The Experts below are selected from a list of 261996 Experts worldwide ranked by ideXlab platform
Ronald N. Jones - One of the best experts on this subject based on the ideXlab platform.
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haemophilus Test Medium interpretive criteria for disk diffusion susceptibility Tests with cefdinir cefetamet cefmetazole cefpodoxime cefdaloxime ru29246 hr 916 metabolite and trospectomycin
Diagnostic Microbiology and Infectious Disease, 1992Co-Authors: Ronald N. Jones, Meridith E ErwinAbstract:Abstract The disk diffusion zones and the MICs of six newer antimicrobials with significant activity against Haemophilus influenzae were compared using the Haemophilus Test Medium (HTM) and National Committee for Clinical Laboratory Standards methods. The rank order of potency was cefpodoxime ( MIC 50 , ≤0.03 μ g/ml) > cefetamet > cefdinir > cefdaloxime=trospectomycin > cefmetazole ( MIC 50 , 2 μ g/ml). Susceptible breakpoint interpretive criteria for HTM Tests were established that conformed to prior recommendations for each drug when Tested against other species. Absolute agreement between methods ranged from 89% to 100% with ≤1% false-susceptible interpretive errors. The recommended, tentative disk diffusion susceptible interpretive criteria were for 5-μg cefdinir disks ≥20 mm (MIC correlate, ≤1 μ g/ml); for 10-μg cefetamet disks ≥18 mm (MIC correlate, ≤4 μ g/ml); for 30-μg cefetamet disks ≥21 mm (MIC correlate, ≤4 μ g/ml); for 30-μg cefmetazole disks ≥16 mm (MIC correlate, ≤16 μ g/ml); for 10-μg cefpodoxime disks ≥21 mm (MIC correlate, ≤2 μ g/ml); for 30-μg cefdaloxime disks ≥23 mm (MIC correlate, ≤2 μ g/ml) and for 30-μg trospectomycin disks ≥17 mm (MIC correlate, ≤16 μ g/ml). β-Lactamase-negative, ampicillin-resistant (BLNAR) H. influenzae isolates consistently had the highest MICs for each cephalosporin Tested. These BLNAR strains most influenced the perceived in vitro spectrum of each β-lactam Tested and required a recommendation of intermediate and resistant categories for cefdinir, cefetamet, and cefdaloxime.
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interpretive criteria for ci 960 fleroxacin and temafloxacin susceptibility Tests withhaemophilus influenzae
European Journal of Clinical Microbiology & Infectious Diseases, 1992Co-Authors: Mary S Barrett, Ronald N. JonesAbstract:Haemophilus influenzae strains with varied ampicillin resistance and beta-lactamase production patterns were Tested against three investigational fluorinated quinolones (CI-960, fleroxacin, temafloxacin) using Haemophilus Test Medium (HTM) and National Committee for Clinical Laboratory Standards (NCCLS) methods. The disk diffusion zones and MICs were compared and regression statistics and scattergrams generated. The rank order of the agents according to activity againstHaemophilus influenzae was CI-960 (MIC50 0.002 µg/ml) > temafloxacin (MIC50 0.015 µg/ml) > fleroxacin (MIC50 0.03 µg/ml). The recommended susceptibility interpretive criteria for the 5-µg disks of each drug were: for CI-960 ≥ 23 mm (MIC correlate ≤ 1 µg/ml); for fleroxacin ≥ 19 mm (MIC correlate ≤ 2 µg/ml); and for temafloxacin ≥ 16 mm (MIC correlate ≤ 2 µg/ml). All recentHaemophilus influenzae isolates Tested were susceptible to these potent fluoroquinolones and no interpretive errors were observed.
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disk diffusion quality control guidelines for haemophilus susceptibility Tests using cefdinir ci 960 fleroxacin temafloxacin and trospectomycin
Journal of Clinical Microbiology, 1992Co-Authors: M J Bale, Ronald N. Jones, M E Erwin, F P Koontz, E H Gerlach, P. R. Murray, J A WashingtonAbstract:A multilaboratory study to determine disk diffusion quality control ranges for Haemophilus influenzae ATCC 49247 and five investigational drugs was performed. Multiple lots of Haemophilus Test Medium and antibiotic disks were used for replicate Testing in conformance with the recommendations of the National Committee for Clinical Laboratory Standards. Quality control disk zone diameter ranges were proposed for cefdinir, CI-960, fleroxacin, temafloxacin, and trospectomycin.
Thomas V Riley - One of the best experts on this subject based on the ideXlab platform.
