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Daniele G. Machado - One of the best experts on this subject based on the ideXlab platform.
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antidepressant like effects of fractions essential oil carnosol and betulinic acid isolated from rosmarinus officinalis l
Food Chemistry, 2013Co-Authors: Daniele G. Machado, Vivian B Neis, Grasiela O Balen, Andre R S Colla, Agatha Oliveira, Francis L Pazini, Luis E.b. Bettio, Mauricio P. Cunha, Juliana Bastos Dalmarco, Edesio Luiz SimionattoAbstract:Abstract The aim of this study was to investigate the antidepressant-like effect of fractions from Rosmarinus officinalis L.: ethyl acetate 1 and 2 (AcOEt1 and 2), hexane (HEX), ethanolic (ET), and essential oil-free (EOF) fractions, as well as essential oil, the isolated compounds carnosol and betulinic acid in the Tail Suspension Test, a predictive Test of antidepressant activity. Swiss mice were acutely administered by oral route (p.o.) with fractions, essential oil or isolated compounds, 60 min before the Tail Suspension Test or open-field Test. All of them produced a significant antidepressant-like effect: AcOEt1, ET, EOF fractions and essential oil (0.1–100 mg/kg, p.o); HEX (0.1–10 mg/kg, p.o) and AcOEt2 fraction (0.1–1 mg/kg, p.o), carnosol (0.01–0.1 mg/kg, p.o.) isolated from the HEX fraction and betulinic acid (10 mg/kg, p.o.), isolated from the AcOEt1 and AcOEt2 fractions. No psychostimulant effect was shown in the open-field Test, indicating that the effects in the Tail Suspension Test are specific. This study suggests that carnosol and betulinic acid could be responsible for the anti-immobility effect of extracts from R. officinalis.
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antidepressant like effects of fractions essential oil carnosol and betulinic acid isolated from rosmarinus officinalis l
Food Chemistry, 2013Co-Authors: Daniele G. Machado, Vivian B Neis, Grasiela O Balen, Andre R S Colla, Agatha Oliveira, Francis L Pazini, Luis E.b. Bettio, Mauricio P. Cunha, Juliana Bastos Dalmarco, Edesio Luiz SimionattoAbstract:Abstract The aim of this study was to investigate the antidepressant-like effect of fractions from Rosmarinus officinalis L.: ethyl acetate 1 and 2 (AcOEt1 and 2), hexane (HEX), ethanolic (ET), and essential oil-free (EOF) fractions, as well as essential oil, the isolated compounds carnosol and betulinic acid in the Tail Suspension Test, a predictive Test of antidepressant activity. Swiss mice were acutely administered by oral route (p.o.) with fractions, essential oil or isolated compounds, 60 min before the Tail Suspension Test or open-field Test. All of them produced a significant antidepressant-like effect: AcOEt1, ET, EOF fractions and essential oil (0.1–100 mg/kg, p.o); HEX (0.1–10 mg/kg, p.o) and AcOEt2 fraction (0.1–1 mg/kg, p.o), carnosol (0.01–0.1 mg/kg, p.o.) isolated from the HEX fraction and betulinic acid (10 mg/kg, p.o.), isolated from the AcOEt1 and AcOEt2 fractions. No psychostimulant effect was shown in the open-field Test, indicating that the effects in the Tail Suspension Test are specific. This study suggests that carnosol and betulinic acid could be responsible for the anti-immobility effect of extracts from R. officinalis.
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antidepressant like effect of scopoletin a coumarin isolated from polygala sabulosa polygalaceae in mice evidence for the involvement of monoaminergic systems
European Journal of Pharmacology, 2010Co-Authors: Juliano C. Capra, Beatriz G Mendes, Daniele G. Machado, Mauricio P. Cunha, Andréa D.e. Zomkowski, Adair R.s. Santos, Moacir Geraldo Pizzolatti, Ana Lúcia S. RodriguesAbstract:Abstract The relationship between depression and monoaminergic systems has been hypothesized for many years. In this study, we have investigated the possible antidepressant-like effect of scopoletin, a coumarin from Polygala sabulosa in the Tail Suspension Test and forced swimming Test. Moreover, the ability of scopoletin to reverse the depression-like behavior in the forced swimming Test induced by immobility stress in mice was evaluated. Scopoletin reduced the immobility time in the Tail Suspension Test (10–100 mg/kg, p.o.), but not in the forced swimming Test. Fluoxetine (positive control) decreased the immobility time in the forced swimming and Tail Suspension Tests (20 mg/kg, p.o. and 10 mg/kg. p.o., respectively). Immobility stress caused an increase in the immobility time in the forced swimming Test (depression-like behavior), which was reversed by scopoletin (1–100 mg/kg, p.o.) and fluoxetine (10 mg/kg, p.o.). Scopoletin produced no psychostimulant effect in the open-field Test. The pretreatment of mice with ketanserin (5 mg/kg, i.p., a preferential 5-HT2A receptor antagonist), prazosin (1 mg/kg, i.p., an α1-adrenoceptor antagonist), yohimbine (1 mg/kg, i.p., an α2-adrenoceptor antagonist), haloperidol (0.2 mg/kg, i.p., a dopaminergic receptor antagonist), SCH23390 (0.05 mg/kg, s.c., a dopamine D1 receptor antagonist) or sulpiride (50 mg/kg, i.p., a dopamine D2 receptor antagonist), but not WAY100635 (0.1 mg/kg, s.c., a selective 5-HT1A receptor antagonist) prevented the antidepressant-like effect of scopoletin (10 mg/kg, p.o.) in the Tail Suspension Test. The results indicate that its antidepressant-like effect is dependent on the serotonergic (5-HT2A receptors), noradrenergic (α1- and α2-adrenoceptors) and dopaminergic (dopamine D1 and D2 receptors) systems.
