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Héctor F Terenzi - One of the best experts on this subject based on the ideXlab platform.

  • Biochemical characterisation of the trehalase of Thermophilic Fungi: an enzyme with mixed properties of neutral and acid trehalase.
    Biochimica et biophysica acta, 2005
    Co-Authors: Agda Karina B Lúcio-eterovic, João A Jorge, Maria De Lourdes T M Polizeli, Héctor F Terenzi
    Abstract:

    The trehalases from some Thermophilic Fungi, such as Humicola grisea, Scytalidium thermophilum, or Chaetomium thermophilum, possess mixed properties in comparison with those of the two main groups of trehalases: acid and neutral trehalases. Such as acid trehalases these enzymes are highly thermostable extracellular glycoproteins, which act at acidic pH. However, these enzymes are activated by calcium or manganese, and as a result inhibited by chelators and by ATP, properties typical of neutral trehalases. Here we extended the biochemical characterisation of these enzymes, by assaying their activity at acid and neutral pH. The acid activity (25-30% of total) was assayed in McIlvaine buffer at pH 4.5. Under these conditions the enzyme was neither activated by calcium nor inhibited by EDTA or ATP. The neutral activity was estimated in MES buffer at pH 6.5, after subtracting the activity resistant to EDTA inhibition. The neutral activity was activated by calcium and inhibited by ATP. On the other hand, the acid activity was more thermostable than the neutral activity, had a higher temperature optimum, exhibited a lower K(m), and different sensitivity to several ions and other substances. Apparently, these trehalases represent a new class of trehalases. More knowledge is needed about the molecular structure of this protein and its corresponding gene, to clarify the structural and evolutionary relationship of this trehalase to the conventional trehalases.

  • Biochemical characterisation of the trehalase of Thermophilic Fungi: an enzyme with mixed properties of neutral and acid trehalase.
    Biochimica et biophysica acta, 2005
    Co-Authors: Agda Karina B Lúcio-eterovic, João A Jorge, Maria De Lourdes T M Polizeli, Héctor F Terenzi
    Abstract:

    The trehalases from some Thermophilic Fungi, such as Humicola grisea, Scytalidium thermophilum, or Chaetomium thermophilum, possess mixed properties in comparison with those of the two main groups of trehalases: acid and neutral trehalases. Such as acid trehalases these enzymes are highly thermostable extracellular glycoproteins, which act at acidic pH. However, these enzymes are activated by calcium or manganese, and as a result inhibited by chelators and by ATP, properties typical of neutral trehalases. Here we extended the biochemical characterisation of these enzymes, by assaying their activity at acid and neutral pH. The acid activity (25-30% of total) was assayed in McIlvaine buffer at pH 4.5. Under these conditions the enzyme was neither activated by calcium nor inhibited by EDTA or ATP. The neutral activity was estimated in MES buffer at pH 6.5, after subtracting the activity resistant to EDTA inhibition. The neutral activity was activated by calcium and inhibited by ATP. On the other hand, the acid activity was more thermostable than the neutral activity, had a higher temperature optimum, exhibited a lower K(m), and different sensitivity to several ions and other substances. Apparently, these trehalases represent a new class of trehalases. More knowledge is needed about the molecular structure of this protein and its corresponding gene, to clarify the structural and evolutionary relationship of this trehalase to the conventional trehalases.

Agda Karina B Lúcio-eterovic - One of the best experts on this subject based on the ideXlab platform.

