The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Hideaki Kano - One of the best experts on this subject based on the ideXlab platform.
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multimodal nonlinear optical imaging of caenorhabditis elegans with multiplex coherent anti stokes raman scattering third harmonic Generation second harmonic Generation and two photon excitation fluorescence
Applied Physics Express, 2020Co-Authors: Shinichi Miyazaki, Philippe Leproux, Vincent Couderc, Yu Hayashi, Hideaki KanoAbstract:Multimodal nonlinear optical microscopy was applied to the nematode Caenorhabditis elegans (C. elegans). Transgenic worms expressing the yellow fluorescent protein (YFP) variant Venus underwent analyses. The lipid and protein distribution was clearly visualized using multiplex coherent anti-Stokes Raman scattering (CARS), second-harmonic Generation (SHG), Third-Harmonic Generation (THG), and two-photon excitation fluorescence (TPEF). The TPEF and SHG signals were found to overlap, which allowed the identification of the ventral nerve cord. These findings demonstrate the powerful potential of multimodal nonlinear optical microscopy to reveal C. elegans microstructures in vivo.
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label free tetra modal molecular imaging of living cells with cars shg thg and tsfg coherent anti stokes raman scattering second harmonic Generation third harmonic Generation and third order sum frequency Generation
Optics Express, 2012Co-Authors: Hiroki Segawa, Philippe Leproux, Vincent Couderc, Hideaki Kano, Masanari Okuno, Hiroo HamaguchiAbstract:We have developed a new multimodal molecular imaging system that combines CARS (coherent anti-Stokes Raman scattering), SHG (second harmonic Generation), THG (third harmonic Generation) and multiplex TSFG (third-order sum frequency Generation) using a subnanosecond white-light laser source. Molecular composition and their distribution in living cells are clearly visualized with different contrast enhancements through different mechanisms of CARS, SHG, THG and TSFG. A correlation image of CARS and TSF reveals that the TSF signal is generated predominantly from lipid droplets inside a cell as well as the peripheral cell wall.
Hiroo Hamaguchi - One of the best experts on this subject based on the ideXlab platform.
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label free tetra modal molecular imaging of living cells with cars shg thg and tsfg coherent anti stokes raman scattering second harmonic Generation third harmonic Generation and third order sum frequency Generation
Optics Express, 2012Co-Authors: Hiroki Segawa, Philippe Leproux, Vincent Couderc, Hideaki Kano, Masanari Okuno, Hiroo HamaguchiAbstract:We have developed a new multimodal molecular imaging system that combines CARS (coherent anti-Stokes Raman scattering), SHG (second harmonic Generation), THG (third harmonic Generation) and multiplex TSFG (third-order sum frequency Generation) using a subnanosecond white-light laser source. Molecular composition and their distribution in living cells are clearly visualized with different contrast enhancements through different mechanisms of CARS, SHG, THG and TSFG. A correlation image of CARS and TSF reveals that the TSF signal is generated predominantly from lipid droplets inside a cell as well as the peripheral cell wall.
Philippe Leproux - One of the best experts on this subject based on the ideXlab platform.
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multimodal nonlinear optical imaging of caenorhabditis elegans with multiplex coherent anti stokes raman scattering third harmonic Generation second harmonic Generation and two photon excitation fluorescence
Applied Physics Express, 2020Co-Authors: Shinichi Miyazaki, Philippe Leproux, Vincent Couderc, Yu Hayashi, Hideaki KanoAbstract:Multimodal nonlinear optical microscopy was applied to the nematode Caenorhabditis elegans (C. elegans). Transgenic worms expressing the yellow fluorescent protein (YFP) variant Venus underwent analyses. The lipid and protein distribution was clearly visualized using multiplex coherent anti-Stokes Raman scattering (CARS), second-harmonic Generation (SHG), Third-Harmonic Generation (THG), and two-photon excitation fluorescence (TPEF). The TPEF and SHG signals were found to overlap, which allowed the identification of the ventral nerve cord. These findings demonstrate the powerful potential of multimodal nonlinear optical microscopy to reveal C. elegans microstructures in vivo.