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interaction of zinc with antidepressants in the Tail Suspension Test
Progress in Neuro-psychopharmacology & Biological Psychiatry, 2008Co-Authors: Mauricio P. Cunha, Luis E.b. Bettio, Daniele G. Machado, Juliano C. Capra, Ana Lúcia S. RodriguesAbstract:The antidepressant-like effect of zinc has been shown in several animal models of depression. In this study, zinc chloride (ZnCl2) was given alone or in combination with different classes of antidepressants by oral route (p.o.) to mice and the behavioral response in the Tail Suspension Test (TST), a predictive Test of antidepressant action, was investigated. ZnCl2 at a dose of 10 and 30 mg/kg, p.o., reduced the immobility time in the TST, without affecting the locomotor activity in open-field Test. The antidepressants fluoxetine, paroxetine, imipramine, desipramine and bupropion produced a significant reduction in the immobility time in TST at the doses of 10, 1, 1, 1 and 10 mg/kg, p.o., respectively. The combined treatment of sub-effective doses of ZnCl2 (1 mg/kg) with sub-effective doses of fluoxetine (5 mg/kg), paroxetine (0.1 mg/kg), desipramine (0.1 mg/kg), imipramine (0.1 mg/kg) or bupropion (1 mg/kg) induced a significant reduction in the immobility time in the TST when compared with the groups treated with ZnCl2 or with antidepressants alone. The treatment with sub-effective doses of ZnCl2 and antidepressants alone or in combination did not affect the locomotion in open-field Test, except that desipramine alone reduced the ambulation. The results first indicate that ZnCl2 administered by p.o. route produces an antidepressant-like effect in the TST. Moreover, synergistic effects of zinc with antidepressants were shown in the TST, suggesting that an improvement in the response to the antidepressant therapy occurs when zinc is combined with different classes of antidepressants.
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antidepressant like effect of rutin isolated from the ethanolic extract from schinus molle l in mice evidence for the involvement of the serotonergic and noradrenergic systems
European Journal of Pharmacology, 2008Co-Authors: Daniele G. Machado, Luis E.b. Bettio, Mauricio P. Cunha, Adair R.s. Santos, Moacir Geraldo Pizzolatti, Ines Maria Costa Brighente, Ana Lúcia S. RodriguesAbstract:Abstract We have recently shown that the hexanic extract from leaves of Schinus molle produces antidepressant-like effects in the Tail Suspension Test in mice. This study investigated the antidepressant-like effect of the ethanolic extract from aerial part of S. molle in the forced swimming Test and Tail Suspension Test in mice, two predictive models of depression. Moreover, we investigated the antidepressant potential of rutin, a flavonoid isolated from the ethanolic extract of this plant and the influence of the pretreatment with the inhibitors of serotonin or noradrenaline synthesis, p-chlorophenylalanine methyl ester (PCPA) and α-methyl-p-tyrosine (AMPT), respectively in the antidepressant-like effect of this flavonoid. The administration of the ethanolic extract produced a reduction in the immobility time in the Tail Suspension Test (dose range 600–1000 mg/kg, p.o.), but not in the forced swimming Test. It also produced a reduction in the ambulation in the open-field Test in mice not previously habituated to the arena, but no effect in the locomotor activity in mice previously habituated to the open-field. The administration of rutin reduced the immobility time in the Tail Suspension Test (0.3–3 mg/kg, p.o.), but not in the forced swimming Test, without producing alteration in the locomotor activity. In addition, pretreatment of mice with PCPA (100 mg/kg, i.p., for 4 consecutive days) or AMPT (100 mg/kg, i.p.) prevented the anti-immobility effect of rutin (0.3 mg/kg, p.o.) in the Tail Suspension Test. The results firstly indicated the antidepressant-like effect of the ethanolic extract of S. molle in the Tail Suspension Test may be dependent on the presence of rutin that likely exerts its antidepressant-like effect by increasing the availability of serotonin and noradrenaline in the synaptic cleft.
