Thylakoid

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Iwona Adamska - One of the best experts on this subject based on the ideXlab platform.

  • identification expression and functional analyses of a Thylakoid atp adp carrier from arabidopsis
    Journal of Biological Chemistry, 2007
    Co-Authors: Sophie Thuswaldner, Jens O. Lagerstedt, Karim Bouhidel, Christophe Der, Arti Mishra, Francis Marty, Benoît Schoefs, Marc Rojasstutz, Nathalie Leborgnecastel, Iwona Adamska
    Abstract:

    In plants the chloroplast Thylakoid membrane is the site of light-dependent photosynthetic reactions coupled to ATP synthesis. The ability of the plant cell to build and alter this membrane system is essential for efficient photosynthesis. A nucleotide translocator homologous to the bovine mitochondrial ADP/ATP carrier (AAC) was previously found in spinach Thylakoids. Here we have identified and characterized a Thylakoid ATP/ADP carrier (TAAC) from Arabidopsis.(i) Sequence homology with the bovine AAC and the prediction of chloroplast transit peptides indicated a putative carrier encoded by the At5g01500 gene, as a TAAC. (ii) Transiently expressed TAAC-green fluorescent protein fusion construct was targeted to the chloroplast. Western blotting using a peptide-specific antibody together with immunogold electron microscopy revealed a major location of TAAC in the Thylakoid membrane. Previous proteomic analyses identified this protein in chloroplast envelope preparations. (iii) Recombinant TAAC protein specifically imports ATP in exchange for ADP across the cytoplasmic membrane of Escherichia coli. Studies on isolated Thylakoids from Arabidopsis confirmed these observations. (iv) The lack of TAAC in an Arabidopsis T-DNA insertion mutant caused a 30-40% reduction in the Thylakoid ATP transport and metabolism. (v) TAAC is readily expressed in dark-grown Arabidopsis seedlings, and its level remains stable throughout the greening process. Its expression is highest in developing green tissues and in leaves undergoing senescence or abiotic stress. We propose that the TAAC protein supplies ATP for energy-dependent reactions during Thylakoid biogenesis and turnover in plants.

  • Identification, expression, and functional analyses of a Thylakoid ATP/ADP carrier from Arabidopsis
    Journal of Biological Chemistry, 2007
    Co-Authors: Sophie Thuswaldner, Jens O. Lagerstedt, Marc Rojas-stütz, Karim Bouhidel, Christophe Der, Nathalie Leborgne-castel, Arti Mishra, Francis Marty, Benoît Schoefs, Iwona Adamska
    Abstract:

    In plants the chloroplast Thylakoid membrane is the site of lightdependent photosynthetic reactions coupled to ATP synthesis. The ability of the plant cell to build and alter this membrane system is essential for efficient photosynthesis. A nucleotide translocator homologous to the bovine mitochondrial ADP/ATPcarrier (AAC) was previously found in spinach Thylakoids. Here we have identified and characterized a Thylakoid ATP/ADP carrier (TAAC) from Arabidopsis. (i) Sequence homology with the bovine AAC and the prediction of chloroplast transit peptides indicated a putative carrier encoded by the At5g01500 gene, as a TAAC. (ii) Transiently expressed TAAC-green fluorescent protein fusion construct was targeted to the chloroplast. Western blotting using a peptide-specific antibody together with immunogold electron microscopy revealed a major location of TAAC in the Thylakoid membrane. Previous proteomic analyses identified this protein in chloroplast envelope preparations. (iii) Recombinant TAAC protein specifically imports ATP in exchange for ADP across the cytoplasmic membrane of Escherichia coli. Studies on isolated Thylakoids from Arabidopsis confirmed these observations. (iv) The lack of TAAC in an Arabidopsis T-DNA insertion mutant caused a 30–40% reduction in the Thylakoid ATP transport and metabolism. (v) TAAC is readily expressed in dark-grown Arabidopsis seedlings, and its level remains stable throughout the greening process. Its expression is highest in developing green tissues and in leaves undergoing senescence or abiotic stress. We propose that the TAAC protein supplies ATP for energy-dependent reactions during Thylakoid biogenesis and turnover in plants

Sophie Thuswaldner - One of the best experts on this subject based on the ideXlab platform.

