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Chungjan Chang - One of the best experts on this subject based on the ideXlab platform.

  • xylella fastidiosa subspecies x fastidiosa subsp correction fastidiosa correction subsp nov x fastidiosa subsp multiplex subsp nov and x fastidiosa subsp pauca subsp nov
    Systematic and Applied Microbiology, 2004
    Co-Authors: Norman W Schaad, Elena Postnikova, George H Lacy, Mbarek Fatmi, Chungjan Chang
    Abstract:

    Xylella fastidiosa, a fastidious bacterium causing disease in over 100 plant species, is classified as a single species, although genetic studies support multiple Taxons. To determine the Taxonomic relatedness among strains of X. fastidiosa, we conducted DNA-DNA relatedness assays and sequenced the 16S-23S intergenic spacer (ITS) region using 26 strains from 10 hosts. Under stringent conditions (Tm -15 degrees C), the DNA relatedness for most X. fastidiosa strains was *70%. However, at high stringency (Tm -8 degrees C), three distinct genotypes (A, B, and C) were revealed. Taxon A included strains from cultivated grape, alfalfa, almond (two), and maple, interrelated by 85% (mean); Taxon B included strains from peach, elm, plum, pigeon grape, sycamore, and almond (one), interrelated by 84%; and Taxon C included only strains from citrus, interrelated by 87%. The mean reciprocal relatedness between Taxons A and B, A and C, and B and C, were 58, 41, and 45%, respectively. ITS results also indicated the same grouping; Taxons A and B, A and C, and B and C had identities of 98.7, 97.9, and 99.2%, respectively. Previous and present phenotypic data supports the molecular data. Taxon A strains grow faster on Pierce's disease agar medium whereas B and C strains grow more slowly. Taxon B and C strains are susceptible to penicillin and resistant to carbenicillin whereas A strains are opposite. Each Taxon can be differentiated serologically as well as by structural proteins. We propose Taxons A, B, and C be named X. fastidiosa subsp. fastidiosa [correction] subsp. nov, subsp. multiplex, subsp. nov., and subsp. pauca, subsp. nov., respectively. The type strains of the subspecies are subsp. fastidiosa [correction] ICPB 50025 (= ATTC 35879T and ICMP 15197), subsp. multiplex ICPB 50039 (= ATTC 35871 and ICMP 15199), and subsp. pauca ICPB 50031 (= ICMP 15198).

  • xylella fastidiosa subspecies x fastidiosa subsp piercei subsp nov x fastidiosa subsp multiplex subsp nov and x fastidiosa subsp pauca subsp nov
    Systematic and Applied Microbiology, 2004
    Co-Authors: Norman W Schaad, Elena Postnikova, George H Lacy, Mbarek Fatmi, Chungjan Chang
    Abstract:

    Summary Xylella fastidiosa, a fastidious bacterium causing disease in over 100 plant species, is classified as a single species, although genetic studies support multiple Taxons. To determine the Taxonomic relatedness among strains of X. fastidiosa, we conducted DNA-DNA relatedness assays and sequenced the 16S‐23S intergenic spacer (ITS) region using 26 strains from 10 hosts. Under stringent conditions (Tm ‐15 °C), the DNA relatedness for most X. fastidiosa strains was ≥70%. However, at high stringency (Tm ‐8 °C), three distinct genotypes (A, B, and C) were revealed. Taxon A included strains from cultivated grape, alfalfa, almond (two), and maple, interrelated by 85% (mean); Taxon B included strains from peach, elm, plum, pigeon grape, sycamore, and almond (one), interrelated by 84%; and Taxon C included only strains from citrus, interrelated by 87%. The mean reciprocal relatedness between Taxons A and B, A and C, and B and C, were 58, 41, and 45%, respectively. ITS results also indicated the same grouping ; Taxons A and B, A and C, and B and C had identities of 98.7, 97.9, and 99.2%, respectively. Previous and present phenotypic data supports the molecular data. Taxon A strains grow faster on Pierce’s disease agar medium whereas B and C strains grow more slowly. Taxon B and C strains are susceptible to penicillin and resistant to carbenicillin whereas A strains are opposite. Each Taxon can be differentiated serologically as well as by structural proteins. We propose Taxons A, B, and C be named X. fastidiosa subsp. piercei, subsp. nov, subsp. multiplex, subsp. nov., and subsp. pauca, subsp. nov., respectively. The type strains of the subspecies are subsp. piercei ICPB 50025 (=ATTC 35879 T and ICMP 15197), subsp. multiplex ICPB 50039 (= ATTC 35871 and ICMP 15199), and subsp. pauca ICPB 50031 (= ICMP 15198).

