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Pierre Dorny - One of the best experts on this subject based on the ideXlab platform.
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epidemiology of Taenia Saginata taeniosis cysticercosis in the russian federation
Parasites & Vectors, 2018Co-Authors: Branko Bobic, Lian F Thomas, Olgica Djurkovic Djakovic, Brecht Devleesschauwer, Veronique Dermauw, Pierre Dorny, Uffe Christian Braae, Lucy J RobertsonAbstract:Russia is traditionally an endemic area for Taenia Saginata infection, where a programme for the prevention of infection has been implemented for sixty years. This paper aims, therefore, to review the recent epidemiology data of Taenia Saginata infection in the Russian Federation. We undertook a systematic review of published and grey literature, and official data for information on the incidence, prevalence and distribution of Taenia Saginata taeniosis and cysticercosis in the Russian Federation between 1st January 1991 and 31st December 2017. From the 404 records returned by our search strategy, we identified 17 official county reports, 17 papers and one meeting abstract on the occurrence of taeniosis or cysticercosis from the Russian Federation, eligible for inclusion in this study. In the Russian Federation, Taenia Saginata infection has been continuously present and notifiable in the study period between 1991–2016. In the same area, a continuous decrease in the incidence of human taeniosis cases was observed, from 1.4 to 0.04 cases per 100,000 inhabitants, as well as a reduction in the territory where the infection is reported. The prevalence of bovine cysticercosis, ranging between 0.1–19.0%, generally has a declining trend, especially after 2005. Importance of Taenia Saginata infection as a medical and veterinary problem has been decreasing in the 21st century but it is still an infection with health and economic impact in the Russian Federation.
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epidemiology of Taenia Saginata taeniosis cysticercosis a systematic review of the distribution in the americas
Parasites & Vectors, 2018Co-Authors: Uffe Christian Braae, Lian F Thomas, Veronique Dermauw, Pierre Dorny, Lucy J Robertson, Arve Lee Willingham, Anastasios Saratsis, Brecht DevleesschauwerAbstract:The distribution of Taenia Saginata in the Americas is unclear. Establishing the distribution, economic burden, and potentials for control of bovine cysticercosis is increasingly important due to the growing demand for beef. This paper aims to take the first step and reviews the recent distribution of T. Saginata taeniosis and bovine cysticercosis on a national level within the Americas. We undertook a systematic review of published and grey literature for information on the occurrence, prevalence, and geographical distribution of bovine cysticercosis and human taeniosis in the 54 countries and territories of the Americas between January 1st, 1990 and December 31st, 2017. Data on bovine cysticercosis from OIE reports from 1994 to 2005 were also included. We identified 66 papers from the Americas with data on the occurrence of taeniosis or bovine cysticercosis and an additional 19 OIE country reports on bovine cysticercosis. Taeniosis was reported from 13 countries, with nine of these countries reporting specifically T. Saginata taeniosis, and four countries reporting non-species specific taeniosis. The reported prevalence of taeniosis ranged between 0.04–8.8%. Bovine cysticercosis was reported from 19 countries, nine identified through the literature search, and an additional 10 identified through the OIE country reports for notifiable diseases. The reported prevalence of bovine cysticercosis ranged between 0.1–19%. Disease occurrence was restricted to 21 countries within the Americas, the majority from the mainland, with the only island nations reporting either bovine cysticercosis or taeniosis being Cuba, Haiti, and the US Virgin Islands. Taenia Saginata is widely distributed across 21 of the 54 countries in the Americas, but insufficient epidemiological data are available to estimate the subnational spatial distribution, prevalence, incidence and intensity of infections. This needs to be addressed through active surveillance and disease detection programmes. Such programmes would improve the data quantity and quality, and may enable estimation of the economic burden due to bovine cysticercosis in the region in turn determining the requirement for and cost-effectiveness of control measures.