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label free tetra modal molecular imaging of living cells with cars shg thg and tsfg coherent anti stokes raman scattering second harmonic Generation third harmonic Generation and third order sum frequency Generation
Optics Express, 2012Co-Authors: Hiroki Segawa, Philippe Leproux, Vincent Couderc, Hideaki Kano, Masanari Okuno, Hiroo HamaguchiAbstract:We have developed a new multimodal molecular imaging system that combines CARS (coherent anti-Stokes Raman scattering), SHG (second harmonic Generation), THG (third harmonic Generation) and multiplex TSFG (third-order sum frequency Generation) using a subnanosecond white-light laser source. Molecular composition and their distribution in living cells are clearly visualized with different contrast enhancements through different mechanisms of CARS, SHG, THG and TSFG. A correlation image of CARS and TSF reveals that the TSF signal is generated predominantly from lipid droplets inside a cell as well as the peripheral cell wall.
Vincent Couderc - One of the best experts on this subject based on the ideXlab platform.
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multimodal nonlinear optical imaging of caenorhabditis elegans with multiplex coherent anti stokes raman scattering third harmonic Generation second harmonic Generation and two photon excitation fluorescence
Applied Physics Express, 2020Co-Authors: Shinichi Miyazaki, Philippe Leproux, Vincent Couderc, Yu Hayashi, Hideaki KanoAbstract:Multimodal nonlinear optical microscopy was applied to the nematode Caenorhabditis elegans (C. elegans). Transgenic worms expressing the yellow fluorescent protein (YFP) variant Venus underwent analyses. The lipid and protein distribution was clearly visualized using multiplex coherent anti-Stokes Raman scattering (CARS), second-harmonic Generation (SHG), Third-Harmonic Generation (THG), and two-photon excitation fluorescence (TPEF). The TPEF and SHG signals were found to overlap, which allowed the identification of the ventral nerve cord. These findings demonstrate the powerful potential of multimodal nonlinear optical microscopy to reveal C. elegans microstructures in vivo.
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label free tetra modal molecular imaging of living cells with cars shg thg and tsfg coherent anti stokes raman scattering second harmonic Generation third harmonic Generation and third order sum frequency Generation
Optics Express, 2012Co-Authors: Hiroki Segawa, Philippe Leproux, Vincent Couderc, Hideaki Kano, Masanari Okuno, Hiroo HamaguchiAbstract:We have developed a new multimodal molecular imaging system that combines CARS (coherent anti-Stokes Raman scattering), SHG (second harmonic Generation), THG (third harmonic Generation) and multiplex TSFG (third-order sum frequency Generation) using a subnanosecond white-light laser source. Molecular composition and their distribution in living cells are clearly visualized with different contrast enhancements through different mechanisms of CARS, SHG, THG and TSFG. A correlation image of CARS and TSF reveals that the TSF signal is generated predominantly from lipid droplets inside a cell as well as the peripheral cell wall.
S Tzortzakis - One of the best experts on this subject based on the ideXlab platform.
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filamentation induced third harmonic Generation in air via plasma enhanced third order susceptibility
Physical Review A, 2010Co-Authors: S Suntsov, Daryoush Abdollahpour, D G Papazoglou, S TzortzakisAbstract:We study, both experimentally and theoretically, the underlying physics of Third-Harmonic Generation in air by a filamented infrared femtosecond laser pulse propagating through a thin plasma channel. It is shown that the recently observed more than two-order-of-magnitude increase of the efficiency of Third-Harmonic Generation occurs due to the plasma-enhanced third-order susceptibility. An estimate of the effective value of this susceptibility is given.
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efficient third harmonic Generation through tailored ir femtosecond laser pulse filamentation in air
Quantum Electronics and Laser Science Conference, 2009Co-Authors: S Suntsov, Daryoush Abdollahpour, Dimitrios G Papazoglou, S TzortzakisAbstract:Third-Harmonic Generation during filamentation of IR femtosecond laser pulses in air experiences strong spatial reshaping and conversion enhancement when a thin plasma string created by another femtosecond pulse is introduced perpendicularly to the filament's path.
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efficient third harmonic Generation through tailored ir femtosecond laser pulse filamentation in air
Optics Express, 2009Co-Authors: S Suntsov, Daryoush Abdollahpour, Dimitrios G Papazoglou, S TzortzakisAbstract:The process of Third-Harmonic Generation during the filamentation of intense IR femtosecond laser pulses in air is investigated experimentally. It is shown that the introduction of a thin plasma string created by another femtosecond pulse, perpendicularly to the filament's path, dramatically reshapes the Third-Harmonic beam into a Bessel-like far-field distribution, while at the same time significantly enhances, up to 250 times, its conversion efficiency.