The Experts below are selected from a list of 192234 Experts worldwide ranked by ideXlab platform
Fiona M Frame - One of the best experts on this subject based on the ideXlab platform.
-
low Temperature Plasma treatment induces dna damage leading to necrotic cell death in primary prostate epithelial cells
British Journal of Cancer, 2015Co-Authors: Adam M Hirst, Matthew S Simms, Vincent M Mann, Norman J Maitland, Deborah Oconnell, Fiona M FrameAbstract:Low-Temperature Plasma treatment induces DNA damage leading to necrotic cell death in primary prostate epithelial cells
-
low Temperature Plasma treatment induces dna damage leading to necrotic cell death in primary prostate epithelial cells
British Journal of Cancer, 2015Co-Authors: Adam M Hirst, Matthew S Simms, Vincent M Mann, Norman J Maitland, Deborah Oconnell, Fiona M FrameAbstract:In recent years, the rapidly advancing field of low-Temperature atmospheric pressure Plasmas has shown considerable promise for future translational biomedical applications, including cancer therapy, through the generation of reactive oxygen and nitrogen species. The cytopathic effect of low-Temperature Plasma was first verified in two commonly used prostate cell lines: BPH-1 and PC-3 cells. The study was then extended to analyse the effects in paired normal and tumour (Gleason grade 7) prostate epithelial cells cultured directly from patient tissue. Hydrogen peroxide (H2O2) and staurosporine were used as controls throughout. Low-Temperature Plasma (LTP) exposure resulted in high levels of DNA damage, a reduction in cell viability, and colony-forming ability. H2O2 formed in the culture medium was a likely facilitator of these effects. Necrosis and autophagy were recorded in primary cells, whereas cell lines exhibited apoptosis and necrosis. This study demonstrates that LTP treatment causes cytotoxic insult in primary prostate cells, leading to rapid necrotic cell death. It also highlights the need to study primary cultures in order to gain more realistic insight into patient response.
Adam M Hirst - One of the best experts on this subject based on the ideXlab platform.
-
low Temperature Plasma treatment induces dna damage leading to necrotic cell death in primary prostate epithelial cells
British Journal of Cancer, 2015Co-Authors: Adam M Hirst, Matthew S Simms, Vincent M Mann, Norman J Maitland, Deborah Oconnell, Fiona M FrameAbstract:Low-Temperature Plasma treatment induces DNA damage leading to necrotic cell death in primary prostate epithelial cells
-
low Temperature Plasma treatment induces dna damage leading to necrotic cell death in primary prostate epithelial cells
British Journal of Cancer, 2015Co-Authors: Adam M Hirst, Matthew S Simms, Vincent M Mann, Norman J Maitland, Deborah Oconnell, Fiona M FrameAbstract:In recent years, the rapidly advancing field of low-Temperature atmospheric pressure Plasmas has shown considerable promise for future translational biomedical applications, including cancer therapy, through the generation of reactive oxygen and nitrogen species. The cytopathic effect of low-Temperature Plasma was first verified in two commonly used prostate cell lines: BPH-1 and PC-3 cells. The study was then extended to analyse the effects in paired normal and tumour (Gleason grade 7) prostate epithelial cells cultured directly from patient tissue. Hydrogen peroxide (H2O2) and staurosporine were used as controls throughout. Low-Temperature Plasma (LTP) exposure resulted in high levels of DNA damage, a reduction in cell viability, and colony-forming ability. H2O2 formed in the culture medium was a likely facilitator of these effects. Necrosis and autophagy were recorded in primary cells, whereas cell lines exhibited apoptosis and necrosis. This study demonstrates that LTP treatment causes cytotoxic insult in primary prostate cells, leading to rapid necrotic cell death. It also highlights the need to study primary cultures in order to gain more realistic insight into patient response.
Norman J Maitland - One of the best experts on this subject based on the ideXlab platform.
