The Experts below are selected from a list of 86310 Experts worldwide ranked by ideXlab platform
Wichien Yongmanitchai - One of the best experts on this subject based on the ideXlab platform.
-
selection and characterization of a newly isolated thermotolerant pichia kudriavzevii strain for ethanol production at high temperature from cassava starch hydrolysate
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2013Co-Authors: Napatchanok Yuangsaard, Wichien Yongmanitchai, Mumoru Yamada, Savitree LimtongAbstract:Pichia kudriavzevii DMKU 3-ET15 was isolated from traditional fermented pork sausage by an enrichment technique in a yeast extract peptone dextrose (YPD) broth, supplemented with 4 % (v/v) ethanol at 40 °C and selected based on its ethanol fermentation ability at 40 °C in YPD broth composed of 16 % glucose, and in a cassava starch hydrolysate medium composed of cassava starch hydrolysate adjusted to 16 % glucose. The strain produced ethanol from cassava starch hydrolysate at a high temperature up to 45 °C, but the optimal temperature for ethanol production was at 40 °C. Ethanol production by this strain using shaking flask cultivation was the highest in a medium containing cassava starch hydrolysate adjusted to 18 % glucose, 0.05 % (NH4)2SO4, 0.09 % yeast extract, 0.05 % KH2PO4, and 0.05 % MgSO4·7H2O, with a pH of 5.0 at 40 °C. The highest ethanol concentration reached 7.86 % (w/v) after 24 h, with productivity of 3.28 g/l/h and Yield of 85.4 % of the Theoretical Yield. At 42 °C, ethanol production by this strain became slightly lower, while at 45 °C only 3.82 % (w/v) of ethanol, 1.27 g/l/h productivity and 41.5 % of the Theoretical Yield were attained. In a study on ethanol production in a 2.5-l jar fermenter with an agitation speed of 300 rpm and an aeration rate of 0.1 vvm throughout the fermentation, P. kudriavzevii DMKU 3-ET15 Yielded a final ethanol concentration of 7.35 % (w/v) after 33 h, a productivity of 2.23 g/l/h and a Yield of 79.9 % of the Theoretical Yield.
-
production of fuel ethanol at high temperature from sugar cane juice by a newly isolated kluyveromyces marxianus
Bioresource Technology, 2007Co-Authors: Savitree Limtong, Chutima Sringiew, Wichien YongmanitchaiAbstract:Abstract Kluyveromyces marxianus DMKU 3-1042, isolated by an enrichment technique in a sugar cane juice medium supplemented with 4% (w/v) ethanol at 35 °C, produced high concentrations of ethanol at both 40 and 45 °C. Ethanol production by this strain in shaking flask cultivation in sugar cane juice media at 37 °C was highest in a medium containing 22% total sugars, 0.05% (NH 4 ) 2 SO 4 , 0.05% KH 2 PO 4 , and 0.15% MgSO 4 · 7H 2 O and having a pH of 5.0; the ethanol concentration reached 8.7% (w/v), productivity 1.45 g/l/h and Yield 77.5% of Theoretical Yield. At 40 °C, a maximal ethanol concentration of 6.78% (w/v), a productivity of 1.13 and a Yield 60.4% of Theoretical Yield were obtained from the same medium, except that the pH was adjusted to 5.5. In a study on ethanol production in a 5 l jar fermenter with an agitation speed of 300 rpm and an aeration rate of 0.2 vvm throughout the fermentation, K. marxianus DMKU 3-1042 Yielded a final ethanol concentration of 6.43% (w/v), a productivity of 1.3 g/l/h and a Yield of 57.1% of Theoretical Yield.
-
production of fuel ethanol at high temperature from sugar cane juice by a newly isolated kluyveromyces marxianus
Bioresource Technology, 2007Co-Authors: Savitree Limtong, Chutima Sringiew, Wichien YongmanitchaiAbstract:Kluyveromyces marxianus DMKU 3-1042, isolated by an enrichment technique in a sugar cane juice medium supplemented with 4% (w/v) ethanol at 35 degrees C, produced high concentrations of ethanol at both 40 and 45 degrees C. Ethanol production by this strain in shaking flask cultivation in sugar cane juice media at 37 degrees C was highest in a medium containing 22% total sugars, 0.05% (NH(4))(2)SO(4), 0.05% KH(2)PO(4), and 0.15% MgSO(4).7H(2)O and having a pH of 5.0; the ethanol concentration reached 8.7% (w/v), productivity 1.45 g/l/h and Yield 77.5% of Theoretical Yield. At 40 degrees C, a maximal ethanol concentration of 6.78% (w/v), a productivity of 1.13 and a Yield 60.4% of Theoretical Yield were obtained from the same medium, except that the pH was adjusted to 5.5. In a study on ethanol production in a 5l jar fermenter with an agitation speed of 300 rpm and an aeration rate of 0.2 vvm throughout the fermentation, K. marxianus DMKU 3-1042 Yielded a final ethanol concentration of 6.43% (w/v), a productivity of 1.3g/l/h and a Yield of 57.1% of Theoretical Yield.