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Epidemiology of Taenia Saginata taeniosis/cysticercosis: a systematic review of the distribution in the Americas
'Springer Science and Business Media LLC', 2018Co-Authors: Uffe Christian Braae, Lian F Thomas, Veronique Dermauw, Pierre Dorny, Lucy J Robertson, Arve Lee Willingham, Anastasios Saratsis, Brecht DevleesschauwerAbstract:Abstract Background The distribution of Taenia Saginata in the Americas is unclear. Establishing the distribution, economic burden, and potentials for control of bovine cysticercosis is increasingly important due to the growing demand for beef. This paper aims to take the first step and reviews the recent distribution of T. Saginata taeniosis and bovine cysticercosis on a national level within the Americas. Methods We undertook a systematic review of published and grey literature for information on the occurrence, prevalence, and geographical distribution of bovine cysticercosis and human taeniosis in the 54 countries and territories of the Americas between January 1st, 1990 and December 31st, 2017. Data on bovine cysticercosis from OIE reports from 1994 to 2005 were also included. Results We identified 66 papers from the Americas with data on the occurrence of taeniosis or bovine cysticercosis and an additional 19 OIE country reports on bovine cysticercosis. Taeniosis was reported from 13 countries, with nine of these countries reporting specifically T. Saginata taeniosis, and four countries reporting non-species specific taeniosis. The reported prevalence of taeniosis ranged between 0.04–8.8%. Bovine cysticercosis was reported from 19 countries, nine identified through the literature search, and an additional 10 identified through the OIE country reports for notifiable diseases. The reported prevalence of bovine cysticercosis ranged between 0.1–19%. Disease occurrence was restricted to 21 countries within the Americas, the majority from the mainland, with the only island nations reporting either bovine cysticercosis or taeniosis being Cuba, Haiti, and the US Virgin Islands. Conclusions Taenia Saginata is widely distributed across 21 of the 54 countries in the Americas, but insufficient epidemiological data are available to estimate the subnational spatial distribution, prevalence, incidence and intensity of infections. This needs to be addressed through active surveillance and disease detection programmes. Such programmes would improve the data quantity and quality, and may enable estimation of the economic burden due to bovine cysticercosis in the region in turn determining the requirement for and cost-effectiveness of control measures
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investigation of an outbreak of Taenia Saginata cysts cysticercus bovis in dairy cattle from two farms
Veterinary Parasitology, 2011Co-Authors: A M J Mcfadden, D D Heath, C M Morley, Pierre DornyAbstract:Abstract The paper describes the epidemiological investigation carried out on two dairy farms with cattle infected with Taenia Saginata cysts. On the first affected farm it was estimated using Bayesian techniques that approximately 65% of 1400 mixed-age cattle were infected with Taenia Saginata cysts. The investigation aimed to determine potential exposure pathways of cattle to Taenia Saginata with a view to finding the human source of infection and to describe the epidemiology of the outbreak on the affected farms. In order to determine potential exposure pathways, investigation was centred on how feed or water could have been contaminated with eggs. The plausibility of pathways was determined by examining the spatial and temporal association between factors related to the pathway and the prevalence of infection in cattle strata. We describe the investigation carried out on affected farms.
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Taenia Saginata in europe
Veterinary Parasitology, 2007Co-Authors: Pierre Dorny, Nicolas PraetAbstract:In spite of the EU directives that regulate meat inspection for bovine cysticercosis, Taenia Saginata is still present in Europe and causes economic losses due to condemnation, refrigeration and downgrading of infected carcasses. The main reasons for this persistence include the low sensitivity of current meat inspection protocols, the dissemination and survival of eggs in the environment and cattle husbandry systems, which allow grazing on pastures and drinking from water streams. It is assumed that water streams and surface water are potentially contaminated with T. Saginata eggs. Furthermore, current wastewater management not only fails to halt, but rather contributes to the dissemination of eggs in the environment. Here, the authors discuss an integrated approach for control of this food-borne zoonosis, as well as the potential use of serological methods as a way of improving detection of bovine cysticercosis.
Julia Maria Costacruz - One of the best experts on this subject based on the ideXlab platform.