  • Biochemical characterisation of the trehalase of Thermophilic Fungi: an enzyme with mixed properties of neutral and acid trehalase.
    Biochimica et biophysica acta, 2005
    Co-Authors: Agda Karina B Lúcio-eterovic, João A Jorge, Maria De Lourdes T M Polizeli, Héctor F Terenzi
    Abstract:

    The trehalases from some Thermophilic Fungi, such as Humicola grisea, Scytalidium thermophilum, or Chaetomium thermophilum, possess mixed properties in comparison with those of the two main groups of trehalases: acid and neutral trehalases. Such as acid trehalases these enzymes are highly thermostable extracellular glycoproteins, which act at acidic pH. However, these enzymes are activated by calcium or manganese, and as a result inhibited by chelators and by ATP, properties typical of neutral trehalases. Here we extended the biochemical characterisation of these enzymes, by assaying their activity at acid and neutral pH. The acid activity (25-30% of total) was assayed in McIlvaine buffer at pH 4.5. Under these conditions the enzyme was neither activated by calcium nor inhibited by EDTA or ATP. The neutral activity was estimated in MES buffer at pH 6.5, after subtracting the activity resistant to EDTA inhibition. The neutral activity was activated by calcium and inhibited by ATP. On the other hand, the acid activity was more thermostable than the neutral activity, had a higher temperature optimum, exhibited a lower K(m), and different sensitivity to several ions and other substances. Apparently, these trehalases represent a new class of trehalases. More knowledge is needed about the molecular structure of this protein and its corresponding gene, to clarify the structural and evolutionary relationship of this trehalase to the conventional trehalases.

  • Biochemical characterisation of the trehalase of Thermophilic Fungi: an enzyme with mixed properties of neutral and acid trehalase.
    Biochimica et biophysica acta, 2005
    Co-Authors: Agda Karina B Lúcio-eterovic, João A Jorge, Maria De Lourdes T M Polizeli, Héctor F Terenzi
    Abstract:

    The trehalases from some Thermophilic Fungi, such as Humicola grisea, Scytalidium thermophilum, or Chaetomium thermophilum, possess mixed properties in comparison with those of the two main groups of trehalases: acid and neutral trehalases. Such as acid trehalases these enzymes are highly thermostable extracellular glycoproteins, which act at acidic pH. However, these enzymes are activated by calcium or manganese, and as a result inhibited by chelators and by ATP, properties typical of neutral trehalases. Here we extended the biochemical characterisation of these enzymes, by assaying their activity at acid and neutral pH. The acid activity (25-30% of total) was assayed in McIlvaine buffer at pH 4.5. Under these conditions the enzyme was neither activated by calcium nor inhibited by EDTA or ATP. The neutral activity was estimated in MES buffer at pH 6.5, after subtracting the activity resistant to EDTA inhibition. The neutral activity was activated by calcium and inhibited by ATP. On the other hand, the acid activity was more thermostable than the neutral activity, had a higher temperature optimum, exhibited a lower K(m), and different sensitivity to several ions and other substances. Apparently, these trehalases represent a new class of trehalases. More knowledge is needed about the molecular structure of this protein and its corresponding gene, to clarify the structural and evolutionary relationship of this trehalase to the conventional trehalases.

Annemieke Van Der Wal - One of the best experts on this subject based on the ideXlab platform.

  • Wood Degradation by Thermotolerant and Thermophilic Fungi for Sustainable Heat Production
    ACS Sustainable Chemistry & Engineering, 2016
    Co-Authors: Leire Caizán Juanarena, Annemiek Ter Heijne, Cees J.n. Buisman, Annemieke Van Der Wal
    Abstract:

    The use of renewable biomass for production of heat and electricity plays an important role in the circular economy. Degradation of wood biomass to produce heat is a clean and novel process proposed as an alternative to wood burning, and could be used for various heating applications. So far, wood degradation has mostly been studied at ambient temperatures. However, the process needs to occur at elevated temperatures (40–55 °C) to produce useable heat. Our objective was to study wood degradation at elevated temperatures for its potential application on heat production. Two (a thermotolerant and a Thermophilic) Fungi with different degradation strategies were chosen: lignin-degrading Phanerochaete chrysosporium and cellulose-degrading Chaetomium thermophilum. Each fungus was inoculated on nonsterile and sterile birch woodblocks to, respectively, study their wood degradation activity with and without natural biota (i.e., microorganisms naturally present in wood). The highest wood decay rates were found with...

Leo J L D Van Griensven - One of the best experts on this subject based on the ideXlab platform.