Mauricio P. Cunha - One of the best experts on this subject based on the ideXlab platform.
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antidepressant like effects of fractions essential oil carnosol and betulinic acid isolated from rosmarinus officinalis l
Food Chemistry, 2013Co-Authors: Daniele G. Machado, Vivian B Neis, Grasiela O Balen, Andre R S Colla, Agatha Oliveira, Francis L Pazini, Luis E.b. Bettio, Mauricio P. Cunha, Juliana Bastos Dalmarco, Edesio Luiz SimionattoAbstract:Abstract The aim of this study was to investigate the antidepressant-like effect of fractions from Rosmarinus officinalis L.: ethyl acetate 1 and 2 (AcOEt1 and 2), hexane (HEX), ethanolic (ET), and essential oil-free (EOF) fractions, as well as essential oil, the isolated compounds carnosol and betulinic acid in the Tail Suspension Test, a predictive Test of antidepressant activity. Swiss mice were acutely administered by oral route (p.o.) with fractions, essential oil or isolated compounds, 60 min before the Tail Suspension Test or open-field Test. All of them produced a significant antidepressant-like effect: AcOEt1, ET, EOF fractions and essential oil (0.1–100 mg/kg, p.o); HEX (0.1–10 mg/kg, p.o) and AcOEt2 fraction (0.1–1 mg/kg, p.o), carnosol (0.01–0.1 mg/kg, p.o.) isolated from the HEX fraction and betulinic acid (10 mg/kg, p.o.), isolated from the AcOEt1 and AcOEt2 fractions. No psychostimulant effect was shown in the open-field Test, indicating that the effects in the Tail Suspension Test are specific. This study suggests that carnosol and betulinic acid could be responsible for the anti-immobility effect of extracts from R. officinalis.
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antidepressant like effects of fractions essential oil carnosol and betulinic acid isolated from rosmarinus officinalis l
Food Chemistry, 2013Co-Authors: Daniele G. Machado, Vivian B Neis, Grasiela O Balen, Andre R S Colla, Agatha Oliveira, Francis L Pazini, Luis E.b. Bettio, Mauricio P. Cunha, Juliana Bastos Dalmarco, Edesio Luiz SimionattoAbstract:Abstract The aim of this study was to investigate the antidepressant-like effect of fractions from Rosmarinus officinalis L.: ethyl acetate 1 and 2 (AcOEt1 and 2), hexane (HEX), ethanolic (ET), and essential oil-free (EOF) fractions, as well as essential oil, the isolated compounds carnosol and betulinic acid in the Tail Suspension Test, a predictive Test of antidepressant activity. Swiss mice were acutely administered by oral route (p.o.) with fractions, essential oil or isolated compounds, 60 min before the Tail Suspension Test or open-field Test. All of them produced a significant antidepressant-like effect: AcOEt1, ET, EOF fractions and essential oil (0.1–100 mg/kg, p.o); HEX (0.1–10 mg/kg, p.o) and AcOEt2 fraction (0.1–1 mg/kg, p.o), carnosol (0.01–0.1 mg/kg, p.o.) isolated from the HEX fraction and betulinic acid (10 mg/kg, p.o.), isolated from the AcOEt1 and AcOEt2 fractions. No psychostimulant effect was shown in the open-field Test, indicating that the effects in the Tail Suspension Test are specific. This study suggests that carnosol and betulinic acid could be responsible for the anti-immobility effect of extracts from R. officinalis.
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antidepressant like effect of scopoletin a coumarin isolated from polygala sabulosa polygalaceae in mice evidence for the involvement of monoaminergic systems
European Journal of Pharmacology, 2010Co-Authors: Juliano C. Capra, Beatriz G Mendes, Daniele G. Machado, Mauricio P. Cunha, Andréa D.e. Zomkowski, Adair R.s. Santos, Moacir Geraldo Pizzolatti, Ana Lúcia S. RodriguesAbstract:Abstract The relationship between depression and monoaminergic systems has been hypothesized for many years. In this study, we have investigated the possible antidepressant-like effect of scopoletin, a coumarin from Polygala sabulosa in the Tail Suspension Test and forced swimming Test. Moreover, the ability of scopoletin to reverse the depression-like behavior in the forced swimming Test induced by immobility stress in mice was evaluated. Scopoletin reduced the immobility time in the Tail Suspension Test (10–100 mg/kg, p.o.), but not in the forced swimming Test. Fluoxetine (positive control) decreased the immobility time in the forced swimming and Tail Suspension Tests (20 mg/kg, p.o. and 10 mg/kg. p.o., respectively). Immobility stress caused an increase in the immobility time in the forced swimming Test (depression-like behavior), which was reversed by scopoletin (1–100 mg/kg, p.o.) and fluoxetine (10 mg/kg, p.o.). Scopoletin produced no psychostimulant effect in the open-field Test. The pretreatment of mice with ketanserin (5 mg/kg, i.p., a preferential 5-HT2A receptor antagonist), prazosin (1 mg/kg, i.p., an α1-adrenoceptor antagonist), yohimbine (1 mg/kg, i.p., an α2-adrenoceptor antagonist), haloperidol (0.2 mg/kg, i.p., a dopaminergic receptor antagonist), SCH23390 (0.05 mg/kg, s.c., a dopamine D1 receptor antagonist) or sulpiride (50 mg/kg, i.p., a dopamine D2 receptor antagonist), but not WAY100635 (0.1 mg/kg, s.c., a selective 5-HT1A receptor antagonist) prevented the antidepressant-like effect of scopoletin (10 mg/kg, p.o.) in the Tail Suspension Test. The results indicate that its antidepressant-like effect is dependent on the serotonergic (5-HT2A receptors), noradrenergic (α1- and α2-adrenoceptors) and dopaminergic (dopamine D1 and D2 receptors) systems.
