The Experts below are selected from a list of 10122 Experts worldwide ranked by ideXlab platform
Toyofumi Yamaguchi - One of the best experts on this subject based on the ideXlab platform.
-
3 azido 2 3 dideoxynucleoside 5 triphosphates inhibit telomerase activity in vitro and the corresponding nucleosides cause Telomere Shortening in human hl60 cells
Nucleic Acids Research, 2007Co-Authors: Xiaohong Liu, Tsukasa Ogawara, Mineo Saneyoshi, Hazuki Takahashi, Yoko Harada, Yuta Ogimura, Yoshiyuku Mizushina, Toyofumi YamaguchiAbstract:Telomerase adds telomeric DNA repeats to the ends of linear chromosomal DNA. 3′-Azido-3′-deoxythymidine 5′-triphosphate (AZTTP) is a known telomerase inhibitor. To obtain more selective and potent inhibitors that can be employed as tools for studying telomerase, we investigated the telomerase-inhibitory effects of purine nucleosides bearing a 3′-down azido group: 3′-azido-2′,3′-dideoxyguanosine (AZddG) 5′-triphosphate (AZddGTP), 3′-azido-2′,3′-dideoxy-6-thioguanosine (AZddSG) 5′-triphosphate (AZddSGTP), 3′-azido-2′,3′-dideoxyadenosine (AZddA) 5′-triphosphate (AZddATP) and 3′-azido-2′,3′-dideoxy-2-aminoadenosine (AZddAA) 5′-triphosphate (AZddAATP). Of these, AZddGTP showed the most potent inhibitory activity against HeLa cell telomerase. AZddGTP was significantly incorporated into the 3′-terminus of DNA by partially purified telomerase. However, AZddGTP did not exhibit significant inhibitory activity against DNA polymerases α and δ, suggesting that AZddGTP is a selective inhibitor of telomerase. We also investigated whether long-term treatment with these nucleosides could alter Telomere length and growth rates of human HL60 cells in culture. Southern hybridization analysis of genomic DNA prepared from cells cultured in the presence of AZddG and AZddAA revealed reproducible Telomere Shortening.
-
telomerase inhibition by 3 azido 2 3 dideoxynucleoside 5 triphosphates and Telomere Shortening in human cultured cells by the corresponding nucleosides
Nucleic acids symposium series (2004), 2006Co-Authors: Toyofumi Yamaguchi, Xiaohong Liu, Tsukasa Ogawara, Motoko Inomata, Mineo SaneyoshiAbstract:Telomerase is believed to be a good target for the development of antitumor agents. In this study, 3'-azido-2',3'-dideoxy-2-aminoadenosine (AZddAA), 3'-azido-2',3'-dideoxyadenosine (AZddA), 9-(3-azido-2,3-dideoxy-beta-D-ribofuranosyl)-2-aminopurine (AZddAP), 3'-azido-2-chloro-2',3'-dideoxyadenosine (AZddClA) and their triphosphate derivatives were synthesized. Telomerase assay studies showed that the 2-amino group plays an important role in the inhibitory activity of these compounds. In addition, AZddAA was found to cause Telomere Shortening in of HL60 cells in culture.
Mary Dozier - One of the best experts on this subject based on the ideXlab platform.
-
parental responsiveness moderates the association between early life stress and reduced Telomere length
Development and Psychopathology, 2013Co-Authors: Arun Asok, Kristin Bernard, Tania L Roth, Jeffrey B Rosen, Mary DozierAbstract:Early-life stress, such as maltreatment, institutionalization, and exposure to violence, is associated with accelerated Telomere Shortening. Telomere Shortening may thus represent a biomarker of early adversity. Previous studies have suggested that responsive parenting may protect children from the negative biological and behavioral consequences of early adversity. This study examined the role of parental responsiveness in buffering children from Telomere Shortening following experiences of early-life stress. We found that high-risk children had significantly shorter Telomeres than low-risk children, controlling for household income, birth weight, gender, and minority status. Further, parental responsiveness moderated the association between risk and Telomere length, with more responsive parenting associated with longer Telomeres only among high-risk children. These findings suggest that responsive parenting may have protective benefits on Telomere Shortening for young children exposed to early-life stress. Accordingly, this study has important implications for early parenting interventions.
-
parental responsiveness moderates the association between early life stress and reduced Telomere length
Development and Psychopathology, 2013Co-Authors: Arun Asok, Kristin Bernard, Tania L Roth, Jeffrey B Rosen, Mary DozierAbstract:Early-life stress, such as maltreatment, institutionalization, and exposure to violence, is associated with accelerated Telomere Shortening. Telomere Shortening may thus represent a biomarker of early adversity. Previous studies have suggested that responsive parenting may protect children from the negative biological and behavioral consequences of early adversity. This study examined the role of parental responsiveness in buffering children from Telomere Shortening following experiences of early-life stress. We found that high-risk children had significantly shorter Telomeres than low-risk children, controlling for household income, birth weight, gender, and minority status. Further, parental responsiveness moderated the association between risk and Telomere length, with more responsive parenting associated with longer Telomeres only among high-risk children. These findings suggest that responsive parenting may have protective benefits on Telomere Shortening for young children exposed to early-life stress. Therefore, this study has important implications for early parenting interventions.