Norman W Schaad - One of the best experts on this subject based on the ideXlab platform.

  • xylella fastidiosa subspecies x fastidiosa subsp correction fastidiosa correction subsp nov x fastidiosa subsp multiplex subsp nov and x fastidiosa subsp pauca subsp nov
    Systematic and Applied Microbiology, 2004
    Co-Authors: Norman W Schaad, Elena Postnikova, George H Lacy, Mbarek Fatmi, Chungjan Chang
    Abstract:

    Xylella fastidiosa, a fastidious bacterium causing disease in over 100 plant species, is classified as a single species, although genetic studies support multiple Taxons. To determine the Taxonomic relatedness among strains of X. fastidiosa, we conducted DNA-DNA relatedness assays and sequenced the 16S-23S intergenic spacer (ITS) region using 26 strains from 10 hosts. Under stringent conditions (Tm -15 degrees C), the DNA relatedness for most X. fastidiosa strains was *70%. However, at high stringency (Tm -8 degrees C), three distinct genotypes (A, B, and C) were revealed. Taxon A included strains from cultivated grape, alfalfa, almond (two), and maple, interrelated by 85% (mean); Taxon B included strains from peach, elm, plum, pigeon grape, sycamore, and almond (one), interrelated by 84%; and Taxon C included only strains from citrus, interrelated by 87%. The mean reciprocal relatedness between Taxons A and B, A and C, and B and C, were 58, 41, and 45%, respectively. ITS results also indicated the same grouping; Taxons A and B, A and C, and B and C had identities of 98.7, 97.9, and 99.2%, respectively. Previous and present phenotypic data supports the molecular data. Taxon A strains grow faster on Pierce's disease agar medium whereas B and C strains grow more slowly. Taxon B and C strains are susceptible to penicillin and resistant to carbenicillin whereas A strains are opposite. Each Taxon can be differentiated serologically as well as by structural proteins. We propose Taxons A, B, and C be named X. fastidiosa subsp. fastidiosa [correction] subsp. nov, subsp. multiplex, subsp. nov., and subsp. pauca, subsp. nov., respectively. The type strains of the subspecies are subsp. fastidiosa [correction] ICPB 50025 (= ATTC 35879T and ICMP 15197), subsp. multiplex ICPB 50039 (= ATTC 35871 and ICMP 15199), and subsp. pauca ICPB 50031 (= ICMP 15198).

  • xylella fastidiosa subspecies x fastidiosa subsp piercei subsp nov x fastidiosa subsp multiplex subsp nov and x fastidiosa subsp pauca subsp nov
    Systematic and Applied Microbiology, 2004
    Co-Authors: Norman W Schaad, Elena Postnikova, George H Lacy, Mbarek Fatmi, Chungjan Chang
    Abstract:

