The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Yingjin Yuan - One of the best experts on this subject based on the ideXlab platform.
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metabolic profiling as a tool for understanding defense response of Taxus cuspidata cells to shear stress
Biotechnology Progress, 2009Co-Authors: Peipei Han, Yingjin YuanAbstract:To obtain a better understanding of responsive mechanism of plant cells in response to hydrodynamic mechanical stress, a metabolic profiling approach was used to profile metabolite changes of Taxus cuspidata cells under laminar shear stress. A total of 65 intracellular metabolites were identified and quantified, using gas chromatography coupled to time-of-flight mass spectrometry. Potential biomarkers were found by the principal component analysis as well as partial least squares combined with variable influence in the projection. Trehalose, sorbitol, ascorbate, sucrose, and gluconic acid were mainly responsible for the discrimination between shear stress induced cells and control cells. Further analysis by mapping measured metabolite concentrations onto the metabolic network revealed that shear stress imposed restrictions on primary metabolic pathways by inhibiting tricarboxylic acid cycle, glycolysis, and N metabolism. To adapt to the shear condition, cells responded by starting defensive programs. These defensive programs included coinduction of glycolysis and sucrose metabolism, accumulation of compatible solutes, and antioxidative strategy. A strategy of defense mechanisms at the level of metabolites for T. cuspidata cells when challenged with the shear stress was proposed.
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cerium elicitor induced phosphatidic acid triggers apoptotic signaling development in Taxus cuspidata cell suspension cultures
Chemistry and Physics of Lipids, 2009Co-Authors: Song Yang, Yingjin YuanAbstract:Abstract Degradation of membrane phospholipids is associated with apoptotic responses, but the signaling development of this degradation is not well understood. Cerium (Ce4+), an important rare earth element, induces cellular apoptosis and taxol biosynthesis in Taxus cuspidata suspension cultures. Here, using mass spectrometry and biochemical technique, we demonstrated that the phospholipase D (PLD) was rapidly activated by Ce4+ and hydrolyzed structural phospholipids to generate lipid signal molecule, phosphatidic acid (PA). 1-Butanol, an antagonist of PLD-dependent PA production, blocked the biphasic burst of superoxide anions (O2 −) and thus mitigated cellular apoptosis. The time-course analysis of PA accumulation and ERK-like mitogen-activated protein kinase (MAPK) regulation indicated PA generation preceded MAPK activation, suggesting that the rapid accumulation of PA might be required for the initial MAPK activity. After 2 h of Ce4+ elicitation, however, PA-induced O2 − burst, forming a negative regulation to MAPK activity, which in turn led to apoptotic signaling development.
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spatial temporal distribution of nitric oxide involved in regulation of phenylalanine ammonialyase activation and taxol production in immobilized Taxus cuspidata cells
Journal of Biotechnology, 2009Co-Authors: Wenhai Xiao, Jingsheng Cheng, Yingjin YuanAbstract:Abstract The generation of nitric oxide (NO) in Taxus cuspidata in immobilized support matrices and the potential role of NO as signal molecular in regulation of Taxol production were investigated. It was found that the immobilization induced a spatial and temporal-dependent NO burst in immobilized supported matrices. NO level reached the maximum in the central zone of immobilized supported matrices on day 20, which was more than twice compared with that in suspended cells. Further investigations showed that the phenylalanine ammonialyase (PAL) activity and Taxol production of the 20-day-old immobilized T. cuspidata cells increased by onefold and 11% after 4 h treatment with 20 μM NO donor (sodium nitroprusside), respectively. NO inhibitor N ω -nitro- l -arginine and NO scavenger 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxyde partially blocked PAL activity and Taxol accumulation in immobilized cells. These results suggest that NO plays a signal role in regulation of PAL activity and Taxol production in immobilized T. cuspidata cells.
