The Experts below are selected from a list of 102 Experts worldwide ranked by ideXlab platform

Kimberly P Buehner - One of the best experts on this subject based on the ideXlab platform.

  • prevalence of Thermoduric Bacteria and spores in nonfat dry milk powders of midwest origin
    Journal of Dairy Science, 2015
    Co-Authors: Kimberly P Buehner, Sanjeev Anand, Gemechis D Djira
    Abstract:

    Samples of nonfat dry milk powder were analyzed for the presence of heat-resistant Bacteria. The samples were collected from Midwest manufacturing companies and were evaluated for the presence of spores, Thermoduric Bacteria, and the total Bacterial count. Three companies were included in this study, and results showed differences between each of the companies in the heat-resistant microbial groups tested. Company 3 had the highest levels of total spores and Thermoduric Bacteria: 3.6 ± 0.14 and 3.5 ± 0.13 log cfu/g, respectively. Interestingly, this company did not have the highest total Bacterial count but rather the second lowest total Bacterial count for the group, perhaps because of the higher proportion of thermophiles present in the powders from this company. The average level of total Bacterial counts was 2.57 ± 0.07 log cfu/g. Isolates obtained from the samples were identified by mass spectrometry, and all of the companies showed Bacillus licheniformis as the most prevalent Bacterial species identified.

  • corrigendum to prevalence of Thermoduric Bacteria and spores on 10 midwest dairy farms 1
    Journal of Dairy Science, 2014
    Co-Authors: Kimberly P Buehner, Sanjeev Anand, Gemechis D Djira, Alvaro Garcia
    Abstract:

    Thermoduric Bacteria (TDB), including sporeformers and their spores, can be present in milk and dairy products even after pasteurization. They have the potential to adversely affect the quality and shelf life of products. The objectives of this study were to identify the origin and common species of heat-resistant Bacteria occurring during summer and winter on Midwest dairy farms. Bulk tank milk samples were taken from 10 dairy farms located along the South Dakota section of Interstate 29, with herd sizes ranging from 650 to 3,500 lactating dairy cows. Milk samples were profiled for the prevalence of TDB and spore counts (SC). Corn silage samples and swabs of the milking clusters were also taken at the dairies to further profile the potential sources of TDB and SC. The samples were taken 3 times during 2 seasons [winter (January–March) and summer (June–August)] to track seasonal changes in the farm Bacterial flora. During winter, the average TDB counts in bulk tank milk were 1.83 log compared with 1.89 log TDB counts in the summer. The SC was 0.85 log in the winter, which was about half the 1.37 log SC present in the summer season. Corn silage sampled in winter contained 4.09 log TDB count compared with an increased 5.85 log TDB count during summer sampling. Concentrations of SC in corn silage reached an average of 3.60 log in winter compared with 6.33 log for summer. The seasonal effect was evident with an increase in summer counts across the board for TDB and SC, both in the feed and bulk tank milk samples. Bacillus licheniformis was the predominant species identified in 62.4% of winter (85 total) and 49.4% of summer (83 total) samples. Bacillus subtilis made up 9.4% of the remaining winter isolates, followed by Bacillus sonorensis at 8.2%. Conversely, B. sonorensis made up 12% of the summer isolates followed by Bacillus pumilus at 10.8%. Bacillus licheniformis is a ubiquitous microbe and was isolated from both TDB and sporeformer categories in all 3 sample types. There were larger increases in SC than TDB, indicating that summer temperatures and conditions may favor proliferation of sporeforming Bacteria over that of TDB. In conclusion, samples from bulk tank milk, milking cluster swabs, and corn silage samples at each of the 10 sites indicated that B. licheniformis was the major contaminant species, regardless of season. In this experiment, corn silage was the major environmental source of both TDB and SC with higher concentrations in summer when compared with winter.