    Summary Xylella fastidiosa, a fastidious bacterium causing disease in over 100 plant species, is classified as a single species, although genetic studies support multiple Taxons. To determine the Taxonomic relatedness among strains of X. fastidiosa, we conducted DNA-DNA relatedness assays and sequenced the 16S‐23S intergenic spacer (ITS) region using 26 strains from 10 hosts. Under stringent conditions (Tm ‐15 °C), the DNA relatedness for most X. fastidiosa strains was ≥70%. However, at high stringency (Tm ‐8 °C), three distinct genotypes (A, B, and C) were revealed. Taxon A included strains from cultivated grape, alfalfa, almond (two), and maple, interrelated by 85% (mean); Taxon B included strains from peach, elm, plum, pigeon grape, sycamore, and almond (one), interrelated by 84%; and Taxon C included only strains from citrus, interrelated by 87%. The mean reciprocal relatedness between Taxons A and B, A and C, and B and C, were 58, 41, and 45%, respectively. ITS results also indicated the same grouping ; Taxons A and B, A and C, and B and C had identities of 98.7, 97.9, and 99.2%, respectively. Previous and present phenotypic data supports the molecular data. Taxon A strains grow faster on Pierce’s disease agar medium whereas B and C strains grow more slowly. Taxon B and C strains are susceptible to penicillin and resistant to carbenicillin whereas A strains are opposite. Each Taxon can be differentiated serologically as well as by structural proteins. We propose Taxons A, B, and C be named X. fastidiosa subsp. piercei, subsp. nov, subsp. multiplex, subsp. nov., and subsp. pauca, subsp. nov., respectively. The type strains of the subspecies are subsp. piercei ICPB 50025 (=ATTC 35879 T and ICMP 15197), subsp. multiplex ICPB 50039 (= ATTC 35871 and ICMP 15199), and subsp. pauca ICPB 50031 (= ICMP 15198).

Elena Postnikova - One of the best experts on this subject based on the ideXlab platform.

  • xylella fastidiosa subspecies x fastidiosa subsp correction fastidiosa correction subsp nov x fastidiosa subsp multiplex subsp nov and x fastidiosa subsp pauca subsp nov
    Systematic and Applied Microbiology, 2004
    Co-Authors: Norman W Schaad, Elena Postnikova, George H Lacy, Mbarek Fatmi, Chungjan Chang
    Abstract:

    Xylella fastidiosa, a fastidious bacterium causing disease in over 100 plant species, is classified as a single species, although genetic studies support multiple Taxons. To determine the Taxonomic relatedness among strains of X. fastidiosa, we conducted DNA-DNA relatedness assays and sequenced the 16S-23S intergenic spacer (ITS) region using 26 strains from 10 hosts. Under stringent conditions (Tm -15 degrees C), the DNA relatedness for most X. fastidiosa strains was *70%. However, at high stringency (Tm -8 degrees C), three distinct genotypes (A, B, and C) were revealed. Taxon A included strains from cultivated grape, alfalfa, almond (two), and maple, interrelated by 85% (mean); Taxon B included strains from peach, elm, plum, pigeon grape, sycamore, and almond (one), interrelated by 84%; and Taxon C included only strains from citrus, interrelated by 87%. The mean reciprocal relatedness between Taxons A and B, A and C, and B and C, were 58, 41, and 45%, respectively. ITS results also indicated the same grouping; Taxons A and B, A and C, and B and C had identities of 98.7, 97.9, and 99.2%, respectively. Previous and present phenotypic data supports the molecular data. Taxon A strains grow faster on Pierce's disease agar medium whereas B and C strains grow more slowly. Taxon B and C strains are susceptible to penicillin and resistant to carbenicillin whereas A strains are opposite. Each Taxon can be differentiated serologically as well as by structural proteins. We propose Taxons A, B, and C be named X. fastidiosa subsp. fastidiosa [correction] subsp. nov, subsp. multiplex, subsp. nov., and subsp. pauca, subsp. nov., respectively. The type strains of the subspecies are subsp. fastidiosa [correction] ICPB 50025 (= ATTC 35879T and ICMP 15197), subsp. multiplex ICPB 50039 (= ATTC 35871 and ICMP 15199), and subsp. pauca ICPB 50031 (= ICMP 15198).

