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B.i. Van Tussenbroek - One of the best experts on this subject based on the ideXlab platform.
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Changes in trace metals in Thalassia Testudinum after hurricane impacts.
Marine pollution bulletin, 2011Co-Authors: T. Whelan, B.i. Van Tussenbroek, M.g. Barba SantosAbstract:Abstract Major hurricanes Emily and Wilma hit the Mexican Caribbean in 2005. Changes in trace metals in the seagrass Thalassia Testudinum prior to (May 2004, 2005) and following passage of these hurricanes (May, June 2006) were determined at four locations along a ∼130 km long stretch of coast. Before the hurricanes, essential metals were likely limiting and concentrations of potentially toxic Pb were high in a contaminated lagoon (27.5 μg g −1 ) and near submarine springs (6.10 μg g −1 ); the likely sources were inland sewage disposal or excessive boat traffic. After the hurricanes, Pb decreased to 2.0 μg g −1 in the contaminated lagoon probably through flushing. At the northern sites, essential Fe increased >2-fold (from 26.8 to 68.3 μg g −1 on average), possibly from remobilization of anoxic sediments or upwelling of deep seawater during Wilma. Thus, hurricanes can be beneficial to seagrass beds in flushing toxic metals and replenishing essential elements.
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Synchronized anthesis and predation on pollen in the marine angiosperm Thalassia Testudinum (Hydrocharitaceae)
Marine Ecology Progress Series, 2008Co-Authors: B.i. Van Tussenbroek, Jgr Wong, Judith Márquez-guzmánAbstract:Synchrony in the anthesis of male and female flowers of the hydrophilous dioecious marine angiosperm Thalassia Testudinum was studied by following flower buds of 64 staminate and 34 carpellate flowers in situ during night and day to observe the timing of flower opening. Anthesis of female flowers occurred throughout the day, with a slight peak between 15:00 and 17:00 h. The time lapse between initiation of anthesis and full opening of the flowers was ∼2 to 3 h. Anthesis in male flowers was highly synchronized, and all ripe primordia initiated anthesis within 1 h at dusk at ∼18:00 h, and pollen was released within 1 to 2 h. Male flowers in anthesis, or briefly after anthesis, were a targeted food source for herbivorous fish and >30% of the staminate flowers were consumed during our observations. The highly synchronized nocturnal pollen release is unusual for an abiotic pollinator, and we hypothesize that this may be a mechanism to ensure fertilization or, alternatively, may be a reponse to avoid pollen predation by fish.
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Effects of seagrass Thalassia Testudinum on sediment redox
Marine Ecology Progress Series, 2001Co-Authors: Susana Enríquez, B.i. Van Tussenbroek, Núria Marbà, Carlos M. Duarte, G. Reyes-zavalaAbstract:The redox conditions were compared in vegetated versus unvegetated sediments across a range of contrasting Thalassia Testudinum (sometimes mixed with Syringodium filiforme) meadows at Puerto Morelos Mexico reef lagoon. Moreover, the role of seagrass photosynthetic activity in affecting the redox conditions was tested in one of the meadows by experimentally reducing seagrass photosynthesis through shading. The seagrass rhizosphere extended 26 to 40 cm into the sediment, and accounted for 23 to 504 g DW m -2 of root material, mostly contributed by T. Testudinum. T. tes- tudinum placed 50% (i.e. centroidal depth) and 95% of its root biomass within 12.6 ± 0.58 and 54.4 ± 2.53 cm of the sediment surface, respectively; while S. filiforme placed 50 and 95% of its root biomass within 8.0 ± 0.87 and 34.7 ± 3.8 cm of the sediment surface, respectively. Vegetated sediments pre- sented 50% of positive redox potential anomaly (i.e. redox potential in vegetated sediments - redox potential in adjacent bare sediments), remarkably similar (t-test, p > 0.5) to depths to the centroidal depth of the seagrass roots in the sediments. The shading experiment conducted in situ for 5 d demonstrated that the positive redox anomaly found at depth in vegetated sediments was derived from seagrass photosynthetic activity. The sediments around seagrass rhizosphere in the shaded plots were progressively reduced to reach an average decline of the redox conditions by about 45 mV by Day 5. The results presented show that seagrasses contribute to modify sediment redox conditions around their rhizosphere.