Savitree Limtong - One of the best experts on this subject based on the ideXlab platform.
-
selection and characterization of a newly isolated thermotolerant pichia kudriavzevii strain for ethanol production at high temperature from cassava starch hydrolysate
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2013Co-Authors: Napatchanok Yuangsaard, Wichien Yongmanitchai, Mumoru Yamada, Savitree LimtongAbstract:Pichia kudriavzevii DMKU 3-ET15 was isolated from traditional fermented pork sausage by an enrichment technique in a yeast extract peptone dextrose (YPD) broth, supplemented with 4 % (v/v) ethanol at 40 °C and selected based on its ethanol fermentation ability at 40 °C in YPD broth composed of 16 % glucose, and in a cassava starch hydrolysate medium composed of cassava starch hydrolysate adjusted to 16 % glucose. The strain produced ethanol from cassava starch hydrolysate at a high temperature up to 45 °C, but the optimal temperature for ethanol production was at 40 °C. Ethanol production by this strain using shaking flask cultivation was the highest in a medium containing cassava starch hydrolysate adjusted to 18 % glucose, 0.05 % (NH4)2SO4, 0.09 % yeast extract, 0.05 % KH2PO4, and 0.05 % MgSO4·7H2O, with a pH of 5.0 at 40 °C. The highest ethanol concentration reached 7.86 % (w/v) after 24 h, with productivity of 3.28 g/l/h and Yield of 85.4 % of the Theoretical Yield. At 42 °C, ethanol production by this strain became slightly lower, while at 45 °C only 3.82 % (w/v) of ethanol, 1.27 g/l/h productivity and 41.5 % of the Theoretical Yield were attained. In a study on ethanol production in a 2.5-l jar fermenter with an agitation speed of 300 rpm and an aeration rate of 0.1 vvm throughout the fermentation, P. kudriavzevii DMKU 3-ET15 Yielded a final ethanol concentration of 7.35 % (w/v) after 33 h, a productivity of 2.23 g/l/h and a Yield of 79.9 % of the Theoretical Yield.
-
production of fuel ethanol at high temperature from sugar cane juice by a newly isolated kluyveromyces marxianus
Bioresource Technology, 2007Co-Authors: Savitree Limtong, Chutima Sringiew, Wichien YongmanitchaiAbstract:Abstract Kluyveromyces marxianus DMKU 3-1042, isolated by an enrichment technique in a sugar cane juice medium supplemented with 4% (w/v) ethanol at 35 °C, produced high concentrations of ethanol at both 40 and 45 °C. Ethanol production by this strain in shaking flask cultivation in sugar cane juice media at 37 °C was highest in a medium containing 22% total sugars, 0.05% (NH 4 ) 2 SO 4 , 0.05% KH 2 PO 4 , and 0.15% MgSO 4 · 7H 2 O and having a pH of 5.0; the ethanol concentration reached 8.7% (w/v), productivity 1.45 g/l/h and Yield 77.5% of Theoretical Yield. At 40 °C, a maximal ethanol concentration of 6.78% (w/v), a productivity of 1.13 and a Yield 60.4% of Theoretical Yield were obtained from the same medium, except that the pH was adjusted to 5.5. In a study on ethanol production in a 5 l jar fermenter with an agitation speed of 300 rpm and an aeration rate of 0.2 vvm throughout the fermentation, K. marxianus DMKU 3-1042 Yielded a final ethanol concentration of 6.43% (w/v), a productivity of 1.3 g/l/h and a Yield of 57.1% of Theoretical Yield.
