The Experts below are selected from a list of 78 Experts worldwide ranked by ideXlab platform
M C Hardegree - One of the best experts on this subject based on the ideXlab platform.
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Transmembrane Diffusion Channels inMycoplasma gallisepticum Induced byTetanolysin
1990Co-Authors: Shlomo Rottem, K Groover, W H Habig, Maria Barile, M C HardegreeAbstract:Thepermeability properties ofMycoplasma gallisepticum cells treated witha purified preparation of Tetanolysin were investigated bydetermining theinitial swelling ratesofcells suspended inan isoosmotic solution ofelectrolytes or nonelectrolytes. Theswelling, initiated bytheTetanolysin, depended on the Tetanolysin concentration andwas markedly affected bythemolecular size ofthevarious osmotic stabilizers utilized. Thus,theinitial swelling rates inan isoosmotic solution ofmonosaccharides were muchhigher than those inisoosmotic solutions ofdi-, tri-, ortetrasaccharides. Cellswelling induced byTetanolysin was much lower withenergy-depleted M.gallisepticum cells, witharsenate-treated cells, orwhenthemembrane potential (A+)was collapsed byvalinomycin (10,uM)plusKCI(100mM).Swelling was notaffected bythe
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transmembrane diffusion channels in mycoplasma gallisepticum induced by Tetanolysin
Infection and Immunity, 1990Co-Authors: Shlomo Rottem, K Groover, W H Habig, Maria Barile, M C HardegreeAbstract:The permeability properties of Mycoplasma gallisepticum cells treated with a purified preparation of Tetanolysin were investigated by determining the initial swelling rates of cells suspended in an isoosmotic solution of electrolytes or nonelectrolytes. The swelling, initiated by the Tetanolysin, depended on the Tetanolysin concentration and was markedly affected by the molecular size of the various osmotic stabilizers utilized. Thus, the initial swelling rates in an isoosmotic solution of monosaccharides were much higher than those in isoosmotic solutions of di-, tri-, or tetrasaccharides. Cell swelling induced by Tetanolysin was much lower with energy-depleted M. gallisepticum cells, with arsenate-treated cells, or when the membrane potential (delta psi) was collapsed by valinomycin (10 microM) plus KCl (100 mM). Swelling was not affected by the proton-conducting ionophore carbonyl cyanide-m-chlorophenylhydrazone (1 to 10 microM) or by nigericin (5 microM). These results support the concept that the damage induced by Tetanolysin is due to the formation of water-filled pores within the membranes of energized M. gallisepticum cells. Such pores allow the diffusion of hydrophilic molecules into the cells and may vary in size, depending on the Tetanolysin concentration utilized.
Shlomo Rottem - One of the best experts on this subject based on the ideXlab platform.
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Transmembrane Diffusion Channels inMycoplasma gallisepticum Induced byTetanolysin
1990Co-Authors: Shlomo Rottem, K Groover, W H Habig, Maria Barile, M C HardegreeAbstract:Thepermeability properties ofMycoplasma gallisepticum cells treated witha purified preparation of Tetanolysin were investigated bydetermining theinitial swelling ratesofcells suspended inan isoosmotic solution ofelectrolytes or nonelectrolytes. Theswelling, initiated bytheTetanolysin, depended on the Tetanolysin concentration andwas markedly affected bythemolecular size ofthevarious osmotic stabilizers utilized. Thus,theinitial swelling rates inan isoosmotic solution ofmonosaccharides were muchhigher than those inisoosmotic solutions ofdi-, tri-, ortetrasaccharides. Cellswelling induced byTetanolysin was much lower withenergy-depleted M.gallisepticum cells, witharsenate-treated cells, orwhenthemembrane potential (A+)was collapsed byvalinomycin (10,uM)plusKCI(100mM).Swelling was notaffected bythe
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transmembrane diffusion channels in mycoplasma gallisepticum induced by Tetanolysin
Infection and Immunity, 1990Co-Authors: Shlomo Rottem, K Groover, W H Habig, Maria Barile, M C HardegreeAbstract:The permeability properties of Mycoplasma gallisepticum cells treated with a purified preparation of Tetanolysin were investigated by determining the initial swelling rates of cells suspended in an isoosmotic solution of electrolytes or nonelectrolytes. The swelling, initiated by the Tetanolysin, depended on the Tetanolysin concentration and was markedly affected by the molecular size of the various osmotic stabilizers utilized. Thus, the initial swelling rates in an isoosmotic solution of monosaccharides were much higher than those in isoosmotic solutions of di-, tri-, or tetrasaccharides. Cell swelling induced by Tetanolysin was much lower with energy-depleted M. gallisepticum cells, with arsenate-treated cells, or when the membrane potential (delta psi) was collapsed by valinomycin (10 microM) plus KCl (100 mM). Swelling was not affected by the proton-conducting ionophore carbonyl cyanide-m-chlorophenylhydrazone (1 to 10 microM) or by nigericin (5 microM). These results support the concept that the damage induced by Tetanolysin is due to the formation of water-filled pores within the membranes of energized M. gallisepticum cells. Such pores allow the diffusion of hydrophilic molecules into the cells and may vary in size, depending on the Tetanolysin concentration utilized.