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Thalassia Testudinum leaf dynamics in a Mexican Caribbean coral reef lagoon
Marine Biology, 1995Co-Authors: B.i. Van TussenbroekAbstract:Shoot density, leaf growth, initiation, biomass and primary production in Thalassia Testudinum (Banks ex Konig) were monitored at monthly intervals from August 1990 until January 1992 at three stations in the tropical coral reef of Puerto Morelos lagoon, Mexico. Leaf growth decreased with increasing leaf length, declining rapidly once the tips of leaves had started to decay; however, the leaves continued to grow until complete senescence. Maximum potential leaf age was>90 d. Leaf growth, biomass and primary production were highest at the station in the vicinity of mangrove discharges, intermediate at the nearshore fringe of the seagrass meadow, and lowest at the back-reef station. Leaf growth, leaf initiation, biomass and primary production were minimum in the winter months and maximum in the summer. Leaf growth and primary production were significantly correlated with water temperature or/and the hours of daylight. This is the first report of temperature-or/and hours of daylight-related seasonal variability in T. Testudinum production from the tropical Caribbean.
Idania Rodeiro - One of the best experts on this subject based on the ideXlab platform.
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Interaction of Thalassia Testudinum Metabolites with Cytochrome P450 Enzymes and Its Effects on Benzo(a)pyrene-Induced Mutagenicity
Marine drugs, 2020Co-Authors: Livan Delgado-roche, Rebeca Santes-palacios, José A. Herrera, Sandra Luz Hernandez, Mario Riera, Miguel D. Fernández, Fernando Mesta, Gabino Garrido, Idania Rodeiro, Jesús Javier Espinosa-aguirreAbstract:The aim of the present work was to evaluate the effects of Thalassia Testudinum hydroethanolic extract, its polyphenolic fraction and thalassiolin B on the activity of phase I metabolizing enzymes as well as their antimutagenic effects. Spectrofluorometric techniques were used to evaluate the effect of tested products on rat and human CYP1A and CYP2B activity. The antimutagenic effect of tested products was evaluated in benzo[a]pyrene (BP)-induced mutagenicity assay by an Ames test. Finally, the antimutagenic effect of Thalassia Testudinum (100 mg/kg) was assessed in BP-induced mutagenesis in mice. The tested products significantly (p < 0.05) inhibit rat CYP1A1 activity, acting as mixed-type inhibitors of rat CYP1A1 (Ki = 54.16 ± 9.09 μg/mL, 5.96 ± 1.55 μg/mL and 3.05 ± 0.89 μg/mL, respectively). Inhibition of human CYP1A1 was also observed (Ki = 197.1 ± 63.40 μg/mL and 203.10 ± 17.29 μg/mL for the polyphenolic fraction and for thalassiolin B, respectively). In addition, the evaluated products significantly inhibit (p < 0.05) BP-induced mutagenicity in vitro. Furthermore, oral doses of Thalassia Testudinum (100 mg/kg) significantly reduced (p < 0.05) the BP-induced micronuclei and oxidative damage, together with an increase of reduced glutathione, in mice. In summary, Thalassia Testudinum metabolites exhibit antigenotoxic activity mediated, at least, by the inhibition of CYP1A1-mediated BP biotransformation, arresting the oxidative and mutagenic damage. Thus, the metabolites of T. Testudinum may represent a potential source of chemopreventive compounds for the adjuvant therapy of cancer.