-
low Temperature Plasma treatment induces dna damage leading to necrotic cell death in primary prostate epithelial cells
British Journal of Cancer, 2015Co-Authors: Adam M Hirst, Matthew S Simms, Vincent M Mann, Norman J Maitland, Deborah Oconnell, Fiona M FrameAbstract:Low-Temperature Plasma treatment induces DNA damage leading to necrotic cell death in primary prostate epithelial cells
-
low Temperature Plasma treatment induces dna damage leading to necrotic cell death in primary prostate epithelial cells
British Journal of Cancer, 2015Co-Authors: Adam M Hirst, Matthew S Simms, Vincent M Mann, Norman J Maitland, Deborah Oconnell, Fiona M FrameAbstract:In recent years, the rapidly advancing field of low-Temperature atmospheric pressure Plasmas has shown considerable promise for future translational biomedical applications, including cancer therapy, through the generation of reactive oxygen and nitrogen species. The cytopathic effect of low-Temperature Plasma was first verified in two commonly used prostate cell lines: BPH-1 and PC-3 cells. The study was then extended to analyse the effects in paired normal and tumour (Gleason grade 7) prostate epithelial cells cultured directly from patient tissue. Hydrogen peroxide (H2O2) and staurosporine were used as controls throughout. Low-Temperature Plasma (LTP) exposure resulted in high levels of DNA damage, a reduction in cell viability, and colony-forming ability. H2O2 formed in the culture medium was a likely facilitator of these effects. Necrosis and autophagy were recorded in primary cells, whereas cell lines exhibited apoptosis and necrosis. This study demonstrates that LTP treatment causes cytotoxic insult in primary prostate cells, leading to rapid necrotic cell death. It also highlights the need to study primary cultures in order to gain more realistic insight into patient response.
Matthew S Simms - One of the best experts on this subject based on the ideXlab platform.
-
low Temperature Plasma treatment induces dna damage leading to necrotic cell death in primary prostate epithelial cells
British Journal of Cancer, 2015Co-Authors: Adam M Hirst, Matthew S Simms, Vincent M Mann, Norman J Maitland, Deborah Oconnell, Fiona M FrameAbstract:Low-Temperature Plasma treatment induces DNA damage leading to necrotic cell death in primary prostate epithelial cells
-
low Temperature Plasma treatment induces dna damage leading to necrotic cell death in primary prostate epithelial cells
British Journal of Cancer, 2015Co-Authors: Adam M Hirst, Matthew S Simms, Vincent M Mann, Norman J Maitland, Deborah Oconnell, Fiona M FrameAbstract:In recent years, the rapidly advancing field of low-Temperature atmospheric pressure Plasmas has shown considerable promise for future translational biomedical applications, including cancer therapy, through the generation of reactive oxygen and nitrogen species. The cytopathic effect of low-Temperature Plasma was first verified in two commonly used prostate cell lines: BPH-1 and PC-3 cells. The study was then extended to analyse the effects in paired normal and tumour (Gleason grade 7) prostate epithelial cells cultured directly from patient tissue. Hydrogen peroxide (H2O2) and staurosporine were used as controls throughout. Low-Temperature Plasma (LTP) exposure resulted in high levels of DNA damage, a reduction in cell viability, and colony-forming ability. H2O2 formed in the culture medium was a likely facilitator of these effects. Necrosis and autophagy were recorded in primary cells, whereas cell lines exhibited apoptosis and necrosis. This study demonstrates that LTP treatment causes cytotoxic insult in primary prostate cells, leading to rapid necrotic cell death. It also highlights the need to study primary cultures in order to gain more realistic insight into patient response.
Vincent M Mann - One of the best experts on this subject based on the ideXlab platform.
-
low Temperature Plasma treatment induces dna damage leading to necrotic cell death in primary prostate epithelial cells
British Journal of Cancer, 2015Co-Authors: Adam M Hirst, Matthew S Simms, Vincent M Mann, Norman J Maitland, Deborah Oconnell, Fiona M FrameAbstract:Low-Temperature Plasma treatment induces DNA damage leading to necrotic cell death in primary prostate epithelial cells
-
low Temperature Plasma treatment induces dna damage leading to necrotic cell death in primary prostate epithelial cells
British Journal of Cancer, 2015Co-Authors: Adam M Hirst, Matthew S Simms, Vincent M Mann, Norman J Maitland, Deborah Oconnell, Fiona M FrameAbstract:In recent years, the rapidly advancing field of low-Temperature atmospheric pressure Plasmas has shown considerable promise for future translational biomedical applications, including cancer therapy, through the generation of reactive oxygen and nitrogen species. The cytopathic effect of low-Temperature Plasma was first verified in two commonly used prostate cell lines: BPH-1 and PC-3 cells. The study was then extended to analyse the effects in paired normal and tumour (Gleason grade 7) prostate epithelial cells cultured directly from patient tissue. Hydrogen peroxide (H2O2) and staurosporine were used as controls throughout. Low-Temperature Plasma (LTP) exposure resulted in high levels of DNA damage, a reduction in cell viability, and colony-forming ability. H2O2 formed in the culture medium was a likely facilitator of these effects. Necrosis and autophagy were recorded in primary cells, whereas cell lines exhibited apoptosis and necrosis. This study demonstrates that LTP treatment causes cytotoxic insult in primary prostate cells, leading to rapid necrotic cell death. It also highlights the need to study primary cultures in order to gain more realistic insight into patient response.