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interaction of zinc with antidepressants in the Tail Suspension Test
Progress in Neuro-psychopharmacology & Biological Psychiatry, 2008Co-Authors: Mauricio P. Cunha, Luis E.b. Bettio, Daniele G. Machado, Juliano C. Capra, Ana Lúcia S. RodriguesAbstract:The antidepressant-like effect of zinc has been shown in several animal models of depression. In this study, zinc chloride (ZnCl2) was given alone or in combination with different classes of antidepressants by oral route (p.o.) to mice and the behavioral response in the Tail Suspension Test (TST), a predictive Test of antidepressant action, was investigated. ZnCl2 at a dose of 10 and 30 mg/kg, p.o., reduced the immobility time in the TST, without affecting the locomotor activity in open-field Test. The antidepressants fluoxetine, paroxetine, imipramine, desipramine and bupropion produced a significant reduction in the immobility time in TST at the doses of 10, 1, 1, 1 and 10 mg/kg, p.o., respectively. The combined treatment of sub-effective doses of ZnCl2 (1 mg/kg) with sub-effective doses of fluoxetine (5 mg/kg), paroxetine (0.1 mg/kg), desipramine (0.1 mg/kg), imipramine (0.1 mg/kg) or bupropion (1 mg/kg) induced a significant reduction in the immobility time in the TST when compared with the groups treated with ZnCl2 or with antidepressants alone. The treatment with sub-effective doses of ZnCl2 and antidepressants alone or in combination did not affect the locomotion in open-field Test, except that desipramine alone reduced the ambulation. The results first indicate that ZnCl2 administered by p.o. route produces an antidepressant-like effect in the TST. Moreover, synergistic effects of zinc with antidepressants were shown in the TST, suggesting that an improvement in the response to the antidepressant therapy occurs when zinc is combined with different classes of antidepressants.
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antidepressant like effect of rutin isolated from the ethanolic extract from schinus molle l in mice evidence for the involvement of the serotonergic and noradrenergic systems
European Journal of Pharmacology, 2008Co-Authors: Daniele G. Machado, Luis E.b. Bettio, Mauricio P. Cunha, Adair R.s. Santos, Moacir Geraldo Pizzolatti, Ines Maria Costa Brighente, Ana Lúcia S. RodriguesAbstract:Abstract We have recently shown that the hexanic extract from leaves of Schinus molle produces antidepressant-like effects in the Tail Suspension Test in mice. This study investigated the antidepressant-like effect of the ethanolic extract from aerial part of S. molle in the forced swimming Test and Tail Suspension Test in mice, two predictive models of depression. Moreover, we investigated the antidepressant potential of rutin, a flavonoid isolated from the ethanolic extract of this plant and the influence of the pretreatment with the inhibitors of serotonin or noradrenaline synthesis, p-chlorophenylalanine methyl ester (PCPA) and α-methyl-p-tyrosine (AMPT), respectively in the antidepressant-like effect of this flavonoid. The administration of the ethanolic extract produced a reduction in the immobility time in the Tail Suspension Test (dose range 600–1000 mg/kg, p.o.), but not in the forced swimming Test. It also produced a reduction in the ambulation in the open-field Test in mice not previously habituated to the arena, but no effect in the locomotor activity in mice previously habituated to the open-field. The administration of rutin reduced the immobility time in the Tail Suspension Test (0.3–3 mg/kg, p.o.), but not in the forced swimming Test, without producing alteration in the locomotor activity. In addition, pretreatment of mice with PCPA (100 mg/kg, i.p., for 4 consecutive days) or AMPT (100 mg/kg, i.p.) prevented the anti-immobility effect of rutin (0.3 mg/kg, p.o.) in the Tail Suspension Test. The results firstly indicated the antidepressant-like effect of the ethanolic extract of S. molle in the Tail Suspension Test may be dependent on the presence of rutin that likely exerts its antidepressant-like effect by increasing the availability of serotonin and noradrenaline in the synaptic cleft.
