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Carlo Romagnoli - One of the best experts on this subject based on the ideXlab platform.
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antifungal activity of Tagetes patula extracts on some phytopathogenic fungi ultrastructural evidence on pythium ultimum
Microbiological Research, 2004Co-Authors: Donatella Mares, B Tosi, Ferruccio Poli, Elisa Andreotti, Carlo RomagnoliAbstract:Abstract Methanol extract, obtained from Tagetes patula plant, was assayed against three phytopathogenic fungi: Botrytis cinerea, Fusarium moniliforme and Pythium ultimum . The antifungal activity was tested both in the dark and in the light, using two different lighting systems. The data showed that the extract proved to have a dose-dependent activity on all the fungi with a marked difference between treatments in the light than in the dark. Good growth inhibition was observed in fungi only when these were treated with the highest dose of the extract and irradiated, whereas the same dose gave only a modest inhibition when the experiment was conducted in the dark. At 5 and 10 μg/ml in the dark, growth increased. The results indicated that the presence of a luminous source enhances the antifungal activity, with small differences between UV-A and solar spectrum light. SEM and TEM observations on Pythium ultimum revealed that the Tagetes patula extract induced alterations on cell fungal membranes with a photoactivation mechanism possibly involving the production of free radicals and leading to a premature aging of the mycelium.
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Antifungal Activity of Tagetes patula Extracts
Pharmaceutical Biology, 2002Co-Authors: Donatella Mares, B Tosi, Carlo Romagnoli, Ferruccio PoliAbstract:In the present paper the methanol extracts obtained from 10 cultivars of Tagetes patula were assayed on two phytopathogenic fungi: Botrytis cinerea and Fusarium moniliforme. B. cinerea showed a high dose-dependent inhibition, with a marked difference between light and dark treatment. F. moniliforme seems to be a more resistant test that does not appear to be affected by the different treatment conditions (light-dark) even at the highest dose. However, it can be asserted that Tagetes patula is a possible source of antifungal substances and that thiophene activity is, in general, strongly increased by UV-A irradiation. During the experiment the method of chromatographic plates was used to evaluate plant extracts bioactivity. The obtained data indicate that it is a rapid method than can be used as an alternative to Petri dish tests.
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Antifungal effects of α-terthienyl from Tagetes patula on five dermatophytes
Phytotherapy Research, 1994Co-Authors: Carlo Romagnoli, Donatella Mares, Maria Palmira Fasulo, Alessandro BruniAbstract:Alpha-terthienyl (αT), a thiophene compound isolated from Tagetes patula (Asteraceae), exhibits antifungal activity towards five strains of dermatophytes (Trichophyton mentagrophytes, T. rubrum, T. violaceum, Epidermophyton floccosum, Microsporum cookei). αT plus UVA irradiation for 90 min acted as a fungistatic at concentrations between 6 μM and 24 μM. Between 1 and 10 days after irradiation, the fungal growth was reduced or arrested with marked responses for T. mentagrophytes, T. rubrum and M. cookei. The ultrastructural events that occur in Trichophyton mentagrophytes after treatment were studied by electron microscopy. In the dark, αT caused no important ultrastructural modifications within the hyphae. After UVA irradiation the photoactive compound caused damage to membranes of the nucleus, mitochondria and endoplasmic reticulum. Plasmolytic and autolytic changes resulted in plasma membrane breakage and in cell wall aberrations. UVA-activated αT would appear to target protein-rich membranes of the dermatophytes.
Donatella Mares - One of the best experts on this subject based on the ideXlab platform.
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antifungal activity of Tagetes patula extracts on some phytopathogenic fungi ultrastructural evidence on pythium ultimum
Microbiological Research, 2004Co-Authors: Donatella Mares, B Tosi, Ferruccio Poli, Elisa Andreotti, Carlo RomagnoliAbstract:Abstract Methanol extract, obtained from Tagetes patula plant, was assayed against three phytopathogenic fungi: Botrytis cinerea, Fusarium moniliforme and Pythium ultimum . The antifungal activity was tested both in the dark and in the light, using two different lighting systems. The data showed that the extract proved to have a dose-dependent activity on all the fungi with a marked difference between treatments in the light than in the dark. Good growth inhibition was observed in fungi only when these were treated with the highest dose of the extract and irradiated, whereas the same dose gave only a modest inhibition when the experiment was conducted in the dark. At 5 and 10 μg/ml in the dark, growth increased. The results indicated that the presence of a luminous source enhances the antifungal activity, with small differences between UV-A and solar spectrum light. SEM and TEM observations on Pythium ultimum revealed that the Tagetes patula extract induced alterations on cell fungal membranes with a photoactivation mechanism possibly involving the production of free radicals and leading to a premature aging of the mycelium.