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jacalin unbound fraction of Taenia Saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid
Diagnostic Microbiology and Infectious Disease, 2010Co-Authors: Daniela Da Silva Nunes, Marianna Nascimento Manhani, Vanessa Da Silva Ribeiro, Julia Maria CostacruzAbstract:Abstract The aim of this study was to evaluate jacalin-bound fraction (JBF) and jacalin-unbound fraction (JUF) of the total saline extract from Taenia Saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose ® -jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39–42, 47–52, 64–68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. Saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC.
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a glance at Taenia Saginata infection diagnosis vaccine biological control and treatment
Infectious disorders drug targets, 2010Co-Authors: Claudio Vieira Da Silva, Julia Maria CostacruzAbstract:The Taenia Saginata Taeniasis-cysticercosis complex is a cosmopolitan zoonosis of great medical, veterinary and economic importance where humans play an important role as the carrier of adult stage and cattle as carrier of the larval stage of the parasite. Here we reviewed aspects concerning diagnosis, vaccine development, biological control and treatment of the disease.
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hydrophobic fraction of Taenia Saginata metacestodes rather than hydrophilic fraction contains immunodominant markers for diagnosing human neurocysticercosis
Revista Da Sociedade Brasileira De Medicina Tropical, 2010Co-Authors: F Goncalves, Gleyce Alves Machado, Heliana B Oliveira, Maria Teresa Nunes Pacheco Rezende, Jose Roberto Mineo, Julia Maria CostacruzAbstract:INTRODUCTION: Considering that alternative antigens for diagnosing neurocysticercosis continue to be a challenge because of the increasing difficulty in obtaining parasites from naturally infected pigs for preparation of Taenia solium homologous antigen, the aim of the present study was to evaluate the detergent (D) and aqueous (A) fractions from saline extract of Taenia Saginata metacestodes for diagnosing neurocysticercosis. METHODS: Taenia Saginata was obtained from naturally infected bovines in the Triângulo Mineiro region, State of Minas Gerais, Brazil. The carcasses came from cold storage units and had been slaughtered in accordance with the inspection technique recommended by the Federal Inspection Service. The D and A fractions were obtained by using Triton X-114 (TX-114). Serum samples were obtained from 40 patients with a diagnosis of neurocysticercosis, 45 with other parasitic diseases and 30 from apparently normal individuals. IgG antibody levels were evaluated using the ELISA and immunoblotting assays. RESULTS: The ELISA sensitivity and specificity were 95% and 73.3%, when using saline extract; 95% and 82.6% for the D fraction; and 65% and 61.3% for the A fraction, respectively. The immunoblotting assay confirmed the ELISA results, such that the D fraction was more efficient than the other extracts, and the 70-68kDa component was immunodominant among neurocysticercosis patients. CONCLUSIONS: These results demonstrated that the D fraction from Taenia Saginata metacestodes obtained using TX-114 can be used as a heterologous antigenic fraction in the immunoblotting assay for serologically diagnosing human neurocysticercosis, given its ability to select immunodominant antigens.
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parasitology jacalin unbound fraction of Taenia Saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid
2010Co-Authors: Daniela Da Silva Nunes, Marianna Nascimento Manhani, Vanessa Da Silva Ribeiro, Julia Maria CostacruzAbstract:The aim of this study was to evaluate jacalin-bound fraction (JBF) and jacalin-unbound fraction (JUF) of the total saline extract from Taenia Saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose ® -jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39–42, 47–52, 64–68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. Saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC. © 2010 Elsevier Inc. All rights reserved.
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parasitology jacalin unbound fraction of Taenia Saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid
2010Co-Authors: Daniela Da Silva Nunes, Marianna Nascimento Manhani, Vanessa Da Silva Ribeiro, Julia Maria CostacruzAbstract:The aim of this study was to evaluate jacalin-bound fraction (JBF) and jacalin-unbound fraction (JUF) of the total saline extract from Taenia Saginata metacestodes for human neurocysticercosis (NC) immunodiagnosis in cerebrospinal fluid. Total extract, JBF, and JUF were separated by affinity chromatography using Sepharose ® -jacalin and were tested in enzyme-linked immunosorbent assay (ELISA) and Western blotting (WB) to detect immunoglobulin G. In ELISA test, JUF showed the higher diagnostic efficiency and specificity indexes, 92% and 100%, respectively. In WB, 5 immunodominant proteins (39–42, 47–52, 64–68, 70, and 75 kDa) were detected when using JUF. In conclusion, the results achieved demonstrate that JUF, obtained from T. Saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC. © 2010 Elsevier Inc. All rights reserved.