K Groover - One of the best experts on this subject based on the ideXlab platform.
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Transmembrane Diffusion Channels inMycoplasma gallisepticum Induced byTetanolysin
1990Co-Authors: Shlomo Rottem, K Groover, W H Habig, Maria Barile, M C HardegreeAbstract:Thepermeability properties ofMycoplasma gallisepticum cells treated witha purified preparation of Tetanolysin were investigated bydetermining theinitial swelling ratesofcells suspended inan isoosmotic solution ofelectrolytes or nonelectrolytes. Theswelling, initiated bytheTetanolysin, depended on the Tetanolysin concentration andwas markedly affected bythemolecular size ofthevarious osmotic stabilizers utilized. Thus,theinitial swelling rates inan isoosmotic solution ofmonosaccharides were muchhigher than those inisoosmotic solutions ofdi-, tri-, ortetrasaccharides. Cellswelling induced byTetanolysin was much lower withenergy-depleted M.gallisepticum cells, witharsenate-treated cells, orwhenthemembrane potential (A+)was collapsed byvalinomycin (10,uM)plusKCI(100mM).Swelling was notaffected bythe
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transmembrane diffusion channels in mycoplasma gallisepticum induced by Tetanolysin
Infection and Immunity, 1990Co-Authors: Shlomo Rottem, K Groover, W H Habig, Maria Barile, M C HardegreeAbstract:The permeability properties of Mycoplasma gallisepticum cells treated with a purified preparation of Tetanolysin were investigated by determining the initial swelling rates of cells suspended in an isoosmotic solution of electrolytes or nonelectrolytes. The swelling, initiated by the Tetanolysin, depended on the Tetanolysin concentration and was markedly affected by the molecular size of the various osmotic stabilizers utilized. Thus, the initial swelling rates in an isoosmotic solution of monosaccharides were much higher than those in isoosmotic solutions of di-, tri-, or tetrasaccharides. Cell swelling induced by Tetanolysin was much lower with energy-depleted M. gallisepticum cells, with arsenate-treated cells, or when the membrane potential (delta psi) was collapsed by valinomycin (10 microM) plus KCl (100 mM). Swelling was not affected by the proton-conducting ionophore carbonyl cyanide-m-chlorophenylhydrazone (1 to 10 microM) or by nigericin (5 microM). These results support the concept that the damage induced by Tetanolysin is due to the formation of water-filled pores within the membranes of energized M. gallisepticum cells. Such pores allow the diffusion of hydrophilic molecules into the cells and may vary in size, depending on the Tetanolysin concentration utilized.
W H Habig - One of the best experts on this subject based on the ideXlab platform.
