The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Eric W. Kaler - One of the best experts on this subject based on the ideXlab platform.
-
Effects of bile salts on cholestan-3β,5α,6β-triol-induced apoptosis in dog gallbladder epithelial cells
Biochimica et Biophysica Acta, 2001Co-Authors: Tadashi Yoshida, J.henriëtte Klinkspoor, Rahul Kuver, Martin Poot, Peter S. Rabinovitch, Steven P. Wrenn, Eric W. KalerAbstract:Abstract Oxysterols are cytotoxic agents. The gallbladder epithelium is exposed to high concentrations of oxysterols, and so elucidating the mechanisms of cytotoxicity in this organ may enhance our understanding of the pathogenesis of biliary tract disorders. We investigated the cytotoxic effects of the oxysterol cholestan-3β,5α,6β-triol (TriolC) on dog gallbladder epithelial cells. Apoptosis was the major form of cytotoxicity, as determined by analysis of nuclear morphologic changes and by multiparameter flow cytometry. Hydrophobic bile salts are known to have cytotoxic effects, whereas hydrophilic bile salts have cytoprotective effects. We therefore examined whether the hydrophobic bile Acid Taurodeoxycholic Acid (TDC) and the hydrophilic bile Acid tauroursodeoxycholic Acid (TUDC) had modifying effects on oxysterol-induced cytotoxicity. TriolC caused an increase in the number of apoptotic cells from 14±11% (control) to 48±12% of total cells (P
-
Effects of bile salts on cholestan-3beta,5alpha,6beta-triol-induced apoptosis in dog gallbladder epithelial cells.
Biochimica et biophysica acta, 2001Co-Authors: Tadashi Yoshida, J.henriëtte Klinkspoor, Rahul Kuver, Martin Poot, Peter S. Rabinovitch, Steven P. Wrenn, Eric W. KalerAbstract:Oxysterols are cytotoxic agents. The gallbladder epithelium is exposed to high concentrations of oxysterols, and so elucidating the mechanisms of cytotoxicity in this organ may enhance our understanding of the pathogenesis of biliary tract disorders. We investigated the cytotoxic effects of the oxysterol cholestan-3beta,5alpha,6beta-triol (TriolC) on dog gallbladder epithelial cells. Apoptosis was the major form of cytotoxicity, as determined by analysis of nuclear morphologic changes and by multiparameter flow cytometry. Hydrophobic bile salts are known to have cytotoxic effects, whereas hydrophilic bile salts have cytoprotective effects. We therefore examined whether the hydrophobic bile Acid Taurodeoxycholic Acid (TDC) and the hydrophilic bile Acid tauroursodeoxycholic Acid (TUDC) had modifying effects on oxysterol-induced cytotoxicity. TriolC caused an increase in the number of apoptotic cells from 14+/-11% (control) to 48+/-12% of total cells (P
Tadashi Yoshida - One of the best experts on this subject based on the ideXlab platform.
-
Effects of bile salts on cholestan-3β,5α,6β-triol-induced apoptosis in dog gallbladder epithelial cells
Biochimica et Biophysica Acta, 2001Co-Authors: Tadashi Yoshida, J.henriëtte Klinkspoor, Rahul Kuver, Martin Poot, Peter S. Rabinovitch, Steven P. Wrenn, Eric W. KalerAbstract:Abstract Oxysterols are cytotoxic agents. The gallbladder epithelium is exposed to high concentrations of oxysterols, and so elucidating the mechanisms of cytotoxicity in this organ may enhance our understanding of the pathogenesis of biliary tract disorders. We investigated the cytotoxic effects of the oxysterol cholestan-3β,5α,6β-triol (TriolC) on dog gallbladder epithelial cells. Apoptosis was the major form of cytotoxicity, as determined by analysis of nuclear morphologic changes and by multiparameter flow cytometry. Hydrophobic bile salts are known to have cytotoxic effects, whereas hydrophilic bile salts have cytoprotective effects. We therefore examined whether the hydrophobic bile Acid Taurodeoxycholic Acid (TDC) and the hydrophilic bile Acid tauroursodeoxycholic Acid (TUDC) had modifying effects on oxysterol-induced cytotoxicity. TriolC caused an increase in the number of apoptotic cells from 14±11% (control) to 48±12% of total cells (P
-
Effects of bile salts on cholestan-3beta,5alpha,6beta-triol-induced apoptosis in dog gallbladder epithelial cells.
Biochimica et biophysica acta, 2001Co-Authors: Tadashi Yoshida, J.henriëtte Klinkspoor, Rahul Kuver, Martin Poot, Peter S. Rabinovitch, Steven P. Wrenn, Eric W. KalerAbstract:Oxysterols are cytotoxic agents. The gallbladder epithelium is exposed to high concentrations of oxysterols, and so elucidating the mechanisms of cytotoxicity in this organ may enhance our understanding of the pathogenesis of biliary tract disorders. We investigated the cytotoxic effects of the oxysterol cholestan-3beta,5alpha,6beta-triol (TriolC) on dog gallbladder epithelial cells. Apoptosis was the major form of cytotoxicity, as determined by analysis of nuclear morphologic changes and by multiparameter flow cytometry. Hydrophobic bile salts are known to have cytotoxic effects, whereas hydrophilic bile salts have cytoprotective effects. We therefore examined whether the hydrophobic bile Acid Taurodeoxycholic Acid (TDC) and the hydrophilic bile Acid tauroursodeoxycholic Acid (TUDC) had modifying effects on oxysterol-induced cytotoxicity. TriolC caused an increase in the number of apoptotic cells from 14+/-11% (control) to 48+/-12% of total cells (P
Guowang Xu - One of the best experts on this subject based on the ideXlab platform.