Leslie J S Harrison - One of the best experts on this subject based on the ideXlab platform.
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differential diagnosis of Taenia Saginata and Taenia Saginata asiatica Taeniasis through pcr
Diagnostic Microbiology and Infectious Disease, 2004Co-Authors: Luis Miguel Gonzalez, Leslie J S Harrison, Estrella Montero, Nimit Morakote, S Puente, Jose Luis Diaz De Tuesta, Teresa Serra, Rogelio Lopezvelez, Donald P Mcmanus, Michael R E ParkhouseAbstract:New multiplex-PCR and PCR-linked restriction fragment length polymorphism protocols, derived from Taenia Saginata HDP2 DNA sequence, have been designed that allow the simultaneous and specific identification of T. Saginata and Taenia Saginata asiatica. Proglottids expelled from 20 different Spanish Taeniasis patients, previously diagnosed as T. Saginata by both morphological identification and multiplex HDP2-PCR, were also examined by the newly developed PCR protocols, and the original diagnosis of T. Saginata infection was confirmed. All of the 20 T. Saginata samples were negative in the T. Saginata asiatica-specific PCR. Three authentic T. Saginata asiatica samples were unambiguously identified as such in the T. Saginata asiatica PCR. These new protocols have immediate potential for the specific, sensitive, and rapid identification of T. Saginata asiatica and may assist in taxonomic studies.
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differential diagnosis of Taenia Saginata and Taenia Saginata asiatica Taeniasis through pcr
Diagnostic Microbiology and Infectious Disease, 2004Co-Authors: Luis Miguel Gonzalez, Leslie J S Harrison, Estrella Montero, Nimit Morakote, S Puente, Jose Luis Diaz De Tuesta, Teresa Serra, Rogelio Lopezvelez, Donald P Mcmanus, Michael R E ParkhouseAbstract:New multiplex-PCR and PCR-linked restriction fragment length polymorphism protocols, derived from Taenia Saginata HDP2 DNA sequence (AccN#AJ133740), have been designed that allow the simultaneous and specific identification of T. Saginata and Taenia Saginata asiatica. Proglottids expelled from 20 different Spanish Taeniasis patients, previously diagnosed as T. Saginata by both morphological identification and multiplex HDP2-PCR, were also examined by the newly developed PCR protocols, and the original diagnosis of T. Saginata infection was confirmed. All of the 20 T. Saginata samples were negative in the T. Saginata asiatica–specific PCR. Three authentic T. Saginata asiatica samples were unambiguously identified as such in the T. Saginata asiatica PCR. These new protocols have immediate potential for the specific, sensitive, and rapid identification of T. Saginata asiatica and may assist in taxonomic studies. © 2004 Elsevier Inc. All rights reserved.
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cloning and characterization of Taenia Saginata paramyosin cdna
Parasitology Research, 2003Co-Authors: Elizabeth Ferrer, Eva M Moyano, Denise Bryce, Mildred Fostercuevas, Iris Davila, Maria Milagros Cortez, Laura Benítez, Luis Miguel Gonzalez, Leslie J S HarrisonAbstract:A λZAP-express cDNA library of Taenia Saginata metacestodes was constructed. Antibody screening yielded a clone with an insert of 3,408 bp, an open reading frame of 2,589 bp, a deduced sequence of 863 amino acid and a molecular mass of 98.89 kDa. Alignments of the predicted amino acid sequence showed identity with paramyosins from several species: 98.8% with Taenia solium, 96.3% with Echinococcus.granulosus and about 70% with Schistosoma spp. The insert was expressed and purified. A collagen binding assay was performed which showed that T. Saginata GST-paramyosin retained this property in a dose-dependent manner. Problems were encountered due to high backgrounds in serological assays in the homologous T. Saginata system. However, the recombinant paramyosin was recognized by antibodies present in 31.6% of sera from T. solium seropositive cysticercosis patients and 100% of the sera from acute cysticercosis patients. The immunodominant epitope was the carboxyl-terminal fragment of the molecule.
