish. In summary, our results indicate that mutation of TAC3 genes do not disrupt the reproduction in zebrafish unlike in mammals, revealing the plasticity of reproductive neuroendocrine system in the brain of zebrafish.

  • neurokinin b signaling in hermaphroditic species a study of the orange spotted grouper epinephelus coioides
    General and Comparative Endocrinology, 2018
    Co-Authors: Huapu Chen, Yong Zhang, Ling Xiao, Yali Liu, Haoran Lin
    Abstract:

    Neurokinin B (NKB) plays important roles in the mammalian reproductive axis by modulating the release of gonadotropin-releasing hormone (GnRH) and gonadotropins. In the present study, the TAC3 cDNA was cloned from a hermaphroditic species, the orange-spotted grouper. Sequence analysis showed that the grouper TAC3 precursor encoded two tachykinin peptides, NKB and NKB-related peptide (NKBRP). Expression analysis in different tissues revealed that TAC3 mRNA was highly expressed in the brain of the orange-spotted grouper. In situ hybridization further revealed that it was localized in some hypothalamic nuclei associated with reproductive regulation. During ovarian development, an increase of TAC3 expression in the hypothalamus was observed at vitellogenesis stage. Intraperitoneal administration of NKB could increase the gnrh1 and lhβ mRNA levels, and enhance the serum estrogen levels, but did not significantly influence lhβ expression in cultured pituitary cells, indicating that NKB does not directly exert its actions on the pituitary gland. However, it was found that NKBRP had no effect on the expression of two gnrhs and two gths in vivo and in vitro. Effects of sex steroids on TAC3 expression were further investigated. During the 17-methyltestosterone-induced sex change in the orange-spotted grouper, hypothalamic TAC3 expression showed no significant change. Interestingly, ovariectomy greatly stimulated TAC3 expression, while the 17β-estradiol treatment reversed this effect. In general, our data highly indicated that NKB signaling could activate the reproductive axis in the orange-spotted grouper. Our study is the first description of the NKB signaling in the hermaphroditic species.

  • goldfish neurokinin b cloning tissue distribution and potential role in regulating reproduction
    General and Comparative Endocrinology, 2015
    Co-Authors: Wenyi Zhou, Yun Liu, Xiaochun Liu, Yong Zhang, Haoran Lin, Chun Peng
    Abstract:

    Neurokinin B (NKB) is a member of the tackykinin (TAC) family known to play a critical role in the neuroendocrine regulation of reproduction in mammals. However, its biological functions in teleosts are less clear. The aim of this study was to determine the role of NKB in fish reproduction using goldfish as a model. Two transcripts, TAC3a and TAC3b, which encode several NKBs, including NKBa-13, NKBa-10, NKBb-13, and NKBb-11, were cloned. Phylogenetic analysis revealed that NKBa-10 and NKBb-11 are closely related to mammalian NKB, while NKB-13s are more conserved in teleosts. Quantitative real-time PCR analyses in various tissues showed that TAC3a and TAC3b mRNAs were mainly expressed in the brain. In situ hybridization further detected TAC3a and TAC3b mRNAs in several regions of the brain known to be involved in the regulation of reproduction and metabolism, as well as in the neurohypophysis of the pituitary. To investigate the potential role of NKBs in reproduction, goldfish were injected intraperitoneally with synthetic NKBa-13, -10, NKBb-13, or -11 peptides and the mRNA levels of hypothalamic gonadotropin-releasing hormone (GnRH) and pituitary gonadotropin subunits were measured. NKBa-13, -10, or NKBb-13, but not -11, significantly increased hypothalamic salmon GnRH and pituitary FSHβ and LHβ mRNA levels in both female and male goldfish. Finally, ovariectomy increased, while estradiol replacement reduced, TAC3a mRNA levels without affecting TAC3b expression in the hypothalamus. These data suggest that NKBa-13, -10, and NKBb-13 play a role in mediating the estrogen negative feedback regulation of gonadotropins.

  • the evolution of tachykinin tachykinin receptor tac tacr in vertebrates and molecular identification of the TAC3 tacr3 system in zebrafish danio rerio
    Molecular and Cellular Endocrinology, 2012
    Co-Authors: Wenyi Zhou, Yun Liu, Huapu Chen, Christopher H K Cheng, Xiaochun Liu, Yong Zhang, Haoran Lin
    Abstract:

    Tachykinins are a family of peptides that are conserved from invertebrates to mammals. However, little is known about the evolutionary history of tachykinin (TAC) and tachykinin receptor (TACR) genes in vertebrates, especially in the teleost group. In the present study, five TACs and six TACRs genes were identified in the zebrafish genome. Genomic synteny analysis and phylogenetic tree analysis indicate that the increased numbers of TAC and TACR genes in vertebrates are the result of both genome duplications and local individual gene duplication. The full-length cDNA sequences encoding multiple TAC3s (TAC3a and TAC3b) and TACR3s (TACR3a1, TACR3a2 and TACR3b) were subsequently cloned from zebrafish brain samples. Sequence analysis suggested that four putative neurokinin B (NKB)-like peptides (NKBa-13, NKBa-10, NKBb-13 and NKBb-11) might be generated by the processing of two zebrafish TAC3 precursors. Tissue distribution studies in zebrafish revealed that TAC3 and TACR3 are mainly expressed in the brain regions. The biological activities of four zebrafish NKB peptides and three TACR3s were further examined using transcription reporter assays in cultured eukaryotic cells. All the synthetic NKB peptides were able to evoke the downstream signaling events of TACR3s with the exception of NKBb-11. These results indicated that the multiple TAC/TACR genes identified in vertebrates evolved from gene duplication events and that the TAC3/TACR3 systems also operate in the teleost group.

