The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Neil J Oldham - One of the best experts on this subject based on the ideXlab platform.
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rapid and sensitive analysis of azadirachtin and related triterpenoids from neem azadirachta indica by high performance liquid chromatography atmospheric pressure chemical ionization mass spectrometry
Journal of Chromatography A, 2000Co-Authors: Otmar Schaaf, Andrew P Jarvis, S A Van Der Esch, Germina Giagnacovo, Neil J OldhamAbstract:Abstract Based on reversed-phase high-performance liquid chromatography (RP-HPLC) and atmospheric pressure chemical ionization (APCI) mass spectrometry, a HPLC–MS method was developed to permit the rapid qualitative and quantitative analysis of azadirachtin and related Tetranortriterpenoids from seeds and tissue cultures of Neem (Azadirachta indica). APCI+ standard scanning mass spectra of the major Neem triterpenoids were recorded and utilized to select suitable ions for selected ion monitoring (SIM). Transitions for selective reaction monitoring (SRM) were based on MS–MS experiments. Using SIM, major Neem triterpenoids were detected in callus culture material and seed kernels of A. indica. The limit of detection for azadirachtin in extract samples (∼1 ng ml−1 or 10 pg in SIM mode) was determined to be (with respect to injected absolute amounts) approximately 1000-times lower than values quoted in the literature for existing HPLC methods (∼200 ng ml−1 or 10 ng). In addition to high sensitivity, the HPLC–MS method is able to tolerate minimal sample preparation and purification, dramatically reducing total analysis time.
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rapid and sensitive analysis of azadirachtin and related triterpenoids from neem azadirachta indica by high performance liquid chromatography atmospheric pressure chemical ionization mass spectrometry
Journal of Chromatography A, 2000Co-Authors: Otmar Schaaf, Andrew P Jarvis, S A Van Der Esch, Germina Giagnacovo, Neil J OldhamAbstract:Based on reversed-phase high-performance liquid chromatography (RP-HPLC) and atmospheric pressure chemical ionization (APCI) mass spectrometry, a HPLC-MS method was developed to permit the rapid qualitative and quantitative analysis of azadirachtin and related Tetranortriterpenoids from seeds and tissue cultures of Neem (Azadirachta indica). APCI+ standard scanning mass spectra of the major Neem triterpenoids were recorded and utilized to select suitable ions for selected ion monitoring (SIM). Transitions for selective reaction monitoring (SRM) were based on MS-MS experiments. Using SIM, major Neem triterpenoids were detected in callus culture material and seed kernels of A. indica. The limit of detection for azadirachtin in extract samples (approximately 1 ng ml(-1) or 10 pg in SIM mode) was determined to be (with respect to injected absolute amounts) approximately 1000-times lower than values quoted in the literature for existing HPLC methods (approximately 200 ng ml(-1) or 10 ng). In addition to high sensitivity, the HPLC-MS method is able to tolerate minimal sample preparation and purification, dramatically reducing total analysis time.
Andrew P Jarvis - One of the best experts on this subject based on the ideXlab platform.
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rapid and sensitive analysis of azadirachtin and related triterpenoids from neem azadirachta indica by high performance liquid chromatography atmospheric pressure chemical ionization mass spectrometry
Journal of Chromatography A, 2000Co-Authors: Otmar Schaaf, Andrew P Jarvis, S A Van Der Esch, Germina Giagnacovo, Neil J OldhamAbstract:Based on reversed-phase high-performance liquid chromatography (RP-HPLC) and atmospheric pressure chemical ionization (APCI) mass spectrometry, a HPLC-MS method was developed to permit the rapid qualitative and quantitative analysis of azadirachtin and related Tetranortriterpenoids from seeds and tissue cultures of Neem (Azadirachta indica). APCI+ standard scanning mass spectra of the major Neem triterpenoids were recorded and utilized to select suitable ions for selected ion monitoring (SIM). Transitions for selective reaction monitoring (SRM) were based on MS-MS experiments. Using SIM, major Neem triterpenoids were detected in callus culture material and seed kernels of A. indica. The limit of detection for azadirachtin in extract samples (approximately 1 ng ml(-1) or 10 pg in SIM mode) was determined to be (with respect to injected absolute amounts) approximately 1000-times lower than values quoted in the literature for existing HPLC methods (approximately 200 ng ml(-1) or 10 ng). In addition to high sensitivity, the HPLC-MS method is able to tolerate minimal sample preparation and purification, dramatically reducing total analysis time.