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differential diagnosis of Taenia Saginata and Taenia solium infection by pcr
Journal of Clinical Microbiology, 2000Co-Authors: Luis Miguel Gonzalez, Leslie J S Harrison, Estrella Montero, R M E Parkhouse, Teresa GarateAbstract:We have designed species-specific oligonucleotides which permit the differential detection of two species of cestodes, Taenia Saginata and Taenia solium. The oligonucleotides contain sequences established for two previously reported, noncoding DNA fragments cloned from a genomic library of T. Saginata. The first, which is T. Saginata specific (fragment HDP1), is a repetitive sequence with a 53-bp monomeric unit repeated 24 times in direct tandem along the 1,272-bp fragment. From this sequence the two oligonucleotides that were selected (oligonucleotides PTs4F1 and PTs4R1) specifically amplified genomic DNA (gDNA) from T. Saginata but not T. solium or other related cestodes and had a sensitivity down to 10 pg of T. Saginata gDNA. The second DNA fragment (fragment HDP2; 3,954 bp) hybridized to both T. Saginata and T. solium DNAs and was not a repetitive sequence. Three oligonucleotides (oligonucleotides PTs7S35F1, PTs7S35F2, and PTs7S35R1) designed from the sequence of HDP2 allowed the differential amplification of gDNAs from T. Saginata, T. solium, and Echinococcus granulosus in a multiplex PCR, which exhibits a sensitivity of 10 pg.
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sequence and immunogenicity of the Taenia Saginata homologue of the major surface antigen of echinococcus spp
Parasitology Research, 1998Co-Authors: Laura Benítez, Leslie J S Harrison, Luis Miguel Gonzalez, R M E Parkhouse, Bruno Gottstein, Teresa GarateAbstract:A clone (R-Tso18) was isolated from a Taenia Saginata oncosphere cDNA library by screening with sera from rabbits immunised with oncosphere extract. It contained a full-length cDNA sequence of 1893 bp with an open reading frame of 1680 bp, corresponding to 559 amino acids with a deduced molecular mass of 65.173 kDa and an isoelectric point of 6.08. The R-Tso18 protein showed 80–84% nucleotide identity with the major protoscolex surface antigens of Echinococcus multilocularis (EM10) and E. granulosus (EG10). Preliminary immunogenicity studies employing the radio-labeled R-Tso18 protein in immune co-precipitation assays indicated sero-positivity for T. Saginata-infected calf sera (6/13), T. solium cysticercosis human (7/22) and pig (2/2) sera and E. multilocularis (6/10)- and E. granulosus (1/12)-infected human sera, whereas other helminth-infection sera were negative. As immuno-precipitation is a relatively insensitive assay, it was concluded that further studies on the diagnostic potential of the purified recombinant R-Tso18 antigen, or its peptides, are merited.
Lian F Thomas - One of the best experts on this subject based on the ideXlab platform.
