The Experts below are selected from a list of 171 Experts worldwide ranked by ideXlab platform
Howard C Thomas - One of the best experts on this subject based on the ideXlab platform.
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hypervariable region of hepatitis c virus envelope glycoprotein e2 ns1 in an agammaglobulinemic patient
Gastroenterology, 1994Co-Authors: Umesh Kumar, John Monjardino, Howard C ThomasAbstract:In an agammaglobulinemic patient with chronic hepatitis C, a previously identified hypervariable region of the major envelope glycoprotein remained unchanged for 2.5 years. Serum-derived RNA amplified by reverse transcription-polymerase chain Reaction was cloned in a bacterial vector, and a minimum of three independent clones were sequenced by dideoxy chain Termination Reaction. Comparison of consensus sequences from three different time points during the chronic phase of infection showed absolute homology at both amino acid and nucleotide levels. This finding provides support for the role of antibody selection in generating genetic variation and viral persistence; also, it is consistent with the hypothesis that an epitope within this region is the site of virus neutralization. The observations show that the hepatitis seen in hepatitis C virus infection is not dependent on the humoral immune response.
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Hypervariable Region of Hepatitis C Virus Envelope Glycoprotein (E2/NSl) in an Agammaglobulinemic Patient
1994Co-Authors: Umesh Kumar, John Monjardino, Howard C ThomasAbstract:In an agammaglobulinemic patient with chronic hepatitis C, a previously identified hypervariable region of the major envelope glycoprotein remained unchanged for 2.5 years. Serum-derived RNA amplified by reverse transcription-polymerase chain Reaction was cloned in a bacterial vector, and a minimum of three independent clones were sequenced by dideoxy chain Termination Reaction. Comparison of consensus sequences from three different time points during the chronic phase of infection showed absolute homology at both amino acid and nucleotide levels. This finding provides support for the role of antibody selection in generating genetic variation and viral persistence; also, it is consistent with the hypothesis that an epitope within this region is the site of virus neutralization. The observations show that the hepatitis seen in hepatitis C virus infection is not dependent on the humoral immune response.
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Hypervariable region of hepatitis C virus envelope glycoprotein (E2/NS1) in an agammaglobulinemic patient.
Gastroenterology, 1994Co-Authors: Umesh Kumar, John Monjardino, Howard C ThomasAbstract:In an agammaglobulinemic patient with chronic hepatitis C, a previously identified hypervariable region of the major envelope glycoprotein remained unchanged for 2.5 years. Serum-derived RNA amplified by reverse transcription-polymerase chain Reaction was cloned in a bacterial vector, and a minimum of three independent clones were sequenced by dideoxy chain Termination Reaction. Comparison of consensus sequences from three different time points during the chronic phase of infection showed absolute homology at both amino acid and nucleotide levels. This finding provides support for the role of antibody selection in generating genetic variation and viral persistence; also, it is consistent with the hypothesis that an epitope within this region is the site of virus neutralization. The observations show that the hepatitis seen in hepatitis C virus infection is not dependent on the humoral immune response.
Shigeru Yamago - One of the best experts on this subject based on the ideXlab platform.
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The Effect of Viscosity on the Coupling and Hydrogen-Abstraction Reaction between Transient and Persistent Radicals
Bulletin of the Chemical Society of Japan, 2021Co-Authors: Tatsuhisa Kato, Yasuyuki Nakamura, Shigeru YamagoAbstract:The effect of viscosity on the radical Termination Reaction between a transient radical and a persistent radical undergoing a coupling Reaction (Coup) or hydrogen abstraction (Abst) was examined. I...
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Evidence for Polarity- and Viscosity-Controlled Domains in the Termination Reaction in the Radical Polymerization of Acrylonitrile
2021Co-Authors: Xiaopei Lin, Tasuku Ogihara, Tatsuhisa Kato, Yasuyuki Nakamura, Shigeru YamagoAbstract:The Termination mechanism in the radical polymerization of acrylonitrile (AN) was determined by the Reaction of structurally well-defined polyacryronitrile (PAN) chain-end radical <b>1a</b> and PAN-end mimetic small model radical <b>1b</b>. The contributions of three mechanisms, i.e., the disproportionation (<i>Disp</i>), the combination by carbon-carbon formation (<i>CC-Comb</i>), and the combination by carbon-nitrogen bond formation (<i>CN-Comb</i>), were unambiguously determined. The <i>CN-Comb</i> pathway was experimentally proved for the first time. The selectivity between <i>Disp </i>and<i> CC-Comb</i> showed a good correlation with the viscosity and temperature, and the <i>Disp</i> selectivity increased as the viscosity increased, as previously reported for the Termination of other monomers. In contrast, <i>CN-Comb</i> is insensitive to viscosity but sensitive to polarity; the selectivity decreases as the polarity of the media increases. The results strongly suggest the presence of two domains in the Termination Reaction, namely, the polarity- and viscosity-controlled domains. <i>CN-Comb</i> product<b> 5</b> was stable under the polymerization conditions but decomposed to <i>Disp</i> and <i>CC-Comb</i> products at high temperatures. Therefore, care must be taken in the processing step, such as the molding process, because the physical properties could be altered due to changes in the <i>Disp</i> and <i>CC-Comb</i> compositions.