Ana Lúcia S. Rodrigues - One of the best experts on this subject based on the ideXlab platform.
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antidepressant like effect of scopoletin a coumarin isolated from polygala sabulosa polygalaceae in mice evidence for the involvement of monoaminergic systems
European Journal of Pharmacology, 2010Co-Authors: Juliano C. Capra, Beatriz G Mendes, Daniele G. Machado, Mauricio P. Cunha, Andréa D.e. Zomkowski, Adair R.s. Santos, Moacir Geraldo Pizzolatti, Ana Lúcia S. RodriguesAbstract:Abstract The relationship between depression and monoaminergic systems has been hypothesized for many years. In this study, we have investigated the possible antidepressant-like effect of scopoletin, a coumarin from Polygala sabulosa in the Tail Suspension Test and forced swimming Test. Moreover, the ability of scopoletin to reverse the depression-like behavior in the forced swimming Test induced by immobility stress in mice was evaluated. Scopoletin reduced the immobility time in the Tail Suspension Test (10–100 mg/kg, p.o.), but not in the forced swimming Test. Fluoxetine (positive control) decreased the immobility time in the forced swimming and Tail Suspension Tests (20 mg/kg, p.o. and 10 mg/kg. p.o., respectively). Immobility stress caused an increase in the immobility time in the forced swimming Test (depression-like behavior), which was reversed by scopoletin (1–100 mg/kg, p.o.) and fluoxetine (10 mg/kg, p.o.). Scopoletin produced no psychostimulant effect in the open-field Test. The pretreatment of mice with ketanserin (5 mg/kg, i.p., a preferential 5-HT2A receptor antagonist), prazosin (1 mg/kg, i.p., an α1-adrenoceptor antagonist), yohimbine (1 mg/kg, i.p., an α2-adrenoceptor antagonist), haloperidol (0.2 mg/kg, i.p., a dopaminergic receptor antagonist), SCH23390 (0.05 mg/kg, s.c., a dopamine D1 receptor antagonist) or sulpiride (50 mg/kg, i.p., a dopamine D2 receptor antagonist), but not WAY100635 (0.1 mg/kg, s.c., a selective 5-HT1A receptor antagonist) prevented the antidepressant-like effect of scopoletin (10 mg/kg, p.o.) in the Tail Suspension Test. The results indicate that its antidepressant-like effect is dependent on the serotonergic (5-HT2A receptors), noradrenergic (α1- and α2-adrenoceptors) and dopaminergic (dopamine D1 and D2 receptors) systems.
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antidepressant like effect of the organoselenium compound ebselen in mice evidence for the involvement of the monoaminergic system
European Journal of Pharmacology, 2009Co-Authors: Thais Posser, Manuella P Kaster, Sara Cristiane Barauna, Ana Lúcia S. Rodrigues, Joao Rocha, Rodrigo B LealAbstract:Abstract Ebselen [2-phenyl-1,2-benzisoselenazol-3(2H)-one] is a seleno-organic compound which possesses a potent antioxidant activity and has been shown to exert neuroprotective effects in vitro and in vivo in a variety of pro-oxidative insults. The present study investigates a possible antidepressant activity of ebselen using two predictive Tests for antidepressant activity in rodents: the forced swimming Test and Tail Suspension Test. Additionally, the mechanisms involved in the antidepressant-like effect of ebselen in mice were also assessed. Ebselen (10 mg/kg, s.c.) decreased the immobility time in the forced swimming Test without accompanying changes in ambulation in the open-field Test. In contrast, the administration of ebselen (10–30 mg/kg) did not produce any effect in the Tail Suspension Test. The anti-immobility effect of ebselen (10 mg/kg, s.c.) was not prevented by pre-treatment of mice with p -chlorophenylalanine (PCPA, 100 mg/kg, i.p., an inhibitor of serotonin synthesis, 4 consecutive days), NAN-190 (0.5 mg/kg, i.p., a serotonin 5-HT 1A receptor antagonist) or ketanserin (5 mg/kg, i.p., a serotonin 5-HT 2A/2C receptor antagonist). On the other hand, the pre-treatment of mice with prazosin (1 mg/kg, i.p., an α 1 -adrenoceptor antagonist), yohimbine (1 mg/kg, i.p., an α 2 -adrenoceptor antagonist), SCH23390 (0.05 mg/kg, s.c., a dopamine D 1 receptor antagonist) or sulpiride (50 mg/kg, i.p., a dopamine D 2 receptor antagonist) completely blocked the antidepressant-like effect of ebselen (10 mg/kg, s.c.) in the forced swimming Test. It may be concluded that ebselen produces an antidepressant-like effect in the forced swimming Test that seems to be dependent on its interaction with the noradrenergic and dopaminergic systems, but not with the serotonergic system.