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epidemiology of Taenia Saginata taeniosis cysticercosis in the russian federation
Parasites & Vectors, 2018Co-Authors: Branko Bobic, Lian F Thomas, Olgica Djurkovic Djakovic, Brecht Devleesschauwer, Veronique Dermauw, Pierre Dorny, Uffe Christian Braae, Lucy J RobertsonAbstract:Russia is traditionally an endemic area for Taenia Saginata infection, where a programme for the prevention of infection has been implemented for sixty years. This paper aims, therefore, to review the recent epidemiology data of Taenia Saginata infection in the Russian Federation. We undertook a systematic review of published and grey literature, and official data for information on the incidence, prevalence and distribution of Taenia Saginata taeniosis and cysticercosis in the Russian Federation between 1st January 1991 and 31st December 2017. From the 404 records returned by our search strategy, we identified 17 official county reports, 17 papers and one meeting abstract on the occurrence of taeniosis or cysticercosis from the Russian Federation, eligible for inclusion in this study. In the Russian Federation, Taenia Saginata infection has been continuously present and notifiable in the study period between 1991–2016. In the same area, a continuous decrease in the incidence of human taeniosis cases was observed, from 1.4 to 0.04 cases per 100,000 inhabitants, as well as a reduction in the territory where the infection is reported. The prevalence of bovine cysticercosis, ranging between 0.1–19.0%, generally has a declining trend, especially after 2005. Importance of Taenia Saginata infection as a medical and veterinary problem has been decreasing in the 21st century but it is still an infection with health and economic impact in the Russian Federation.
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epidemiology of Taenia Saginata taeniosis cysticercosis a systematic review of the distribution in the americas
Parasites & Vectors, 2018Co-Authors: Uffe Christian Braae, Lian F Thomas, Veronique Dermauw, Pierre Dorny, Lucy J Robertson, Arve Lee Willingham, Anastasios Saratsis, Brecht DevleesschauwerAbstract:The distribution of Taenia Saginata in the Americas is unclear. Establishing the distribution, economic burden, and potentials for control of bovine cysticercosis is increasingly important due to the growing demand for beef. This paper aims to take the first step and reviews the recent distribution of T. Saginata taeniosis and bovine cysticercosis on a national level within the Americas. We undertook a systematic review of published and grey literature for information on the occurrence, prevalence, and geographical distribution of bovine cysticercosis and human taeniosis in the 54 countries and territories of the Americas between January 1st, 1990 and December 31st, 2017. Data on bovine cysticercosis from OIE reports from 1994 to 2005 were also included. We identified 66 papers from the Americas with data on the occurrence of taeniosis or bovine cysticercosis and an additional 19 OIE country reports on bovine cysticercosis. Taeniosis was reported from 13 countries, with nine of these countries reporting specifically T. Saginata taeniosis, and four countries reporting non-species specific taeniosis. The reported prevalence of taeniosis ranged between 0.04–8.8%. Bovine cysticercosis was reported from 19 countries, nine identified through the literature search, and an additional 10 identified through the OIE country reports for notifiable diseases. The reported prevalence of bovine cysticercosis ranged between 0.1–19%. Disease occurrence was restricted to 21 countries within the Americas, the majority from the mainland, with the only island nations reporting either bovine cysticercosis or taeniosis being Cuba, Haiti, and the US Virgin Islands. Taenia Saginata is widely distributed across 21 of the 54 countries in the Americas, but insufficient epidemiological data are available to estimate the subnational spatial distribution, prevalence, incidence and intensity of infections. This needs to be addressed through active surveillance and disease detection programmes. Such programmes would improve the data quantity and quality, and may enable estimation of the economic burden due to bovine cysticercosis in the region in turn determining the requirement for and cost-effectiveness of control measures.