  • identification expression and functional analyses of a Thylakoid atp adp carrier from arabidopsis
    Journal of Biological Chemistry, 2007
    Co-Authors: Sophie Thuswaldner, Jens O. Lagerstedt, Karim Bouhidel, Christophe Der, Arti Mishra, Francis Marty, Benoît Schoefs, Marc Rojasstutz, Nathalie Leborgnecastel, Iwona Adamska
    Abstract:

    In plants the chloroplast Thylakoid membrane is the site of light-dependent photosynthetic reactions coupled to ATP synthesis. The ability of the plant cell to build and alter this membrane system is essential for efficient photosynthesis. A nucleotide translocator homologous to the bovine mitochondrial ADP/ATP carrier (AAC) was previously found in spinach Thylakoids. Here we have identified and characterized a Thylakoid ATP/ADP carrier (TAAC) from Arabidopsis.(i) Sequence homology with the bovine AAC and the prediction of chloroplast transit peptides indicated a putative carrier encoded by the At5g01500 gene, as a TAAC. (ii) Transiently expressed TAAC-green fluorescent protein fusion construct was targeted to the chloroplast. Western blotting using a peptide-specific antibody together with immunogold electron microscopy revealed a major location of TAAC in the Thylakoid membrane. Previous proteomic analyses identified this protein in chloroplast envelope preparations. (iii) Recombinant TAAC protein specifically imports ATP in exchange for ADP across the cytoplasmic membrane of Escherichia coli. Studies on isolated Thylakoids from Arabidopsis confirmed these observations. (iv) The lack of TAAC in an Arabidopsis T-DNA insertion mutant caused a 30-40% reduction in the Thylakoid ATP transport and metabolism. (v) TAAC is readily expressed in dark-grown Arabidopsis seedlings, and its level remains stable throughout the greening process. Its expression is highest in developing green tissues and in leaves undergoing senescence or abiotic stress. We propose that the TAAC protein supplies ATP for energy-dependent reactions during Thylakoid biogenesis and turnover in plants.

  • Identification, expression, and functional analyses of a Thylakoid ATP/ADP carrier from Arabidopsis
    Journal of Biological Chemistry, 2007
    Co-Authors: Sophie Thuswaldner, Jens O. Lagerstedt, Marc Rojas-stütz, Karim Bouhidel, Christophe Der, Nathalie Leborgne-castel, Arti Mishra, Francis Marty, Benoît Schoefs, Iwona Adamska
    Abstract:

    In plants the chloroplast Thylakoid membrane is the site of lightdependent photosynthetic reactions coupled to ATP synthesis. The ability of the plant cell to build and alter this membrane system is essential for efficient photosynthesis. A nucleotide translocator homologous to the bovine mitochondrial ADP/ATPcarrier (AAC) was previously found in spinach Thylakoids. Here we have identified and characterized a Thylakoid ATP/ADP carrier (TAAC) from Arabidopsis. (i) Sequence homology with the bovine AAC and the prediction of chloroplast transit peptides indicated a putative carrier encoded by the At5g01500 gene, as a TAAC. (ii) Transiently expressed TAAC-green fluorescent protein fusion construct was targeted to the chloroplast. Western blotting using a peptide-specific antibody together with immunogold electron microscopy revealed a major location of TAAC in the Thylakoid membrane. Previous proteomic analyses identified this protein in chloroplast envelope preparations. (iii) Recombinant TAAC protein specifically imports ATP in exchange for ADP across the cytoplasmic membrane of Escherichia coli. Studies on isolated Thylakoids from Arabidopsis confirmed these observations. (iv) The lack of TAAC in an Arabidopsis T-DNA insertion mutant caused a 30–40% reduction in the Thylakoid ATP transport and metabolism. (v) TAAC is readily expressed in dark-grown Arabidopsis seedlings, and its level remains stable throughout the greening process. Its expression is highest in developing green tissues and in leaves undergoing senescence or abiotic stress. We propose that the TAAC protein supplies ATP for energy-dependent reactions during Thylakoid biogenesis and turnover in plants

Ute C Vothknecht - One of the best experts on this subject based on the ideXlab platform.