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Interaction of Thalassia Testudinum Metabolites with Cytochrome P450 Enzymes and Its Effects on Benzo(a)pyrene-Induced Mutagenicity
2020Co-Authors: Livan Delgado-roche, Rebeca Santes-palacios, José A. Herrera, Sandra Luz Hernandez, Mario Riera, Miguel D. Fernández, Fernando Mesta, Gabino Garrido, Idania Rodeiro, Jesús Javier Espinosa-aguirreAbstract:The aim of the present work was to evaluate the effects of Thalassia Testudinum hydroethanolic extract, its polyphenolic fraction, and thalassiolin B on the activity of phase I metabolizing enzymes as well as their antimutagenic effects. Spectrofluorometric techniques were used to evaluate the effect of tested products on rat and human CYP1A and CYP2B activity. The antimutagenic effect of tested products was evaluated in benzo[a]pyrene (BP)-induced mutagenicity assay by Ames test. Finally, the antimutagenic effect of Thalassia Testudinum (100 mg/kg) was assessed in a BP-induced mutagenesis in mice. The tested products significantly (p<0.05) inhibit rat CYP1A1 activity, acting as mixed-type inhibitors of rat CYP1A1 (Ki = 54.16±9.09 μg/mL, 5.96±1.55 μg/mL and 3.05±0.89 μg/mL, respectively). Inhibition of human CYP1A1 was also observed (Ki = 197.1±63.40 μg/mL and 203.10±17.29 μg/mL for the polyphenolic fraction and for thalassiolin B, respectively). In addition, the evaluated products significantly inhibit (p<0.05) benzo[a]pyrene (BP)-induced mutagenicity in vitro. Furthermore, oral doses of Thalassia Testudinum (100 mg/kg) significantly reduced (p<0.05) the BP-induced micronuclei and oxidative damage, together with an increase of glutathione, in mice. In summary, Thalassia Testudinum metabolites exhibit antigenotoxic activity mediated, at least, by the inhibition of CYP1A1-mediated BP biotransformation. Thus, the metabolites of T. Testudinum may represent a potential source of chemopreventive compounds for adjuvant therapy of cancer.
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assessment of the cytotoxic potential of an aqueous ethanolic extract from Thalassia Testudinum angiosperm marine grown in the caribbean sea
Journal of Pharmacy and Pharmacology, 2018Co-Authors: Idania Rodeiro, José A. Herrera, Mario Riera, Ivones Hernández, María Teresa Donato, Laia Tolosa, Kethia González, Yadira Ansoar, Maria Jose Gomezlechon, Wim Vanden BergheAbstract:OBJECTIVES Reported antioxidant, anti-inflammatory and neuroprotective properties for one aqueous-ethanolic extract from Thalassia Testudinum which grows in the Caribbean Sea compelled us to explore about extract cytotoxic effects. METHODS Cell viability was assayed on tumour (HepG2, PC12, Caco-2 and 4T1) and non-tumour (VERO, 3T3, CHO, MCDK and BHK2) cell lines. The extract effects upon primary cultures of rat and human hepatocytes and human lymphocytes were assayed. KEY FINDINGS The extract exhibited cytotoxicity against cancer cells compared to normal cells, and the IC50 values were 102 μg/ml for HepG2, 135 μg/ml for PC12, 165 μg/ml for Caco-2 and 129 μg/ml for 4T1 cells after 48 h, whereas IC50 could not be calculated for normal cells. Additional data from a high-content screening multiparametric assay indicated that after 24-h exposure, the extract (up to 100 μg/ml) induced death in HepG2 cells through oxidative stress-associated mechanism, DNA damage and hypercalcaemia. Comet assay corroborated extract-induced DNA damage. CONCLUSIONS Thalassia Testudinum extract is more cytotoxic and produced more DNA damage on human hepatoma cells than to other non-tumour cells. A possible mechanism is suggested for extract-induced cytotoxicity based on oxidative stress, nuclear damage and hypercalcaemia in HepG2 cells. T. Testudinum may be a source for antitumour agents.
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Assessment of the cytotoxic potential of an aqueous‐ethanolic extract from Thalassia Testudinum angiosperm marine grown in the Caribbean Sea
The Journal of pharmacy and pharmacology, 2018Co-Authors: Idania Rodeiro, José A. Herrera, Mario Riera, Ivones Hernández, María Teresa Donato, Laia Tolosa, Kethia González, Yadira Ansoar, María José Gómez-lechón, Wim Vanden BergheAbstract:OBJECTIVES Reported antioxidant, anti-inflammatory and neuroprotective properties for one aqueous-ethanolic extract from Thalassia Testudinum which grows in the Caribbean Sea compelled us to explore about extract cytotoxic effects. METHODS Cell viability was assayed on tumour (HepG2, PC12, Caco-2 and 4T1) and non-tumour (VERO, 3T3, CHO, MCDK and BHK2) cell lines. The extract effects upon primary cultures of rat and human hepatocytes and human lymphocytes were assayed. KEY FINDINGS The extract exhibited cytotoxicity against cancer cells compared to normal cells, and the IC50 values were 102 μg/ml for HepG2, 135 μg/ml for PC12, 165 μg/ml for Caco-2 and 129 μg/ml for 4T1 cells after 48 h, whereas IC50 could not be calculated for normal cells. Additional data from a high-content screening multiparametric assay indicated that after 24-h exposure, the extract (up to 100 μg/ml) induced death in HepG2 cells through oxidative stress-associated mechanism, DNA damage and hypercalcaemia. Comet assay corroborated extract-induced DNA damage. CONCLUSIONS Thalassia Testudinum extract is more cytotoxic and produced more DNA damage on human hepatoma cells than to other non-tumour cells. A possible mechanism is suggested for extract-induced cytotoxicity based on oxidative stress, nuclear damage and hypercalcaemia in HepG2 cells. T. Testudinum may be a source for antitumour agents.