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Epidemiology of Taenia Saginata taeniosis/cysticercosis: a systematic review of the distribution in the Americas
'Springer Science and Business Media LLC', 2018Co-Authors: Uffe Christian Braae, Lian F Thomas, Veronique Dermauw, Pierre Dorny, Lucy J Robertson, Arve Lee Willingham, Anastasios Saratsis, Brecht DevleesschauwerAbstract:Abstract Background The distribution of Taenia Saginata in the Americas is unclear. Establishing the distribution, economic burden, and potentials for control of bovine cysticercosis is increasingly important due to the growing demand for beef. This paper aims to take the first step and reviews the recent distribution of T. Saginata taeniosis and bovine cysticercosis on a national level within the Americas. Methods We undertook a systematic review of published and grey literature for information on the occurrence, prevalence, and geographical distribution of bovine cysticercosis and human taeniosis in the 54 countries and territories of the Americas between January 1st, 1990 and December 31st, 2017. Data on bovine cysticercosis from OIE reports from 1994 to 2005 were also included. Results We identified 66 papers from the Americas with data on the occurrence of taeniosis or bovine cysticercosis and an additional 19 OIE country reports on bovine cysticercosis. Taeniosis was reported from 13 countries, with nine of these countries reporting specifically T. Saginata taeniosis, and four countries reporting non-species specific taeniosis. The reported prevalence of taeniosis ranged between 0.04–8.8%. Bovine cysticercosis was reported from 19 countries, nine identified through the literature search, and an additional 10 identified through the OIE country reports for notifiable diseases. The reported prevalence of bovine cysticercosis ranged between 0.1–19%. Disease occurrence was restricted to 21 countries within the Americas, the majority from the mainland, with the only island nations reporting either bovine cysticercosis or taeniosis being Cuba, Haiti, and the US Virgin Islands. Conclusions Taenia Saginata is widely distributed across 21 of the 54 countries in the Americas, but insufficient epidemiological data are available to estimate the subnational spatial distribution, prevalence, incidence and intensity of infections. This needs to be addressed through active surveillance and disease detection programmes. Such programmes would improve the data quantity and quality, and may enable estimation of the economic burden due to bovine cysticercosis in the region in turn determining the requirement for and cost-effectiveness of control measures
Luis Miguel Gonzalez - One of the best experts on this subject based on the ideXlab platform.
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differential diagnosis of Taenia Saginata and Taenia Saginata asiatica Taeniasis through pcr
Diagnostic Microbiology and Infectious Disease, 2004Co-Authors: Luis Miguel Gonzalez, Leslie J S Harrison, Estrella Montero, Nimit Morakote, S Puente, Jose Luis Diaz De Tuesta, Teresa Serra, Rogelio Lopezvelez, Donald P Mcmanus, Michael R E ParkhouseAbstract:New multiplex-PCR and PCR-linked restriction fragment length polymorphism protocols, derived from Taenia Saginata HDP2 DNA sequence (AccN#AJ133740), have been designed that allow the simultaneous and specific identification of T. Saginata and Taenia Saginata asiatica. Proglottids expelled from 20 different Spanish Taeniasis patients, previously diagnosed as T. Saginata by both morphological identification and multiplex HDP2-PCR, were also examined by the newly developed PCR protocols, and the original diagnosis of T. Saginata infection was confirmed. All of the 20 T. Saginata samples were negative in the T. Saginata asiatica–specific PCR. Three authentic T. Saginata asiatica samples were unambiguously identified as such in the T. Saginata asiatica PCR. These new protocols have immediate potential for the specific, sensitive, and rapid identification of T. Saginata asiatica and may assist in taxonomic studies. © 2004 Elsevier Inc. All rights reserved.
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differential diagnosis of Taenia Saginata and Taenia Saginata asiatica Taeniasis through pcr
Diagnostic Microbiology and Infectious Disease, 2004Co-Authors: Luis Miguel Gonzalez, Leslie J S Harrison, Estrella Montero, Nimit Morakote, S Puente, Jose Luis Diaz De Tuesta, Teresa Serra, Rogelio Lopezvelez, Donald P Mcmanus, Michael R E ParkhouseAbstract:New multiplex-PCR and PCR-linked restriction fragment length polymorphism protocols, derived from Taenia Saginata HDP2 DNA sequence, have been designed that allow the simultaneous and specific identification of T. Saginata and Taenia Saginata asiatica. Proglottids expelled from 20 different Spanish Taeniasis patients, previously diagnosed as T. Saginata by both morphological identification and multiplex HDP2-PCR, were also examined by the newly developed PCR protocols, and the original diagnosis of T. Saginata infection was confirmed. All of the 20 T. Saginata samples were negative in the T. Saginata asiatica-specific PCR. Three authentic T. Saginata asiatica samples were unambiguously identified as such in the T. Saginata asiatica PCR. These new protocols have immediate potential for the specific, sensitive, and rapid identification of T. Saginata asiatica and may assist in taxonomic studies.