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lipidomic analysis reveals activation of phospholipid signaling in mechanotransduction of Taxus cuspidata cells in response to shear stress
The FASEB Journal, 2009Co-Authors: Peipei Han, Yingjin YuanAbstract:Lipid signaling involved in mechanotransduction processes in response to shear stress in plants remained elusive. To understand the responses of phospholipids in shear stress-induced mechanotransduction, a lipidomic approach was employed to profile phospholipid species of Taxus cuspidata cells under laminar shear stress. A total of 99 phospholipid species were profiled quantitatively, using the LC/ESI/MS(n) procedure. Potential biomarkers were found by the principal component analysis (PCA) as well as partial least squares (PLS) combined with variable influence in the projection (VIP). Phosphatidic acid (PA) and lysophosphatidylcholine (LysoPC) were two important lipid groups that were responsible for the discrimination between shear stress induced and control cells. Further research revealed that shear stress enhanced the activation of phospholipase D (PLD) and phospholipase C (PLC) compared with control cells and consequently increased PA content in shear stress induced T. cuspidata cells. These results demonstrate that phospholipids and related phospholipases play important roles in mechanotransduction of T. cuspidata cells in response to shear stress.
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Responses of Taxus cuspidata to hydrodynamics in bubble column bioreactors with different sparging nozzle sizes.
Biochemical Engineering Journal, 2009Co-Authors: Cheng Zhong, Yingjin YuanAbstract:Abstract Studying bioreactor hydrodynamics under growth conditions holds great significance in the scale-up of plant cell cultures. In this study, a 3D Laser Doppler Anemometer (LDA) system was used to measure liquid velocity fields in bubble columns with different nozzle sizes, and their hydrodynamic stresses were calculated by liquid velocity fluctuation components. The magnitude of axial normal Reynolds stress was higher compared to other directions, varying from 2.0 Pa to 7.2 Pa. The maximum axial normal stress took place near the gas–liquid interface, followed by the sparger where bubbles form and the bulk flow area where bubbles rise, suggesting from a hydrodynamic perspective that cell damage occurs mainly at the gas–liquid interface. Reynolds stresses at the gas–liquid interface increased with decreasing nozzle size of distributor. Taxus cuspidata cultured in bubble columns with larger nozzle sizes showed better performance with respect to cell growth and viability, which matched well with hydrodynamics fluctuation in bubble columns with different nozzle sizes. The correlation of cellular response with hydrodynamics may be helpful in optimizing bioreactor design and scale-up of plant cell culture.
Frank Dicosmo - One of the best experts on this subject based on the ideXlab platform.
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efficient paclitaxel production using protoplasts isolated from cultured cells of Taxus cuspidata
Planta Medica, 2002Co-Authors: Hideki Aoyagi, Frank Dicosmo, Hideo TanakaAbstract:Efficient isolation of protoplasts from Taxus cuspidata cultured cells, localization of paclitaxel in the cultured cells, and efficient production of paclitaxel by protoplasts were studied. Hemicellulase, potassium citric acid solution, and degassing treatments were effective in increasing the yield of protoplasts isolated from T. cuspidata cultured cells. Protoplasts yields (3.2 - 6.4 x 10(6) number/g-fresh weight cells) were achieved by combining the various treatments with specific culture and cell phases. It was found that about 30% and 35% of paclitaxel in the cells was located in cell walls and/or between the cell wall and cell membrane (CW) of suspension cells in the growth phase and in the stationary phase, respectively. About 30% and 43% of paclitaxel in the cells was located in CW of the cells grown in solid culture in growth phase and in the stationary phase, respectively. In comparison with cell suspension culture, protoplasts in a static culture and the protoplasts immobilized in agarose gel in shaking culture resulted to about 6 times increase in the extracellular paclitaxel accumulation.
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an abietane from Taxus cuspidata cell suspension cultures
ChemInform, 1999Co-Authors: Kristina Salciccioli, Frank Dicosmo, William F ReynoldsAbstract:Abstract An abietane diterpenoid was isolated from an ethyl acetate extraction of Taxus cuspidata cell suspension media. The structural assignments were established by mass spectroscopy, and 1 H and 13 C NMR studies including COSY, HMQC and HMBC. This compound was determined to be 3,20-epoxy-12-methoxy-8,11,13-abietatriene-3,7,11-triol.