  • prevalence of Thermoduric Bacteria and spores on 10 midwest dairy farms
    Journal of Dairy Science, 2014
    Co-Authors: Kimberly P Buehner, Sanjeev Anand, Alvaro Garcia
    Abstract:

    Abstract Thermoduric Bacteria (TDB), including sporeformers and their spores, can be present in milk and dairy products even after pasteurization. They have the potential to adversely affect the quality and shelf life of products. The objectives of this study were to identify the origin and common species of heat-resistant Bacteria occurring during summer and winter on Midwest dairy farms. Bulk tank milk samples were taken from 10 dairy farms located along the South Dakota section of Interstate 29, with herd sizes ranging from 650 to 3,500 lactating dairy cows. Milk samples were profiled for the prevalence of TDB and spore counts (SC). Corn silage samples and swabs of the milking clusters were also taken at the dairies to further profile the potential sources of TDB and SC. The samples were taken 3 times during 2 seasons [winter (January–March) and summer (June–August)] to track seasonal changes in the farm Bacterial flora. During winter, the average TDB counts in bulk tank milk were 2.61 log compared with 2.76 log TDB counts in the summer. The SC was 1.08 log in the winter, which was half the 2.06 log SC present in the summer season. Corn silage sampled in winter contained a 7.57 log TDB count compared with an increased 10.77 log TDB count during summer sampling. Concentrations of SC in corn silage reached an average of 6.3 log in winter compared with 11.81 log for summer. The seasonal effect was evident with an increase in summer counts across the board for TDB and SC, both in the feed and bulk tank milk samples. Bacillus licheniformis was the predominant species identified in 62.4% of winter (85 total) and 49.4% of summer (83 total) samples. Bacillus subtilis made up 9.4% of the remaining winter isolates, followed by Bacillus sonorensis at 8.2%. Conversely, B. sonorensis made up 12% of the summer isolates followed by Bacillus pumilus at 10.8%. Bacillus licheniformis is a ubiquitous microbe and was isolated from both TDB and sporeformer categories in all 3 sample types. There were larger increases in SC than TDB, indicating that summer temperatures and conditions may favor proliferation of sporeforming Bacteria over that of TDB. In conclusion, samples from bulk tank milk, milking cluster swabs, and corn silage samples at each of the 10 sites indicated that B. licheniformis was the major contaminant species, regardless of season. In this experiment, corn silage was the major environmental source of both TDB and SC with higher concentrations in summer when compared with winter.

Alvaro Garcia - One of the best experts on this subject based on the ideXlab platform.

  • corrigendum to prevalence of Thermoduric Bacteria and spores on 10 midwest dairy farms 1
    Journal of Dairy Science, 2014
    Co-Authors: Kimberly P Buehner, Sanjeev Anand, Gemechis D Djira, Alvaro Garcia
    Abstract:

    Thermoduric Bacteria (TDB), including sporeformers and their spores, can be present in milk and dairy products even after pasteurization. They have the potential to adversely affect the quality and shelf life of products. The objectives of this study were to identify the origin and common species of heat-resistant Bacteria occurring during summer and winter on Midwest dairy farms. Bulk tank milk samples were taken from 10 dairy farms located along the South Dakota section of Interstate 29, with herd sizes ranging from 650 to 3,500 lactating dairy cows. Milk samples were profiled for the prevalence of TDB and spore counts (SC). Corn silage samples and swabs of the milking clusters were also taken at the dairies to further profile the potential sources of TDB and SC. The samples were taken 3 times during 2 seasons [winter (January–March) and summer (June–August)] to track seasonal changes in the farm Bacterial flora. During winter, the average TDB counts in bulk tank milk were 1.83 log compared with 1.89 log TDB counts in the summer. The SC was 0.85 log in the winter, which was about half the 1.37 log SC present in the summer season. Corn silage sampled in winter contained 4.09 log TDB count compared with an increased 5.85 log TDB count during summer sampling. Concentrations of SC in corn silage reached an average of 3.60 log in winter compared with 6.33 log for summer. The seasonal effect was evident with an increase in summer counts across the board for TDB and SC, both in the feed and bulk tank milk samples. Bacillus licheniformis was the predominant species identified in 62.4% of winter (85 total) and 49.4% of summer (83 total) samples. Bacillus subtilis made up 9.4% of the remaining winter isolates, followed by Bacillus sonorensis at 8.2%. Conversely, B. sonorensis made up 12% of the summer isolates followed by Bacillus pumilus at 10.8%. Bacillus licheniformis is a ubiquitous microbe and was isolated from both TDB and sporeformer categories in all 3 sample types. There were larger increases in SC than TDB, indicating that summer temperatures and conditions may favor proliferation of sporeforming Bacteria over that of TDB. In conclusion, samples from bulk tank milk, milking cluster swabs, and corn silage samples at each of the 10 sites indicated that B. licheniformis was the major contaminant species, regardless of season. In this experiment, corn silage was the major environmental source of both TDB and SC with higher concentrations in summer when compared with winter.