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interaction of zinc with antidepressants in the Tail Suspension Test
Progress in Neuro-psychopharmacology & Biological Psychiatry, 2008Co-Authors: Mauricio P. Cunha, Luis E.b. Bettio, Daniele G. Machado, Juliano C. Capra, Ana Lúcia S. RodriguesAbstract:The antidepressant-like effect of zinc has been shown in several animal models of depression. In this study, zinc chloride (ZnCl2) was given alone or in combination with different classes of antidepressants by oral route (p.o.) to mice and the behavioral response in the Tail Suspension Test (TST), a predictive Test of antidepressant action, was investigated. ZnCl2 at a dose of 10 and 30 mg/kg, p.o., reduced the immobility time in the TST, without affecting the locomotor activity in open-field Test. The antidepressants fluoxetine, paroxetine, imipramine, desipramine and bupropion produced a significant reduction in the immobility time in TST at the doses of 10, 1, 1, 1 and 10 mg/kg, p.o., respectively. The combined treatment of sub-effective doses of ZnCl2 (1 mg/kg) with sub-effective doses of fluoxetine (5 mg/kg), paroxetine (0.1 mg/kg), desipramine (0.1 mg/kg), imipramine (0.1 mg/kg) or bupropion (1 mg/kg) induced a significant reduction in the immobility time in the TST when compared with the groups treated with ZnCl2 or with antidepressants alone. The treatment with sub-effective doses of ZnCl2 and antidepressants alone or in combination did not affect the locomotion in open-field Test, except that desipramine alone reduced the ambulation. The results first indicate that ZnCl2 administered by p.o. route produces an antidepressant-like effect in the TST. Moreover, synergistic effects of zinc with antidepressants were shown in the TST, suggesting that an improvement in the response to the antidepressant therapy occurs when zinc is combined with different classes of antidepressants.
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antidepressant like effect of rutin isolated from the ethanolic extract from schinus molle l in mice evidence for the involvement of the serotonergic and noradrenergic systems
European Journal of Pharmacology, 2008Co-Authors: Daniele G. Machado, Luis E.b. Bettio, Mauricio P. Cunha, Adair R.s. Santos, Moacir Geraldo Pizzolatti, Ines Maria Costa Brighente, Ana Lúcia S. RodriguesAbstract:Abstract We have recently shown that the hexanic extract from leaves of Schinus molle produces antidepressant-like effects in the Tail Suspension Test in mice. This study investigated the antidepressant-like effect of the ethanolic extract from aerial part of S. molle in the forced swimming Test and Tail Suspension Test in mice, two predictive models of depression. Moreover, we investigated the antidepressant potential of rutin, a flavonoid isolated from the ethanolic extract of this plant and the influence of the pretreatment with the inhibitors of serotonin or noradrenaline synthesis, p-chlorophenylalanine methyl ester (PCPA) and α-methyl-p-tyrosine (AMPT), respectively in the antidepressant-like effect of this flavonoid. The administration of the ethanolic extract produced a reduction in the immobility time in the Tail Suspension Test (dose range 600–1000 mg/kg, p.o.), but not in the forced swimming Test. It also produced a reduction in the ambulation in the open-field Test in mice not previously habituated to the arena, but no effect in the locomotor activity in mice previously habituated to the open-field. The administration of rutin reduced the immobility time in the Tail Suspension Test (0.3–3 mg/kg, p.o.), but not in the forced swimming Test, without producing alteration in the locomotor activity. In addition, pretreatment of mice with PCPA (100 mg/kg, i.p., for 4 consecutive days) or AMPT (100 mg/kg, i.p.) prevented the anti-immobility effect of rutin (0.3 mg/kg, p.o.) in the Tail Suspension Test. The results firstly indicated the antidepressant-like effect of the ethanolic extract of S. molle in the Tail Suspension Test may be dependent on the presence of rutin that likely exerts its antidepressant-like effect by increasing the availability of serotonin and noradrenaline in the synaptic cleft.