-
production of fuel ethanol at high temperature from sugar cane juice by a newly isolated kluyveromyces marxianus
Bioresource Technology, 2007Co-Authors: Savitree Limtong, Chutima Sringiew, Wichien YongmanitchaiAbstract:Kluyveromyces marxianus DMKU 3-1042, isolated by an enrichment technique in a sugar cane juice medium supplemented with 4% (w/v) ethanol at 35 degrees C, produced high concentrations of ethanol at both 40 and 45 degrees C. Ethanol production by this strain in shaking flask cultivation in sugar cane juice media at 37 degrees C was highest in a medium containing 22% total sugars, 0.05% (NH(4))(2)SO(4), 0.05% KH(2)PO(4), and 0.15% MgSO(4).7H(2)O and having a pH of 5.0; the ethanol concentration reached 8.7% (w/v), productivity 1.45 g/l/h and Yield 77.5% of Theoretical Yield. At 40 degrees C, a maximal ethanol concentration of 6.78% (w/v), a productivity of 1.13 and a Yield 60.4% of Theoretical Yield were obtained from the same medium, except that the pH was adjusted to 5.5. In a study on ethanol production in a 5l jar fermenter with an agitation speed of 300 rpm and an aeration rate of 0.2 vvm throughout the fermentation, K. marxianus DMKU 3-1042 Yielded a final ethanol concentration of 6.43% (w/v), a productivity of 1.3g/l/h and a Yield of 57.1% of Theoretical Yield.
Ken Tokuyasu - One of the best experts on this subject based on the ideXlab platform.
-
ethanol production by repeated batch simultaneous saccharification and fermentation ssf of alkali treated rice straw using immobilized saccharomyces cerevisiae cells
Bioresource Technology, 2012Co-Authors: Itsuki Watanabe, Riki Shiroma, Ken Tokuyasu, Naonori Miyata, Akira Ando, Toshihide NakamuraAbstract:Abstract Repeated-batch simultaneous saccharification and fermentation (SSF) of alkali-treated rice straw using immobilized yeast was developed to produce ethanol. Saccharomyces cerevisiae cells were immobilized by entrapping in photocrosslinkable resin beads, and we evaluated the possibility of its reuse and ethanol production ability. In batch SSF of 20% (w/w) rice straw, the ethanol Yields based on the glucan content of the immobilized cells were slightly low (76.9% of the Theoretical Yield) compared to free cells (85.2% of the Theoretical Yield). In repeated-batch SSF of 20% (w/w) rice straw, stable ethanol production of approx. 38 g L−1 and an ethanol Yield of 84.7% were obtained. The immobilizing carrier could be reused without disintegration or any negative effect on ethanol production ability.
-
bioethanol production from rice straw by a sequential use of saccharomyces cerevisiae and pichia stipitis with heat inactivation of saccharomyces cerevisiae cells prior to xylose fermentation
Journal of Bioscience and Bioengineering, 2011Co-Authors: Jeungyil Park, Riki Shiroma, Ken TokuyasuAbstract:In order to establish an efficient bioethanol production system from rice straw, a new strategy to ferment the mixture of glucose and xylose by a sequential application of Saccharomyces cerevisiae and Pichia stipitis was developed, in which heat inactivation of S. cerevisiae cells before addition of P. stipitis was employed. The results showed that heating at 50°C for 6h was sufficient to give high xylose fermentation efficiency. By application of the inactivation process, 85% of the Theoretical Yield was achieved in the fermentation of the synthetic medium. At the same time, the xylitol production was reduced by 42.4% of the control process. In the simultaneous saccharification and fermentation of the lime-pretreated and CO(2)-neutralized rice straw, the inactivation of S. cerevisiae cells enabled the full conversion of glucose and xylose within 80 h. Finally, 21.1g/l of ethanol was produced from 10% (w/w) of pretreated rice straw and the ethanol Yield of rice straw reached 72.5% of the Theoretical Yield. This process is expected to be useful for the ethanol production from lignocellulosic materials in the regions where large-scale application of recombinant microorganisms was restricted.
Jo Shu Chang - One of the best experts on this subject based on the ideXlab platform.