Xiaohong Liu - One of the best experts on this subject based on the ideXlab platform.
-
3 azido 2 3 dideoxynucleoside 5 triphosphates inhibit telomerase activity in vitro and the corresponding nucleosides cause Telomere Shortening in human hl60 cells
Nucleic Acids Research, 2007Co-Authors: Xiaohong Liu, Tsukasa Ogawara, Mineo Saneyoshi, Hazuki Takahashi, Yoko Harada, Yuta Ogimura, Yoshiyuku Mizushina, Toyofumi YamaguchiAbstract:Telomerase adds telomeric DNA repeats to the ends of linear chromosomal DNA. 3′-Azido-3′-deoxythymidine 5′-triphosphate (AZTTP) is a known telomerase inhibitor. To obtain more selective and potent inhibitors that can be employed as tools for studying telomerase, we investigated the telomerase-inhibitory effects of purine nucleosides bearing a 3′-down azido group: 3′-azido-2′,3′-dideoxyguanosine (AZddG) 5′-triphosphate (AZddGTP), 3′-azido-2′,3′-dideoxy-6-thioguanosine (AZddSG) 5′-triphosphate (AZddSGTP), 3′-azido-2′,3′-dideoxyadenosine (AZddA) 5′-triphosphate (AZddATP) and 3′-azido-2′,3′-dideoxy-2-aminoadenosine (AZddAA) 5′-triphosphate (AZddAATP). Of these, AZddGTP showed the most potent inhibitory activity against HeLa cell telomerase. AZddGTP was significantly incorporated into the 3′-terminus of DNA by partially purified telomerase. However, AZddGTP did not exhibit significant inhibitory activity against DNA polymerases α and δ, suggesting that AZddGTP is a selective inhibitor of telomerase. We also investigated whether long-term treatment with these nucleosides could alter Telomere length and growth rates of human HL60 cells in culture. Southern hybridization analysis of genomic DNA prepared from cells cultured in the presence of AZddG and AZddAA revealed reproducible Telomere Shortening.
-
telomerase inhibition by 3 azido 2 3 dideoxynucleoside 5 triphosphates and Telomere Shortening in human cultured cells by the corresponding nucleosides
Nucleic acids symposium series (2004), 2006Co-Authors: Toyofumi Yamaguchi, Xiaohong Liu, Tsukasa Ogawara, Motoko Inomata, Mineo SaneyoshiAbstract:Telomerase is believed to be a good target for the development of antitumor agents. In this study, 3'-azido-2',3'-dideoxy-2-aminoadenosine (AZddAA), 3'-azido-2',3'-dideoxyadenosine (AZddA), 9-(3-azido-2,3-dideoxy-beta-D-ribofuranosyl)-2-aminopurine (AZddAP), 3'-azido-2-chloro-2',3'-dideoxyadenosine (AZddClA) and their triphosphate derivatives were synthesized. Telomerase assay studies showed that the 2-amino group plays an important role in the inhibitory activity of these compounds. In addition, AZddAA was found to cause Telomere Shortening in of HL60 cells in culture.
Mineo Saneyoshi - One of the best experts on this subject based on the ideXlab platform.
-
3 azido 2 3 dideoxynucleoside 5 triphosphates inhibit telomerase activity in vitro and the corresponding nucleosides cause Telomere Shortening in human hl60 cells
Nucleic Acids Research, 2007Co-Authors: Xiaohong Liu, Tsukasa Ogawara, Mineo Saneyoshi, Hazuki Takahashi, Yoko Harada, Yuta Ogimura, Yoshiyuku Mizushina, Toyofumi YamaguchiAbstract:Telomerase adds telomeric DNA repeats to the ends of linear chromosomal DNA. 3′-Azido-3′-deoxythymidine 5′-triphosphate (AZTTP) is a known telomerase inhibitor. To obtain more selective and potent inhibitors that can be employed as tools for studying telomerase, we investigated the telomerase-inhibitory effects of purine nucleosides bearing a 3′-down azido group: 3′-azido-2′,3′-dideoxyguanosine (AZddG) 5′-triphosphate (AZddGTP), 3′-azido-2′,3′-dideoxy-6-thioguanosine (AZddSG) 5′-triphosphate (AZddSGTP), 3′-azido-2′,3′-dideoxyadenosine (AZddA) 5′-triphosphate (AZddATP) and 3′-azido-2′,3′-dideoxy-2-aminoadenosine (AZddAA) 5′-triphosphate (AZddAATP). Of these, AZddGTP showed the most potent inhibitory activity against HeLa cell telomerase. AZddGTP was significantly incorporated into the 3′-terminus of DNA by partially purified telomerase. However, AZddGTP did not exhibit significant inhibitory activity against DNA polymerases α and δ, suggesting that AZddGTP is a selective inhibitor of telomerase. We also investigated whether long-term treatment with these nucleosides could alter Telomere length and growth rates of human HL60 cells in culture. Southern hybridization analysis of genomic DNA prepared from cells cultured in the presence of AZddG and AZddAA revealed reproducible Telomere Shortening.