  • prevalence of Thermoduric Bacteria and spores on 10 midwest dairy farms
    Journal of Dairy Science, 2014
    Co-Authors: Kimberly P Buehner, Sanjeev Anand, Alvaro Garcia
    Abstract:

    Abstract Thermoduric Bacteria (TDB), including sporeformers and their spores, can be present in milk and dairy products even after pasteurization. They have the potential to adversely affect the quality and shelf life of products. The objectives of this study were to identify the origin and common species of heat-resistant Bacteria occurring during summer and winter on Midwest dairy farms. Bulk tank milk samples were taken from 10 dairy farms located along the South Dakota section of Interstate 29, with herd sizes ranging from 650 to 3,500 lactating dairy cows. Milk samples were profiled for the prevalence of TDB and spore counts (SC). Corn silage samples and swabs of the milking clusters were also taken at the dairies to further profile the potential sources of TDB and SC. The samples were taken 3 times during 2 seasons [winter (January–March) and summer (June–August)] to track seasonal changes in the farm Bacterial flora. During winter, the average TDB counts in bulk tank milk were 2.61 log compared with 2.76 log TDB counts in the summer. The SC was 1.08 log in the winter, which was half the 2.06 log SC present in the summer season. Corn silage sampled in winter contained a 7.57 log TDB count compared with an increased 10.77 log TDB count during summer sampling. Concentrations of SC in corn silage reached an average of 6.3 log in winter compared with 11.81 log for summer. The seasonal effect was evident with an increase in summer counts across the board for TDB and SC, both in the feed and bulk tank milk samples. Bacillus licheniformis was the predominant species identified in 62.4% of winter (85 total) and 49.4% of summer (83 total) samples. Bacillus subtilis made up 9.4% of the remaining winter isolates, followed by Bacillus sonorensis at 8.2%. Conversely, B. sonorensis made up 12% of the summer isolates followed by Bacillus pumilus at 10.8%. Bacillus licheniformis is a ubiquitous microbe and was isolated from both TDB and sporeformer categories in all 3 sample types. There were larger increases in SC than TDB, indicating that summer temperatures and conditions may favor proliferation of sporeforming Bacteria over that of TDB. In conclusion, samples from bulk tank milk, milking cluster swabs, and corn silage samples at each of the 10 sites indicated that B. licheniformis was the major contaminant species, regardless of season. In this experiment, corn silage was the major environmental source of both TDB and SC with higher concentrations in summer when compared with winter.

Sanjeev Anand - One of the best experts on this subject based on the ideXlab platform.

  • prevalence of Thermoduric Bacteria and spores in nonfat dry milk powders of midwest origin
    Journal of Dairy Science, 2015
    Co-Authors: Kimberly P Buehner, Sanjeev Anand, Gemechis D Djira
    Abstract:

    Samples of nonfat dry milk powder were analyzed for the presence of heat-resistant Bacteria. The samples were collected from Midwest manufacturing companies and were evaluated for the presence of spores, Thermoduric Bacteria, and the total Bacterial count. Three companies were included in this study, and results showed differences between each of the companies in the heat-resistant microbial groups tested. Company 3 had the highest levels of total spores and Thermoduric Bacteria: 3.6 ± 0.14 and 3.5 ± 0.13 log cfu/g, respectively. Interestingly, this company did not have the highest total Bacterial count but rather the second lowest total Bacterial count for the group, perhaps because of the higher proportion of thermophiles present in the powders from this company. The average level of total Bacterial counts was 2.57 ± 0.07 log cfu/g. Isolates obtained from the samples were identified by mass spectrometry, and all of the companies showed Bacillus licheniformis as the most prevalent Bacterial species identified.

  • corrigendum to prevalence of Thermoduric Bacteria and spores on 10 midwest dairy farms 1
    Journal of Dairy Science, 2014
    Co-Authors: Kimberly P Buehner, Sanjeev Anand, Gemechis D Djira, Alvaro Garcia
    Abstract:

    Thermoduric Bacteria (TDB), including sporeformers and their spores, can be present in milk and dairy products even after pasteurization. They have the potential to adversely affect the quality and shelf life of products. The objectives of this study were to identify the origin and common species of heat-resistant Bacteria occurring during summer and winter on Midwest dairy farms. Bulk tank milk samples were taken from 10 dairy farms located along the South Dakota section of Interstate 29, with herd sizes ranging from 650 to 3,500 lactating dairy cows. Milk samples were profiled for the prevalence of TDB and spore counts (SC). Corn silage samples and swabs of the milking clusters were also taken at the dairies to further profile the potential sources of TDB and SC. The samples were taken 3 times during 2 seasons [winter (January–March) and summer (June–August)] to track seasonal changes in the farm Bacterial flora. During winter, the average TDB counts in bulk tank milk were 1.83 log compared with 1.89 log TDB counts in the summer. The SC was 0.85 log in the winter, which was about half the 1.37 log SC present in the summer season. Corn silage sampled in winter contained 4.09 log TDB count compared with an increased 5.85 log TDB count during summer sampling. Concentrations of SC in corn silage reached an average of 3.60 log in winter compared with 6.33 log for summer. The seasonal effect was evident with an increase in summer counts across the board for TDB and SC, both in the feed and bulk tank milk samples. Bacillus licheniformis was the predominant species identified in 62.4% of winter (85 total) and 49.4% of summer (83 total) samples. Bacillus subtilis made up 9.4% of the remaining winter isolates, followed by Bacillus sonorensis at 8.2%. Conversely, B. sonorensis made up 12% of the summer isolates followed by Bacillus pumilus at 10.8%. Bacillus licheniformis is a ubiquitous microbe and was isolated from both TDB and sporeformer categories in all 3 sample types. There were larger increases in SC than TDB, indicating that summer temperatures and conditions may favor proliferation of sporeforming Bacteria over that of TDB. In conclusion, samples from bulk tank milk, milking cluster swabs, and corn silage samples at each of the 10 sites indicated that B. licheniformis was the major contaminant species, regardless of season. In this experiment, corn silage was the major environmental source of both TDB and SC with higher concentrations in summer when compared with winter.

  • prevalence of Thermoduric Bacteria and spores on 10 midwest dairy farms
    Journal of Dairy Science, 2014
    Co-Authors: Kimberly P Buehner, Sanjeev Anand, Alvaro Garcia
    Abstract:

    Abstract Thermoduric Bacteria (TDB), including sporeformers and their spores, can be present in milk and dairy products even after pasteurization. They have the potential to adversely affect the quality and shelf life of products. The objectives of this study were to identify the origin and common species of heat-resistant Bacteria occurring during summer and winter on Midwest dairy farms. Bulk tank milk samples were taken from 10 dairy farms located along the South Dakota section of Interstate 29, with herd sizes ranging from 650 to 3,500 lactating dairy cows. Milk samples were profiled for the prevalence of TDB and spore counts (SC). Corn silage samples and swabs of the milking clusters were also taken at the dairies to further profile the potential sources of TDB and SC. The samples were taken 3 times during 2 seasons [winter (January–March) and summer (June–August)] to track seasonal changes in the farm Bacterial flora. During winter, the average TDB counts in bulk tank milk were 2.61 log compared with 2.76 log TDB counts in the summer. The SC was 1.08 log in the winter, which was half the 2.06 log SC present in the summer season. Corn silage sampled in winter contained a 7.57 log TDB count compared with an increased 10.77 log TDB count during summer sampling. Concentrations of SC in corn silage reached an average of 6.3 log in winter compared with 11.81 log for summer. The seasonal effect was evident with an increase in summer counts across the board for TDB and SC, both in the feed and bulk tank milk samples. Bacillus licheniformis was the predominant species identified in 62.4% of winter (85 total) and 49.4% of summer (83 total) samples. Bacillus subtilis made up 9.4% of the remaining winter isolates, followed by Bacillus sonorensis at 8.2%. Conversely, B. sonorensis made up 12% of the summer isolates followed by Bacillus pumilus at 10.8%. Bacillus licheniformis is a ubiquitous microbe and was isolated from both TDB and sporeformer categories in all 3 sample types. There were larger increases in SC than TDB, indicating that summer temperatures and conditions may favor proliferation of sporeforming Bacteria over that of TDB. In conclusion, samples from bulk tank milk, milking cluster swabs, and corn silage samples at each of the 10 sites indicated that B. licheniformis was the major contaminant species, regardless of season. In this experiment, corn silage was the major environmental source of both TDB and SC with higher concentrations in summer when compared with winter.

Gemechis D Djira - One of the best experts on this subject based on the ideXlab platform.