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Acetyl CoA:10-deacetylbaccatin-III-10-O-acetyltransferase activity in leaves and cell suspension cultures of Taxus cuspidata
Phytochemistry, 1998Co-Authors: Jason J. Pennington, Sherwin A. Nicholson, David G I Kingston, Arthur Germano Fett-neto, Frank DicosmoAbstract:Abstract Partially purified acetyl CoA:10-deacetylbaccatin-III-10-orthoxy-acetyltransferase from leaves and 3-year-old cell suspension cultures of Taxus cuspidata yields baccatin-III from 10-deacetyl-baccatin-III in the presence of acetyl-CoA. The enzyme is substrate selective and does not significantly catalyze the conversion of 10-deacetyltaxol to paclitaxel (Taxol®). Ammonium sulfate precipitations and anion exchange column chromatography yielded partially purified enzyme from Taxus cuspidata leaves and cell suspensions. The 0–40% ammonium sulfate-precipitated protein fraction showed consistent and significant enzyme activity. Cell culture-protein extracts yielded higher activities of the enzyme than did leaf-protein extracts. 1 ml aliquots of enzyme preparation with 150 nmol of 10-deacetylbaccatin-III and 4.4 nmol of [1- 14 C]acetyl CoA for 1 h at 30°C produce approximately 1.1 pmol baccatin-III per hour per mg of protein. The products of the reactions were eluted using photodiode array HPLC and fractions containing baccatin-III or paclitaxel were collected and scintillation counted to determine the amount of radiolabeled product formed. Identity of product peaks was confirmed by retention time, photodiode array UV spectrophotometry and co-chromatography with authentic standards.
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improved taxol yield by aromatic carboxylic acid and amino acid feeding to cell cultures of Taxus cuspidata
Biotechnology and Bioengineering, 1994Co-Authors: Arthur Germano Fettneto, Jason J. Pennington, Sherwin A. Nicholson, Stewart J Melanson, Frank DicosmoAbstract:Cell culture of Taxus cuspidata represents an alternative to whole plant extraction as a source of taxol and related taxanes. Feeding phenylalanine to callus cultures was previously shown to result in increased taxol yields, probably due to the involvement of this amino acid as a precursor for the N-benzoylphenylisoserine side chain of taxol. Inthis study, we have examined the effect of various concentrations of phenylalanine, benzoic acid, N-benzoylglycine, serine, glycine, alanine, and 3-amino-3-phenyl-propionic acid on taxol accumulation in 2-year-old cell suspensions of Taxus cuspidata, cell line FCL1F, and in developing callus cultures of T. cuspidata. All compounds tested were included in media at stationary phase (suspensions) or after the period of fastest growth (calli). Alanine and 3-amino-3-phenyl-propionicacid were tested only in callus cultures and did not affect taxol accumulation. Significant increases or trends toward increases in taxol accumulationin callus and suspensions were observed in the presence of phenylalanine, benzoic acid, N-benzoylglycine, serine, and glycine. The greatest increases in taxol accumulation were observed in the presence of various concentrations of phenylalanine (1 mM for callus; 0.05, 0.1, and 0.2 mM for suspensions) and benzoic acid (0.2 and 1 mM for callus and 0.05, 0.1, and 0.2 mM for suspensions). Increases in taxol yields of cell suspensions in the presence of the most effective precursors brought taxol amounts at stationary phase from 2 mug . g(-1) to approximately 10 mug . g(-1) of the extracted dry weight. The results are discussed in termsof possible implications to taxol biosynthesis and in terms of practical applications to large-scale cell culture systems for the production ofthis drug. (c) 1994 John Wiley & Sons, Inc.
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kinetics of taxol production growth and nutrient uptake in cell suspensions of Taxus cuspidata
Biotechnology and Bioengineering, 1994Co-Authors: Arthur Germano Fettneto, Wen Yi Zhang, Frank DicosmoAbstract:Cell culture of Taxus cuspidata may represent an alternative to extraction of bark as a source of taxol and related taxanes. Cell suspensions of a cell line of T. cuspidata were grown for 44 days in shake flasks containing B5C2 medium. Throughout the growth cycle, fresh and dry weight accumulation, taxol yield on a dry weight basis, taxol accumulation in the medium, pH and pigmentation variation in the medium, as well as the uptake of sucrose, glucose, fructose, nitrate, and inorganic phosphate from the culture medium were examined. The results showed that the growth was relatively slow (doubling times of 17 and 20 days for fresh and dry weight, respectively), and taxol accumulation in the cells was non-growth related (higher in the stationary phase) and at relatively low levels (up to 4 mug/g of the extracted dry weight). Taxol concentration in the medium had two peaks: one during the early (0.4mug/mL) and another during the late (0.1-mug/mL) parts of the growth cycle. On a volumetric basis, the average total amount of taxol produced during the stationary phase (day 38) was 0.15 mug/mL, of which approximately 66% was in the medium and 34% was in the cells. Total carbohydrate uptake was closely associated with the increase in dry biomass. Sucrose was apparently extracellularly hydrolyzed after the first 6 days of culture; glucose was used before fructose. Nitrate was assimilated throughout the growth cycle, but phosphate was absorbed within the first week of culture. The pH variation showed an initial drop followed by a trend toward alkalinization for most of the growth period. Dark pigmentation in the medium increased progressively, particularly during the stationary phase. (c) 1994 John Wiley & Sons, Inc.