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ABCG2/BCRP interaction with the sea grass Thalassia Testudinum.
Drug metabolism and personalized therapy, 2015Co-Authors: Verónica Miguel, Idania Rodeiro, Jon A. Otero, Borja Barrera, Julio G. Prieto, Gracia Merino, Ana I. ÁlvarezAbstract:BACKGROUND The aqueous ethanolic extract from leaves of the marine plant Thalassia Testudinum has shown antioxidant, cytoprotective, and neuroprotective properties. The chemical composition of this extract, rich in polyphenols, could interfere with active transport of drugs out of the cell and circumvent the phenomenon of multidrug resistance (MDR). The extract can act as an MDR modulator through its interaction with efflux transporters. The ABCG2/BCRP has been shown to confer MDR acting in tumor cells. METHODS To evaluate the interaction of ABCG2/BCRP with the extract, studies in cells overexpressing human BCRP transporter and its murine ortholog Bcrp1 were performed. RESULTS AND CONCLUSIONS T. Testudinum extract could be included as MDR modulator, as interaction with ABCG2/BCRP has been shown through flow cytometry and MTT assays. The cells overexpressing ABCG2/BCRP in the presence of the extract (25-150 μg/mL) decreased the survival rates of the anti-tumoral mitoxantrone. Our results support its inclusion as a possible MDR modulator against tumor cells that overexpress ABCG2/BCRP.
Brigitta I. Van Tussenbroek - One of the best experts on this subject based on the ideXlab platform.
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Range-wide population genetic structure of the Caribbean marine angiosperm Thalassia Testudinum.
Ecology and evolution, 2018Co-Authors: Kor-jent Van Dijk, Eric Bricker, Brigitta I. Van Tussenbroek, Michelle WaycottAbstract:Many marine species have widespread geographic ranges derived from their evolutionary and ecological history particularly their modes of dispersal. Seagrass (marine angiosperm) species have ranges that are unusually widespread, which is not unexpected following recent reviews of reproductive strategies demonstrating the potential for long-distance dispersal combined with longevity through clonality. An exemplar of these dual biological features is turtle grass (Thalassia Testudinum) which is an ecologically important species throughout the tropical Atlantic region. Turtle grass has been documented to have long-distance dispersal via floating fruits and also extreme clonality and longevity. We hypothesize that across its range, Thalassia Testudinum will have very limited regional population structure due to these characteristics and under typical models of population structure would expect to detect high levels of genetic connectivity. There are very few studies of range-wide genetic connectivity documented for seagrasses or other sessile marine species. This study presents a population genetic dataset that represents a geographic area exceeding 14,000 km2. Population genetic diversity was evaluated from 32 Thalassia Testudinum populations sampled across the Caribbean and Gulf of Mexico. Genotypes were based on nine microsatellites, and haplotypes were based on chloroplast DNA sequences. Very limited phylogeographic signal from cpDNA reduced the potential comparative analyses possible. Multiple analytical clustering approaches on population genetic data revealed two significant genetic partitions: (a) the Caribbean and (b) the Gulf of Mexico. Genetic diversity was high (HE = 0.641), and isolation by distance was significant; gene flow and migration estimates across the entire range were however modest, we suggest that the frequency of successful recruitment across the range is uncommon. Thalassia Testudinum maintains genetic diversity across its entire distribution range. The genetic split may be explained by genetic drift during recolonization from refugia following relatively recent reduction in available habitat such as the last glacial maxima.