  • prevalence of Thermoduric Bacteria and spores in nonfat dry milk powders of midwest origin
    Journal of Dairy Science, 2015
    Co-Authors: Kimberly P Buehner, Sanjeev Anand, Gemechis D Djira
    Abstract:

    Samples of nonfat dry milk powder were analyzed for the presence of heat-resistant Bacteria. The samples were collected from Midwest manufacturing companies and were evaluated for the presence of spores, Thermoduric Bacteria, and the total Bacterial count. Three companies were included in this study, and results showed differences between each of the companies in the heat-resistant microbial groups tested. Company 3 had the highest levels of total spores and Thermoduric Bacteria: 3.6 ± 0.14 and 3.5 ± 0.13 log cfu/g, respectively. Interestingly, this company did not have the highest total Bacterial count but rather the second lowest total Bacterial count for the group, perhaps because of the higher proportion of thermophiles present in the powders from this company. The average level of total Bacterial counts was 2.57 ± 0.07 log cfu/g. Isolates obtained from the samples were identified by mass spectrometry, and all of the companies showed Bacillus licheniformis as the most prevalent Bacterial species identified.

  • corrigendum to prevalence of Thermoduric Bacteria and spores on 10 midwest dairy farms 1
    Journal of Dairy Science, 2014
    Co-Authors: Kimberly P Buehner, Sanjeev Anand, Gemechis D Djira, Alvaro Garcia
    Abstract:

    Thermoduric Bacteria (TDB), including sporeformers and their spores, can be present in milk and dairy products even after pasteurization. They have the potential to adversely affect the quality and shelf life of products. The objectives of this study were to identify the origin and common species of heat-resistant Bacteria occurring during summer and winter on Midwest dairy farms. Bulk tank milk samples were taken from 10 dairy farms located along the South Dakota section of Interstate 29, with herd sizes ranging from 650 to 3,500 lactating dairy cows. Milk samples were profiled for the prevalence of TDB and spore counts (SC). Corn silage samples and swabs of the milking clusters were also taken at the dairies to further profile the potential sources of TDB and SC. The samples were taken 3 times during 2 seasons [winter (January–March) and summer (June–August)] to track seasonal changes in the farm Bacterial flora. During winter, the average TDB counts in bulk tank milk were 1.83 log compared with 1.89 log TDB counts in the summer. The SC was 0.85 log in the winter, which was about half the 1.37 log SC present in the summer season. Corn silage sampled in winter contained 4.09 log TDB count compared with an increased 5.85 log TDB count during summer sampling. Concentrations of SC in corn silage reached an average of 3.60 log in winter compared with 6.33 log for summer. The seasonal effect was evident with an increase in summer counts across the board for TDB and SC, both in the feed and bulk tank milk samples. Bacillus licheniformis was the predominant species identified in 62.4% of winter (85 total) and 49.4% of summer (83 total) samples. Bacillus subtilis made up 9.4% of the remaining winter isolates, followed by Bacillus sonorensis at 8.2%. Conversely, B. sonorensis made up 12% of the summer isolates followed by Bacillus pumilus at 10.8%. Bacillus licheniformis is a ubiquitous microbe and was isolated from both TDB and sporeformer categories in all 3 sample types. There were larger increases in SC than TDB, indicating that summer temperatures and conditions may favor proliferation of sporeforming Bacteria over that of TDB. In conclusion, samples from bulk tank milk, milking cluster swabs, and corn silage samples at each of the 10 sites indicated that B. licheniformis was the major contaminant species, regardless of season. In this experiment, corn silage was the major environmental source of both TDB and SC with higher concentrations in summer when compared with winter.

Vanerli Beloti - One of the best experts on this subject based on the ideXlab platform.

  • effect of milk bactofugation on the counts and diversity of Thermoduric Bacteria
    Journal of Dairy Science, 2020
    Co-Authors: J C Ribeirojunior, Ronaldo Tamanini, Amauri Alcindo Alfieri, Vanerli Beloti
    Abstract:

    ABSTRACT The objective of this work was to determine the effect of milk bactofugation on the counts and microbial diversity of mesophilic (MT), psychrotrophic (PT), and thermophilic (TT) Thermoduric Bacteria and its potential as a technological method to remove spoilage microorganisms resistant to pasteurization. Different batches of raw milk from 69 dairy farms divided into sets in 3 bulk tanks (A, B, C) were evaluated at different times during the technological process. As the raw milk was preheated (∼55°C) immediately before bactofugation (10,000 × g), the effect of bactofugation was estimated by comparing the counts in raw, preheated, and bactofuged milk. This centrifugation was sufficient to reduce the isolation of 88% of the MT in preheated milk. For PT, it was possible to verify a reduction of 72.5% in batch C. The TT were not recovered at higher detection limits (