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cloning and characterization of Taenia Saginata paramyosin cdna
Parasitology Research, 2003Co-Authors: Elizabeth Ferrer, Eva M Moyano, Denise Bryce, Mildred Fostercuevas, Iris Davila, Maria Milagros Cortez, Laura Benítez, Luis Miguel Gonzalez, Leslie J S HarrisonAbstract:A λZAP-express cDNA library of Taenia Saginata metacestodes was constructed. Antibody screening yielded a clone with an insert of 3,408 bp, an open reading frame of 2,589 bp, a deduced sequence of 863 amino acid and a molecular mass of 98.89 kDa. Alignments of the predicted amino acid sequence showed identity with paramyosins from several species: 98.8% with Taenia solium, 96.3% with Echinococcus.granulosus and about 70% with Schistosoma spp. The insert was expressed and purified. A collagen binding assay was performed which showed that T. Saginata GST-paramyosin retained this property in a dose-dependent manner. Problems were encountered due to high backgrounds in serological assays in the homologous T. Saginata system. However, the recombinant paramyosin was recognized by antibodies present in 31.6% of sera from T. solium seropositive cysticercosis patients and 100% of the sera from acute cysticercosis patients. The immunodominant epitope was the carboxyl-terminal fragment of the molecule.
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differential diagnosis of Taenia Saginata and Taenia solium infection by pcr
Journal of Clinical Microbiology, 2000Co-Authors: Luis Miguel Gonzalez, Leslie J S Harrison, Estrella Montero, R M E Parkhouse, Teresa GarateAbstract:We have designed species-specific oligonucleotides which permit the differential detection of two species of cestodes, Taenia Saginata and Taenia solium. The oligonucleotides contain sequences established for two previously reported, noncoding DNA fragments cloned from a genomic library of T. Saginata. The first, which is T. Saginata specific (fragment HDP1), is a repetitive sequence with a 53-bp monomeric unit repeated 24 times in direct tandem along the 1,272-bp fragment. From this sequence the two oligonucleotides that were selected (oligonucleotides PTs4F1 and PTs4R1) specifically amplified genomic DNA (gDNA) from T. Saginata but not T. solium or other related cestodes and had a sensitivity down to 10 pg of T. Saginata gDNA. The second DNA fragment (fragment HDP2; 3,954 bp) hybridized to both T. Saginata and T. solium DNAs and was not a repetitive sequence. Three oligonucleotides (oligonucleotides PTs7S35F1, PTs7S35F2, and PTs7S35R1) designed from the sequence of HDP2 allowed the differential amplification of gDNAs from T. Saginata, T. solium, and Echinococcus granulosus in a multiplex PCR, which exhibits a sensitivity of 10 pg.
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sequence and immunogenicity of the Taenia Saginata homologue of the major surface antigen of echinococcus spp
Parasitology Research, 1998Co-Authors: Laura Benítez, Leslie J S Harrison, Luis Miguel Gonzalez, R M E Parkhouse, Bruno Gottstein, Teresa GarateAbstract:A clone (R-Tso18) was isolated from a Taenia Saginata oncosphere cDNA library by screening with sera from rabbits immunised with oncosphere extract. It contained a full-length cDNA sequence of 1893 bp with an open reading frame of 1680 bp, corresponding to 559 amino acids with a deduced molecular mass of 65.173 kDa and an isoelectric point of 6.08. The R-Tso18 protein showed 80–84% nucleotide identity with the major protoscolex surface antigens of Echinococcus multilocularis (EM10) and E. granulosus (EG10). Preliminary immunogenicity studies employing the radio-labeled R-Tso18 protein in immune co-precipitation assays indicated sero-positivity for T. Saginata-infected calf sera (6/13), T. solium cysticercosis human (7/22) and pig (2/2) sera and E. multilocularis (6/10)- and E. granulosus (1/12)-infected human sera, whereas other helminth-infection sera were negative. As immuno-precipitation is a relatively insensitive assay, it was concluded that further studies on the diagnostic potential of the purified recombinant R-Tso18 antigen, or its peptides, are merited.
