The Experts below are selected from a list of 483 Experts worldwide ranked by ideXlab platform

Alexandra Scholze - One of the best experts on this subject based on the ideXlab platform.

  • Low expression of Thiosulfate Sulfurtransferase (rhodanese) predicts mortality in hemodialysis patients.
    Clinical Biochemistry, 2010
    Co-Authors: Katharina Krueger, Kathrin Koch, Anja Jühling, Martin Tepel, Alexandra Scholze
    Abstract:

    To test the hypothesis that impaired expression of the Thiosulfate Sulfurtransferase rhodanese is associated with oxidative stress and may predict mortality in hemodialysis patients. Sixty-two hemodialysis patients were investigated to determine protein and mRNA expression of rhodanese in monocytes. Whole cell reactive oxygen species and mitochondrial superoxide production were measured by fluorescence spectrophotometry. Compared to healthy subjects, hemodialysis patients showed significantly lower rhodanese mRNA and protein expression and significantly increased reactive oxygen species. Lower rhodanese protein expression was significantly associated with higher mitochondrial superoxide production. The hazard ratio for mortality in hemodialysis patients with rhodanese mRNA below compared to patients above the median was 2.22. Survival was shorter with rhodanese mRNA below compared to patients above the median. Impaired rhodanese expression is associated with increased whole cell reactive oxygen species as well as higher mitochondrial superoxide production and predicts mortality in hemodialysis patients. Copyright 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  • Low expression of Thiosulfate Sulfurtransferase (rhodanese) predicts mortality in hemodialysis patients.
    Clinical Biochemistry, 2009
    Co-Authors: Katharina Krueger, Kathrin Koch, Anja Jühling, Martin Tepel, Alexandra Scholze
    Abstract:

    Abstract Objectives To test the hypothesis that impaired expression of the Thiosulfate Sulfurtransferase rhodanese is associated with oxidative stress and may predict mortality in hemodialysis patients. Design and methods Sixty-two hemodialysis patients were investigated to determine protein and mRNA expression of rhodanese in monocytes. Whole cell reactive oxygen species and mitochondrial superoxide production were measured by fluorescence spectrophotometry. Results Compared to healthy subjects, hemodialysis patients showed significantly lower rhodanese mRNA and protein expression and significantly increased reactive oxygen species. Lower rhodanese protein expression was significantly associated with higher mitochondrial superoxide production. The hazard ratio for mortality in hemodialysis patients with rhodanese mRNA below compared to patients above the median was 2.22. Survival was shorter with rhodanese mRNA below compared to patients above the median. Conclusion Impaired rhodanese expression is associated with increased whole cell reactive oxygen species as well as higher mitochondrial superoxide production and predicts mortality in hemodialysis patients.

Katharina Krueger - One of the best experts on this subject based on the ideXlab platform.

  • Low expression of Thiosulfate Sulfurtransferase (rhodanese) predicts mortality in hemodialysis patients.
    Clinical Biochemistry, 2010
    Co-Authors: Katharina Krueger, Kathrin Koch, Anja Jühling, Martin Tepel, Alexandra Scholze
    Abstract:

    To test the hypothesis that impaired expression of the Thiosulfate Sulfurtransferase rhodanese is associated with oxidative stress and may predict mortality in hemodialysis patients. Sixty-two hemodialysis patients were investigated to determine protein and mRNA expression of rhodanese in monocytes. Whole cell reactive oxygen species and mitochondrial superoxide production were measured by fluorescence spectrophotometry. Compared to healthy subjects, hemodialysis patients showed significantly lower rhodanese mRNA and protein expression and significantly increased reactive oxygen species. Lower rhodanese protein expression was significantly associated with higher mitochondrial superoxide production. The hazard ratio for mortality in hemodialysis patients with rhodanese mRNA below compared to patients above the median was 2.22. Survival was shorter with rhodanese mRNA below compared to patients above the median. Impaired rhodanese expression is associated with increased whole cell reactive oxygen species as well as higher mitochondrial superoxide production and predicts mortality in hemodialysis patients. Copyright 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  • Low expression of Thiosulfate Sulfurtransferase (rhodanese) predicts mortality in hemodialysis patients.
    Clinical Biochemistry, 2009
    Co-Authors: Katharina Krueger, Kathrin Koch, Anja Jühling, Martin Tepel, Alexandra Scholze
    Abstract:

    Abstract Objectives To test the hypothesis that impaired expression of the Thiosulfate Sulfurtransferase rhodanese is associated with oxidative stress and may predict mortality in hemodialysis patients. Design and methods Sixty-two hemodialysis patients were investigated to determine protein and mRNA expression of rhodanese in monocytes. Whole cell reactive oxygen species and mitochondrial superoxide production were measured by fluorescence spectrophotometry. Results Compared to healthy subjects, hemodialysis patients showed significantly lower rhodanese mRNA and protein expression and significantly increased reactive oxygen species. Lower rhodanese protein expression was significantly associated with higher mitochondrial superoxide production. The hazard ratio for mortality in hemodialysis patients with rhodanese mRNA below compared to patients above the median was 2.22. Survival was shorter with rhodanese mRNA below compared to patients above the median. Conclusion Impaired rhodanese expression is associated with increased whole cell reactive oxygen species as well as higher mitochondrial superoxide production and predicts mortality in hemodialysis patients.

Rahul Bhattacharya - One of the best experts on this subject based on the ideXlab platform.

  • Dose and Time‐Dependent Effects of Cyanide on Thiosulfate Sulfurtransferase, 3‐Mercaptopyruvate Sulfurtransferase, and Cystathionine λ‐Lyase Activities
    Journal of Biochemical and Molecular Toxicology, 2013
    Co-Authors: Poonam Singh, Pooja Rao, Rahul Bhattacharya
    Abstract:

    We assessed the dose-dependent effect of potassium cyanide (KCN) on Thiosulfate Sulfurtransferase (TST), 3-mercaptopyruvate Sulfurtransferase (3-MPST), and cystathionine λ-lyase (CST) activities in mice. The time-dependent effect of 0.5 LD50 KCN on cyanide level and cytochrome c oxidase (CCO), TST, 3-MPST, and CST activities was also examined. Furthermore, TST, 3-MPST, and CST activities were measured in stored mice cadavers. Hepatic and renal TST activity increased by 0.5 LD50 KCN but diminished by ≥2.0 LD50. After 0.5 LD50 KCN, the elevated hepatic cyanide level was accompanied by increased TST, 3-MPST, and CST activities, and CCO inhibition. Elevated renal cyanide level was only accompanied by increased 3-MPST activity. No appreciable change in enzyme activities was observed in mice cadavers. The study concludes that high doses of cyanide exert saturating effects on its detoxification enzymes, indicating their exogenous use during cyanide poisoning. Also, these enzymes are not reliable markers of cyanide poisoning in autopsied samples.

  • dose and time dependent effects of cyanide on Thiosulfate Sulfurtransferase 3 mercaptopyruvate Sulfurtransferase and cystathionine λ lyase activities
    Journal of Biochemical and Molecular Toxicology, 2013
    Co-Authors: Poonam Singh, Pooja Rao, Rahul Bhattacharya
    Abstract:

    We assessed the dose-dependent effect of potassium cyanide (KCN) on Thiosulfate Sulfurtransferase (TST), 3-mercaptopyruvate Sulfurtransferase (3-MPST), and cystathionine λ-lyase (CST) activities in mice. The time-dependent effect of 0.5 LD50 KCN on cyanide level and cytochrome c oxidase (CCO), TST, 3-MPST, and CST activities was also examined. Furthermore, TST, 3-MPST, and CST activities were measured in stored mice cadavers. Hepatic and renal TST activity increased by 0.5 LD50 KCN but diminished by ≥2.0 LD50. After 0.5 LD50 KCN, the elevated hepatic cyanide level was accompanied by increased TST, 3-MPST, and CST activities, and CCO inhibition. Elevated renal cyanide level was only accompanied by increased 3-MPST activity. No appreciable change in enzyme activities was observed in mice cadavers. The study concludes that high doses of cyanide exert saturating effects on its detoxification enzymes, indicating their exogenous use during cyanide poisoning. Also, these enzymes are not reliable markers of cyanide poisoning in autopsied samples.

Kathrin Koch - One of the best experts on this subject based on the ideXlab platform.