-
continuous cellulosic bioethanol co fermentation by immobilized zymomonas mobilis and suspended pichia stipitis in a two stage process
Applied Energy, 2020Co-Authors: Ferdian Wirawan, Chun Yen Chen, Jo Shu Chang, Chieh Lun Cheng, Shao Yuan Leu, Duujong LeeAbstract:Abstract Bioethanol produced from lignocellulosic materials has been considered as one of the most promising fuels to replace fossil fuels. Immobilized yeasts or bacteria have been frequently used in continuous system due to its feasibility for repeated use with high biomass retention during the continuous process. In this study, continuous SHcF (separate hydrolysis and co-fermentation) and SScF (simultaneous saccharification and co-fermentation) were evaluated for ethanol production from alkaline pretreated sugarcane bagasse using Zymomonas mobilis (PVA immobilized cells) and Pichia stipitis (suspended cells). In SHcF fermentation, the ethanol Yield and productivity of 0.36 g ethanol/g cellulose (corresponding to 70.65% of Theoretical Yield) and 1.868 g/L/h were achieved. In contrast, SScF system resulted in an ethanol Yield of 0.414 g ethanol/g cellulose (corresponding to 81.17% of Theoretical Yield) and ethanol productivity of 0.705 g/L/h. The performance of the two systems are compared and discussed.
-
using recombinant cyanobacterium synechococcus elongatus with increased carbohydrate productivity as feedstock for bioethanol production via separate hydrolysis and fermentation process
Bioresource Technology, 2015Co-Authors: Te Jin Chow, Tsung Yu Tsai, Hsiang Hui Chou, Tse Min Lee, Jo Shu ChangAbstract:In this work, a recombinant cyanobacterium strain with increased photosynthesis rate, cell growth and carbohydrate production efficiency was genetically engineered by co-expressing ictB, ecaA, and acsAB (encoded for bacterial cellulose) in Synechococcus elongatus PCC7942. The resulting cyanobacterial biomass could be effectively hydrolyzed with dilute acid (2% sulfuric acid), achieving a nearly 90% glucose recovery at a biomass concentration of 80 g/L. Bioethanol can be produced from fermenting the acidic hydrolysate of S. elongatus PCC7942 via separate hydrolysis and fermentation (SHF) process at a concentration of 7.2 g/L and with a 91% Theoretical Yield.
-
bioethanol production using carbohydrate rich microalgae biomass as feedstock
Bioresource Technology, 2013Co-Authors: Shih-hsin Ho, Chun Yen Chen, Akihiko Kondo, Shu Wen Huang, Tomohisa Hasunuma, Jo Shu ChangAbstract:Abstract This study aimed to evaluate the potential of using a carbohydrate-rich microalga Chlorella vulgaris FSP-E as feedstock for bioethanol production via various hydrolysis strategies and fermentation processes. Enzymatic hydrolysis of C. vulgaris FSP-E biomass (containing 51% carbohydrate per dry weight) gave a glucose Yield of 90.4% (or 0.461 g (g biomass)−1). The SHF and SSF processes converted the enzymatic microalgae hydrolysate into ethanol with a 79.9% and 92.3% Theoretical Yield, respectively. Dilute acidic hydrolysis with 1% sulfuric acid was also very effective in saccharifying C. vulgaris FSP-E biomass, achieving a glucose Yield of nearly 93.6% from the microalgal carbohydrates at a starting biomass concentration of 50 g L−1. Using the acidic hydrolysate of C. vulgaris FSP-E biomass as feedstock, the SHF process produced ethanol at a concentration of 11.7 g L−1 and an 87.6% Theoretical Yield. These findings indicate the feasibility of using carbohydrate-producing microalgae as feedstock for fermentative bioethanol production.
Duujong Lee - One of the best experts on this subject based on the ideXlab platform.
-
continuous cellulosic bioethanol co fermentation by immobilized zymomonas mobilis and suspended pichia stipitis in a two stage process
Applied Energy, 2020Co-Authors: Ferdian Wirawan, Chun Yen Chen, Jo Shu Chang, Chieh Lun Cheng, Shao Yuan Leu, Duujong LeeAbstract:Abstract Bioethanol produced from lignocellulosic materials has been considered as one of the most promising fuels to replace fossil fuels. Immobilized yeasts or bacteria have been frequently used in continuous system due to its feasibility for repeated use with high biomass retention during the continuous process. In this study, continuous SHcF (separate hydrolysis and co-fermentation) and SScF (simultaneous saccharification and co-fermentation) were evaluated for ethanol production from alkaline pretreated sugarcane bagasse using Zymomonas mobilis (PVA immobilized cells) and Pichia stipitis (suspended cells). In SHcF fermentation, the ethanol Yield and productivity of 0.36 g ethanol/g cellulose (corresponding to 70.65% of Theoretical Yield) and 1.868 g/L/h were achieved. In contrast, SScF system resulted in an ethanol Yield of 0.414 g ethanol/g cellulose (corresponding to 81.17% of Theoretical Yield) and ethanol productivity of 0.705 g/L/h. The performance of the two systems are compared and discussed.
