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Vadym F. Sagach - One of the best experts on this subject based on the ideXlab platform.

  • the role of the Thrombocyte Activating Factor in inhibiting na k atpase in different tissues after ischemia and reperfusion of the small intestine
    Ukrainskiĭ biokhimicheskiĭ zhurnal, 1996
    Co-Authors: G L Vavilova, O N Prokopenko, O N Kharlamova, M S Iaremenko, Vadym F. Sagach
    Abstract:

    Activity of transport Na+, K(+)-ATP-ase has been studied both in the ischemia-affected proximal section of the small intestine and in tissues which did not endure ischemia (the brain, renal cortex and the distal part of the small intestine) in dynamics 5, 30 and 60 min, after reperfusion of the ischemized small intestine of rats. It is shown that after 30 min-long ischemia of the small intestine, the Na+, K(+)-ATP-ase activity is inhibited in all the tissues mentioned. Maximal inhibition of the enzyme activity after 30 min long reperfusion is 74, 34, 94 and 58% for the brain, renal cortex, proximal and distal sections of the small intestine, respectively. It has been found that the blood serum from ischemized rats also exerts an inhibitory effect on the enzyme activity. Application of inhibitor of the Thrombocyte-Activating Factor (TAF) BN52021 has permitted demonstrating its role in the protecting effect under conditions of Na+, K(+)-ATP-ase inhibition in the post-Ischemic period and inhibitory effect of the blood serum from ischemized rats on the enzyme activity. A conclusion is made that the TAF participated in the inhibitory effect of Na+, K(+)-ATP-ase activity in different tissues after ischemia of the small intestine of rats.

  • The role of the Thrombocyte-Activating Factor in inhibiting Na+,K(+)-ATPase in different tissues after ischemia and reperfusion of the small intestine
    Ukrainskiĭ biokhimicheskiĭ zhurnal, 1996
    Co-Authors: Vavilova Gl, Prokopenko On, Kharlamova On, Iaremenko Ms, Vadym F. Sagach
    Abstract:

    Activity of transport Na+, K(+)-ATP-ase has been studied both in the ischemia-affected proximal section of the small intestine and in tissues which did not endure ischemia (the brain, renal cortex and the distal part of the small intestine) in dynamics 5, 30 and 60 min, after reperfusion of the ischemized small intestine of rats. It is shown that after 30 min-long ischemia of the small intestine, the Na+, K(+)-ATP-ase activity is inhibited in all the tissues mentioned. Maximal inhibition of the enzyme activity after 30 min long reperfusion is 74, 34, 94 and 58% for the brain, renal cortex, proximal and distal sections of the small intestine, respectively. It has been found that the blood serum from ischemized rats also exerts an inhibitory effect on the enzyme activity. Application of inhibitor of the Thrombocyte-Activating Factor (TAF) BN52021 has permitted demonstrating its role in the protecting effect under conditions of Na+, K(+)-ATP-ase inhibition in the post-Ischemic period and inhibitory effect of the blood serum from ischemized rats on the enzyme activity. A conclusion is made that the TAF participated in the inhibitory effect of Na+, K(+)-ATP-ase activity in different tissues after ischemia of the small intestine of rats.

Jj Emeis - One of the best experts on this subject based on the ideXlab platform.

  • The role of cyclic nucleotides in the release of tissue-type plasminogen activator and von Willebrand Factor
    Thrombosis and Haemostasis, 1993
    Co-Authors: N. Tranquille, Jj Emeis
    Abstract:

    The modulation of the induced acute release of tissue-type plasminogen activator (t-PA) and of von Willebrand Factor (vWF) by compounds affecting cyclic nucleotide levels was studied, using an isolated rat hindleg perfusion system. Platelet-Activating Factor (PAF; 5 nM) or bradykinin (0.8 μM) were used to induce release of t-PA and vWF. The guanylate cyclase activators sodium nitroprusside and atrial natriuretic Factor reduced the induced release of t-PA and vWF. Release was not affected by inhibiting nitric oxide production with N(G)-nitro-L-arginine. The effects of nitroprusside and atrial natriuretic Factor could not be reproduced by infusion of 8-bromo-cGMP. The adenylate cyclase activator forskolin had no effect on bradykinin-induced release of t-PA and vWF, reduced PAF-induced t-PA release, but potentiated PAF-induced vWF release. These modulatory effects were only partially mimicked by infusion of 8-bromo-cAMP. None of the compounds tested was able to induce the release of t-PA or of vWF in the absence of stimulation by bradykinin or platelet-Activating Factor. Cyclic nucleotides can thus modulate, but not induce, the acute release of t-PA and vWF from perfused rat hindlegs. Chemicals/CAS: 8 bromo cyclic AMP, 23583-48-4; adenylate cyclase, 9012-42-4; atrial natriuretic Factor, 85637-73-6; bradykinin, 58-82-2, 5979-11-3; forskolin, 66575-29-9; guanylate cyclase, 9054-75-5; n(g) nitroarginine, 2149-70-4; nitric oxide, 10102-43-9; nitroprusside sodium, 14402-89-2, 15078-28-1; Thrombocyte Activating Factor, 64176-80-3, 65154-06-5; tissue plasminogen activator, 105913-11-9; von Willebrand Factor, 109319-16-6; 8-Bromo Cyclic Adenosine Monophosphate, 23583-48-4; 8-bromocyclic GMP, 31356-94-2; Adenylate Cyclase, EC 4.6.1.1; Atrial Natriuretic Factor, 85637-73-6; Bradykinin, 58-82-2; Cyclic AMP, 60-92-4; Cyclic GMP, 7665-99-8; Forskolin, 66428-89-5; Guanylate Cyclase, EC 4.6.1.2; Nitroprusside, 15078-28-1; Platelet Activating Factor; Tissue Plasminogen Activator, EC 3.4.21.68; von Willebrand Factor

N. Tranquille - One of the best experts on this subject based on the ideXlab platform.

  • The role of cyclic nucleotides in the release of tissue-type plasminogen activator and von Willebrand Factor
    Thrombosis and Haemostasis, 1993
    Co-Authors: N. Tranquille, Jj Emeis
    Abstract:

    The modulation of the induced acute release of tissue-type plasminogen activator (t-PA) and of von Willebrand Factor (vWF) by compounds affecting cyclic nucleotide levels was studied, using an isolated rat hindleg perfusion system. Platelet-Activating Factor (PAF; 5 nM) or bradykinin (0.8 μM) were used to induce release of t-PA and vWF. The guanylate cyclase activators sodium nitroprusside and atrial natriuretic Factor reduced the induced release of t-PA and vWF. Release was not affected by inhibiting nitric oxide production with N(G)-nitro-L-arginine. The effects of nitroprusside and atrial natriuretic Factor could not be reproduced by infusion of 8-bromo-cGMP. The adenylate cyclase activator forskolin had no effect on bradykinin-induced release of t-PA and vWF, reduced PAF-induced t-PA release, but potentiated PAF-induced vWF release. These modulatory effects were only partially mimicked by infusion of 8-bromo-cAMP. None of the compounds tested was able to induce the release of t-PA or of vWF in the absence of stimulation by bradykinin or platelet-Activating Factor. Cyclic nucleotides can thus modulate, but not induce, the acute release of t-PA and vWF from perfused rat hindlegs. Chemicals/CAS: 8 bromo cyclic AMP, 23583-48-4; adenylate cyclase, 9012-42-4; atrial natriuretic Factor, 85637-73-6; bradykinin, 58-82-2, 5979-11-3; forskolin, 66575-29-9; guanylate cyclase, 9054-75-5; n(g) nitroarginine, 2149-70-4; nitric oxide, 10102-43-9; nitroprusside sodium, 14402-89-2, 15078-28-1; Thrombocyte Activating Factor, 64176-80-3, 65154-06-5; tissue plasminogen activator, 105913-11-9; von Willebrand Factor, 109319-16-6; 8-Bromo Cyclic Adenosine Monophosphate, 23583-48-4; 8-bromocyclic GMP, 31356-94-2; Adenylate Cyclase, EC 4.6.1.1; Atrial Natriuretic Factor, 85637-73-6; Bradykinin, 58-82-2; Cyclic AMP, 60-92-4; Cyclic GMP, 7665-99-8; Forskolin, 66428-89-5; Guanylate Cyclase, EC 4.6.1.2; Nitroprusside, 15078-28-1; Platelet Activating Factor; Tissue Plasminogen Activator, EC 3.4.21.68; von Willebrand Factor