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susceptibility of transient and commensal skin flora to the essential oil of melaleuca alternifolia tea tree oil
American Journal of Infection Control, 1996Co-Authors: Katherine A Hammer, Christine F Carson, Thomas V RileyAbstract:Abstract Objectives: The purpose of this study was to determine the susceptibility of a range of transient and commensal skin flora to the essential oil of Melaleuca alternifolia , or tea tree. Methods: A modified broth microdilution method was used. Polyoxyethylene sorbitan mono-oleate detergent was added to the Test Medium to enhance solubility of the tea tree oil. Results: Serratia marcescens had the lowest minimum inhibitory concentration (MIC 90 ) of 0.25%. The highest MIC 90 was 3% for Pseudomonas aeruginosa . The lowest minimum bactericidal concentration (MBC 90 ) was 0.25% for S. marcescens and Klebsiella pneumoniae , whereas the highest was 8% for Staphylococcus capitis . Conclusions: S. aureus and most of the gram-negative bacteria Tested were more susceptible to tea tree oil than the coagulase-negative staphylocci and micrococci. These results suggest that tea tree oil may be useful in removing transient skin flora while suppressing but maintaining resident flora.
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broth micro dilution method for determining the susceptibility of escherichia coli and staphylococcus aureus to the essential oil of melaleuca alternifolia tea tree oil
Microbios, 1995Co-Authors: Christine F Carson, Katherine A Hammer, Thomas V RileyAbstract:A broth micro-dilution method was used to examine the susceptibility of Escherichia coli (n = 110) and Staphylococcus aureus (n = 105) to the essential oil of Melaleuca alternifolia (tea tree oil). The detergent Tween 80 was used successfully to enhance the solubility of tea tree oil in the Test Medium. The MIC90 of tea tree oil for E. coli was 0.25% while for S. aureus it was 0.50%.
J A Washington - One of the best experts on this subject based on the ideXlab platform.
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disk diffusion quality control guidelines for haemophilus susceptibility Tests using cefdinir ci 960 fleroxacin temafloxacin and trospectomycin
Journal of Clinical Microbiology, 1992Co-Authors: M J Bale, Ronald N. Jones, M E Erwin, F P Koontz, E H Gerlach, P. R. Murray, J A WashingtonAbstract:A multilaboratory study to determine disk diffusion quality control ranges for Haemophilus influenzae ATCC 49247 and five investigational drugs was performed. Multiple lots of Haemophilus Test Medium and antibiotic disks were used for replicate Testing in conformance with the recommendations of the National Committee for Clinical Laboratory Standards. Quality control disk zone diameter ranges were proposed for cefdinir, CI-960, fleroxacin, temafloxacin, and trospectomycin.
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mic quality control guidelines for haemophilus susceptibility Tests using cefdinir fk482 cefepime cefetamet cefpirome ceftibuten fleroxacin temafloxacin clarithromycin rp59500 and trospectomycin
Journal of Clinical Microbiology, 1992Co-Authors: M J Bale, M E Erwin, F P Koontz, E H Gerlach, P. R. Murray, R N Jones, J A WashingtonAbstract:A multilaboratory study was performed to establish broth microdilution MIC quality control (QC) guidelines for 10 investigational drugs which previously demonstrated significant activity against Haemophilus influenzae. MIC QC ranges for H. influenzae ATCC 49247 with Haemophilus Test Medium were determined by using multiple contemporary lots of Haemophilus Test Medium and the National Committee for Clinical Laboratory Standards' recommended numbers of replicate Tests. On the basis of these results, QC ranges (generally modal MIC +/- one log2 dilution) are proposed for cefdinir, cefepime, cefetamet, cefpirome, ceftibuten, fleroxacin, temafloxacin, clarithromycin, RP59500, and trospectomycin. The proposed QC guidelines for clarithromycin and temafloxacin were recently accepted by the National Committee for Clinical Laboratory Standards.
E H Gerlach - One of the best experts on this subject based on the ideXlab platform.
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revised disk diffusion interpretive criteria for cefaclor loracarbef cefprozil and cefixime when Testinghaemophilus influenzae on haemophilus Test Medium
European Journal of Clinical Microbiology & Infectious Diseases, 1994Co-Authors: Gary V Doern, E H Gerlach, R N Jones, Janet Hindler, R St AmandAbstract:The aim of the current five-center collaborative study was to reassess the interpretive criteria for cefaclor, loracarbef, cefprozil and cefixime previously adopted or proposed by the National Committee for Clinical Laboratory Standards (NCCLS) for disk diffusion susceptibility Tests withHaemophilus influenzae on Haemophilus Test Medium (HTM) agar. MICs and zones of inhibition were determined using NCCLS methods, HTM and two collections of strains ofHaemophilus influenzae. One group of strains consisted of 118 stock organisms taken largely from various recent U.S. antibiotic resistance surveillance studies. The emphasis in this selected group of organisms was on strains that were beta-lactamase negative but ampicillin resistant (BLNAR) by some other mechanism. The second collection of Test organisms consisted of 50 recent clinical isolates ofHaemophilus influenzae obtained from each of the five participating study centers. This group was considered representative of the type ofHaemophilus influenzae currently recovered from clinical sources in the USA. Frequency distribution assessment and error-rate bounded analysis of scattergram comparisons of MICs and zone sizes were used to develop the following zone diameters interpretive for disk diffusion susceptibility Tests withHaemophilus influenzae on HTM agar: cefaclor, ≥20 mm (susceptible, S) and ≤16 mm (resistant, R); loracarbef, ≥19 mm (S) and ≤15 mm (R); and cefprozil, >18 mm (S) and <14 mm (R). The respective MIC correlates for all three antimicrobial agents were ≤8 µg/ml (S) and 32 µg/ml (R). For cefixime, a zone diameter size of ≥21 mm was recommended to define the susceptible category, with an MIC correlate of ≤1 µg/ml. Only a susceptible category was defined for cefixime Testing. These new interpretive criteria have recently been adopted by the NCCLS.