  • xylella fastidiosa subspecies x fastidiosa subsp piercei subsp nov x fastidiosa subsp multiplex subsp nov and x fastidiosa subsp pauca subsp nov
    Systematic and Applied Microbiology, 2004
    Co-Authors: Norman W Schaad, Elena Postnikova, George H Lacy, Mbarek Fatmi, Chungjan Chang
    Abstract:

    Summary Xylella fastidiosa, a fastidious bacterium causing disease in over 100 plant species, is classified as a single species, although genetic studies support multiple Taxons. To determine the Taxonomic relatedness among strains of X. fastidiosa, we conducted DNA-DNA relatedness assays and sequenced the 16S‐23S intergenic spacer (ITS) region using 26 strains from 10 hosts. Under stringent conditions (Tm ‐15 °C), the DNA relatedness for most X. fastidiosa strains was ≥70%. However, at high stringency (Tm ‐8 °C), three distinct genotypes (A, B, and C) were revealed. Taxon A included strains from cultivated grape, alfalfa, almond (two), and maple, interrelated by 85% (mean); Taxon B included strains from peach, elm, plum, pigeon grape, sycamore, and almond (one), interrelated by 84%; and Taxon C included only strains from citrus, interrelated by 87%. The mean reciprocal relatedness between Taxons A and B, A and C, and B and C, were 58, 41, and 45%, respectively. ITS results also indicated the same grouping ; Taxons A and B, A and C, and B and C had identities of 98.7, 97.9, and 99.2%, respectively. Previous and present phenotypic data supports the molecular data. Taxon A strains grow faster on Pierce’s disease agar medium whereas B and C strains grow more slowly. Taxon B and C strains are susceptible to penicillin and resistant to carbenicillin whereas A strains are opposite. Each Taxon can be differentiated serologically as well as by structural proteins. We propose Taxons A, B, and C be named X. fastidiosa subsp. piercei, subsp. nov, subsp. multiplex, subsp. nov., and subsp. pauca, subsp. nov., respectively. The type strains of the subspecies are subsp. piercei ICPB 50025 (=ATTC 35879 T and ICMP 15197), subsp. multiplex ICPB 50039 (= ATTC 35871 and ICMP 15199), and subsp. pauca ICPB 50031 (= ICMP 15198).

George H Lacy - One of the best experts on this subject based on the ideXlab platform.

  • xylella fastidiosa subspecies x fastidiosa subsp correction fastidiosa correction subsp nov x fastidiosa subsp multiplex subsp nov and x fastidiosa subsp pauca subsp nov
    Systematic and Applied Microbiology, 2004
    Co-Authors: Norman W Schaad, Elena Postnikova, George H Lacy, Mbarek Fatmi, Chungjan Chang
    Abstract:

    Xylella fastidiosa, a fastidious bacterium causing disease in over 100 plant species, is classified as a single species, although genetic studies support multiple Taxons. To determine the Taxonomic relatedness among strains of X. fastidiosa, we conducted DNA-DNA relatedness assays and sequenced the 16S-23S intergenic spacer (ITS) region using 26 strains from 10 hosts. Under stringent conditions (Tm -15 degrees C), the DNA relatedness for most X. fastidiosa strains was *70%. However, at high stringency (Tm -8 degrees C), three distinct genotypes (A, B, and C) were revealed. Taxon A included strains from cultivated grape, alfalfa, almond (two), and maple, interrelated by 85% (mean); Taxon B included strains from peach, elm, plum, pigeon grape, sycamore, and almond (one), interrelated by 84%; and Taxon C included only strains from citrus, interrelated by 87%. The mean reciprocal relatedness between Taxons A and B, A and C, and B and C, were 58, 41, and 45%, respectively. ITS results also indicated the same grouping; Taxons A and B, A and C, and B and C had identities of 98.7, 97.9, and 99.2%, respectively. Previous and present phenotypic data supports the molecular data. Taxon A strains grow faster on Pierce's disease agar medium whereas B and C strains grow more slowly. Taxon B and C strains are susceptible to penicillin and resistant to carbenicillin whereas A strains are opposite. Each Taxon can be differentiated serologically as well as by structural proteins. We propose Taxons A, B, and C be named X. fastidiosa subsp. fastidiosa [correction] subsp. nov, subsp. multiplex, subsp. nov., and subsp. pauca, subsp. nov., respectively. The type strains of the subspecies are subsp. fastidiosa [correction] ICPB 50025 (= ATTC 35879T and ICMP 15197), subsp. multiplex ICPB 50039 (= ATTC 35871 and ICMP 15199), and subsp. pauca ICPB 50031 (= ICMP 15198).