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Antifungal Activity of Tagetes patula Extracts
Pharmaceutical Biology, 2002Co-Authors: Donatella Mares, B Tosi, Carlo Romagnoli, Ferruccio PoliAbstract:In the present paper the methanol extracts obtained from 10 cultivars of Tagetes patula were assayed on two phytopathogenic fungi: Botrytis cinerea and Fusarium moniliforme. B. cinerea showed a high dose-dependent inhibition, with a marked difference between light and dark treatment. F. moniliforme seems to be a more resistant test that does not appear to be affected by the different treatment conditions (light-dark) even at the highest dose. However, it can be asserted that Tagetes patula is a possible source of antifungal substances and that thiophene activity is, in general, strongly increased by UV-A irradiation. During the experiment the method of chromatographic plates was used to evaluate plant extracts bioactivity. The obtained data indicate that it is a rapid method than can be used as an alternative to Petri dish tests.
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Antifungal effects of α-terthienyl from Tagetes patula on five dermatophytes
Phytotherapy Research, 1994Co-Authors: Carlo Romagnoli, Donatella Mares, Maria Palmira Fasulo, Alessandro BruniAbstract:Alpha-terthienyl (αT), a thiophene compound isolated from Tagetes patula (Asteraceae), exhibits antifungal activity towards five strains of dermatophytes (Trichophyton mentagrophytes, T. rubrum, T. violaceum, Epidermophyton floccosum, Microsporum cookei). αT plus UVA irradiation for 90 min acted as a fungistatic at concentrations between 6 μM and 24 μM. Between 1 and 10 days after irradiation, the fungal growth was reduced or arrested with marked responses for T. mentagrophytes, T. rubrum and M. cookei. The ultrastructural events that occur in Trichophyton mentagrophytes after treatment were studied by electron microscopy. In the dark, αT caused no important ultrastructural modifications within the hyphae. After UVA irradiation the photoactive compound caused damage to membranes of the nucleus, mitochondria and endoplasmic reticulum. Plasmolytic and autolytic changes resulted in plasma membrane breakage and in cell wall aberrations. UVA-activated αT would appear to target protein-rich membranes of the dermatophytes.
B Tosi - One of the best experts on this subject based on the ideXlab platform.
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antifungal activity of Tagetes patula extracts on some phytopathogenic fungi ultrastructural evidence on pythium ultimum
Microbiological Research, 2004Co-Authors: Donatella Mares, B Tosi, Ferruccio Poli, Elisa Andreotti, Carlo RomagnoliAbstract:Abstract Methanol extract, obtained from Tagetes patula plant, was assayed against three phytopathogenic fungi: Botrytis cinerea, Fusarium moniliforme and Pythium ultimum . The antifungal activity was tested both in the dark and in the light, using two different lighting systems. The data showed that the extract proved to have a dose-dependent activity on all the fungi with a marked difference between treatments in the light than in the dark. Good growth inhibition was observed in fungi only when these were treated with the highest dose of the extract and irradiated, whereas the same dose gave only a modest inhibition when the experiment was conducted in the dark. At 5 and 10 μg/ml in the dark, growth increased. The results indicated that the presence of a luminous source enhances the antifungal activity, with small differences between UV-A and solar spectrum light. SEM and TEM observations on Pythium ultimum revealed that the Tagetes patula extract induced alterations on cell fungal membranes with a photoactivation mechanism possibly involving the production of free radicals and leading to a premature aging of the mycelium.
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Antifungal Activity of Tagetes patula Extracts
Pharmaceutical Biology, 2002Co-Authors: Donatella Mares, B Tosi, Carlo Romagnoli, Ferruccio PoliAbstract:In the present paper the methanol extracts obtained from 10 cultivars of Tagetes patula were assayed on two phytopathogenic fungi: Botrytis cinerea and Fusarium moniliforme. B. cinerea showed a high dose-dependent inhibition, with a marked difference between light and dark treatment. F. moniliforme seems to be a more resistant test that does not appear to be affected by the different treatment conditions (light-dark) even at the highest dose. However, it can be asserted that Tagetes patula is a possible source of antifungal substances and that thiophene activity is, in general, strongly increased by UV-A irradiation. During the experiment the method of chromatographic plates was used to evaluate plant extracts bioactivity. The obtained data indicate that it is a rapid method than can be used as an alternative to Petri dish tests.
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Production of Thiophenes in Calli and Suspension Cultures of Tagetes patula L. as Influenced by Light/Dark Succession.
1992Co-Authors: Ferruccio Poli, B TosiAbstract:Summary POLI F., Tosi B., DALL'OLIO G. & BRUNI A. 1992. Production of thiophenes in calli and suspension cultures of Tagetes patula L. as influenced by light/dark succession. — Phyton (Horn, Austria) 31 (2): 257-262, 3 figures. - English with German summary. HPTLC-densitometry was used to evaluate thiophene accumulation in leaf calli and cell suspension of Tagetes patula. Calli obtained from 7-8 weeks old leaves were subcultured in supplemented MS for many months. The secondary calli showed 4 to 6-fold higher fresh weight increment in 2 weeks while the tertiary ones only doubled their weight in the same period. A considerable increase of thiophene production (about 16.5 -fold) was obtained growing calli under successive light/dark periods. Repeated subculturing of callus tissues resulted in a slow decline of the thiophene yield. Under the same conditions, cell suspension cultures did not exhibit an appreciable increment in thiophene content which decreased with subculturing.