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rapid and sensitive analysis of azadirachtin and related triterpenoids from neem azadirachta indica by high performance liquid chromatography atmospheric pressure chemical ionization mass spectrometry
Journal of Chromatography A, 2000Co-Authors: Otmar Schaaf, Andrew P Jarvis, S A Van Der Esch, Germina Giagnacovo, Neil J OldhamAbstract:Abstract Based on reversed-phase high-performance liquid chromatography (RP-HPLC) and atmospheric pressure chemical ionization (APCI) mass spectrometry, a HPLC–MS method was developed to permit the rapid qualitative and quantitative analysis of azadirachtin and related Tetranortriterpenoids from seeds and tissue cultures of Neem (Azadirachta indica). APCI+ standard scanning mass spectra of the major Neem triterpenoids were recorded and utilized to select suitable ions for selected ion monitoring (SIM). Transitions for selective reaction monitoring (SRM) were based on MS–MS experiments. Using SIM, major Neem triterpenoids were detected in callus culture material and seed kernels of A. indica. The limit of detection for azadirachtin in extract samples (∼1 ng ml−1 or 10 pg in SIM mode) was determined to be (with respect to injected absolute amounts) approximately 1000-times lower than values quoted in the literature for existing HPLC methods (∼200 ng ml−1 or 10 ng). In addition to high sensitivity, the HPLC–MS method is able to tolerate minimal sample preparation and purification, dramatically reducing total analysis time.
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rapid separation of triterpenoids from neem seed extracts
Phytochemical Analysis, 1999Co-Authors: Andrew P Jarvis, David E Morgan, Christine EdwardsAbstract:A dichloromethane extract of the triterpenoids from seeds of the Neem tree (Azadirachta indica) can be fractionated rapidly and with economic use of solvents using the Biotage™ flash chromatography system. The fractions can be analysed by thin layer chromatography (TLC), or quantitatively, by supercritical fluid chromatography or high performance liquid chromatography (HPLC), for the determination of 11 Tetranortriterpenoids, including the natural insecticide azadirachtin. After a second pass through the Biotage flash column, pure compounds, including azadirachtin and salannin can be obtained directly, although these compounds comprise less than 0.3% of the total mass of the seeds extracted and are present in a complex mixture of similar substances. Copyright © 1999 John Wiley & Sons, Ltd.
Otmar Schaaf - One of the best experts on this subject based on the ideXlab platform.
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rapid and sensitive analysis of azadirachtin and related triterpenoids from neem azadirachta indica by high performance liquid chromatography atmospheric pressure chemical ionization mass spectrometry
Journal of Chromatography A, 2000Co-Authors: Otmar Schaaf, Andrew P Jarvis, S A Van Der Esch, Germina Giagnacovo, Neil J OldhamAbstract:Abstract Based on reversed-phase high-performance liquid chromatography (RP-HPLC) and atmospheric pressure chemical ionization (APCI) mass spectrometry, a HPLC–MS method was developed to permit the rapid qualitative and quantitative analysis of azadirachtin and related Tetranortriterpenoids from seeds and tissue cultures of Neem (Azadirachta indica). APCI+ standard scanning mass spectra of the major Neem triterpenoids were recorded and utilized to select suitable ions for selected ion monitoring (SIM). Transitions for selective reaction monitoring (SRM) were based on MS–MS experiments. Using SIM, major Neem triterpenoids were detected in callus culture material and seed kernels of A. indica. The limit of detection for azadirachtin in extract samples (∼1 ng ml−1 or 10 pg in SIM mode) was determined to be (with respect to injected absolute amounts) approximately 1000-times lower than values quoted in the literature for existing HPLC methods (∼200 ng ml−1 or 10 ng). In addition to high sensitivity, the HPLC–MS method is able to tolerate minimal sample preparation and purification, dramatically reducing total analysis time.