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putrescine produces antidepressant like effects in the forced swimming Test and in the Tail Suspension Test in mice
Progress in Neuro-psychopharmacology & Biological Psychiatry, 2006Co-Authors: Andréa D.e. Zomkowski, Adair R.s. Santos, Ana Lúcia S. RodriguesAbstract:Putrescine, a polyamine present at high concentrations in the mammalian brain, was suggested to play a role in the modulation of depression. Thus, this study investigated the effect of putrescine in the mouse forced swimming Test (FST) and in the Tail Suspension Test (TST), two models predictive of antidepressant activity. Putrescine significantly reduced the immobility time both in the FST and in the TST (dose range of 1-10 mg/kg, i.p.), without changing locomotion in an open-field. I.c.v. injection of putrescine (0.1-10 nmol/site) also reduced the immobility time in the FST and in the TST. The pretreatment of mice with arcaine (1 mg/kg, i.p., an antagonist of the polyamine-site of NMDA receptor) completely blocked the anti-immobility effect of putrescine (10 mg/kg, i.p.). A subeffective dose of putrescine (0.1 mg/kg, i.p.) produced a synergistic antidepressant-like effect with agmatine (0.001 mg/kg, i.p.) in the FST. Moreover, a subeffective dose of putrescine (0.01 nmol/site, i.c.v.) produced a synergistic antidepressant-like effect with arcaine (50 microg/site, i.c.v.). The results indicate that putrescine produces antidepressant-like effects in the FST that seems to be mediated through its interaction with the polyamine-site of NMDA receptors.
Edesio Luiz Simionatto - One of the best experts on this subject based on the ideXlab platform.
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antidepressant like effects of fractions essential oil carnosol and betulinic acid isolated from rosmarinus officinalis l
Food Chemistry, 2013Co-Authors: Daniele G. Machado, Vivian B Neis, Grasiela O Balen, Andre R S Colla, Agatha Oliveira, Francis L Pazini, Luis E.b. Bettio, Mauricio P. Cunha, Juliana Bastos Dalmarco, Edesio Luiz SimionattoAbstract:Abstract The aim of this study was to investigate the antidepressant-like effect of fractions from Rosmarinus officinalis L.: ethyl acetate 1 and 2 (AcOEt1 and 2), hexane (HEX), ethanolic (ET), and essential oil-free (EOF) fractions, as well as essential oil, the isolated compounds carnosol and betulinic acid in the Tail Suspension Test, a predictive Test of antidepressant activity. Swiss mice were acutely administered by oral route (p.o.) with fractions, essential oil or isolated compounds, 60 min before the Tail Suspension Test or open-field Test. All of them produced a significant antidepressant-like effect: AcOEt1, ET, EOF fractions and essential oil (0.1–100 mg/kg, p.o); HEX (0.1–10 mg/kg, p.o) and AcOEt2 fraction (0.1–1 mg/kg, p.o), carnosol (0.01–0.1 mg/kg, p.o.) isolated from the HEX fraction and betulinic acid (10 mg/kg, p.o.), isolated from the AcOEt1 and AcOEt2 fractions. No psychostimulant effect was shown in the open-field Test, indicating that the effects in the Tail Suspension Test are specific. This study suggests that carnosol and betulinic acid could be responsible for the anti-immobility effect of extracts from R. officinalis.
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antidepressant like effects of fractions essential oil carnosol and betulinic acid isolated from rosmarinus officinalis l
Food Chemistry, 2013Co-Authors: Daniele G. Machado, Vivian B Neis, Grasiela O Balen, Andre R S Colla, Agatha Oliveira, Francis L Pazini, Luis E.b. Bettio, Mauricio P. Cunha, Juliana Bastos Dalmarco, Edesio Luiz SimionattoAbstract:Abstract The aim of this study was to investigate the antidepressant-like effect of fractions from Rosmarinus officinalis L.: ethyl acetate 1 and 2 (AcOEt1 and 2), hexane (HEX), ethanolic (ET), and essential oil-free (EOF) fractions, as well as essential oil, the isolated compounds carnosol and betulinic acid in the Tail Suspension Test, a predictive Test of antidepressant activity. Swiss mice were acutely administered by oral route (p.o.) with fractions, essential oil or isolated compounds, 60 min before the Tail Suspension Test or open-field Test. All of them produced a significant antidepressant-like effect: AcOEt1, ET, EOF fractions and essential oil (0.1–100 mg/kg, p.o); HEX (0.1–10 mg/kg, p.o) and AcOEt2 fraction (0.1–1 mg/kg, p.o), carnosol (0.01–0.1 mg/kg, p.o.) isolated from the HEX fraction and betulinic acid (10 mg/kg, p.o.), isolated from the AcOEt1 and AcOEt2 fractions. No psychostimulant effect was shown in the open-field Test, indicating that the effects in the Tail Suspension Test are specific. This study suggests that carnosol and betulinic acid could be responsible for the anti-immobility effect of extracts from R. officinalis.