Ferruccio Poli - One of the best experts on this subject based on the ideXlab platform.
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antifungal activity of Tagetes patula extracts on some phytopathogenic fungi ultrastructural evidence on pythium ultimum
Microbiological Research, 2004Co-Authors: Donatella Mares, B Tosi, Ferruccio Poli, Elisa Andreotti, Carlo RomagnoliAbstract:Abstract Methanol extract, obtained from Tagetes patula plant, was assayed against three phytopathogenic fungi: Botrytis cinerea, Fusarium moniliforme and Pythium ultimum . The antifungal activity was tested both in the dark and in the light, using two different lighting systems. The data showed that the extract proved to have a dose-dependent activity on all the fungi with a marked difference between treatments in the light than in the dark. Good growth inhibition was observed in fungi only when these were treated with the highest dose of the extract and irradiated, whereas the same dose gave only a modest inhibition when the experiment was conducted in the dark. At 5 and 10 μg/ml in the dark, growth increased. The results indicated that the presence of a luminous source enhances the antifungal activity, with small differences between UV-A and solar spectrum light. SEM and TEM observations on Pythium ultimum revealed that the Tagetes patula extract induced alterations on cell fungal membranes with a photoactivation mechanism possibly involving the production of free radicals and leading to a premature aging of the mycelium.
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Antifungal Activity of Tagetes patula Extracts
Pharmaceutical Biology, 2002Co-Authors: Donatella Mares, B Tosi, Carlo Romagnoli, Ferruccio PoliAbstract:In the present paper the methanol extracts obtained from 10 cultivars of Tagetes patula were assayed on two phytopathogenic fungi: Botrytis cinerea and Fusarium moniliforme. B. cinerea showed a high dose-dependent inhibition, with a marked difference between light and dark treatment. F. moniliforme seems to be a more resistant test that does not appear to be affected by the different treatment conditions (light-dark) even at the highest dose. However, it can be asserted that Tagetes patula is a possible source of antifungal substances and that thiophene activity is, in general, strongly increased by UV-A irradiation. During the experiment the method of chromatographic plates was used to evaluate plant extracts bioactivity. The obtained data indicate that it is a rapid method than can be used as an alternative to Petri dish tests.
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Production of Thiophenes in Calli and Suspension Cultures of Tagetes patula L. as Influenced by Light/Dark Succession.
1992Co-Authors: Ferruccio Poli, B TosiAbstract:Summary POLI F., Tosi B., DALL'OLIO G. & BRUNI A. 1992. Production of thiophenes in calli and suspension cultures of Tagetes patula L. as influenced by light/dark succession. — Phyton (Horn, Austria) 31 (2): 257-262, 3 figures. - English with German summary. HPTLC-densitometry was used to evaluate thiophene accumulation in leaf calli and cell suspension of Tagetes patula. Calli obtained from 7-8 weeks old leaves were subcultured in supplemented MS for many months. The secondary calli showed 4 to 6-fold higher fresh weight increment in 2 weeks while the tertiary ones only doubled their weight in the same period. A considerable increase of thiophene production (about 16.5 -fold) was obtained growing calli under successive light/dark periods. Repeated subculturing of callus tissues resulted in a slow decline of the thiophene yield. Under the same conditions, cell suspension cultures did not exhibit an appreciable increment in thiophene content which decreased with subculturing.
Elisa Andreotti - One of the best experts on this subject based on the ideXlab platform.
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antifungal activity of Tagetes patula extracts on some phytopathogenic fungi ultrastructural evidence on pythium ultimum
Microbiological Research, 2004Co-Authors: Donatella Mares, B Tosi, Ferruccio Poli, Elisa Andreotti, Carlo RomagnoliAbstract:Abstract Methanol extract, obtained from Tagetes patula plant, was assayed against three phytopathogenic fungi: Botrytis cinerea, Fusarium moniliforme and Pythium ultimum . The antifungal activity was tested both in the dark and in the light, using two different lighting systems. The data showed that the extract proved to have a dose-dependent activity on all the fungi with a marked difference between treatments in the light than in the dark. Good growth inhibition was observed in fungi only when these were treated with the highest dose of the extract and irradiated, whereas the same dose gave only a modest inhibition when the experiment was conducted in the dark. At 5 and 10 μg/ml in the dark, growth increased. The results indicated that the presence of a luminous source enhances the antifungal activity, with small differences between UV-A and solar spectrum light. SEM and TEM observations on Pythium ultimum revealed that the Tagetes patula extract induced alterations on cell fungal membranes with a photoactivation mechanism possibly involving the production of free radicals and leading to a premature aging of the mycelium.