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rapid and sensitive analysis of azadirachtin and related triterpenoids from neem azadirachta indica by high performance liquid chromatography atmospheric pressure chemical ionization mass spectrometry
Journal of Chromatography A, 2000Co-Authors: Otmar Schaaf, Andrew P Jarvis, S A Van Der Esch, Germina Giagnacovo, Neil J OldhamAbstract:Based on reversed-phase high-performance liquid chromatography (RP-HPLC) and atmospheric pressure chemical ionization (APCI) mass spectrometry, a HPLC-MS method was developed to permit the rapid qualitative and quantitative analysis of azadirachtin and related Tetranortriterpenoids from seeds and tissue cultures of Neem (Azadirachta indica). APCI+ standard scanning mass spectra of the major Neem triterpenoids were recorded and utilized to select suitable ions for selected ion monitoring (SIM). Transitions for selective reaction monitoring (SRM) were based on MS-MS experiments. Using SIM, major Neem triterpenoids were detected in callus culture material and seed kernels of A. indica. The limit of detection for azadirachtin in extract samples (approximately 1 ng ml(-1) or 10 pg in SIM mode) was determined to be (with respect to injected absolute amounts) approximately 1000-times lower than values quoted in the literature for existing HPLC methods (approximately 200 ng ml(-1) or 10 ng). In addition to high sensitivity, the HPLC-MS method is able to tolerate minimal sample preparation and purification, dramatically reducing total analysis time.
Germina Giagnacovo - One of the best experts on this subject based on the ideXlab platform.
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rapid and sensitive analysis of azadirachtin and related triterpenoids from neem azadirachta indica by high performance liquid chromatography atmospheric pressure chemical ionization mass spectrometry
Journal of Chromatography A, 2000Co-Authors: Otmar Schaaf, Andrew P Jarvis, S A Van Der Esch, Germina Giagnacovo, Neil J OldhamAbstract:Abstract Based on reversed-phase high-performance liquid chromatography (RP-HPLC) and atmospheric pressure chemical ionization (APCI) mass spectrometry, a HPLC–MS method was developed to permit the rapid qualitative and quantitative analysis of azadirachtin and related Tetranortriterpenoids from seeds and tissue cultures of Neem (Azadirachta indica). APCI+ standard scanning mass spectra of the major Neem triterpenoids were recorded and utilized to select suitable ions for selected ion monitoring (SIM). Transitions for selective reaction monitoring (SRM) were based on MS–MS experiments. Using SIM, major Neem triterpenoids were detected in callus culture material and seed kernels of A. indica. The limit of detection for azadirachtin in extract samples (∼1 ng ml−1 or 10 pg in SIM mode) was determined to be (with respect to injected absolute amounts) approximately 1000-times lower than values quoted in the literature for existing HPLC methods (∼200 ng ml−1 or 10 ng). In addition to high sensitivity, the HPLC–MS method is able to tolerate minimal sample preparation and purification, dramatically reducing total analysis time.