  • Low expression of Thiosulfate Sulfurtransferase (rhodanese) predicts mortality in hemodialysis patients.
    Clinical Biochemistry, 2010
    Co-Authors: Katharina Krueger, Kathrin Koch, Anja Jühling, Martin Tepel, Alexandra Scholze
    Abstract:

    To test the hypothesis that impaired expression of the Thiosulfate Sulfurtransferase rhodanese is associated with oxidative stress and may predict mortality in hemodialysis patients. Sixty-two hemodialysis patients were investigated to determine protein and mRNA expression of rhodanese in monocytes. Whole cell reactive oxygen species and mitochondrial superoxide production were measured by fluorescence spectrophotometry. Compared to healthy subjects, hemodialysis patients showed significantly lower rhodanese mRNA and protein expression and significantly increased reactive oxygen species. Lower rhodanese protein expression was significantly associated with higher mitochondrial superoxide production. The hazard ratio for mortality in hemodialysis patients with rhodanese mRNA below compared to patients above the median was 2.22. Survival was shorter with rhodanese mRNA below compared to patients above the median. Impaired rhodanese expression is associated with increased whole cell reactive oxygen species as well as higher mitochondrial superoxide production and predicts mortality in hemodialysis patients. Copyright 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  • Low expression of Thiosulfate Sulfurtransferase (rhodanese) predicts mortality in hemodialysis patients.
    Clinical Biochemistry, 2009
    Co-Authors: Katharina Krueger, Kathrin Koch, Anja Jühling, Martin Tepel, Alexandra Scholze
    Abstract:

    Abstract Objectives To test the hypothesis that impaired expression of the Thiosulfate Sulfurtransferase rhodanese is associated with oxidative stress and may predict mortality in hemodialysis patients. Design and methods Sixty-two hemodialysis patients were investigated to determine protein and mRNA expression of rhodanese in monocytes. Whole cell reactive oxygen species and mitochondrial superoxide production were measured by fluorescence spectrophotometry. Results Compared to healthy subjects, hemodialysis patients showed significantly lower rhodanese mRNA and protein expression and significantly increased reactive oxygen species. Lower rhodanese protein expression was significantly associated with higher mitochondrial superoxide production. The hazard ratio for mortality in hemodialysis patients with rhodanese mRNA below compared to patients above the median was 2.22. Survival was shorter with rhodanese mRNA below compared to patients above the median. Conclusion Impaired rhodanese expression is associated with increased whole cell reactive oxygen species as well as higher mitochondrial superoxide production and predicts mortality in hemodialysis patients.

Anja Jühling - One of the best experts on this subject based on the ideXlab platform.

  • Low expression of Thiosulfate Sulfurtransferase (rhodanese) predicts mortality in hemodialysis patients.
    Clinical Biochemistry, 2010
    Co-Authors: Katharina Krueger, Kathrin Koch, Anja Jühling, Martin Tepel, Alexandra Scholze
    Abstract:

    To test the hypothesis that impaired expression of the Thiosulfate Sulfurtransferase rhodanese is associated with oxidative stress and may predict mortality in hemodialysis patients. Sixty-two hemodialysis patients were investigated to determine protein and mRNA expression of rhodanese in monocytes. Whole cell reactive oxygen species and mitochondrial superoxide production were measured by fluorescence spectrophotometry. Compared to healthy subjects, hemodialysis patients showed significantly lower rhodanese mRNA and protein expression and significantly increased reactive oxygen species. Lower rhodanese protein expression was significantly associated with higher mitochondrial superoxide production. The hazard ratio for mortality in hemodialysis patients with rhodanese mRNA below compared to patients above the median was 2.22. Survival was shorter with rhodanese mRNA below compared to patients above the median. Impaired rhodanese expression is associated with increased whole cell reactive oxygen species as well as higher mitochondrial superoxide production and predicts mortality in hemodialysis patients. Copyright 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  • Low expression of Thiosulfate Sulfurtransferase (rhodanese) predicts mortality in hemodialysis patients.
    Clinical Biochemistry, 2009
    Co-Authors: Katharina Krueger, Kathrin Koch, Anja Jühling, Martin Tepel, Alexandra Scholze
    Abstract:

    Abstract Objectives To test the hypothesis that impaired expression of the Thiosulfate Sulfurtransferase rhodanese is associated with oxidative stress and may predict mortality in hemodialysis patients. Design and methods Sixty-two hemodialysis patients were investigated to determine protein and mRNA expression of rhodanese in monocytes. Whole cell reactive oxygen species and mitochondrial superoxide production were measured by fluorescence spectrophotometry. Results Compared to healthy subjects, hemodialysis patients showed significantly lower rhodanese mRNA and protein expression and significantly increased reactive oxygen species. Lower rhodanese protein expression was significantly associated with higher mitochondrial superoxide production. The hazard ratio for mortality in hemodialysis patients with rhodanese mRNA below compared to patients above the median was 2.22. Survival was shorter with rhodanese mRNA below compared to patients above the median. Conclusion Impaired rhodanese expression is associated with increased whole cell reactive oxygen species as well as higher mitochondrial superoxide production and predicts mortality in hemodialysis patients.