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evidence for polarity and viscosity controlled pathways in the Termination Reaction in the radical polymerization of acrylonitrile
Macromolecules, 2021Co-Authors: Xiaopei Lin, Tasuku Ogihara, Tatsuhisa Kato, Yasuyuki Nakamura, Shigeru YamagoAbstract:The Termination mechanism in the radical polymerization of acrylonitrile (AN) was determined through the product analysis for the Reaction of structurally well-defined polyacrylonitrile (PAN) chain...
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The Effect of Viscosity on the Diffusion and Termination Reaction of Organic Radical Pairs.
Chemistry (Weinheim an der Bergstrasse Germany), 2019Co-Authors: Tasuku Ogihara, Yasuyuki Nakamura, Manabu Abe, Shigeru YamagoAbstract:The effect of viscosity on the diffusion efficiency (Fdif ) of an organic radical pair in a solvent cage and the Termination mechanism, that is, the selectivity of disproportionation (Disp) and combination (Comb) of the geminated caged radical pair and the diffused radicals encountered, were investigated quantitatively by following the photolysis of dimethyl 2,2'-azobis(2-methylpropionate) (V-601) in the absence and presence of PhSD. Fdif and Disp/Comb selectivity outside the cage [Disp(dif) /Comb(dif) ] are highly sensitive to the viscosity. In contrast, the Disp/Comb selectivity inside the cage [Disp(cage) /Comb(cage) ] is rather insensitive. The difference in viscosity dependence between Disp(cage) /Comb(cage) and Disp(dif) /Comb(dif) is explained by the spin state of the radical pair inside and outside the cage and the spin state dependent configurational changes of the radical pair upon their collision. Given that the configurational change of the radicals associates the displacement and reorganization of solvents around the radicals, the Termination outside the cage, which requires larger change than that inside the cage, is highly viscosity dependent. Furthermore, while the bulk viscosity of each solvent shows good correlation with Fdif and Disp/Comb selectivity, microviscosity is the better parameter predicting Fdif and Disp(dif) /Comb(dif) selectivity regardless of the solvents.
Umesh Kumar - One of the best experts on this subject based on the ideXlab platform.
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hypervariable region of hepatitis c virus envelope glycoprotein e2 ns1 in an agammaglobulinemic patient
Gastroenterology, 1994Co-Authors: Umesh Kumar, John Monjardino, Howard C ThomasAbstract:In an agammaglobulinemic patient with chronic hepatitis C, a previously identified hypervariable region of the major envelope glycoprotein remained unchanged for 2.5 years. Serum-derived RNA amplified by reverse transcription-polymerase chain Reaction was cloned in a bacterial vector, and a minimum of three independent clones were sequenced by dideoxy chain Termination Reaction. Comparison of consensus sequences from three different time points during the chronic phase of infection showed absolute homology at both amino acid and nucleotide levels. This finding provides support for the role of antibody selection in generating genetic variation and viral persistence; also, it is consistent with the hypothesis that an epitope within this region is the site of virus neutralization. The observations show that the hepatitis seen in hepatitis C virus infection is not dependent on the humoral immune response.
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Hypervariable Region of Hepatitis C Virus Envelope Glycoprotein (E2/NSl) in an Agammaglobulinemic Patient
1994Co-Authors: Umesh Kumar, John Monjardino, Howard C ThomasAbstract:In an agammaglobulinemic patient with chronic hepatitis C, a previously identified hypervariable region of the major envelope glycoprotein remained unchanged for 2.5 years. Serum-derived RNA amplified by reverse transcription-polymerase chain Reaction was cloned in a bacterial vector, and a minimum of three independent clones were sequenced by dideoxy chain Termination Reaction. Comparison of consensus sequences from three different time points during the chronic phase of infection showed absolute homology at both amino acid and nucleotide levels. This finding provides support for the role of antibody selection in generating genetic variation and viral persistence; also, it is consistent with the hypothesis that an epitope within this region is the site of virus neutralization. The observations show that the hepatitis seen in hepatitis C virus infection is not dependent on the humoral immune response.
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Hypervariable region of hepatitis C virus envelope glycoprotein (E2/NS1) in an agammaglobulinemic patient.