Hiromasa Kiyota - One of the best experts on this subject based on the ideXlab platform.
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new 6 8 6 taxanes isolated from the heartwood of Taxus cuspidata
Helvetica Chimica Acta, 2012Co-Authors: Kai Zhang, Hiromasa Kiyota, Qing-wen Shi, Chang Hong Huo, Manli Zhang, Francoise Sauriol, Yufang Wang, Mei DongAbstract:Two new natural taxanes were isolated from the heartwood of Taxus cuspidata. The structures were established as rel-(2α,5α,7β,9α,10β,12α)-7,9-bis(acetyloxy)-2-(benzoyloxy)-11,12-epoxy-1,5-dihydroxy-10-[(hydroxyacetyl)oxy]tax-4(20)-en-13-one (1), and (2α,5α,10β,14β)-taxa-4(20),11-diene-2,5,10,14-tetrol 2-acetate (2) on the basis of spectroscopic analysis.
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a novel taxane 13 glucoside and other taxanes from the leaves of Taxus cuspidata
Planta Medica, 2011Co-Authors: Mei Dong, Qing-wen Shi, Chang Hong Huo, Manli Zhang, Francoise Sauriol, Yufang Wang, Bin Cong, Hiromasa KiyotaAbstract:Three new taxanes, 2 α,9 α,10 β-triacetoxy-13 α-( β- D-glucopyranosyloxy)taxa-4(20),11-dien-5 α-ol ( 1), 5 α,10 β,13 α-triacetoxytax-11-ene-2 α,7 β,9 α,20-tetraol ( 2), and 5 α,10 β,13 β-triacetoxy-2 α,7 β-dihydroxy-2(3 → 20) ABEOtaxa-4(20),11-dien-9-one ( 3), were isolated from the leaves of the Japanese yew, Taxus cuspidata. Compound 1 is the first example of a taxane with 13-glycosidic linkage.
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a taxane with a novel 9α 13α oxygen bridge from Taxus cuspidata needles
Tetrahedron Letters, 2008Co-Authors: Manli Zhang, Qing-wen Shi, Mei Dong, Chang Hong Huo, Yufang Wang, Bin Cong, Hiromasa KiyotaAbstract:Abstract A novel taxane with an unprecedented hemiacetal ring between C-13 and C-9 was isolated from the needles of Taxus cuspidata . The structure was characterized as (12αH)-2α,10β-diacetoxy-5α-cinnamoyloxy-9α,13α-epoxytax-4(20)-ene-11β,13β-diol ( 1 ). This is the first example of a natural taxane with a C-13 and C-9 oxygen bridge to form an unusual 6/8/6/6-membered ring system.
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structural determination of a new 2 3 20 abeotaxane with an unusual 13β substitution pattern and a new 6 8 6 ring taxane from Taxus cuspidata
Magnetic Resonance in Chemistry, 2007Co-Authors: Chang Hong Huo, Qing-wen Shi, Yufang Wang, Xiping Zhang, Hiromasa KiyotaAbstract:A new 2(3 --> 20)abeotaxane with an unusual 13beta-substitution pattern and a new 6/8/6-ring taxane were isolated from the methanol extract of the needles of Taxus cuspidata. The structures were established as 2alpha,7beta-diacetoxy-5alpha,10beta,13beta-trihydroxy-2(3 --> 20)abeotaxa-4(20), 11-dien-9-one (1) and 2alpha,5alpha,7beta,9alpha,13alpha-pentahydroxy-10beta-acetoxytaxa-4(20),11-diene (2) on the basis of 1D and 2D NMR spectral data and high-resolution FAB-MS analyses.