  • xylella fastidiosa subspecies x fastidiosa subsp piercei subsp nov x fastidiosa subsp multiplex subsp nov and x fastidiosa subsp pauca subsp nov
    Systematic and Applied Microbiology, 2004
    Co-Authors: Norman W Schaad, Elena Postnikova, George H Lacy, Mbarek Fatmi, Chungjan Chang
    Abstract:

    Summary Xylella fastidiosa, a fastidious bacterium causing disease in over 100 plant species, is classified as a single species, although genetic studies support multiple Taxons. To determine the Taxonomic relatedness among strains of X. fastidiosa, we conducted DNA-DNA relatedness assays and sequenced the 16S‐23S intergenic spacer (ITS) region using 26 strains from 10 hosts. Under stringent conditions (Tm ‐15 °C), the DNA relatedness for most X. fastidiosa strains was ≥70%. However, at high stringency (Tm ‐8 °C), three distinct genotypes (A, B, and C) were revealed. Taxon A included strains from cultivated grape, alfalfa, almond (two), and maple, interrelated by 85% (mean); Taxon B included strains from peach, elm, plum, pigeon grape, sycamore, and almond (one), interrelated by 84%; and Taxon C included only strains from citrus, interrelated by 87%. The mean reciprocal relatedness between Taxons A and B, A and C, and B and C, were 58, 41, and 45%, respectively. ITS results also indicated the same grouping ; Taxons A and B, A and C, and B and C had identities of 98.7, 97.9, and 99.2%, respectively. Previous and present phenotypic data supports the molecular data. Taxon A strains grow faster on Pierce’s disease agar medium whereas B and C strains grow more slowly. Taxon B and C strains are susceptible to penicillin and resistant to carbenicillin whereas A strains are opposite. Each Taxon can be differentiated serologically as well as by structural proteins. We propose Taxons A, B, and C be named X. fastidiosa subsp. piercei, subsp. nov, subsp. multiplex, subsp. nov., and subsp. pauca, subsp. nov., respectively. The type strains of the subspecies are subsp. piercei ICPB 50025 (=ATTC 35879 T and ICMP 15197), subsp. multiplex ICPB 50039 (= ATTC 35871 and ICMP 15199), and subsp. pauca ICPB 50031 (= ICMP 15198).

Mbarek Fatmi - One of the best experts on this subject based on the ideXlab platform.

  • xylella fastidiosa subspecies x fastidiosa subsp correction fastidiosa correction subsp nov x fastidiosa subsp multiplex subsp nov and x fastidiosa subsp pauca subsp nov
    Systematic and Applied Microbiology, 2004
    Co-Authors: Norman W Schaad, Elena Postnikova, George H Lacy, Mbarek Fatmi, Chungjan Chang
    Abstract:

    Xylella fastidiosa, a fastidious bacterium causing disease in over 100 plant species, is classified as a single species, although genetic studies support multiple Taxons. To determine the Taxonomic relatedness among strains of X. fastidiosa, we conducted DNA-DNA relatedness assays and sequenced the 16S-23S intergenic spacer (ITS) region using 26 strains from 10 hosts. Under stringent conditions (Tm -15 degrees C), the DNA relatedness for most X. fastidiosa strains was *70%. However, at high stringency (Tm -8 degrees C), three distinct genotypes (A, B, and C) were revealed. Taxon A included strains from cultivated grape, alfalfa, almond (two), and maple, interrelated by 85% (mean); Taxon B included strains from peach, elm, plum, pigeon grape, sycamore, and almond (one), interrelated by 84%; and Taxon C included only strains from citrus, interrelated by 87%. The mean reciprocal relatedness between Taxons A and B, A and C, and B and C, were 58, 41, and 45%, respectively. ITS results also indicated the same grouping; Taxons A and B, A and C, and B and C had identities of 98.7, 97.9, and 99.2%, respectively. Previous and present phenotypic data supports the molecular data. Taxon A strains grow faster on Pierce's disease agar medium whereas B and C strains grow more slowly. Taxon B and C strains are susceptible to penicillin and resistant to carbenicillin whereas A strains are opposite. Each Taxon can be differentiated serologically as well as by structural proteins. We propose Taxons A, B, and C be named X. fastidiosa subsp. fastidiosa [correction] subsp. nov, subsp. multiplex, subsp. nov., and subsp. pauca, subsp. nov., respectively. The type strains of the subspecies are subsp. fastidiosa [correction] ICPB 50025 (= ATTC 35879T and ICMP 15197), subsp. multiplex ICPB 50039 (= ATTC 35871 and ICMP 15199), and subsp. pauca ICPB 50031 (= ICMP 15198).

  • xylella fastidiosa subspecies x fastidiosa subsp piercei subsp nov x fastidiosa subsp multiplex subsp nov and x fastidiosa subsp pauca subsp nov
    Systematic and Applied Microbiology, 2004
    Co-Authors: Norman W Schaad, Elena Postnikova, George H Lacy, Mbarek Fatmi, Chungjan Chang
    Abstract:

    Summary Xylella fastidiosa, a fastidious bacterium causing disease in over 100 plant species, is classified as a single species, although genetic studies support multiple Taxons. To determine the Taxonomic relatedness among strains of X. fastidiosa, we conducted DNA-DNA relatedness assays and sequenced the 16S‐23S intergenic spacer (ITS) region using 26 strains from 10 hosts. Under stringent conditions (Tm ‐15 °C), the DNA relatedness for most X. fastidiosa strains was ≥70%. However, at high stringency (Tm ‐8 °C), three distinct genotypes (A, B, and C) were revealed. Taxon A included strains from cultivated grape, alfalfa, almond (two), and maple, interrelated by 85% (mean); Taxon B included strains from peach, elm, plum, pigeon grape, sycamore, and almond (one), interrelated by 84%; and Taxon C included only strains from citrus, interrelated by 87%. The mean reciprocal relatedness between Taxons A and B, A and C, and B and C, were 58, 41, and 45%, respectively. ITS results also indicated the same grouping ; Taxons A and B, A and C, and B and C had identities of 98.7, 97.9, and 99.2%, respectively. Previous and present phenotypic data supports the molecular data. Taxon A strains grow faster on Pierce’s disease agar medium whereas B and C strains grow more slowly. Taxon B and C strains are susceptible to penicillin and resistant to carbenicillin whereas A strains are opposite. Each Taxon can be differentiated serologically as well as by structural proteins. We propose Taxons A, B, and C be named X. fastidiosa subsp. piercei, subsp. nov, subsp. multiplex, subsp. nov., and subsp. pauca, subsp. nov., respectively. The type strains of the subspecies are subsp. piercei ICPB 50025 (=ATTC 35879 T and ICMP 15197), subsp. multiplex ICPB 50039 (= ATTC 35871 and ICMP 15199), and subsp. pauca ICPB 50031 (= ICMP 15198).