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Transmembrane Diffusion Channels inMycoplasma gallisepticum Induced byTetanolysin
1990Co-Authors: Shlomo Rottem, K Groover, W H Habig, Maria Barile, M C HardegreeAbstract:Thepermeability properties ofMycoplasma gallisepticum cells treated witha purified preparation of Tetanolysin were investigated bydetermining theinitial swelling ratesofcells suspended inan isoosmotic solution ofelectrolytes or nonelectrolytes. Theswelling, initiated bytheTetanolysin, depended on the Tetanolysin concentration andwas markedly affected bythemolecular size ofthevarious osmotic stabilizers utilized. Thus,theinitial swelling rates inan isoosmotic solution ofmonosaccharides were muchhigher than those inisoosmotic solutions ofdi-, tri-, ortetrasaccharides. Cellswelling induced byTetanolysin was much lower withenergy-depleted M.gallisepticum cells, witharsenate-treated cells, orwhenthemembrane potential (A+)was collapsed byvalinomycin (10,uM)plusKCI(100mM).Swelling was notaffected bythe
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transmembrane diffusion channels in mycoplasma gallisepticum induced by Tetanolysin
Infection and Immunity, 1990Co-Authors: Shlomo Rottem, K Groover, W H Habig, Maria Barile, M C HardegreeAbstract:The permeability properties of Mycoplasma gallisepticum cells treated with a purified preparation of Tetanolysin were investigated by determining the initial swelling rates of cells suspended in an isoosmotic solution of electrolytes or nonelectrolytes. The swelling, initiated by the Tetanolysin, depended on the Tetanolysin concentration and was markedly affected by the molecular size of the various osmotic stabilizers utilized. Thus, the initial swelling rates in an isoosmotic solution of monosaccharides were much higher than those in isoosmotic solutions of di-, tri-, or tetrasaccharides. Cell swelling induced by Tetanolysin was much lower with energy-depleted M. gallisepticum cells, with arsenate-treated cells, or when the membrane potential (delta psi) was collapsed by valinomycin (10 microM) plus KCl (100 mM). Swelling was not affected by the proton-conducting ionophore carbonyl cyanide-m-chlorophenylhydrazone (1 to 10 microM) or by nigericin (5 microM). These results support the concept that the damage induced by Tetanolysin is due to the formation of water-filled pores within the membranes of energized M. gallisepticum cells. Such pores allow the diffusion of hydrophilic molecules into the cells and may vary in size, depending on the Tetanolysin concentration utilized.
Maria Barile - One of the best experts on this subject based on the ideXlab platform.
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Transmembrane Diffusion Channels inMycoplasma gallisepticum Induced byTetanolysin
1990Co-Authors: Shlomo Rottem, K Groover, W H Habig, Maria Barile, M C HardegreeAbstract:Thepermeability properties ofMycoplasma gallisepticum cells treated witha purified preparation of Tetanolysin were investigated bydetermining theinitial swelling ratesofcells suspended inan isoosmotic solution ofelectrolytes or nonelectrolytes. Theswelling, initiated bytheTetanolysin, depended on the Tetanolysin concentration andwas markedly affected bythemolecular size ofthevarious osmotic stabilizers utilized. Thus,theinitial swelling rates inan isoosmotic solution ofmonosaccharides were muchhigher than those inisoosmotic solutions ofdi-, tri-, ortetrasaccharides. Cellswelling induced byTetanolysin was much lower withenergy-depleted M.gallisepticum cells, witharsenate-treated cells, orwhenthemembrane potential (A+)was collapsed byvalinomycin (10,uM)plusKCI(100mM).Swelling was notaffected bythe
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transmembrane diffusion channels in mycoplasma gallisepticum induced by Tetanolysin
Infection and Immunity, 1990Co-Authors: Shlomo Rottem, K Groover, W H Habig, Maria Barile, M C HardegreeAbstract:The permeability properties of Mycoplasma gallisepticum cells treated with a purified preparation of Tetanolysin were investigated by determining the initial swelling rates of cells suspended in an isoosmotic solution of electrolytes or nonelectrolytes. The swelling, initiated by the Tetanolysin, depended on the Tetanolysin concentration and was markedly affected by the molecular size of the various osmotic stabilizers utilized. Thus, the initial swelling rates in an isoosmotic solution of monosaccharides were much higher than those in isoosmotic solutions of di-, tri-, or tetrasaccharides. Cell swelling induced by Tetanolysin was much lower with energy-depleted M. gallisepticum cells, with arsenate-treated cells, or when the membrane potential (delta psi) was collapsed by valinomycin (10 microM) plus KCl (100 mM). Swelling was not affected by the proton-conducting ionophore carbonyl cyanide-m-chlorophenylhydrazone (1 to 10 microM) or by nigericin (5 microM). These results support the concept that the damage induced by Tetanolysin is due to the formation of water-filled pores within the membranes of energized M. gallisepticum cells. Such pores allow the diffusion of hydrophilic molecules into the cells and may vary in size, depending on the Tetanolysin concentration utilized.