Vavilova Gl - One of the best experts on this subject based on the ideXlab platform.

  • The role of the Thrombocyte-Activating Factor in inhibiting Na+,K(+)-ATPase in different tissues after ischemia and reperfusion of the small intestine
    Ukrainskiĭ biokhimicheskiĭ zhurnal, 1996
    Co-Authors: Vavilova Gl, Prokopenko On, Kharlamova On, Iaremenko Ms, Vadym F. Sagach
    Abstract:

    Activity of transport Na+, K(+)-ATP-ase has been studied both in the ischemia-affected proximal section of the small intestine and in tissues which did not endure ischemia (the brain, renal cortex and the distal part of the small intestine) in dynamics 5, 30 and 60 min, after reperfusion of the ischemized small intestine of rats. It is shown that after 30 min-long ischemia of the small intestine, the Na+, K(+)-ATP-ase activity is inhibited in all the tissues mentioned. Maximal inhibition of the enzyme activity after 30 min long reperfusion is 74, 34, 94 and 58% for the brain, renal cortex, proximal and distal sections of the small intestine, respectively. It has been found that the blood serum from ischemized rats also exerts an inhibitory effect on the enzyme activity. Application of inhibitor of the Thrombocyte-Activating Factor (TAF) BN52021 has permitted demonstrating its role in the protecting effect under conditions of Na+, K(+)-ATP-ase inhibition in the post-Ischemic period and inhibitory effect of the blood serum from ischemized rats on the enzyme activity. A conclusion is made that the TAF participated in the inhibitory effect of Na+, K(+)-ATP-ase activity in different tissues after ischemia of the small intestine of rats.

G L Vavilova - One of the best experts on this subject based on the ideXlab platform.

  • the role of the Thrombocyte Activating Factor in inhibiting na k atpase in different tissues after ischemia and reperfusion of the small intestine
    Ukrainskiĭ biokhimicheskiĭ zhurnal, 1996
    Co-Authors: G L Vavilova, O N Prokopenko, O N Kharlamova, M S Iaremenko, Vadym F. Sagach
    Abstract:

    Activity of transport Na+, K(+)-ATP-ase has been studied both in the ischemia-affected proximal section of the small intestine and in tissues which did not endure ischemia (the brain, renal cortex and the distal part of the small intestine) in dynamics 5, 30 and 60 min, after reperfusion of the ischemized small intestine of rats. It is shown that after 30 min-long ischemia of the small intestine, the Na+, K(+)-ATP-ase activity is inhibited in all the tissues mentioned. Maximal inhibition of the enzyme activity after 30 min long reperfusion is 74, 34, 94 and 58% for the brain, renal cortex, proximal and distal sections of the small intestine, respectively. It has been found that the blood serum from ischemized rats also exerts an inhibitory effect on the enzyme activity. Application of inhibitor of the Thrombocyte-Activating Factor (TAF) BN52021 has permitted demonstrating its role in the protecting effect under conditions of Na+, K(+)-ATP-ase inhibition in the post-Ischemic period and inhibitory effect of the blood serum from ischemized rats on the enzyme activity. A conclusion is made that the TAF participated in the inhibitory effect of Na+, K(+)-ATP-ase activity in different tissues after ischemia of the small intestine of rats.