Sylvie Braillytabard - One of the best experts on this subject based on the ideXlab platform.

  • kisspeptin restores pulsatile lh secretion in patients with neurokinin b signaling deficiencies physiological pathophysiological and therapeutic implications
    Neuroendocrinology, 2013
    Co-Authors: Jacques Young, Jyothis T George, Bruno Francou, Sylvie Braillytabard, Jérôme Bouligand, Anne Guiochonmantel, Javier A Tello, Richard A Anderson, Robert P. Millar
    Abstract:

    Pulsatile gonadotropin-releasing hormone (GnRH) is crucial to normal reproductive function and abnormalities in pulse frequency give rise to reproductive dysfunction. Kisspeptin and neurokinin B (NKB), neuropeptides secreted by the same neuronal population in the ventral hypothalamus, have emerged recently as critical central regulators of GnRH and thus gonadotropin secretion. Patients with mutations resulting in loss of signaling by either of these neuroendocrine peptides fail to advance through puberty but the mechanisms mediating this remain unresolved. We report here that continuous kisspeptin infusion restores gonadotropin pulsatility in patients with loss-of-function mutations in NKB (TAC3) or its receptor (TAC3R), indicating that kisspeptin on its own is sufficient to stimulate pulsatile GnRH secretion. Moreover, our findings suggest that NKB action is proximal to kisspeptin in the reproductive neuroendocrine cascade regulating GnRH secretion, and may act as an autocrine modulator of kisspeptin secretion. The ability of continuous kisspeptin infusion to induce pulsatile gonadotropin secretion further indicates that GnRH neurons are able to set up pulsatile secretion in the absence of pulsatile exogenous kisspeptin.

  • normosmic congenital hypogonadotropic hypogonadism due to TAC3 tacr3 mutations characterization of neuroendocrine phenotypes and novel mutations
    PLOS ONE, 2011
    Co-Authors: Bruno Francou, Sylvie Braillytabard, Jérôme Bouligand, Philippe Chanson, Adela Voican, Larbi Amazit, Severine Trabado, Jerome Fagart, Geri Meduri, Pierre Lecomte
    Abstract:

    Context TAC3/TACR3 mutations have been reported in normosmic congenital hypogonadotropic hypogonadism (nCHH) (OMIM #146110). In the absence of animal models, studies of human neuroendocrine phenotypes associated with neurokinin B and NK3R receptor dysfunction can help to decipher the pathophysiology of this signaling pathway. Objective To evaluate the prevalence of TAC3/TACR3 mutations, characterize novel TACR3 mutations and to analyze neuroendocrine profiles in nCHH caused by deleterious TAC3/TACR3 biallelic mutations. Results From a cohort of 352 CHH, we selected 173 nCHH patients and identified nine patients carrying TAC3 or TACR3 variants (5.2%). We describe here 7 of these TACR3 variants (1 frameshift and 2 nonsense deleterious mutations and 4 missense variants) found in 5 subjects. Modeling and functional studies of the latter demonstrated the deleterious consequence of one missense mutation (Tyr267Asn) probably caused by the misfolding of the mutated NK3R protein. We found a statistically significant (p<0.0001) higher mean FSH/LH ratio in 11 nCHH patients with TAC3/TACR3 biallelic mutations than in 47 nCHH patients with either biallelic mutations in KISS1R, GNRHR, or with no identified mutations and than in 50 Kallmann patients with mutations in KAL1, FGFR1 or PROK2/PROKR2. Three patients with TAC3/TACR3 biallelic mutations had an apulsatile LH profile but low-frequency alpha-subunit pulses. Pulsatile GnRH administration increased alpha-subunit pulsatile frequency and reduced the FSH/LH ratio. Conclusion The gonadotropin axis dysfunction associated with nCHH due to TAC3/TACR3 mutations is related to a low GnRH pulsatile frequency leading to a low frequency of alpha-subunit pulses and to an elevated FSH/LH ratio. This ratio might be useful for pre-screening nCHH patients for TAC3/TACR3 mutations.

  • TAC3 and tacr3 defects cause hypothalamic congenital hypogonadotropic hypogonadism in humans
    The Journal of Clinical Endocrinology and Metabolism, 2010
    Co-Authors: Jacques Young, Bruno Francou, Jérôme Bouligand, Marie Laure Raffinsanson, Stephanie Gaillez, Marc Jeanpierre, Michael Grynberg, Peter Kamenicky, Philippe Chanson, Sylvie Braillytabard
    Abstract:

    Context: Missense loss-of-function mutations in TAC3 and TACR3, the genes encoding neurokinin B and its receptor NK3R, respectively, were recently discovered in kindreds with nonsyndromic normosmic congenital hypogonadotropic hypogonadism (CHH), thus identifying a fundamental role of this pathway in the human gonadotrope axis. Objective: The objective of the study was to investigate the consequences on gonadotrope axis of TAC3 deletion and TACR3 truncation in adult patients with normosmic complete CHH. Results: We identified three unrelated patients with the same homozygous substitution in the TAC3 intron 3 acceptor splicing site (c.209-1G>C) and three siblings who bore a homozygous mutation in the TACR3 intron 2 acceptor splicing site (c.738-1G>A). We demonstrated that these two mutations, respectively, deleted neurokinin B and truncated its receptor NK3R. We found in three patients with TAC3 mutation originating from Congo and Haiti a founding event in a more distant ancestor by means of haplotype analy...