  • ecology of Thermophilic Fungi in mushroom compost with emphasis on scytalidium thermophilum and growth stimulation of agaricus bisporus mycelium
    Applied and Environmental Microbiology, 1994
    Co-Authors: Gerben Straatsma, Robert A Samson, Tineke W Olijnsma, Huub Op Den J M Camp, J P G Gerrits, Leo J L D Van Griensven
    Abstract:

    Twenty-two species of Thermophilic Fungi were isolated from mushroom compost. Scytalidium thermophilum was present in the compost ingredients, fresh straw, horse droppings, and drainage from compost and dominated the fungal biota of compost after preparation. Of 34 species of Thermophilic Fungi tested, 9 promoted mycelial growth of Agaricus bisporus on sterilized compost: Chaetomium thermophilum, an unidentified Chaetomium sp., Malbranchea sulfurea, Myriococcum thermophilum, S. thermophilum, Stilbella thermophila, Thielavia terrestris, and two unidentified basidiomycetes. These species will be considered for future experiments on inoculation and more controlled preparation of compost.

Andre Rodrigues - One of the best experts on this subject based on the ideXlab platform.

  • Ecology of Thermophilic Fungi
    Fungi in Extreme Environments: Ecological Role and Biotechnological Significance, 2019
    Co-Authors: Tássio Brito Oliveira, Andre Rodrigues
    Abstract:

    One of the most fascinating properties of microorganisms is their ability to adapt to extreme environments. While several extremes of physical and chemical factors may drive particular communities, temperature plays a major influence on the functions of biomolecules and the maintenance of their biological structures. The maximum temperature limit for eukaryotes has been recorded as 62 °C, and only a few species of the kingdom Fungi are known to be truly Thermophilic. Although distributed in taxonomically distinct lineages, Thermophilic Fungi constitute an ecologically well-defined group. In this review, we list 46 Thermophilic fungal species belonging to 23 genera. There are representatives from the phyla Mucoromycota, Ascomycota, and Basidiomycota. Thermophilic Fungi are common in habitats wherever organic matter decomposition takes place, leading to self-heating environments. Although Thermophilic Fungi have a great interest in biotechnology, knowledge of their ecology is still not clear. However, using the expertise from the applied perspective, one could perceive the roles of these Fungi in nature. Here, we discuss the various concepts of Thermophilic Fungi and how they developed through time in the literature, their classification, biology, biogeography, putative ecological roles, and mechanisms for adaptations to thermophily.

  • Thermophilic Fungi in the new age of fungal taxonomy
    Extremophiles, 2015
    Co-Authors: Tássio Brito Oliveira, Eleni Gomes, Andre Rodrigues
    Abstract:

    Thermophilic Fungi are of wide interest due to their potential to produce heat-tolerant enzymes for biotechnological processes. However, the taxonomy of such organisms remains obscure, especially given new developments in the nomenclature of Fungi. Here, we examine the taxonomy of the Thermophilic Fungi most commonly used in industry in light of the recent taxonomic changes following the adoption of the International Code of Nomenclature for Algae, Fungi and Plants and also based on the movement One Fungus = One Name. Despite the widespread use of these Fungi in applied research, several thermotolerant Fungi still remain classified as thermophiles. Furthermore, we found that while some Thermophilic Fungi have had their genomes sequenced, many taxa still do not have barcode sequences of reference strains available in public databases. This lack of basic information is a limiting factor for the species identification of Thermophilic Fungi and for metagenomic studies in this field. Based on next-generation sequencing, such studies generate large amounts of data, which may reveal new species of Thermophilic Fungi in different substrates (composting systems, geothermal areas, piles of plant material). As discussed in this study, there are intrinsic problems associated with this method, considering the actual state of the taxonomy of Thermophilic Fungi. To overcome such difficulties, the taxonomic classification of this group should move towards standardizing the commonly used species names in industry and to assess the possibility of including new systems for describing species based on environmental sequences.