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rapid and sensitive analysis of azadirachtin and related triterpenoids from neem azadirachta indica by high performance liquid chromatography atmospheric pressure chemical ionization mass spectrometry
Journal of Chromatography A, 2000Co-Authors: Otmar Schaaf, Andrew P Jarvis, S A Van Der Esch, Germina Giagnacovo, Neil J OldhamAbstract:Based on reversed-phase high-performance liquid chromatography (RP-HPLC) and atmospheric pressure chemical ionization (APCI) mass spectrometry, a HPLC-MS method was developed to permit the rapid qualitative and quantitative analysis of azadirachtin and related Tetranortriterpenoids from seeds and tissue cultures of Neem (Azadirachta indica). APCI+ standard scanning mass spectra of the major Neem triterpenoids were recorded and utilized to select suitable ions for selected ion monitoring (SIM). Transitions for selective reaction monitoring (SRM) were based on MS-MS experiments. Using SIM, major Neem triterpenoids were detected in callus culture material and seed kernels of A. indica. The limit of detection for azadirachtin in extract samples (approximately 1 ng ml(-1) or 10 pg in SIM mode) was determined to be (with respect to injected absolute amounts) approximately 1000-times lower than values quoted in the literature for existing HPLC methods (approximately 200 ng ml(-1) or 10 ng). In addition to high sensitivity, the HPLC-MS method is able to tolerate minimal sample preparation and purification, dramatically reducing total analysis time.
S A Van Der Esch - One of the best experts on this subject based on the ideXlab platform.
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rapid and sensitive analysis of azadirachtin and related triterpenoids from neem azadirachta indica by high performance liquid chromatography atmospheric pressure chemical ionization mass spectrometry
Journal of Chromatography A, 2000Co-Authors: Otmar Schaaf, Andrew P Jarvis, S A Van Der Esch, Germina Giagnacovo, Neil J OldhamAbstract:Abstract Based on reversed-phase high-performance liquid chromatography (RP-HPLC) and atmospheric pressure chemical ionization (APCI) mass spectrometry, a HPLC–MS method was developed to permit the rapid qualitative and quantitative analysis of azadirachtin and related Tetranortriterpenoids from seeds and tissue cultures of Neem (Azadirachta indica). APCI+ standard scanning mass spectra of the major Neem triterpenoids were recorded and utilized to select suitable ions for selected ion monitoring (SIM). Transitions for selective reaction monitoring (SRM) were based on MS–MS experiments. Using SIM, major Neem triterpenoids were detected in callus culture material and seed kernels of A. indica. The limit of detection for azadirachtin in extract samples (∼1 ng ml−1 or 10 pg in SIM mode) was determined to be (with respect to injected absolute amounts) approximately 1000-times lower than values quoted in the literature for existing HPLC methods (∼200 ng ml−1 or 10 ng). In addition to high sensitivity, the HPLC–MS method is able to tolerate minimal sample preparation and purification, dramatically reducing total analysis time.
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rapid and sensitive analysis of azadirachtin and related triterpenoids from neem azadirachta indica by high performance liquid chromatography atmospheric pressure chemical ionization mass spectrometry
Journal of Chromatography A, 2000Co-Authors: Otmar Schaaf, Andrew P Jarvis, S A Van Der Esch, Germina Giagnacovo, Neil J OldhamAbstract:Based on reversed-phase high-performance liquid chromatography (RP-HPLC) and atmospheric pressure chemical ionization (APCI) mass spectrometry, a HPLC-MS method was developed to permit the rapid qualitative and quantitative analysis of azadirachtin and related Tetranortriterpenoids from seeds and tissue cultures of Neem (Azadirachta indica). APCI+ standard scanning mass spectra of the major Neem triterpenoids were recorded and utilized to select suitable ions for selected ion monitoring (SIM). Transitions for selective reaction monitoring (SRM) were based on MS-MS experiments. Using SIM, major Neem triterpenoids were detected in callus culture material and seed kernels of A. indica. The limit of detection for azadirachtin in extract samples (approximately 1 ng ml(-1) or 10 pg in SIM mode) was determined to be (with respect to injected absolute amounts) approximately 1000-times lower than values quoted in the literature for existing HPLC methods (approximately 200 ng ml(-1) or 10 ng). In addition to high sensitivity, the HPLC-MS method is able to tolerate minimal sample preparation and purification, dramatically reducing total analysis time.