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Clonal diversity and structure related to habitat of the marine angiosperm Thalassia Testudinum along the Atlantic coast of Mexico
Aquatic Botany, 2010Co-Authors: Jent Kornelis Van Dijk, Brigitta I. Van TussenbroekAbstract:The clonal structure of the tropical seagrass Thalassia Testudinum was studied at 16 sites along the Mexican Atlantic coast, situated in back-reef, shallow coastal and lagoon habitats. Thalassia Testudinum was highly clonal, with an overall average clonal richness (R) of 0.55. The largest genet found in this study extended throughout the sampling area (230 m), with an estimated max age almost reaching 600 years. Lagoons with higher nutrient availability reflected by nutrient content of leaves (mean leaf C:N ratio 11.4) and lower hydrodynamic regimes reflected by the percentage of fine sediments (on average 23%), sustained larger genets of T. Testudinum (mean of the largest genets over populations was 167.3 m) than the shallow coastal areas (C:N 12.3, 6.2% fine sediment, mean largest genet 10.3 m) and the more oligotrophic back-reefs (C:N 16.3, 2.7% fine sediment, mean largest genet 6.5 m). Population genetic analysis showed different levels of clonality, genotypic diversity and spatial genetic relatedness for this seagrass per habitat, with the lagoons presenting much lower levels of clonal diversity than the other two habitats.
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Polymorphic microsatellite markers for the Caribbeanseagrass Thalassia Testudinum Banks ex König
2007Co-Authors: Jent Kornelis Van Dijk, Brigitta I. Van Tussenbroek, Michelle Waycott, Joop OuborgAbstract:We isolated 14 polymorphic microsatellite loci for the western Atlantic tropical seagrass, Thalassia Testudinum, using two different enrichment procedures. Polymorphism was screened among samples from Mexico and Panama. Allelic diversity varied between three and 17 alleles per locus, and expected heterozygosity ranged from 0.271 to 0.859.
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Polymorphic microsatellite markers for the Caribbean seagrass Thalassia Testudinum Banks ex König
Molecular Ecology Notes, 2006Co-Authors: Jent Kornelis Van Dijk, Brigitta I. Van Tussenbroek, Michelle Waycott, Joop OuborgAbstract:We isolated 14 polymorphic microsatellite loci for the western Atlantic tropical seagrass, Thalassia Testudinum, using two different enrichment procedures. Polymorphism was screened among samples from Mexico and Panama. Allelic diversity varied between three and 17 alleles per locus, and expected heterozygosity ranged from 0.271 to 0.859.
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Static life-table analysis and demography of the foliar shoots of the Tropical seagrass Thalassia Testudinum
Bulletin of Marine Science, 2002Co-Authors: Brigitta I. Van TussenbroekAbstract:Dynamics of foliar shoots of the tropical seagrass Thalassia Testudinum (Banks ex Konig) at four sites in a Caribbean reef lagoon were evaluated by means of static life-table analysis. Prior to this analysis it was verified whether the instantaneous rate of increase of the populations approached zero (which was considered to be an indication of population stability), and whether shoot age could be determined by the Plastochrone Interval (PI). Mortality rates varied with age of the shoots, and were lower for younger shoots. Elasticity analysis showed that population growth was mainly accounted for by vegetative proliferation of first year old shoots. Within the lagoon, mortality and proliferation rates were lowest for a population in a wave-exposed back-reef area.
Judith Márquez-guzmán - One of the best experts on this subject based on the ideXlab platform.
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Synchronized anthesis and predation on pollen in the marine angiosperm Thalassia Testudinum (Hydrocharitaceae)
Marine Ecology Progress Series, 2008Co-Authors: B.i. Van Tussenbroek, Jgr Wong, Judith Márquez-guzmánAbstract:Synchrony in the anthesis of male and female flowers of the hydrophilous dioecious marine angiosperm Thalassia Testudinum was studied by following flower buds of 64 staminate and 34 carpellate flowers in situ during night and day to observe the timing of flower opening. Anthesis of female flowers occurred throughout the day, with a slight peak between 15:00 and 17:00 h. The time lapse between initiation of anthesis and full opening of the flowers was ∼2 to 3 h. Anthesis in male flowers was highly synchronized, and all ripe primordia initiated anthesis within 1 h at dusk at ∼18:00 h, and pollen was released within 1 to 2 h. Male flowers in anthesis, or briefly after anthesis, were a targeted food source for herbivorous fish and >30% of the staminate flowers were consumed during our observations. The highly synchronized nocturnal pollen release is unusual for an abiotic pollinator, and we hypothesize that this may be a mechanism to ensure fertilization or, alternatively, may be a reponse to avoid pollen predation by fish.