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involvement of dopamine d1 receptors and α1 adrenoceptors in the antidepressant like effect of chlorpheniramine in the mouse Tail Suspension Test
European Journal of Pharmacology, 2007Co-Authors: Shoko Hirano, Shigeo Miyata, Kenji Onodera, Junzo KameiAbstract:Abstract It has been reported that chlorpheniramine, a classical antihistamine, has antidepressant-like effects in animal models of depression. In this study, we examined the involvement of dopaminergic (dopamine D1 and dopamine D2 receptors), noradrenergic (α1- and β-adrenoceptors) and serotonergic (5-HT1A and 5-HT2 receptors) receptors in the antidepressant-like effect of chlorpheniramine in the mouse Tail Suspension Test. We also investigated the involvement of these monoamine receptors in the antidepressant-like effect of imipramine for comparison with the mechanisms of the effect of chlorpheniramine. Both imipramine and chlorpheniramine significantly reduced the duration of immobility in the Tail Suspension Test without affecting spontaneous locomotor activity in mice. The anti-immobility effect of imipramine (30 mg/kg, i.p.) was significantly antagonized by the selective dopamine D1 receptor antagonist SCH23390 but not by the other receptor antagonists. In contrast, the anti-immobility effect of chlorpheniramine was significantly inhibited by SCH23390 and the selective α1-adrenoceptor antagonist prazosin, but not by the other receptor antagonists. In conclusion, these results suggest that chlorpheniramine exerts an antidepressant-like effect in the mouse Tail Suspension Test that is mediated by at least the activation of dopamine D1 receptors and α1-adrenoceptors. In addition, the antidepressant-like effect of chlorpheniramine may be induced by several mechanisms that are different from those involved in the antidepressant-like effect of imipramine.
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effects of histamine h1 receptor antagonists on depressive like behavior in diabetic mice
Pharmacology Biochemistry and Behavior, 2006Co-Authors: Shoko Hirano, Shigeo Miyata, Kenji Onodera, Junzo KameiAbstract:We previously reported that streptozotocin-induced diabetic mice showed depressive-like behavior in the Tail Suspension Test. It is well known that the central histaminergic system regulates many physiological functions including emotional behaviors. In this study, we examined the role of the central histaminergic system in the diabetes-induced depressive-like behavior in the mouse Tail Suspension Test. The histamine contents in the hypothalamus were significantly higher in diabetic mice than in non-diabetic mice. The histamine H(1) receptor antagonist chlorpheniramine (1-10 mg/kg, s.c.) dose-dependently and significantly reduced the duration of immobility in both non-diabetic and diabetic mice. In contrast, the selective histamine H(1) receptor antagonists epinastine (0.03-0.3 microg/mouse, i.c.v.) and cetirizine (0.01-0.1 microg/mouse, i.c.v.) dose-dependently and significantly suppressed the duration of immobility in diabetic mice, but not in non-diabetic mice. Spontaneous locomotor activity was not affected by histamine H(1) receptor antagonists in either non-diabetic or diabetic mice. In addition, the number and affinity of histamine H(1) receptors in the frontal cortex were not affected by diabetes. In conclusion, we suggest that the altered neuronal system mediated by the activation of histamine H(1) receptors is involved, at least in part, in the depressive-like behavior seen in diabetic mice.
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effects of selective serotonin reuptake inhibitors on immobility time in the Tail Suspension Test in streptozotocin induced diabetic mice
Pharmacology Biochemistry and Behavior, 2003Co-Authors: Junzo Kamei, Akiyoshi Saitoh, Kayo Morita, Shigeo Miyata, Hiroshi TakedaAbstract:Abstract We examined the effects of fluoxetine and fluvoxamine, selective serotonin reuptake inhibitors (SSRIs), and desipramine, a selective noradrenaline (NA) reuptake inhibitor, given alone or in combination with diazepam on immobility time in the Tail Suspension Test in diabetic mice. Immobility time was significantly longer in diabetic than in nondiabetic mice. Diazepam (0.1 and 0.3 mg/kg sc) dose-dependently decreased immobility time in diabetic mice to the level observed in saline-treated nondiabetic mice. However, diazepam had no significant effect on immobility time in nondiabetic mice. Fluoxetine (3–56 mg/kg ip) and desipramine (1–30 mg/kg ip) produced marked, dose-dependent suppression of immobility time in both nondiabetic and diabetic mice. However, anti-immobility effects of fluoxetine and desipramine in diabetic mice were less than those in nondiabetic mice. Fluvoxamine (3–30 mg/kg ip) produced a dose-dependent suppression of immobility time in nondiabetic mice but not in diabetic mice. The anti-immobility effects of fluoxetine, fluvoxamine and desipramine in nondiabetic mice were antagonized by pretreatment with diazepam (0.3 mg/kg sc). Furthermore, fluoxetine, fluvoxamine and desipramine had no effect on the immobility time in diazepam (0.3 mg/kg sc)-treated diabetic mice. These results indicate that the anti-immobility effects of SSRIs and desipramine are less in diabetic mice than in nondiabetic mice in the Tail Suspension Test. Furthermore, in diabetic mice, SSRIs and selective NA reuptake inhibitors did not affect immobility time even though the prolonged duration of immobility was suppressed by pretreatment with diazepam.