  • Chloroplast Ca2+ fluxes into and across Thylakoids revealed by Thylakoid-targeted aequorin probes
    Plant Physiology, 2018
    Co-Authors: Simone Sello, Roberto Moscatiello, Norbert Mehlmer, Manuela Leonardelli, Luca Carraretto, Enrico Cortese, Filippo Zanella, Barbara Baldan, Ildikò Szabò, Ute C Vothknecht
    Abstract:

    Chloroplasts require a fine-tuned control of their internal Ca2+ concentration, which is crucial for many aspects of photosynthesis and for other chloroplast-localized processes. Increasing evidence suggests that calcium regulation within chloroplasts also may influence Ca2+ signaling pathways in the cytosol. To investigate the involvement of Thylakoids in Ca2+ homeostasis and in the modulation of chloroplast Ca2+ signals in vivo, we targeted the bioluminescent Ca2+ reporter aequorin as a YFP fusion to the lumen and the stromal surface of Thylakoids in Arabidopsis (Arabidopsis thaliana). Thylakoid localization of aequorin-based probes in stably transformed lines was confirmed by confocal microscopy, immunogold labeling, and biochemical analyses. In resting conditions in the dark, free Ca2+ levels in the Thylakoid lumen were maintained at about 0.5 μm, which was a 3- to 5-fold higher concentration than in the stroma. Monitoring of chloroplast Ca2+ dynamics in different intrachloroplast subcompartments (stroma, Thylakoid membrane, and Thylakoid lumen) revealed the occurrence of stimulus-specific Ca2+ signals, characterized by unique kinetic parameters. Oxidative and salt stresses initiated pronounced free Ca2+ changes in the Thylakoid lumen. Localized Ca2+ increases also were observed on the Thylakoid membrane surface, mirroring transient Ca2+ changes observed for the bulk stroma, but with specific Ca2+ dynamics. Moreover, evidence was obtained for dark-stimulated intraThylakoid Ca2+ changes, suggesting a new scenario for light-to-dark-induced Ca2+ fluxes inside chloroplasts. Hence, Thylakoid-targeted aequorin reporters can provide new insights into chloroplast Ca2+ storage and signal transduction. These probes represent novel tools with which to investigate the role of Thylakoids in Ca2+ signaling networks within chloroplasts and plant cells.

  • vipp1 a nuclear gene of arabidopsis thaliana essential for Thylakoid membrane formation
    Proceedings of the National Academy of Sciences of the United States of America, 2001
    Co-Authors: Daniela Kroll, Karin Meierhoff, Nicole Bechtold, Mikio Kinoshita, Sabine Westphal, Ute C Vothknecht, Jurgen Soll, Peter Westhoff
    Abstract:

    The conversion of light to chemical energy by the process of photosynthesis is localized to the Thylakoid membrane network in plant chloroplasts. Although several pathways have been described that target proteins into and across the Thylakoids, little is known about the origin of this membrane system or how the lipid backbone of the Thylakoids is transported and fused with the target membrane. Thylakoid biogenesis and maintenance seem to involve the flow of membrane elements via vesicular transport. Here we show by mutational analysis that deletion of a single gene called VIPP1 (vesicle-inducing protein in plastids 1) is deleterious to Thylakoid membrane formation. Although VIPP1 is a hydrophilic protein it is found in both the inner envelope and the Thylakoid membranes. In VIPP1 deletion mutants vesicle formation is abolished. We propose that VIPP1 is essential for the maintenance of Thylakoids by a transport pathway not previously recognized.

  • vipp1 deletion mutant of synechocystis a connection between bacterial phage shock and Thylakoid biogenesis
    Proceedings of the National Academy of Sciences of the United States of America, 2001
    Co-Authors: Sabine Westphal, Jurgen Soll, Lisa Heins, Ute C Vothknecht
    Abstract:

    Plant chloroplasts originated from an endosymbiotic event by which an ancestor of contemporary cyanobacteria was engulfed by an early eukaryotic cell and then transformed into an organelle. Oxygenic photosynthesis is the specific feature of cyanobacteria and chloroplasts, and the photosynthetic machinery resides in an internal membrane system, the Thylakoids. The origin and genesis of Thylakoid membranes, which are essential for oxygenic photosynthesis, are still an enigma. Vipp1 (vesicle-inducing protein in plastids 1) is a protein located in both the inner envelope and the Thylakoids of Pisum sativum and Arabidopsis thaliana. In Arabidopsis disruption of the VIPP1 gene severely affects the plant's ability to form properly structured Thylakoids and as a consequence to carry out photosynthesis. In contrast, Vipp1 in Synechocystis appears to be located exclusively in the plasma membrane. Yet, as in higher plants, disruption of the VIPP1 gene locus leads to the complete loss of Thylakoid formation. So far VIPP1 genes are found only in organisms carrying out oxygenic photosynthesis. They share sequence homology with a subunit encoded by the bacterial phage shock operon (PspA) but differ from PspA by a C-terminal extension of about 30 amino acids. In two cyanobacteria, Synechocystis and Anabaena, both a VIPP1 and a pspA gene are present, and phylogenetic analysis indicates that VIPP1 originated from a gene duplication of the latter and thereafter acquired its new function. It also appears that the C-terminal extension that discriminates VIPP1 proteins from PspA is important for its function in Thylakoid formation.