José A. Herrera - One of the best experts on this subject based on the ideXlab platform.
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Interaction of Thalassia Testudinum Metabolites with Cytochrome P450 Enzymes and Its Effects on Benzo(a)pyrene-Induced Mutagenicity
Marine drugs, 2020Co-Authors: Livan Delgado-roche, Rebeca Santes-palacios, José A. Herrera, Sandra Luz Hernandez, Mario Riera, Miguel D. Fernández, Fernando Mesta, Gabino Garrido, Idania Rodeiro, Jesús Javier Espinosa-aguirreAbstract:The aim of the present work was to evaluate the effects of Thalassia Testudinum hydroethanolic extract, its polyphenolic fraction and thalassiolin B on the activity of phase I metabolizing enzymes as well as their antimutagenic effects. Spectrofluorometric techniques were used to evaluate the effect of tested products on rat and human CYP1A and CYP2B activity. The antimutagenic effect of tested products was evaluated in benzo[a]pyrene (BP)-induced mutagenicity assay by an Ames test. Finally, the antimutagenic effect of Thalassia Testudinum (100 mg/kg) was assessed in BP-induced mutagenesis in mice. The tested products significantly (p < 0.05) inhibit rat CYP1A1 activity, acting as mixed-type inhibitors of rat CYP1A1 (Ki = 54.16 ± 9.09 μg/mL, 5.96 ± 1.55 μg/mL and 3.05 ± 0.89 μg/mL, respectively). Inhibition of human CYP1A1 was also observed (Ki = 197.1 ± 63.40 μg/mL and 203.10 ± 17.29 μg/mL for the polyphenolic fraction and for thalassiolin B, respectively). In addition, the evaluated products significantly inhibit (p < 0.05) BP-induced mutagenicity in vitro. Furthermore, oral doses of Thalassia Testudinum (100 mg/kg) significantly reduced (p < 0.05) the BP-induced micronuclei and oxidative damage, together with an increase of reduced glutathione, in mice. In summary, Thalassia Testudinum metabolites exhibit antigenotoxic activity mediated, at least, by the inhibition of CYP1A1-mediated BP biotransformation, arresting the oxidative and mutagenic damage. Thus, the metabolites of T. Testudinum may represent a potential source of chemopreventive compounds for the adjuvant therapy of cancer.
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Interaction of Thalassia Testudinum Metabolites with Cytochrome P450 Enzymes and Its Effects on Benzo(a)pyrene-Induced Mutagenicity
2020Co-Authors: Livan Delgado-roche, Rebeca Santes-palacios, José A. Herrera, Sandra Luz Hernandez, Mario Riera, Miguel D. Fernández, Fernando Mesta, Gabino Garrido, Idania Rodeiro, Jesús Javier Espinosa-aguirreAbstract:The aim of the present work was to evaluate the effects of Thalassia Testudinum hydroethanolic extract, its polyphenolic fraction, and thalassiolin B on the activity of phase I metabolizing enzymes as well as their antimutagenic effects. Spectrofluorometric techniques were used to evaluate the effect of tested products on rat and human CYP1A and CYP2B activity. The antimutagenic effect of tested products was evaluated in benzo[a]pyrene (BP)-induced mutagenicity assay by Ames test. Finally, the antimutagenic effect of Thalassia Testudinum (100 mg/kg) was assessed in a BP-induced mutagenesis in mice. The tested products significantly (p<0.05) inhibit rat CYP1A1 activity, acting as mixed-type inhibitors of rat CYP1A1 (Ki = 54.16±9.09 μg/mL, 5.96±1.55 μg/mL and 3.05±0.89 μg/mL, respectively). Inhibition of human CYP1A1 was also observed (Ki = 197.1±63.40 μg/mL and 203.10±17.29 μg/mL for the polyphenolic fraction and for thalassiolin B, respectively). In addition, the evaluated products significantly inhibit (p<0.05) benzo[a]pyrene (BP)-induced mutagenicity in vitro. Furthermore, oral doses of Thalassia Testudinum (100 mg/kg) significantly reduced (p<0.05) the BP-induced micronuclei and oxidative damage, together with an increase of glutathione, in mice. In summary, Thalassia Testudinum metabolites exhibit antigenotoxic activity mediated, at least, by the inhibition of CYP1A1-mediated BP biotransformation. Thus, the metabolites of T. Testudinum may represent a potential source of chemopreventive compounds for adjuvant therapy of cancer.