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disk diffusion quality control guidelines for haemophilus susceptibility Tests using cefdinir ci 960 fleroxacin temafloxacin and trospectomycin
Journal of Clinical Microbiology, 1992Co-Authors: M J Bale, Ronald N. Jones, M E Erwin, F P Koontz, E H Gerlach, P. R. Murray, J A WashingtonAbstract:A multilaboratory study to determine disk diffusion quality control ranges for Haemophilus influenzae ATCC 49247 and five investigational drugs was performed. Multiple lots of Haemophilus Test Medium and antibiotic disks were used for replicate Testing in conformance with the recommendations of the National Committee for Clinical Laboratory Standards. Quality control disk zone diameter ranges were proposed for cefdinir, CI-960, fleroxacin, temafloxacin, and trospectomycin.
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mic quality control guidelines for haemophilus susceptibility Tests using cefdinir fk482 cefepime cefetamet cefpirome ceftibuten fleroxacin temafloxacin clarithromycin rp59500 and trospectomycin
Journal of Clinical Microbiology, 1992Co-Authors: M J Bale, M E Erwin, F P Koontz, E H Gerlach, P. R. Murray, R N Jones, J A WashingtonAbstract:A multilaboratory study was performed to establish broth microdilution MIC quality control (QC) guidelines for 10 investigational drugs which previously demonstrated significant activity against Haemophilus influenzae. MIC QC ranges for H. influenzae ATCC 49247 with Haemophilus Test Medium were determined by using multiple contemporary lots of Haemophilus Test Medium and the National Committee for Clinical Laboratory Standards' recommended numbers of replicate Tests. On the basis of these results, QC ranges (generally modal MIC +/- one log2 dilution) are proposed for cefdinir, cefepime, cefetamet, cefpirome, ceftibuten, fleroxacin, temafloxacin, clarithromycin, RP59500, and trospectomycin. The proposed QC guidelines for clarithromycin and temafloxacin were recently accepted by the National Committee for Clinical Laboratory Standards.
Katherine A Hammer - One of the best experts on this subject based on the ideXlab platform.
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susceptibility of transient and commensal skin flora to the essential oil of melaleuca alternifolia tea tree oil
American Journal of Infection Control, 1996Co-Authors: Katherine A Hammer, Christine F Carson, Thomas V RileyAbstract:Abstract Objectives: The purpose of this study was to determine the susceptibility of a range of transient and commensal skin flora to the essential oil of Melaleuca alternifolia , or tea tree. Methods: A modified broth microdilution method was used. Polyoxyethylene sorbitan mono-oleate detergent was added to the Test Medium to enhance solubility of the tea tree oil. Results: Serratia marcescens had the lowest minimum inhibitory concentration (MIC 90 ) of 0.25%. The highest MIC 90 was 3% for Pseudomonas aeruginosa . The lowest minimum bactericidal concentration (MBC 90 ) was 0.25% for S. marcescens and Klebsiella pneumoniae , whereas the highest was 8% for Staphylococcus capitis . Conclusions: S. aureus and most of the gram-negative bacteria Tested were more susceptible to tea tree oil than the coagulase-negative staphylocci and micrococci. These results suggest that tea tree oil may be useful in removing transient skin flora while suppressing but maintaining resident flora.
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broth micro dilution method for determining the susceptibility of escherichia coli and staphylococcus aureus to the essential oil of melaleuca alternifolia tea tree oil
Microbios, 1995Co-Authors: Christine F Carson, Katherine A Hammer, Thomas V RileyAbstract:A broth micro-dilution method was used to examine the susceptibility of Escherichia coli (n = 110) and Staphylococcus aureus (n = 105) to the essential oil of Melaleuca alternifolia (tea tree oil). The detergent Tween 80 was used successfully to enhance the solubility of tea tree oil in the Test Medium. The MIC90 of tea tree oil for E. coli was 0.25% while for S. aureus it was 0.50%.