-
Metabonomics study of intestinal fistulas based on ultraperformance liquid chromatography coupled with Q-TOF mass spectrometry (UPLC/Q-TOF MS)
Journal of Proteome Research, 2006Co-Authors: Xinjie Zhao, Jiangshan Wang, Qiurong Li, Jieshou Li, Guowang XuAbstract:Ultraperformance liquid chromatography coupled with Q-TOF mass spectrometry (UPLC/Q-TOF MS) is an effective and sensitive analytical tool. A UPLC/Q-TOF MS-based metabonomics technique was employed to investigate sera from 40 patients with intestinal fistula and 17 healthy volunteers in an effort to find potential biomarkers of the disease and reveal their pathophysiological changes. After the UPLC/Q-TOF analysis, the retention time and m/z data pair for each peak were detected. Partial least squares discriminant analysis (PLS-DA) and coefficient of correlation analysis were used for marker selection and identification. According to the data, nine potential biomarkers were identified: glycochenodeoxycholic Acid, glycodeoxycholic Acid, taurochenodexycholic Acid, Taurodeoxycholic Acid, and two kinds of lysophosphatidyl choline (C16:0 and C18:2) were found with increased concentrations in the patients, and phenylalanine, tryptophan, and carnitine were found with decreased concentrations in the patients. The ...
Adrien A. Eshraghi - One of the best experts on this subject based on the ideXlab platform.
-
Evaluating the Efficacy of Taurodeoxycholic Acid in Providing Otoprotection Using an in vitro Model of Electrode Insertion Trauma.
Frontiers in molecular neuroscience, 2020Co-Authors: Viraj Shah, Rahul Mittal, David Shahal, Priyanka Sinha, Erdogan Bulut, Jeenu Mittal, Adrien A. EshraghiAbstract:Cochlear implants (CIs) are widely used to provide auditory rehabilitation to individuals having severe to profound sensorineural hearing loss (SNHL). However, insertion of electrode leads to inner trauma and activation of inflammatory and apoptotic signaling cascades resulting in loss of residual hearing in implanted individuals. Pharmaceutical interventions that can target these signaling cascades hold great potential for preserving residual hearing by preventing sensory cell damage. Bile salts have shown efficacy in various regions of the body as powerful antioxidants and anti-inflammatory agents. However, their efficacy against inner ear trauma has never been explored. The objective of this study was to determine whether Taurodeoxycholic Acid (TDCA), a bile salt derivative, can prevent sensory cell damage employing an in vitro model of electrode insertion trauma (EIT). The organ of Corti (OC) explants were dissected from postnatal day 3 (P-3) rats and placed in serum-free media. Explants were divided into control and experimental groups: (1) untreated controls; (2) EIT; (3) EIT+ TDCA (different concentrations). Hair cell (HC) density, analyses of apoptosis pathway (cleaved caspase 3), levels of reactive oxygen species (ROS) as well as inducible nitric oxide synthase (iNOS) activity and Mitochondrial Membrane Potential (MMP) were assayed. Treatment with TDCA provided significant otoprotection against HC loss in a dose-dependent manner. The molecular mechanisms underlying otoprotection involved decreasing oxidative stress, lowering levels of iNOS, and abrogating generation of cleaved caspase 3. The results of the present study suggest that TDCA provides efficient otoprotection against EIT, in vitro and should be explored for developing pharmaceutical interventions to preserve residual hearing post-cochlear implantation.
Alfredo Sanz-medel - One of the best experts on this subject based on the ideXlab platform.
-
Enantiomeric separation of selenoaminoAcid derivatives by cyclodextrin-modified micellar electrokinetic chromatography using a mixed micellar system of sodium dodecyl sulphate and Taurodeoxycholic Acid
Analytica Chimica Acta, 2000Co-Authors: S. Pérez Méndez, E. Blanco González, Alfredo Sanz-medelAbstract:Abstract A new cyclodextrin-modified micellar electrokinetic chromatography (CD - MEKC) method for the enantiomeric separation and determination of the selenoaminoAcids selenomethionine and selenoethionine, derivatised with 2,3-naphthalenedicarboxaldehyde (NDA) to produce cyanobenzoisoindole (CBI) derivatives, has been developed. Optical resolution was achieved in a fused-silica capillary column using 30 mmol l −1 phosphate/10 mmol l −1 boric Acid (pH 7) electrophoretic buffer containing a mixed micellar system of achiral sodium dodecyl sulphate and chiral Taurodeoxycholic Acid surfactants among with β-cyclodextrins. Detection was followed by direct UV absorptiometric measurements at 230 nm. The concentration influence of the two chiral selectors used (β-CD and TDC micelles), the effect of applied voltage and the pH of the electrophoretic buffer on the migration time and resolution have been investigated. The analytical performance of the method is discussed in terms of detection limits, linearity of response and precision. The method developed has been applied to the determination of enantiomeric purity in a commercial sample sold as ‘pure’ l -selenomethionine.