Arti Mishra - One of the best experts on this subject based on the ideXlab platform.

  • identification expression and functional analyses of a Thylakoid atp adp carrier from arabidopsis
    Journal of Biological Chemistry, 2007
    Co-Authors: Sophie Thuswaldner, Jens O. Lagerstedt, Karim Bouhidel, Christophe Der, Arti Mishra, Francis Marty, Benoît Schoefs, Marc Rojasstutz, Nathalie Leborgnecastel, Iwona Adamska
    Abstract:

    In plants the chloroplast Thylakoid membrane is the site of light-dependent photosynthetic reactions coupled to ATP synthesis. The ability of the plant cell to build and alter this membrane system is essential for efficient photosynthesis. A nucleotide translocator homologous to the bovine mitochondrial ADP/ATP carrier (AAC) was previously found in spinach Thylakoids. Here we have identified and characterized a Thylakoid ATP/ADP carrier (TAAC) from Arabidopsis.(i) Sequence homology with the bovine AAC and the prediction of chloroplast transit peptides indicated a putative carrier encoded by the At5g01500 gene, as a TAAC. (ii) Transiently expressed TAAC-green fluorescent protein fusion construct was targeted to the chloroplast. Western blotting using a peptide-specific antibody together with immunogold electron microscopy revealed a major location of TAAC in the Thylakoid membrane. Previous proteomic analyses identified this protein in chloroplast envelope preparations. (iii) Recombinant TAAC protein specifically imports ATP in exchange for ADP across the cytoplasmic membrane of Escherichia coli. Studies on isolated Thylakoids from Arabidopsis confirmed these observations. (iv) The lack of TAAC in an Arabidopsis T-DNA insertion mutant caused a 30-40% reduction in the Thylakoid ATP transport and metabolism. (v) TAAC is readily expressed in dark-grown Arabidopsis seedlings, and its level remains stable throughout the greening process. Its expression is highest in developing green tissues and in leaves undergoing senescence or abiotic stress. We propose that the TAAC protein supplies ATP for energy-dependent reactions during Thylakoid biogenesis and turnover in plants.

  • Identification, expression, and functional analyses of a Thylakoid ATP/ADP carrier from Arabidopsis
    Journal of Biological Chemistry, 2007
    Co-Authors: Sophie Thuswaldner, Jens O. Lagerstedt, Marc Rojas-stütz, Karim Bouhidel, Christophe Der, Nathalie Leborgne-castel, Arti Mishra, Francis Marty, Benoît Schoefs, Iwona Adamska
    Abstract:

    In plants the chloroplast Thylakoid membrane is the site of lightdependent photosynthetic reactions coupled to ATP synthesis. The ability of the plant cell to build and alter this membrane system is essential for efficient photosynthesis. A nucleotide translocator homologous to the bovine mitochondrial ADP/ATPcarrier (AAC) was previously found in spinach Thylakoids. Here we have identified and characterized a Thylakoid ATP/ADP carrier (TAAC) from Arabidopsis. (i) Sequence homology with the bovine AAC and the prediction of chloroplast transit peptides indicated a putative carrier encoded by the At5g01500 gene, as a TAAC. (ii) Transiently expressed TAAC-green fluorescent protein fusion construct was targeted to the chloroplast. Western blotting using a peptide-specific antibody together with immunogold electron microscopy revealed a major location of TAAC in the Thylakoid membrane. Previous proteomic analyses identified this protein in chloroplast envelope preparations. (iii) Recombinant TAAC protein specifically imports ATP in exchange for ADP across the cytoplasmic membrane of Escherichia coli. Studies on isolated Thylakoids from Arabidopsis confirmed these observations. (iv) The lack of TAAC in an Arabidopsis T-DNA insertion mutant caused a 30–40% reduction in the Thylakoid ATP transport and metabolism. (v) TAAC is readily expressed in dark-grown Arabidopsis seedlings, and its level remains stable throughout the greening process. Its expression is highest in developing green tissues and in leaves undergoing senescence or abiotic stress. We propose that the TAAC protein supplies ATP for energy-dependent reactions during Thylakoid biogenesis and turnover in plants

Jens O. Lagerstedt - One of the best experts on this subject based on the ideXlab platform.