-
telomerase inhibition by 3 azido 2 3 dideoxynucleoside 5 triphosphates and Telomere Shortening in human cultured cells by the corresponding nucleosides
Nucleic acids symposium series (2004), 2006Co-Authors: Toyofumi Yamaguchi, Xiaohong Liu, Tsukasa Ogawara, Motoko Inomata, Mineo SaneyoshiAbstract:Telomerase is believed to be a good target for the development of antitumor agents. In this study, 3'-azido-2',3'-dideoxy-2-aminoadenosine (AZddAA), 3'-azido-2',3'-dideoxyadenosine (AZddA), 9-(3-azido-2,3-dideoxy-beta-D-ribofuranosyl)-2-aminopurine (AZddAP), 3'-azido-2-chloro-2',3'-dideoxyadenosine (AZddClA) and their triphosphate derivatives were synthesized. Telomerase assay studies showed that the 2-amino group plays an important role in the inhibitory activity of these compounds. In addition, AZddAA was found to cause Telomere Shortening in of HL60 cells in culture.
Tsukasa Ogawara - One of the best experts on this subject based on the ideXlab platform.
-
3 azido 2 3 dideoxynucleoside 5 triphosphates inhibit telomerase activity in vitro and the corresponding nucleosides cause Telomere Shortening in human hl60 cells
Nucleic Acids Research, 2007Co-Authors: Xiaohong Liu, Tsukasa Ogawara, Mineo Saneyoshi, Hazuki Takahashi, Yoko Harada, Yuta Ogimura, Yoshiyuku Mizushina, Toyofumi YamaguchiAbstract:Telomerase adds telomeric DNA repeats to the ends of linear chromosomal DNA. 3′-Azido-3′-deoxythymidine 5′-triphosphate (AZTTP) is a known telomerase inhibitor. To obtain more selective and potent inhibitors that can be employed as tools for studying telomerase, we investigated the telomerase-inhibitory effects of purine nucleosides bearing a 3′-down azido group: 3′-azido-2′,3′-dideoxyguanosine (AZddG) 5′-triphosphate (AZddGTP), 3′-azido-2′,3′-dideoxy-6-thioguanosine (AZddSG) 5′-triphosphate (AZddSGTP), 3′-azido-2′,3′-dideoxyadenosine (AZddA) 5′-triphosphate (AZddATP) and 3′-azido-2′,3′-dideoxy-2-aminoadenosine (AZddAA) 5′-triphosphate (AZddAATP). Of these, AZddGTP showed the most potent inhibitory activity against HeLa cell telomerase. AZddGTP was significantly incorporated into the 3′-terminus of DNA by partially purified telomerase. However, AZddGTP did not exhibit significant inhibitory activity against DNA polymerases α and δ, suggesting that AZddGTP is a selective inhibitor of telomerase. We also investigated whether long-term treatment with these nucleosides could alter Telomere length and growth rates of human HL60 cells in culture. Southern hybridization analysis of genomic DNA prepared from cells cultured in the presence of AZddG and AZddAA revealed reproducible Telomere Shortening.
-
telomerase inhibition by 3 azido 2 3 dideoxynucleoside 5 triphosphates and Telomere Shortening in human cultured cells by the corresponding nucleosides
Nucleic acids symposium series (2004), 2006Co-Authors: Toyofumi Yamaguchi, Xiaohong Liu, Tsukasa Ogawara, Motoko Inomata, Mineo SaneyoshiAbstract:Telomerase is believed to be a good target for the development of antitumor agents. In this study, 3'-azido-2',3'-dideoxy-2-aminoadenosine (AZddAA), 3'-azido-2',3'-dideoxyadenosine (AZddA), 9-(3-azido-2,3-dideoxy-beta-D-ribofuranosyl)-2-aminopurine (AZddAP), 3'-azido-2-chloro-2',3'-dideoxyadenosine (AZddClA) and their triphosphate derivatives were synthesized. Telomerase assay studies showed that the 2-amino group plays an important role in the inhibitory activity of these compounds. In addition, AZddAA was found to cause Telomere Shortening in of HL60 cells in culture.