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assessment of the cytotoxic potential of an aqueous ethanolic extract from Thalassia Testudinum angiosperm marine grown in the caribbean sea
Journal of Pharmacy and Pharmacology, 2018Co-Authors: Idania Rodeiro, José A. Herrera, Mario Riera, Ivones Hernández, María Teresa Donato, Laia Tolosa, Kethia González, Yadira Ansoar, Maria Jose Gomezlechon, Wim Vanden BergheAbstract:OBJECTIVES Reported antioxidant, anti-inflammatory and neuroprotective properties for one aqueous-ethanolic extract from Thalassia Testudinum which grows in the Caribbean Sea compelled us to explore about extract cytotoxic effects. METHODS Cell viability was assayed on tumour (HepG2, PC12, Caco-2 and 4T1) and non-tumour (VERO, 3T3, CHO, MCDK and BHK2) cell lines. The extract effects upon primary cultures of rat and human hepatocytes and human lymphocytes were assayed. KEY FINDINGS The extract exhibited cytotoxicity against cancer cells compared to normal cells, and the IC50 values were 102 μg/ml for HepG2, 135 μg/ml for PC12, 165 μg/ml for Caco-2 and 129 μg/ml for 4T1 cells after 48 h, whereas IC50 could not be calculated for normal cells. Additional data from a high-content screening multiparametric assay indicated that after 24-h exposure, the extract (up to 100 μg/ml) induced death in HepG2 cells through oxidative stress-associated mechanism, DNA damage and hypercalcaemia. Comet assay corroborated extract-induced DNA damage. CONCLUSIONS Thalassia Testudinum extract is more cytotoxic and produced more DNA damage on human hepatoma cells than to other non-tumour cells. A possible mechanism is suggested for extract-induced cytotoxicity based on oxidative stress, nuclear damage and hypercalcaemia in HepG2 cells. T. Testudinum may be a source for antitumour agents.
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Assessment of the cytotoxic potential of an aqueous‐ethanolic extract from Thalassia Testudinum angiosperm marine grown in the Caribbean Sea
The Journal of pharmacy and pharmacology, 2018Co-Authors: Idania Rodeiro, José A. Herrera, Mario Riera, Ivones Hernández, María Teresa Donato, Laia Tolosa, Kethia González, Yadira Ansoar, María José Gómez-lechón, Wim Vanden BergheAbstract:OBJECTIVES Reported antioxidant, anti-inflammatory and neuroprotective properties for one aqueous-ethanolic extract from Thalassia Testudinum which grows in the Caribbean Sea compelled us to explore about extract cytotoxic effects. METHODS Cell viability was assayed on tumour (HepG2, PC12, Caco-2 and 4T1) and non-tumour (VERO, 3T3, CHO, MCDK and BHK2) cell lines. The extract effects upon primary cultures of rat and human hepatocytes and human lymphocytes were assayed. KEY FINDINGS The extract exhibited cytotoxicity against cancer cells compared to normal cells, and the IC50 values were 102 μg/ml for HepG2, 135 μg/ml for PC12, 165 μg/ml for Caco-2 and 129 μg/ml for 4T1 cells after 48 h, whereas IC50 could not be calculated for normal cells. Additional data from a high-content screening multiparametric assay indicated that after 24-h exposure, the extract (up to 100 μg/ml) induced death in HepG2 cells through oxidative stress-associated mechanism, DNA damage and hypercalcaemia. Comet assay corroborated extract-induced DNA damage. CONCLUSIONS Thalassia Testudinum extract is more cytotoxic and produced more DNA damage on human hepatoma cells than to other non-tumour cells. A possible mechanism is suggested for extract-induced cytotoxicity based on oxidative stress, nuclear damage and hypercalcaemia in HepG2 cells. T. Testudinum may be a source for antitumour agents.