  • identification expression and functional analyses of a Thylakoid atp adp carrier from arabidopsis
    Journal of Biological Chemistry, 2007
    Co-Authors: Sophie Thuswaldner, Jens O. Lagerstedt, Karim Bouhidel, Christophe Der, Arti Mishra, Francis Marty, Benoît Schoefs, Marc Rojasstutz, Nathalie Leborgnecastel, Iwona Adamska
    Abstract:

    In plants the chloroplast Thylakoid membrane is the site of light-dependent photosynthetic reactions coupled to ATP synthesis. The ability of the plant cell to build and alter this membrane system is essential for efficient photosynthesis. A nucleotide translocator homologous to the bovine mitochondrial ADP/ATP carrier (AAC) was previously found in spinach Thylakoids. Here we have identified and characterized a Thylakoid ATP/ADP carrier (TAAC) from Arabidopsis.(i) Sequence homology with the bovine AAC and the prediction of chloroplast transit peptides indicated a putative carrier encoded by the At5g01500 gene, as a TAAC. (ii) Transiently expressed TAAC-green fluorescent protein fusion construct was targeted to the chloroplast. Western blotting using a peptide-specific antibody together with immunogold electron microscopy revealed a major location of TAAC in the Thylakoid membrane. Previous proteomic analyses identified this protein in chloroplast envelope preparations. (iii) Recombinant TAAC protein specifically imports ATP in exchange for ADP across the cytoplasmic membrane of Escherichia coli. Studies on isolated Thylakoids from Arabidopsis confirmed these observations. (iv) The lack of TAAC in an Arabidopsis T-DNA insertion mutant caused a 30-40% reduction in the Thylakoid ATP transport and metabolism. (v) TAAC is readily expressed in dark-grown Arabidopsis seedlings, and its level remains stable throughout the greening process. Its expression is highest in developing green tissues and in leaves undergoing senescence or abiotic stress. We propose that the TAAC protein supplies ATP for energy-dependent reactions during Thylakoid biogenesis and turnover in plants.

  • Identification, expression, and functional analyses of a Thylakoid ATP/ADP carrier from Arabidopsis
    Journal of Biological Chemistry, 2007
    Co-Authors: Sophie Thuswaldner, Jens O. Lagerstedt, Marc Rojas-stütz, Karim Bouhidel, Christophe Der, Nathalie Leborgne-castel, Arti Mishra, Francis Marty, Benoît Schoefs, Iwona Adamska
    Abstract:

    In plants the chloroplast Thylakoid membrane is the site of lightdependent photosynthetic reactions coupled to ATP synthesis. The ability of the plant cell to build and alter this membrane system is essential for efficient photosynthesis. A nucleotide translocator homologous to the bovine mitochondrial ADP/ATPcarrier (AAC) was previously found in spinach Thylakoids. Here we have identified and characterized a Thylakoid ATP/ADP carrier (TAAC) from Arabidopsis. (i) Sequence homology with the bovine AAC and the prediction of chloroplast transit peptides indicated a putative carrier encoded by the At5g01500 gene, as a TAAC. (ii) Transiently expressed TAAC-green fluorescent protein fusion construct was targeted to the chloroplast. Western blotting using a peptide-specific antibody together with immunogold electron microscopy revealed a major location of TAAC in the Thylakoid membrane. Previous proteomic analyses identified this protein in chloroplast envelope preparations. (iii) Recombinant TAAC protein specifically imports ATP in exchange for ADP across the cytoplasmic membrane of Escherichia coli. Studies on isolated Thylakoids from Arabidopsis confirmed these observations. (iv) The lack of TAAC in an Arabidopsis T-DNA insertion mutant caused a 30–40% reduction in the Thylakoid ATP transport and metabolism. (v) TAAC is readily expressed in dark-grown Arabidopsis seedlings, and its level remains stable throughout the greening process. Its expression is highest in developing green tissues and in leaves undergoing senescence or abiotic stress. We propose that the TAAC protein supplies ATP for energy-dependent reactions during Thylakoid biogenesis and turnover in plants