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7 8beta dihydroponasterone a a new phytoecdysteroid from the needles of the japanese yew Taxus cuspidata
Journal of the Brazilian Chemical Society, 2007Co-Authors: Qing-wen Shi, Mei Dong, Chang Hong Huo, Teiko Yamada, Hiromasa KiyotaAbstract:A new plant ecdysteroid 7,8b-dihydroponasterone A, together with ponasterone A, were isolated from the methanol extract of the needles of the Japanese yew, Taxus cuspidata. Their structures were elucidated on the basis of spectroscopic analysis including 1H NMR, 13C NMR, 1H-1H COSY, NOESY, HMQC and HMBC and confirmed by high-resolution FABMS data.
Jun'ichi Kobayashi - One of the best experts on this subject based on the ideXlab platform.
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taxezopidines o and p new taxoids from Taxus cuspidata
Heterocycles, 2007Co-Authors: Jun'ichi Kobayashi, Haruaki Ishiyama, Yuka Kakuguchi, Eri AritaAbstract:Two new taxoids, taxezopidines O (1) and P (2), have been isolated from seeds of the Japanese yew Taxus cuspidata, and the structures and relative stereochemistry were elucidated on the basis of spectroscopic data. The absolute stereochemistry of a 3-N-methylamino-3-phenylpropanoyl group in 1 was determined by HPLC analysis for FDAA (1-fluoro-2,4-dinitrophenyl-5-L-alanine amide) derivative of hydrolysate of 1.
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taxezopidines m and n taxoids from the japanese yew Taxus cuspidata
Journal of Natural Products, 2005Co-Authors: Hiroshi Morita, Izumi Machida, Yusuke Hirasawa, Jun'ichi KobayashiAbstract:Two new taxoids, taxezopidines M (1) and N (2), have been isolated from seeds of the Japanese yew, Taxus cuspidata, and new structures were elucidated on the basis of spectroscopic data. The absolute configuration of a 3-N,N-(dimethylamino)-3-phenylpropanoyl group in 1 and 2 was determined to be R in each case by chiral HPLC analysis. The effect of 1 and 2 on the CaCl(2)-induced depolymerization of microtubules was investigated.
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Biological activity and chemistry of taxoids from the Japanese yew, Taxus cuspidata.
Journal of natural products, 2004Co-Authors: Hideyuki Shigemori, Jun'ichi KobayashiAbstract:Approximately 120 taxoids have been isolated to date from the Japanese yew, Taxus cuspidata. These taxoids possess various skeletons containing 5/7/6-, 6/10/6-, 6/5/5/6-, 6/8/6-, or 6/12-membered r...
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Bioactive taxoids from the Japanese yew Taxus cuspidata
Medicinal research reviews, 2002Co-Authors: Jun'ichi Kobayashi, Hideyuki ShigemoriAbstract:A series of new taxoids, named Taxuspines A-H and J-Z (1-25) and taxezopidines A-H and J-L (26-36), have been isolated together with 37 known taxoids (37-73) including paclitaxel (53) from the Japanese yew, Taxus cuspidata Sieb. et Zucc. (Taxaceae). These new taxoids possess various skeletons containing 5/7/6, 6/10/6, 6/5/5/6, 6/8/6, or 6/12-membered ring systems. Among the new taxoids, some non-taxol-type compounds remarkably reduced CaCl(2)-induced depolymerization of microtubules, or increased cellular accumulation of vincristine in multidrug-resistant tumor cells as potent as verapamil. On the other hand, chemical derivatization of taxinine (37), one of major taxoids obtained from this yew, led to the discovery of unusual reactions of taxinine derivatives. Here we describe our recent results on the isolation, structure elucidation, and bioactivity of these new and known taxoids and the formation of unexpected products of the unusual reactions of taxinine.
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multidrug resistance reversal activity of taxoids from Taxus cuspidata in kb c2 and 2780ad cells
Japanese Journal of Cancer Research, 2000Co-Authors: Jun'ichi Kobayashi, Hideyuki Shigemori, Hirokazu Hosoyama, Mikihiko Naito, Zhesheng Chen, Shinichi Akiyama, Takashi TsuruoAbstract:Some non-taxol-type taxoids having neither an oxetane ring at C-4 and C-5 nor an N-acylphenyl-isoserine group at C-13, such as Taxuspine C, 2'-desacetoxyaustrospicatine, and 2-desacetoxytaxinine J, which were isolated from the Japanese yew Taxus cuspidata, increased cellular accu-mulation of vincristine (VCR) in multidrug-resistant 2780AD cells as potently as verapamil, and efficiently inhibited [(3)H]azidopine photolabeling of P-glycoprotein (P-gp). Taxuspine C, 2'-desacetoxyaustrospicatine, and 2-desacetoxytaxinine J at 10 microM completely reversed the resistance to colchicine, VCR, and taxol in KB-C2 cells, which overexpress P-gp, while taxinine and taxinine M showed no effect. Taxuspine C, 2'-desacetoxyaustrospicatine, and 2-desacetoxytaxinine J may be candidate pharmaceuticals for reversing multidrug resistance (MDR) and also may be good modifiers of MDR in cancer chemotherapy.