Gastroenterology, 1994Co-Authors: Umesh Kumar, John Monjardino, Howard C ThomasAbstract:In an agammaglobulinemic patient with chronic hepatitis C, a previously identified hypervariable region of the major envelope glycoprotein remained unchanged for 2.5 years. Serum-derived RNA amplified by reverse transcription-polymerase chain Reaction was cloned in a bacterial vector, and a minimum of three independent clones were sequenced by dideoxy chain Termination Reaction. Comparison of consensus sequences from three different time points during the chronic phase of infection showed absolute homology at both amino acid and nucleotide levels. This finding provides support for the role of antibody selection in generating genetic variation and viral persistence; also, it is consistent with the hypothesis that an epitope within this region is the site of virus neutralization. The observations show that the hepatitis seen in hepatitis C virus infection is not dependent on the humoral immune response.
Johan Åqvist - One of the best experts on this subject based on the ideXlab platform.
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Mechanism of the translation Termination Reaction on the ribosome
Biochemistry, 2009Co-Authors: Stefan Trobro, Johan ÅqvistAbstract:Ribosomal release factors (RFs) catalyze the Termination of protein synthesis by triggering hydrolysis of the peptidyl−tRNA ester bond in the peptidyl transferase center of the ribosome. With new medium-resolution crystallographic structures of RF−ribosome complexes available, it has become possible to examine the detailed mechanism of this process to resolve the key factors responsible for catalysis of the Termination Reaction. Here, we report computer simulations of the Termination Reaction that utilize both the new RF complex structures and information from a high-resolution complex with a P-site substrate analogue. The calculations yield a consistent Reaction mechanism that reproduces experimental rates and allows us to identify key interactions responsible for the catalytic efficiency. The results are also in general agreement with an earlier model based on molecular docking. The methylated glutamine residue of the universally conserved GGQ motif plays a key role in the hydrolysis Reaction by orienti...
John Monjardino - One of the best experts on this subject based on the ideXlab platform.
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hypervariable region of hepatitis c virus envelope glycoprotein e2 ns1 in an agammaglobulinemic patient
Gastroenterology, 1994Co-Authors: Umesh Kumar, John Monjardino, Howard C ThomasAbstract:In an agammaglobulinemic patient with chronic hepatitis C, a previously identified hypervariable region of the major envelope glycoprotein remained unchanged for 2.5 years. Serum-derived RNA amplified by reverse transcription-polymerase chain Reaction was cloned in a bacterial vector, and a minimum of three independent clones were sequenced by dideoxy chain Termination Reaction. Comparison of consensus sequences from three different time points during the chronic phase of infection showed absolute homology at both amino acid and nucleotide levels. This finding provides support for the role of antibody selection in generating genetic variation and viral persistence; also, it is consistent with the hypothesis that an epitope within this region is the site of virus neutralization. The observations show that the hepatitis seen in hepatitis C virus infection is not dependent on the humoral immune response.
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Hypervariable Region of Hepatitis C Virus Envelope Glycoprotein (E2/NSl) in an Agammaglobulinemic Patient
1994Co-Authors: Umesh Kumar, John Monjardino, Howard C ThomasAbstract:In an agammaglobulinemic patient with chronic hepatitis C, a previously identified hypervariable region of the major envelope glycoprotein remained unchanged for 2.5 years. Serum-derived RNA amplified by reverse transcription-polymerase chain Reaction was cloned in a bacterial vector, and a minimum of three independent clones were sequenced by dideoxy chain Termination Reaction. Comparison of consensus sequences from three different time points during the chronic phase of infection showed absolute homology at both amino acid and nucleotide levels. This finding provides support for the role of antibody selection in generating genetic variation and viral persistence; also, it is consistent with the hypothesis that an epitope within this region is the site of virus neutralization. The observations show that the hepatitis seen in hepatitis C virus infection is not dependent on the humoral immune response.
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Hypervariable region of hepatitis C virus envelope glycoprotein (E2/NS1) in an agammaglobulinemic patient.
Gastroenterology, 1994Co-Authors: Umesh Kumar, John Monjardino, Howard C ThomasAbstract:In an agammaglobulinemic patient with chronic hepatitis C, a previously identified hypervariable region of the major envelope glycoprotein remained unchanged for 2.5 years. Serum-derived RNA amplified by reverse transcription-polymerase chain Reaction was cloned in a bacterial vector, and a minimum of three independent clones were sequenced by dideoxy chain Termination Reaction. Comparison of consensus sequences from three different time points during the chronic phase of infection showed absolute homology at both amino acid and nucleotide levels. This finding provides support for the role of antibody selection in generating genetic variation and viral persistence; also, it is consistent with the hypothesis that an epitope within this region is the site of virus neutralization. The observations show that the hepatitis seen in hepatitis C virus infection is not dependent on the humoral immune response.