Qing-wen Shi - One of the best experts on this subject based on the ideXlab platform.
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two new non taxoids from leaves of Taxus cuspidata
Chemistry of Natural Compounds, 2014Co-Authors: Ruixia Guo, Qing-wen Shi, Mei Dong, Tiankun Ren, Manli Zhang, Francoise Sauriol, Chang Hong HuoAbstract:Two new non-taxoids, named (Z)-2-(2,4-dihydroxy-2,6,6-trimethylcyclohexylidene)acetic acid (1) and (E)-3-(4-hydroxy-3-methoxyphenyl)acrylic anhydride (2), were isolated from the leaves of Taxus cuspidata, and the structures were elucidated on the basis of one- and two-dimensional NMR (including 1H, 13C NMR, DEPT, 1H–1H COSY, HSQC, HMBC, and NOESY).
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new 6 8 6 taxanes isolated from the heartwood of Taxus cuspidata
Helvetica Chimica Acta, 2012Co-Authors: Kai Zhang, Hiromasa Kiyota, Qing-wen Shi, Chang Hong Huo, Manli Zhang, Francoise Sauriol, Yufang Wang, Mei DongAbstract:Two new natural taxanes were isolated from the heartwood of Taxus cuspidata. The structures were established as rel-(2α,5α,7β,9α,10β,12α)-7,9-bis(acetyloxy)-2-(benzoyloxy)-11,12-epoxy-1,5-dihydroxy-10-[(hydroxyacetyl)oxy]tax-4(20)-en-13-one (1), and (2α,5α,10β,14β)-taxa-4(20),11-diene-2,5,10,14-tetrol 2-acetate (2) on the basis of spectroscopic analysis.
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a novel taxane 13 glucoside and other taxanes from the leaves of Taxus cuspidata
Planta Medica, 2011Co-Authors: Mei Dong, Qing-wen Shi, Chang Hong Huo, Manli Zhang, Francoise Sauriol, Yufang Wang, Bin Cong, Hiromasa KiyotaAbstract:Three new taxanes, 2 α,9 α,10 β-triacetoxy-13 α-( β- D-glucopyranosyloxy)taxa-4(20),11-dien-5 α-ol ( 1), 5 α,10 β,13 α-triacetoxytax-11-ene-2 α,7 β,9 α,20-tetraol ( 2), and 5 α,10 β,13 β-triacetoxy-2 α,7 β-dihydroxy-2(3 → 20) ABEOtaxa-4(20),11-dien-9-one ( 3), were isolated from the leaves of the Japanese yew, Taxus cuspidata. Compound 1 is the first example of a taxane with 13-glycosidic linkage.
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a new taxane from the hard wood of Taxus cuspidata
ChemInform, 2010Co-Authors: Manli Zhang, Chang Hong Huo, Yufang Wang, Qing-wen ShiAbstract:structure elucidation of new 5,13-diacetyltaxinine M-13-enol (I), a new taxoid metabolite with an unusual double bond between C-13 and C-14
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taxanes from the leaves of Taxus cuspidata
Chemistry of Natural Compounds, 2010Co-Authors: Manli Zhang, Qing-wen Shi, Mei Dong, Chang Hong Huo, Jing Zhang, Shaoxia Zhang, Bin CongAbstract:A new taxane, 13-oxobaccatin III (2), and five known taxanes, 10-deacetyl-13-oxobaccatin III (1), baccatin III (3), 7-epi-10-deacetyltaxol (4), 19-debenzoyl-19-acetyltaxinine M (5), and 5a-decinnamoyltaxagifine (6), were isolated from the leaves of the Japanese yew, Taxus cuspidate. Their structures were established on the basis of the analysis of their spectral data.
